Michael B. Eisen

Developmental roles of 21 Drosophilatranscription factors are determined by quantitative differences in binding to an overlapping set of thousands of genomic regions (2009)

MacArthur, Stewart, Li, Xiao-Yong, Li, Jingyi, Brown, James B, Chu, Hou Cheng, Zeng, Lucy, ...

Abstract Background We previously established that six sequence-specific transcription factors that initiate anterior/posterior patterning in Drosophila bind to overlapping sets of thousands of...

A condensin-like dosage compensation complex acts at a distance to control expression throughout the genome (2009)

Jans, Judith, Gladden, John M., Ralston, Edward J., Pickle, Catherine S., Michel, Agnès H., Pferdehirt, Rebecca R., ...

In many species, a dosage compensation complex (DCC) is targeted to X chromosomes of one sex to equalize levels of X-gene products between males (1X) and females (2X). Here we identify cis-acting...

PointCloudXplore: A Visualization Tool for 3D Gene Expression Data (2008)

Oliver Rübel, Gunther H. Weber, Charless C. Fowlkes, Lisa Simirenko, ...

a suite of methods that support quantitative, computational analysis of threedimensional (3D) gene expression patterns with cellular resolution in early Drosophila embryos, aiming at a more in-depth...

BMC Cell Biology (2008)

Hanchuan Peng, Fuhui Long, Jie Zhou, Garmay Leung, Michael B Eisen

Automatic image analysis for gene expression patterns of fly embryos

Determining physical constraints in transcriptional initiation complexes using DNA sequence analysis (2008)

Shultzaberger, Ryan K., Chiang, Derek Y., Moses, Alan M., Eisen, Michael B.

Eukaryotic gene expression is often under the control of cooperatively acting transcription factors whose binding is limited by structural constraints. By determining these structural constraints, we...

Design of a combinatorial dna microarray for protein-dna interaction studies (2008)

Mintseris, Julian, Eisen, Michael B.

Background: Discovery of precise specificity of transcription factors is an important step on the way to understanding the complex mechanisms of gene regulation in eukaryotes. Recently,...

Aging and Gene Expression in the Primate (2008)

Hunter B. Fraser, Joshua B. Plotkin, Svante Pääbo, Michael B. Eisen

It is well established that gene expression levels in many organisms change during the aging process, and the advent of DNA microarrays has allowed genome-wide patterns of transcriptional changes...

¶ To whom correspondence should be addressed. E-mail: (2008)

P. Wall, Aaron E. Hirsh, Hunter B. Fraser, Guri Giaever, Michael B. Eisen, Marcus W. Feldman

Running head: yeast functional genomics and evolutionary rate Background Statistical noise and inaccuracy in functional genomic estimates of protein dispensability and evolutionary rate present...

Transcription Factors Bind Thousands of Active and Inactive Regions in the Drosophila Blastoderm (2008)

Xiao-yong Li, Stewart MacArthur, Richard Bourgon, David Nix, Daniel A. Pollard, Venky N. Iyer, ...

Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to...

Constructing a quantitative spatio-temporal atlas of gene expression in Drosophila blastoderm. to appear in: Cell (Accepted (2008)

Charless C. Fowlkes, Gunther H. Weber, Oliver Rübel, Min-yu Huang, ...

To fully understand animal transcription networks, it is essential to accurately measure the spatial and temporal expression patterns of transcription factors and their targets. We describe a...

with (2007)

Trevor Hastie, Robert Tibshirani, Michael B Eisen, Ash Alizadeh, Ronald Levy, Louis Staudt, ...

shaving ’ as a method for identifying distinct sets of genes

BIOINFORMATICS Vol. 18 no. 9 2002 (2007)

Michael B. Eisen

Identification of regulatory elements using a feature selection method Sund uz Keles

through fuzzy k-means (2007)

Audrey P Gasch, Michael B Eisen

the conditional coregulation of yeast gene expression

1 (2007)

Derek Y. Chiang, Patrick O. Brown, Michael B. Eisen

Visualizing associations between genome sequences and gene expression data using genome-mean expression profiles

Noise Minimization in Eukaryotic Gene Expression (2007)

Hunter B. Fraser, Aaron E. Hirsh, Guri Giaever, Jochen Kumm, Michael B. Eisen

All organisms have elaborate mechanisms to control rates of protein production. However, protein production is also subject to stochastic fluctuations, or ‘‘noise.’ ’ Several recent studies...

BMC Bioinformatics BioMed Central Research article Benchmarking tools for the alignment of functional noncoding DNA (2007)

Daniel A Pollard, Jens Stoye, Susan E Celniker, Michael B Eisen, Open Access

© 2004 Pollard et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this...

Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures (2007)

Lin, Michael F, Kheradpour, Pouya, Pedersen, Jakob S, Parts, Leopold, Carlson, Joseph W, ...

Sequencing of multiple related species followed by comparative genomics analysis constitutes a powerful approach for the systematic understanding of any genome. Here, we use the genomes of 12...

Automatic image analysis for gene expression patterns of fly embryos (2007)

Peng, Hanchuan, Long, Fuhui, Zhou, Jie, Leung, Garmay, Eisen, Michael B, Myers, Eugene W

Abstract Background Staining the m RNA of a gene via in situ hybridization (ISH) during the development of a D. melanogaster embryo delivers the detailed spatio-temporal pattern of expression of the...

Three-dimensional morphology and gene expression in the Drosophilablastoderm at cellular resolution I: data acquisition pipeline (2006)

Luengo Hendriks, Cris L, Keränen, Soile VE, Fowlkes, Charless C, Simirenko, Lisa, Weber, Gunther H, DePace, Angela H, ...

Abstract Background To model and thoroughly understand animal transcription networks, it is essential to derive accurate spatial and temporal descriptions of developing gene expression patterns with...

Design of a combinatorial DNA microarray for protein-DNA interaction studies (2006)

Mintseris, Julian, Eisen, Michael B

Abstract Background Discovery of precise specificity of transcription factors is an important step on the way to understanding the complex mechanisms of gene regulation in eukaryotes. Recently,...

Large-Scale Turnover of Functional Transcription Factor Binding Sites in Drosophila (2006)

Alan M. Moses, Daniel A. Pollard, David A. Nix, Venky N. Iyer, Xiao-Yong Li, Mark D. Biggin, ...

The gain and loss of functional transcription factor binding sites has been proposed as a major source of evolutionary change in cis-regulatory DNA and gene expression. We have developed an...

Widespread Discordance of Gene Trees with Species Tree in Drosophila: Evidence for Incomplete Lineage Sorting (2006)

Daniel A. Pollard, Venky N. Iyer, Alan M. Moses, Michael B. Eisen

The phylogenetic relationship of the now fully sequenced species Drosophila erecta and D. yakuba with respect to the D. melanogaster species complex has been a subject of controversy. All three...

Insights into social insects from the genome of the honeybee Apis mellifera (2006)

Robinson, Gene E, Gibbs, Richard A, Worley, Kim C, Evans, Jay D, Maleszka, Ryszard, ...

Here we report the genome sequence of the honeybee Apis mellifera, a key model for social behaviour and essential to global ecology through pollination. Compared with other sequenced insect genomes,...

Widespread Discordance of Gene Trees with Species Tree in Drosophila: Evidence for Incomplete Lineage Sorting (2006)

Pollard, Daniel A., Iyer, Venky N., Moses, Alan M., Eisen, Michael B.

The phylogenetic relationship of the now fully sequenced species Drosophila erecta and D. yakuba with respect to the D. melanogaster species complex has been a subject of controversy. All three...

Detecting the limits of regulatory element conservation and divergence estimation using pairwise and multiple alignments (2006)

Pollard, Daniel A, Moses, Alan M, Iyer, Venky N, Eisen, Michael B

Abstract Background Molecular evolutionary studies of noncoding sequences rely on multiple alignments. Yet how multiple alignment accuracy varies across sequence types, tree topologies, divergences...

Detecting the limits of regulatory element conservation and divergence estimation using pairwise and multiple alignments (2006)

Pollard, Daniel A., Moses, Alan M., Iyer, Venky N., Eisen, Michael B.

Background: Molecular evolutionary studies of noncoding sequences rely on multiple alignments. Yet how multiple alignment accuracy varies across sequence types, tree topologies, divergences and...

Primate-specific evolution of an LDLR enhancer (2006)

Wang, Qian-fei, Prabhakar, Shyam, Wang, Qianben, Moses, Alan M, Chanan, Sumita, Brown, Myles, ...

Background Sequence changes in regulatory regions have often been invoked to explain phenotypic divergence among species, but molecular examples of this have been difficult to obtain. Results In this...

Large-scale turnover of functional transcription factor binding sites in Drosophila (2006)

Alan M. Moses, Daniel Pollard, David A Nix, Venky N. Iyer, Xiao-Yong Li, Mark D Biggin, ...

The gain and loss of functional transcription-factor binding sites has been proposed as a major source of evolutionary change in cis-regulatory DNA and gene expression. We have developed an...

Whole Genome Phylogeny of the Drosophila melanogaster Species Subgroup: Widespread Discordance with Species Tree & Evidence for Incomplete Lineage Sorting (2006)

Daniel Pollard, Venky N. Iyer, Alan M. Moses, Michael B. Eisen

Understanding the basis for incongruence of phylogenies constructed from different genes or loci and how to properly handle such disaccord is an important challenge to evolutionary biology. To...

Large-scale turnover of functional transcription factor binding sites in Drosophila (2006)

Moses, Alan M., Pollard, Daniel A., Nix, David A., Iyer, Venky N., Li, Xiao-Yong, Biggin, Mark D., ...

The gain and loss of functional transcription-factor binding sites has been proposed as a major source of evolutionary change in cis-regulatory DNA and gene expression. We have developed an...

Primate-Specific Evolution of an LDLR Enhancer (2006)

Wang, Qian-fei, Prabhakar, Shyam, Wang, Qianben, Moses, Alan M., Chanan, Sumita, Brown, Myles, ...

Sequence changes in regulatory regions have often been invoked to explain phenotypic divergence among species, but molecular examples of this have been difficult to obtain. In this study, we...

Flexible promoter architecture requirements for coactivator recruitment (2006)

Chiang, Derek Y, Nix, David A, Shultzaberger, Ryan K, Gasch, Audrey P, Eisen, Michael B

Abstract Background The spatial organization of transcription factor binding sites in regulatory DNA, and the composition of intersite sequences, influences the assembly of the multiprotein complexes...

In Vivo Enhancer Analysis Chromosome 16 Conserved Noncoding Sequences (2006)

Pennacchio, Len A., Ahituv, Nadav, Moses, Alan M., Nobrega, Marcelo, Prabhakar, Shyam, Shoukry, Malak, ...

The identification of enhancers with predicted specificities in vertebrate genomes remains a significant challenge that is hampered by a lack of experimentally validated training sets. In this study,...

Open Access (2006)

Soile Ve Keränen, Charless C Fowlkes, Lisa Simirenko, Gunther H Weber, Angela H Depace, ...

Three-dimensional morphology and gene expression in the Drosophila blastoderm at cellular resolution I: data acquisition pipeline

BioMed Central (2006)

Bmc Bioinformatics, Daniel A Pollard, Alan M Moses, Venky N Iyer, Michael B Eisen, Michael B Eisen

Research article Detecting the limits of regulatory element conservation and divergence estimation using pairwise and multiple alignments

Large-scale turnover of functional transcription factor binding sites in Drosophila (2006)

Alan M. Moses, Daniel A. Pollard, David A. Nix, Venky N. Iyer, Xiao-yong Li, Mark D. Biggin, ...

The gain and loss of functional transcription factor binding sites has been proposed as a major source of evolutionary change in cis-regulatory DNA and gene expression. We have developed an...

Article type Methodology article (2006)

Bmc Bioinformatics, Michael B Eisen, Julian Mintseris, Michael B. Eisen

This Provisional PDF corresponds to the article as it appeared upon acceptance. Copyedited and fully formatted PDF and full text (HTML) versions will be made available soon. Design of a combinatorial...

BMC Molecular Biology (2006)

Derek Y Chiang, David A Nix, Ryan K Shultzaberger, Audrey P Gasch, Michael B Eisen, Derek Y Chiang, ...

This Provisional PDF corresponds to the article as it appeared upon acceptance. The fully-formatted PDF version will become available shortly after the date of publication, from the URL listed below....

Rapid quantitative profiling of complex microbial populations (2006)

Palmer, Chana, Bik, Elisabeth M., Eisen, Michael B., Eckburg, Paul B., Sana, Theodore R., Wolber, Paul K., ...

Diverse and complex microbial ecosystems are found in virtually every environment on earth, yet we know very little about their composition and ecology. Comprehensive identification and...

BioMed Central (2006)

Bmc Bioinformatics, Daniel A Pollard, Alan M Moses, Venky N Iyer, Michael B Eisen, Michael B Eisen

Research article Detecting the limits of regulatory element conservation and divergence estimation using pairwise and multiple alignments

Aging and Gene Expression in the Primate Brain (2005)

Hunter B. Fraser, Philipp Khaitovich, Joshua B. Plotkin, Svante Pääbo, Michael B. Eisen

Transcriptional profiles in human and chimpanzee reveal a diversity of aging patterns present within the human brain, as well as how rapidly genome-wide patterns of aging can evolve between species.

Aging and Gene Expression in the Primate Brain (2005)

Hunter B. Fraser, Philipp Khaitovich, Joshua B. Plotkin, Svante Pääbo, Michael B. Eisen

It is well established that gene expression levels in many organisms change during the aging process, and the advent of DNA microarrays has allowed genome-wide patterns of transcriptional changes...

All motifs are NOT created equal: structural properties of transcription factor-DNA interactions and the inference of sequence specificity (2005)

Eisen, Michael B

Abstract The identification of transcription factor binding sites in genome sequences is an important problem in contemporary sequence analysis, and a plethora of approaches to the problem have been...

Serendipitous discovery of Wolbachiagenomes in multiple Drosophilaspecies (2005)

Salzberg, Steven L, Hotopp, Julie, Delcher, Arthur L, Pop, Mihai, Smith, Douglas R, Eisen, Michael B, ...

Abstract Background The Trace Archive is a repository for the raw, unanalyzed data generated by large-scale genome sequencing projects. The existence of this data offers scientists the possibility of...

Aging and Gene Expression in the Primate Brain (2005)

Fraser, Hunter B., Khaitovich, Philipp, Plotkin, Joshua B., Paabo, Svante, Eisen, Michael B.

It is well established that gene expression levels in many organisms change during the aging process, and the advent of DNA microarrays has allowed genome-wide patterns of transcriptional changes...

GATA: a graphic alignment tool for comparative sequence analysis (2005)

Nix, David A, Eisen, Michael B

Abstract Background Several problems exist with current methods used to align DNA sequences for comparative sequence analysis. Most dynamic programming algorithms assume that conserved sequence...

Estimating Motifs Under Order Restrictions (2005)

Van Zwet, Erik W, Kechris, Katherina J, Bickel, Peter J, Eisen , Michael B.

Transcription factors and many other DNA-binding proteins recognize more than one specific sequence. Among sequences recognized by a given DNA-binding protein, different positions exhibit varying...

Estimating Motifs Under Order Restrictions (2005)

Van Zwet, Erik W, Kechris, Katherina J, Bickel, Peter J, Eisen , Michael B.

Transcription factors and many other DNA-binding proteins recognize more than one specific sequence. Among sequences recognized by a given DNA-binding protein, different positions exhibit varying...

Estimating Motifs Under Order Restrictions (2005)

Van Zwet, Erik W, Kechris, Katherina J, Bickel, Peter J, Eisen , Michael B.

Transcription factors and many other DNA-binding proteins recognize more than one specific sequence. Among sequences recognized by a given DNA-binding protein, different positions exhibit varying...

Estimating Motifs Under Order Restrictions (2005)

Van Zwet, Erik W, Kechris, Katherina J, Bickel, Peter J, Eisen , Michael B.

Transcription factors and many other DNA-binding proteins recognize more than one specific sequence. Among sequences recognized by a given DNA-binding protein, different positions exhibit varying...

GATA: A graphic alignment tool for comparative sequence analysis (2005)

Nix, David A., Eisen, Michael B.

Several problems exist with current methods used to align DNA sequences for comparative sequence analysis. Most dynamic programming algorithms assume that conserved sequence elements are collinear....

Aging and Gene Expression in the Primate Brain (2005)

Fraser, Hunter B., Khaitovich, Philipp, Plotkin, Joshua B., Pääbo, Svante, Eisen, Michael B.

It is well established that gene expression levels in many organisms change during the aging process, and the advent of DNA microarrays has allowed genome-wide patterns of transcriptional changes...

Serendipitous discovery of Wolbachia genomes in multiple Drosophila species (2005)

Salzberg, Steven L., Dunning Hotopp, Julie C., Delcher, Arthur L., Pop, Mihai, Smith, Douglas R, Eisen, Michael B., ...

Background: The Trace Archive is a repository for the raw, unanalyzed data generated by largescale genome sequencing projects. The existence of this data offers scientists the possibility of...

Serendipitous discovery of Wolbachia genomes in multiple Drosophila species (2005)

Salzberg, Steven L., Dunning Hotopp, Julie C., Delcher, Arthur L., Pop, Mihai, Smith, Douglas R, Eisen, Michael B., ...

Background: The Trace Archive is a repository for the raw, unanalyzed data generated by largescale genome sequencing projects. The existence of this data offers scientists the possibility of...

Rapid quantitative profiling of complex microbial populations (2005)

Chana Palmer, Elisabeth M. Bik, Michael B. Eisen, Paul B. Eckburg, Theodore R. Sana, Paul K. Wolber, ...

Diverse and complex microbial ecosystems are found in virtually every environment on earth, yet we know very little about their composition and ecology. Comprehensive identification and...

the Inference of Sequence Specificity (2005)

Michael B Eisen, Michael B. Eisen, Michael B. Eisen

This information has not been peer-reviewed. Responsibility for the findings rests solely with the author(s).

Conservation and Evolution of Cis-Regulatory Systems in Ascomycete Fungi (2004)

Audrey P. Gasch, Alan M. Moses, Derek Y. Chiang, Hunter B. Fraser, Mark Berardini, Michael B. Eisen

A systematic examination of the gene regulatory elements in ascomycete fungi reveals striking conservation along with some examples of the ways in which regulatory systems can evolve.

Conservation and Evolution of Cis-Regulatory Systems in Ascomycete Fungi (2004)

Audrey P. Gasch, Alan M. Moses, Derek Y. Chiang, Hunter B. Fraser, Mark Berardini, Michael B. Eisen

Relatively little is known about the mechanisms through which gene expression regulation evolves. To investigate this, we systematically explored the conservation of regulatory networks in fungi by...

MONKEY: identifying conserved transcription-factor binding sites in multiple alignments using a binding site-specific evolutionary model (2004)

Moses, Alan M, Chiang, Derek Y, Pollard, Daniel A, Iyer, Venky N, Eisen, Michael B

Abstract We introduce a method (MONKEY) to identify conserved transcription-factor binding sites in multispecies alignments. MONKEY employs probabilistic models of factor specificity and binding-site...

Computational identification of developmental enhancers: conservation and function of transcription factor binding-site clusters in Drosophila melanogasterand Drosophila pseudoobscura (2004)

Berman, Benjamin P, Pfeiffer, Barret D, Laverty, Todd R, Salzberg, Steven L, Rubin, Gerald M, Eisen, Michael B, ...

Abstract Background The identification of sequences that control transcription in metazoans is a major goal of genome analysis. In a previous study, we demonstrated that searching for clusters of...

Detecting DNA regulatory motifs by incorporating positional trends in information content (2004)

Kechris, Katherina J, Van Zwet, Erik, Bickel, Peter J, Eisen, Michael B

Abstract On the basis of the observation that conserved positions in transcription factor binding sites are often clustered together, we propose a simple extension to the model-based motif discovery...

Noise Minimization in Eukaryotic Gene Expression (2004)

Hunter B. Fraser, Aaron E. Hirsh, Guri Giaever, Jochen Kumm, Michael B. Eisen

Analysis of gene expression data for nearly every gene in yeast provides evidence that random variation in the production rate of proteins could significantly affect the fitness of an organism.

Noise Minimization in Eukaryotic Gene Expression (2004)

Hunter B. Fraser, Aaron E. Hirsh, Guri Giaever, Jochen Kumm, Michael B. Eisen

All organisms have elaborate mechanisms to control rates of protein production. However, protein production is also subject to stochastic fluctuations, or “noise.” Several recent studies in...

Detecting DNA regulatory motifs by incorporating positional trends in information content (2004)

Kechris, Katherina J., Van Zwet, Erik, Bickel, Peter J., Eisen, Michael B.

On the basis of the observation that conserved positions in transcription factor binding sites are often clustered together, we propose a simple extension to the model-based motif discovery methods....

Population genetic variation in gene expression is associated with phenotypic variation in Saccharomyces cerevisiae (2004)

Fay, Justin C, McCullough, Heather L, Sniegowski, Paul D, Eisen, Michael B

Abstract Background The relationship between genetic variation in gene expression and phenotypic variation observable in nature is not well understood. Identifying how many phenotypes are associated...

Coevolution of gene expression among interacting proteins (2004)

Fraser, Hunter B., Hirsh, Aaron E., Wall, Dennis P., Eisen, Michael B.

Physically interacting proteins or parts of proteins are expected to evolve in a coordinated manner that preserves proper interactions. Such coevolution at the amino acid-sequence level is well...

Population genetic variation in gene expression is associated with phenotypic variation in Saccharomyces cerevisiae (2004)

Fay, Justin C., McCullough, Heather L., Sniegowski, Paul D., Eisen, Michael B.

Background: The relationship between genetic variation in gene expression and phenotypic variation observable in nature is not well understood. Identifying how many phenotypes are associated with...

Benchmarking tools for the alignment of functional noncoding DNA (2004)

Pollard, Daniel A, Bergman, Casey M, Stoye, Jens, Celniker, Susan E, Eisen, Michael B

Abstract Background Numerous tools have been developed to align genomic sequences. However, their relative performance in specific applications remains poorly characterized. Alignments of...

Benchmarking tools for the alignment of functional noncoding DNA (2004)

Daniel A. Pollard, Jens Stoye, E. Celniker, Michael B. Eisen, Cb Eh

* corresponding author. Background Numerous tools have been developed to align genomic sequences. However, their relative performance in specific applications remains poorly characterized. Alignments...

clusters in Drosophila melanogaster and Drosophila (2004)

Benjamin P Berman, Barret D Pfeiffer, Todd R Laverty, Steven L Salzberg, Gerald M Rubin, Michael B Eisen, ...

Computational identification of developmental enhancers: conservation and function of transcription factor binding-site

Open Access Method (2004)

Alan M Moses, Derek Y Chiang, Daniel A Pollard, Venky N Iyer, Michael B Eisen

MONKEY: identifying conserved transcription-factor binding sites in multiple alignments using a binding site-specific evolutionary model

Conservation and evolution of cis-regulatory systems in ascomycete fungi (2004)

Audrey P. Gasch, Alan M. Moses, Derek Y. Chiang, Hunter B. Fraser, Mark Berardini, Michael B. Eisen

Relatively little is known about the mechanisms through which gene expression regulation evolves. To investigate this, we systematically explored the conservation of regulatory networks in fungi by...

Why PLoS Became a Publisher (2003)

Patrick O. Brown, Michael B. Eisen, Harold E. Varmus

Public Library of Science has grown from a grassroots movement to a nonprofit publisher, in order to catalyze change towards open-access publishing of the scientific literature.

Position specific variation in the rate of evolution in transcription factor binding sites (2003)

Moses, Alan M., Chiang, Derek Y., Kellis, Manolis, Lander, Eric S., Eisen, Michael B.

Background: The binding sites of sequence specific transcription factors are an important and relatively well-understood class of functional non-coding DNAs. Although a wide variety of experimental...

Position specific variation in the rate of evolution in transcription factor binding sites (2003)

Moses, Alan M, Chiang, Derek Y, Kellis, Manolis, Lander, Eric S, Eisen, Michael B

Abstract Background The binding sites of sequence specific transcription factors are an important and relatively well-understood class of functional non-coding DNAs. Although a wide variety of...

Supervised Detection of Regulatory Motifs in DNA Sequences (2003)

Keles, Sunduz, Van Der Laan, Mark J., Dudoit, Sandrine, Xing, Biao, Eisen , Michael B.

Identification of transcription factor binding sites (regulatory motifs) is a major interest in contemporary biology. We propose a new likelihood based method, COMODE, for identifying structural...

Supervised Detection of Regulatory Motifs in DNA Sequences (2003)

Keles, Sunduz, Van Der Laan, Mark J., Dudoit, Sandrine, Xing, Biao, Eisen , Michael B.

Identification of transcription factor binding sites (regulatory motifs) is a major interest in contemporary biology. We propose a new likelihood based method, COMODE, for identifying structural...

Supervised Detection of Regulatory Motifs in DNA Sequences (2003)

Keles, Sunduz, Van Der Laan, Mark J., Dudoit, Sandrine, Xing, Biao, Eisen , Michael B.

Identification of transcription factor binding sites (regulatory motifs) is a major interest in contemporary biology. We propose a new likelihood based method, COMODE, for identifying structural...

Supervised Detection of Regulatory Motifs in DNA Sequences (2003)

Keles, Sunduz, Van Der Laan, Mark J., Dudoit, Sandrine, Xing, Biao, Eisen , Michael B.

Identification of transcription factor binding sites (regulatory motifs) is a major interest in contemporary biology. We propose a new likelihood based method, COMODE, for identifying structural...

Phylogenetically and spatially conserved word pairs associated with gene-expression changes in yeasts (2003)

Chiang, Derek Y, Moses, Alan M, Kellis, Manolis, Lander, Eric S, Eisen, Michael B

Abstract Background Transcriptional regulation in eukaryotes often involves multiple transcription factors binding to the same transcription control region, and to understand the regulatory content...

Supervised Detection of Regulatory Motifs in DNA Sequences (2003)

Keles, Sunduz, Van Der Laan, Mark J., Dudoit, Sandrine, Xing, Biao, Eisen, Michael B.

Identification of transcription factor binding sites (regulatory motifs) is a major interest in contemporary biology. We propose a new likelihood based method, COMODE, for identifying structural...

Supervised Detection of Regulatory Motifs in DNA Sequences (2003)

Keles, Sunduz, Van Der Laan, Mark J., Dudoit, Sandrine, Xing, Biao, Eisen, Michael B.

Identification of transcription factor binding sites (regulatory motifs) is a major interest in contemporary biology. We propose a new likelihood based method, COMODE, for identifying structural...

Supervised Detection of Regulatory Motifs in DNA Sequences (2003)

Keles, Sunduz, Van Der Laan, Mark J., Dudoit, Sandrine, Xing, Biao, Eisen, Michael B.

Identification of transcription factor binding sites (regulatory motifs) is a major interest in contemporary biology. We propose a new likelihood based method, COMODE, for identifying structural...

Supervised Detection of Regulatory Motifs in DNA Sequences (2003)

Keles, Sunduz, Van Der Laan, Mark J., Dudoit, Sandrine, Xing, Biao, Eisen, Michael B.

Identification of transcription factor binding sites (regulatory motifs) is a major interest in contemporary biology. We propose a new likelihood based method, COMODE, for identifying structural...

Supervised detection of regulatory motifs in DNA sequences (2003)

Sunduz Keles, Rine Dudoit, Rine Dudoit, Biao Xing, Biao Xing, ...

Copyright c○2003 by the authors. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic,...

Supervised detection of regulatory motifs in DNA sequences (2003)

Rine Dudoit, Biao Xing, Michael B. Eisen

Identification of transcription factor binding sites (regulatory motifs) is a major interest in contemporary biology. We propose a new likelihood based method, COMODE, for identifying structural...

BMC Evolutionary Biology BioMed Central (2003)

Alan M Moses, Derek Y Chiang, Manolis Kellis, Eric S L, Michael B Eisen, Eric S Lander, ...

Research article Position specific variation in the rate of evolution in transcription factor binding sites

2003 Chiang et Volume al. 4, Issue 7, Article R43 Open Access (2003)

Derek Y Chiang, Alan M Moses, Manolis Kellis, Eric S L, Michael B Eisen

Phylogenetically and spatially conserved word pairs associated with gene-expression changes in yeasts

BMC Evolutionary Biology BioMed Central (2003)

Alan M Moses, Derek Y Chiang, Manolis Kellis, Eric S L, Michael B Eisen, Eric S Lander, ...

Research article Position specific variation in the rate of evolution in transcription factor binding sites

Exploring the conditional coregulation of yeast gene expression through fuzzy k-means clustering (2002)

Gasch, Audrey P, Eisen, Michael B

Abstract Background Organisms simplify the orchestration of gene expression by coregulating genes whose products function together in the cell. Many proteins serve different roles depending on the...

Identification of regulatory elements using a feature selection method (2002)

Keles, Sündüz, Van Der Laan, Mark, Eisen, Michael B.

Motivation: Many methods have been described to identify regulatory motifs in the transcription control regions of genes that exhibit similar patterns of gene expression across a variety of...

through fuzzy k-means (2002)

Audrey P Gasch, Michael B Eisen

the conditional coregulation of yeast gene expression

Identification of Regulatory Elements Using A Feature Selection Method (2001)

Keles, Sunduz, Van Der Laan, Mark J., Eisen, Michael B.

Many methods have been described to identify regulatory motifs in the transcription control regions of genes that exhibit similar patterns of gene expression across a variety of experimental...

Identification of Regulatory Elements Using A Feature Selection Method (2001)

Keles, Sunduz, Van Der Laan, Mark J., Eisen, Michael B.

Many methods have been described to identify regulatory motifs in the transcription control regions of genes that exhibit similar patterns of gene expression across a variety of experimental...

Identification of Regulatory Elements Using A Feature Selection Method (2001)

Keles, Sunduz, Van Der Laan, Mark J., Eisen, Michael B.

Many methods have been described to identify regulatory motifs in the transcription control regions of genes that exhibit similar patterns of gene expression across a variety of experimental...

Identification of Regulatory Elements Using A Feature Selection Method (2001)

Keles, Sunduz, Van Der Laan, Mark J., Eisen, Michael B.

Many methods have been described to identify regulatory motifs in the transcription control regions of genes that exhibit similar patterns of gene expression across a variety of experimental...

The Stanford Microarray Database (2001)

Sherlock, Gavin, Hernandez-Boussard, Tina, Kasarskis, Andrew, Binkley, Gail, Matese, John C., Dwight, Selina S., ...

The Stanford Microarray Database (SMD) stores raw and normalized data from microarray experiments, and provides web interfaces for researchers to retrieve, analyze and visualize their data. The two...

Visualizing associations between genome sequences and gene expression data using genome-mean expression profiles (2001)

Chiang, Derek Y., Brown, Patrick O., Eisen, Michael B.

The combination of genome-wide expression patterns and full genome sequences offers a great opportunity to further our understanding of the mechanisms and logic of transcriptional regulation. Many...

'Gene shaving' as a method for identifying distinct sets of genes with similar expression patterns (2000)

Hastie, Trevor, Tibshirani, Robert, Eisen, Michael B, Alizadeh, Ash, Levy, Ronald, Staudt, Louis, ...

Abstract Background Large gene expression studies, such as those conducted using DNA arrays, often provide millions of different pieces of data. To address the problem of analyzing such data, we...

Genomic expression programs in the response of yeast cells to environmental changes (2000)

Audrey P. Gasch, Paul T. Spellman, Camilla M. Kao, Orna Carmel-harel, Michael B. Eisen, Gisela Storz, ...

We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over...

Comment Review Reports Deposited Research Interactions Information Refereed (2000)

Research Gene Shaving', Trevor Hastie, Robert Tibshirani, Michael B Eisen, Ash Alizadeh, Ronald Levy, ...

Background: Large gene expression studies, such as those conducted using DNA arrays, often provide millions of different pieces of data. To address the problem of analyzing such data, we describe a...

ARTICLE NO. VY978526 Binding of the Influenza A Virus to Cell-Surface Receptors: Structures of Five Hemagglutinin– Sialyloligosaccharide Complexes Determined by X-Ray Crystallography (1996)

Michael B. Eisen, Subramaniam Sabesan, John J. Skehel, Don C. Wiley

The structures of five complexes of the X-31 influenza A (H3N2) virus hemagglutinin with sialyloligosaccharide receptor analogs have been determined from 2.5 to 2.8 A ˚ resolution by X-ray...

Genomic Expression Programs in the Response of Yeast Cells to Environmental ChangesD⃞

Gasch, Audrey P., Spellman, Paul T., Kao, Camilla M., Carmel-Harel, Orna, Eisen, Michael B., Storz, Gisela, ...

We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over...

Distinctive gene expression patterns in human mammary epithelial cells and breast cancers

Perou, Charles M., Jeffrey, Stefanie S., Van De Rijn, Matt, Rees, Christian A., Eisen, Michael B., Ross, Douglas T., ...

cDNA microarrays and a clustering algorithm were used to identify patterns of gene expression in human mammary epithelial cells growing in culture and in primary human breast tumors. Clusters of...

Identification of eukaryotic mRNAs that are translated at reduced cap binding complex eIF4F concentrations using a cDNA microarray

Johannes, Gregg, Carter, Mark S., Eisen, Michael B., Brown, Patrick O., Sarnow, Peter

Although most eukaryotic mRNAs need a functional cap binding complex eIF4F for efficient 5′ end- dependent scanning to initiate translation, picornaviral, hepatitis C viral, and a few cellular RNAs...

Cluster analysis and display of genome-wide expression patterns

Eisen, Michael B., Spellman, Paul T., Brown, Patrick O., Botstein, David

A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in...

Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray HybridizationD⃞

Spellman, Paul T., Sherlock, Gavin, Zhang, Michael Q., Iyer, Vishwanath R., Anders, Kirk, Eisen, Michael B., ...

We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures...

Ongoing immunoglobulin somatic mutation in germinal center B cell-like but not in activated B cell-like diffuse large cell lymphomas

Lossos, Izidore S., Alizadeh, Ash A., Eisen, Michael B., Chan, Wing C., Brown, Patrick O., Botstein, David, ...

B cell diffuse large cell lymphoma (B-DLCL) is a heterogeneous group of tumors, based on significant variations in morphology, clinical presentation, and response to treatment. Gene expression...

The Stanford Microarray Database

Sherlock, Gavin, Hernandez-Boussard, Tina, Kasarskis, Andrew, Binkley, Gail, Matese, John C., Dwight, Selina S., ...

The Stanford Microarray Database (SMD) stores raw and normalized data from microarray experiments, and provides web interfaces for researchers to retrieve, analyze and visualize their data. The two...

Delineating developmental and metabolic pathways in vivo by expression profiling using the RIKEN set of 18,816 full-length enriched mouse cDNA arrays

Miki, Rika, Kadota, Koji, Bono, Hidemasa, Mizuno, Yosuke, Tomaru, Yasuhiro, Carninci, Piero, ...

We have systematically characterized gene expression patterns in 49 adult and embryonic mouse tissues by using cDNA microarrays with 18,816 mouse cDNAs. Cluster analysis defined sets of genes that...

Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications

Sørlie, Therese, Perou, Charles M., Tibshirani, Robert, Aas, Turid, Geisler, Stephanie, Johnsen, Hilde, ...

The purpose of this study was to classify breast carcinomas based on variations in gene expression patterns derived from cDNA microarrays and to correlate tumor characteristics to clinical outcome. A...

Exploiting transcription factor binding site clustering to identify cis-regulatory modules involved in pattern formation in the Drosophila genome

Berman, Benjamin P., Nibu, Yutaka, Pfeiffer, Barret D., Tomancak, Pavel, Celniker, Susan E., Levine, Michael, ...

A major challenge in interpreting genome sequences is understanding how the genome encodes the information that specifies when and where a gene will be expressed. The first step in this process is...

Exploring the conditional coregulation of yeast gene expression through fuzzy k-means clustering

Gasch, Audrey P, Eisen, Michael B

A heuristically modified version of fuzzy k-means clustering has been used to identify overlapping clusters of yeast genes based on published gene-expression data following the response of yeast...

Phylogenetically and spatially conserved word pairs associated with gene-expression changes in yeasts

Chiang, Derek Y, Moses, Alan M, Kellis, Manolis, Lander, Eric S, Eisen, Michael B

The determination of conserved sequences has identified individual transcription factor binding sites. The incorporation of both joint conservation and spacing constraints of sequence pairs predicts...

Why PLoS Became a Publisher

Brown, Patrick O, Eisen, Michael B, Varmus, Harold E

Public Library of Science has grown from a grassroots movement to a nonprofit publisher, in order to catalyze change towards open-access publishing of the scientific literature

Population genetic variation in gene expression is associated with phenotypic variation in Saccharomyces cerevisiae

Fay, Justin C, McCullough, Heather L, Sniegowski, Paul D, Eisen, Michael B

This study compared levels of gene expression among nine natural isolates of Saccharomyces cerevisiae grown in the presence or absence of copper sulfate. Insights were gained into the molecular basis...

Noise Minimization in Eukaryotic Gene Expression

Fraser, Hunter B, Hirsh, Aaron E, Giaever, Guri, Kumm, Jochen, Eisen, Michael B

All organisms have elaborate mechanisms to control rates of protein production. However, protein production is also subject to stochastic fluctuations, or “noise.” Several recent studies in...

Coevolution of gene expression among interacting proteins

Fraser, Hunter B., Hirsh, Aaron E., Wall, Dennis P., Eisen, Michael B.

Physically interacting proteins or parts of proteins are expected to evolve in a coordinated manner that preserves proper interactions. Such coevolution at the amino acid-sequence level is well...

Detecting DNA regulatory motifs by incorporating positional trends in information content

Kechris, Katherina J, Van Zwet, Erik, Bickel, Peter J, Eisen, Michael B

On the basis of the observation that conserved positions in transcription factor binding sites are often clustered together, a simple extension to the model-based motif discovery method is proposed....

Computational identification of developmental enhancers: conservation and function of transcription factor binding-site clusters in Drosophila melanogaster and Drosophila pseudoobscura

Berman, Benjamin P, Pfeiffer, Barret D, Laverty, Todd R, Salzberg, Steven L, Rubin, Gerald M, Eisen, Michael B, ...

27 predicted gene-regulatory regions in the Drosophila melanogaster genome were analyzed in vivo, confirming 15 active enhancer regions. A comparison with Drosophila pseudoobscura sequences revealed...

Conservation and Evolution of Cis-Regulatory Systems in Ascomycete Fungi

Gasch, Audrey P, Moses, Alan M, Chiang, Derek Y, Fraser, Hunter B, Berardini, Mark, Eisen, Michael B

Relatively little is known about the mechanisms through which gene expression regulation evolves. To investigate this, we systematically explored the conservation of regulatory networks in fungi by...

Serendipitous discovery of Wolbachia genomes in multiple Drosophila species

Salzberg, Steven L, Hotopp, Julie C Dunning, Delcher, Arthur L, Pop, Mihai, Smith, Douglas R, Eisen, Michael B, ...

By searching the publicly available repository of DNA sequencing trace data, we discovered three new species of the bacterial endosymbiont Wolbachia pipientis in three different species of fruit fly:...

Correction: Serendipitous discovery of Wolbachia genomes in multiple Drosophila species

Salzberg, Steven L, Dunning Hotopp, Julie C, Delcher, Arthur L, Pop, Mihai, Smith, Douglas R, Eisen, Michael B, ...

A correction to Serendipitous discovery of Wolbachia genomes in multiple Drosophila species by SL Salzberg, JC Dunning Hotopp, AL Delcher, M Pop, DR Smith, MB Eisen and WC Nelson. Genome Biology...

Aging and Gene Expression in the Primate Brain

Fraser, Hunter B, Khaitovich, Philipp, Plotkin, Joshua B, Pääbo, Svante, Eisen, Michael B

It is well established that gene expression levels in many organisms change during the aging process, and the advent of DNA microarrays has allowed genome-wide patterns of transcriptional changes...

Rapid quantitative profiling of complex microbial populations

Palmer, Chana, Bik, Elisabeth M., Eisen, Michael B., Eckburg, Paul B., Sana, Theodore R., Wolber, Paul K., ...

Diverse and complex microbial ecosystems are found in virtually every environment on earth, yet we know very little about their composition and ecology. Comprehensive identification and...

Identification of oligonucleotide sequences that direct the movement of the Escherichia coli FtsK translocase

Levy, Oren, Ptacin, Jerod L., Pease, Paul J., Gore, Jeff, Eisen, Michael B., Bustamante, Carlos, ...

FtsK from Escherichia coli is a fast and sequence-directed DNA translocase with roles in chromosome dimer resolution, segregation, and decatenation. From the movement of single FtsK particles on...

Large-Scale Turnover of Functional Transcription Factor Binding Sites in Drosophila

Moses, Alan M, Pollard, Daniel A, Nix, David A, Iyer, Venky N, Li, Xiao-Yong, Biggin, Mark D, ...

The gain and loss of functional transcription factor binding sites has been proposed as a major source of evolutionary change in cis-regulatory DNA and gene expression. We have developed an...

Widespread Discordance of Gene Trees with Species Tree in Drosophila: Evidence for Incomplete Lineage Sorting

Pollard, Daniel A, Iyer, Venky N, Moses, Alan M, Eisen, Michael B

The phylogenetic relationship of the now fully sequenced species Drosophila erecta and D. yakuba with respect to the D. melanogaster species complex has been a subject of controversy. All three...

Genomic Expression Programs in the Response of Yeast Cells to Environmental ChangesD⃞

Gasch, Audrey P., Spellman, Paul T., Kao, Camilla M., Carmel-Harel, Orna, Eisen, Michael B., Storz, Gisela, ...

We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over...

Distinctive gene expression patterns in human mammary epithelial cells and breast cancers

Perou, Charles M., Jeffrey, Stefanie S., Van De Rijn, Matt, Rees, Christian A., Eisen, Michael B., Ross, Douglas T., ...

cDNA microarrays and a clustering algorithm were used to identify patterns of gene expression in human mammary epithelial cells growing in culture and in primary human breast tumors. Clusters of...

Identification of eukaryotic mRNAs that are translated at reduced cap binding complex eIF4F concentrations using a cDNA microarray

Johannes, Gregg, Carter, Mark S., Eisen, Michael B., Brown, Patrick O., Sarnow, Peter

Although most eukaryotic mRNAs need a functional cap binding complex eIF4F for efficient 5′ end- dependent scanning to initiate translation, picornaviral, hepatitis C viral, and a few cellular RNAs...

Cluster analysis and display of genome-wide expression patterns

Eisen, Michael B., Spellman, Paul T., Brown, Patrick O., Botstein, David

A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in...

Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray HybridizationD⃞

Spellman, Paul T., Sherlock, Gavin, Zhang, Michael Q., Iyer, Vishwanath R., Anders, Kirk, Eisen, Michael B., ...

We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures...

Ongoing immunoglobulin somatic mutation in germinal center B cell-like but not in activated B cell-like diffuse large cell lymphomas

Lossos, Izidore S., Alizadeh, Ash A., Eisen, Michael B., Chan, Wing C., Brown, Patrick O., Botstein, David, ...

B cell diffuse large cell lymphoma (B-DLCL) is a heterogeneous group of tumors, based on significant variations in morphology, clinical presentation, and response to treatment. Gene expression...

The Stanford Microarray Database

Sherlock, Gavin, Hernandez-Boussard, Tina, Kasarskis, Andrew, Binkley, Gail, Matese, John C., Dwight, Selina S., ...

The Stanford Microarray Database (SMD) stores raw and normalized data from microarray experiments, and provides web interfaces for researchers to retrieve, analyze and visualize their data. The two...

Delineating developmental and metabolic pathways in vivo by expression profiling using the RIKEN set of 18,816 full-length enriched mouse cDNA arrays

Miki, Rika, Kadota, Koji, Bono, Hidemasa, Mizuno, Yosuke, Tomaru, Yasuhiro, Carninci, Piero, ...

We have systematically characterized gene expression patterns in 49 adult and embryonic mouse tissues by using cDNA microarrays with 18,816 mouse cDNAs. Cluster analysis defined sets of genes that...

Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications

Sørlie, Therese, Perou, Charles M., Tibshirani, Robert, Aas, Turid, Geisler, Stephanie, Johnsen, Hilde, ...

The purpose of this study was to classify breast carcinomas based on variations in gene expression patterns derived from cDNA microarrays and to correlate tumor characteristics to clinical outcome. A...

Exploiting transcription factor binding site clustering to identify cis-regulatory modules involved in pattern formation in the Drosophila genome

Berman, Benjamin P., Nibu, Yutaka, Pfeiffer, Barret D., Tomancak, Pavel, Celniker, Susan E., Levine, Michael, ...

A major challenge in interpreting genome sequences is understanding how the genome encodes the information that specifies when and where a gene will be expressed. The first step in this process is...

Exploring the conditional coregulation of yeast gene expression through fuzzy k-means clustering

Gasch, Audrey P, Eisen, Michael B

A heuristically modified version of fuzzy k-means clustering has been used to identify overlapping clusters of yeast genes based on published gene-expression data following the response of yeast...

Phylogenetically and spatially conserved word pairs associated with gene-expression changes in yeasts

Chiang, Derek Y, Moses, Alan M, Kellis, Manolis, Lander, Eric S, Eisen, Michael B

The determination of conserved sequences has identified individual transcription factor binding sites. The incorporation of both joint conservation and spacing constraints of sequence pairs predicts...

Why PLoS Became a Publisher

Brown, Patrick O, Eisen, Michael B, Varmus, Harold E

Public Library of Science has grown from a grassroots movement to a nonprofit publisher, in order to catalyze change towards open-access publishing of the scientific literature

Population genetic variation in gene expression is associated with phenotypic variation in Saccharomyces cerevisiae

Fay, Justin C, McCullough, Heather L, Sniegowski, Paul D, Eisen, Michael B

This study compared levels of gene expression among nine natural isolates of Saccharomyces cerevisiae grown in the presence or absence of copper sulfate. Insights were gained into the molecular basis...

Noise Minimization in Eukaryotic Gene Expression

Fraser, Hunter B, Hirsh, Aaron E, Giaever, Guri, Kumm, Jochen, Eisen, Michael B

All organisms have elaborate mechanisms to control rates of protein production. However, protein production is also subject to stochastic fluctuations, or “noise.” Several recent studies in...

Coevolution of gene expression among interacting proteins

Fraser, Hunter B., Hirsh, Aaron E., Wall, Dennis P., Eisen, Michael B.

Physically interacting proteins or parts of proteins are expected to evolve in a coordinated manner that preserves proper interactions. Such coevolution at the amino acid-sequence level is well...

Detecting DNA regulatory motifs by incorporating positional trends in information content

Kechris, Katherina J, Van Zwet, Erik, Bickel, Peter J, Eisen, Michael B

On the basis of the observation that conserved positions in transcription factor binding sites are often clustered together, a simple extension to the model-based motif discovery method is proposed....

Computational identification of developmental enhancers: conservation and function of transcription factor binding-site clusters in Drosophila melanogaster and Drosophila pseudoobscura

Berman, Benjamin P, Pfeiffer, Barret D, Laverty, Todd R, Salzberg, Steven L, Rubin, Gerald M, Eisen, Michael B, ...

27 predicted gene-regulatory regions in the Drosophila melanogaster genome were analyzed in vivo, confirming 15 active enhancer regions. A comparison with Drosophila pseudoobscura sequences revealed...

Conservation and Evolution of Cis-Regulatory Systems in Ascomycete Fungi

Gasch, Audrey P, Moses, Alan M, Chiang, Derek Y, Fraser, Hunter B, Berardini, Mark, Eisen, Michael B

Relatively little is known about the mechanisms through which gene expression regulation evolves. To investigate this, we systematically explored the conservation of regulatory networks in fungi by...

Functional genomic analysis of the rates of protein evolution

Wall, Dennis P., Hirsh, Aaron E., Fraser, Hunter B., Kumm, Jochen, Giaever, Guri, Eisen, Michael B., ...

The evolutionary rates of proteins vary over several orders of magnitude. Recent work suggests that analysis of large data sets of evolutionary rates in conjunction with the results from...

Serendipitous discovery of Wolbachia genomes in multiple Drosophila species

Salzberg, Steven L, Hotopp, Julie C Dunning, Delcher, Arthur L, Pop, Mihai, Smith, Douglas R, Eisen, Michael B, ...

By searching the publicly available repository of DNA sequencing trace data, we discovered three new species of the bacterial endosymbiont Wolbachia pipientis in three different species of fruit fly:...

Correction: Serendipitous discovery of Wolbachia genomes in multiple Drosophila species

Salzberg, Steven L, Dunning Hotopp, Julie C, Delcher, Arthur L, Pop, Mihai, Smith, Douglas R, Eisen, Michael B, ...

A correction to Serendipitous discovery of Wolbachia genomes in multiple Drosophila species by SL Salzberg, JC Dunning Hotopp, AL Delcher, M Pop, DR Smith, MB Eisen and WC Nelson. Genome Biology...

Aging and Gene Expression in the Primate Brain

Fraser, Hunter B, Khaitovich, Philipp, Plotkin, Joshua B, Pääbo, Svante, Eisen, Michael B

It is well established that gene expression levels in many organisms change during the aging process, and the advent of DNA microarrays has allowed genome-wide patterns of transcriptional changes...

Identification of oligonucleotide sequences that direct the movement of the Escherichia coli FtsK translocase

Levy, Oren, Ptacin, Jerod L., Pease, Paul J., Gore, Jeff, Eisen, Michael B., Bustamante, Carlos, ...

FtsK from Escherichia coli is a fast and sequence-directed DNA translocase with roles in chromosome dimer resolution, segregation, and decatenation. From the movement of single FtsK particles on...

Rapid quantitative profiling of complex microbial populations

Palmer, Chana, Bik, Elisabeth M., Eisen, Michael B., Eckburg, Paul B., Sana, Theodore R., Wolber, Paul K., ...

Diverse and complex microbial ecosystems are found in virtually every environment on earth, yet we know very little about their composition and ecology. Comprehensive identification and...

Large-Scale Turnover of Functional Transcription Factor Binding Sites in Drosophila

Moses, Alan M, Pollard, Daniel A, Nix, David A, Iyer, Venky N, Li, Xiao-Yong, Biggin, Mark D, ...

The gain and loss of functional transcription factor binding sites has been proposed as a major source of evolutionary change in cis-regulatory DNA and gene expression. We have developed an...

Widespread Discordance of Gene Trees with Species Tree in Drosophila: Evidence for Incomplete Lineage Sorting

Pollard, Daniel A, Iyer, Venky N, Moses, Alan M, Eisen, Michael B

The phylogenetic relationship of the now fully sequenced species Drosophila erecta and D. yakuba with respect to the D. melanogaster species complex has been a subject of controversy. All three...

Primate-specific evolution of an LDLR enhancer

Wang, Qian-fei, Prabhakar, Shyam, Wang, Qianben, Moses, Alan M, Chanan, Sumita, Brown, Myles, ...

Analysis of primate-specific evolution of the LDL receptor enhancer demonstrates a molecular mechanism by which ancestral mammalian regulatory elements can evolve to perform new functions.

Three-dimensional morphology and gene expression in the Drosophila blastoderm at cellular resolution I: data acquisition pipeline

Luengo Hendriks, Cris L, Keränen, Soile VE, Fowlkes, Charless C, Simirenko, Lisa, Weber, Gunther H, DePace, Angela H, ...

A suite of methods that provide the first quantitative three-dimensional description of gene expression and morphology with cellular resolution in whole Drosophila embryos is described.

Determining Physical Constraints in Transcriptional Initiation Complexes Using DNA Sequence Analysis

Shultzaberger, Ryan K., Chiang, Derek Y., Moses, Alan M., Eisen, Michael B.

Eukaryotic gene expression is often under the control of cooperatively acting transcription factors whose binding is limited by structural constraints. By determining these structural constraints, we...

Transcription Factors Bind Thousands of Active and Inactive Regions in the Drosophila Blastoderm

Li, Xiao-yong, MacArthur, Stewart, Bourgon, Richard, Nix, David, Pollard, Daniel A, Iyer, Venky N, ...

Identifying the genomic regions bound by sequence-specific regulatory factors is central both to deciphering the complex DNA cis-regulatory code that controls transcription in metazoans and to...

Sepsid even-skipped Enhancers Are Functionally Conserved in Drosophila Despite Lack of Sequence Conservation

Hare, Emily E., Peterson, Brant K., Iyer, Venky N., Meier, Rudolf, Eisen, Michael B.

The gene expression pattern specified by an animal regulatory sequence is generally viewed as arising from the particular arrangement of transcription factor binding sites it contains. However, we...

Silent but Not Static: Accelerated Base-Pair Substitution in Silenced Chromatin of Budding Yeasts

Teytelman, Leonid, Eisen, Michael B., Rine, Jasper

Subtelomeric DNA in budding yeasts, like metazoan heterochromatin, is gene poor, repetitive, transiently silenced, and highly dynamic. The rapid evolution of subtelomeric regions is commonly thought...

Tools for neuroanatomy and neurogenetics in Drosophila

Pfeiffer, Barret D., Jenett, Arnim, Hammonds, Ann S., Ngo, Teri-T B., Misra, Sima, Murphy, Christine, ...

We demonstrate the feasibility of generating thousands of transgenic Drosophila melanogaster lines in which the expression of an exogenous gene is reproducibly directed to distinct small subsets of...

Big Genomes Facilitate the Comparative Identification of Regulatory Elements

Peterson, Brant K., Hare, Emily E., Iyer, Venky N., Storage, Steven, Conner, Laura, Papaj, Daniel R., ...

The identification of regulatory sequences in animal genomes remains a significant challenge. Comparative genomic methods that use patterns of evolutionary conservation to identify non-coding...

Global gene expression profiles for life stages of the deadly amphibian pathogen Batrachochytrium dendrobatidis

Rosenblum, Erica Bree, Stajich, Jason E., Maddox, Nicole, Eisen, Michael B.

Amphibians around the world are being threatened by an emerging pathogen, the chytrid fungus Batrachochytrium dendrobatidis (Bd). Despite intensive ecological study in the decade since Bd was...

Developmental roles of 21 Drosophila transcription factors are determined by quantitative differences in binding to an overlapping set of thousands of genomic regions

MacArthur, Stewart, Li, Xiao-Yong, Li, Jingyi, Brown, James B, Chu, Hou Cheng, Zeng, Lucy, ...

Distinct developmental fates in Drosophila melanogaster are specified by quantitative differences in transcription factor occupancy on a common set of bound regions.

Impact of Chromatin Structures on DNA Processing for Genomic Analyses

Teytelman, Leonid, Özaydın, Bilge, Zill, Oliver, Lefrançois, Philippe, Snyder, Michael, Rine, Jasper, ...

Chromatin has an impact on recombination, repair, replication, and evolution of DNA. Here we report that chromatin structure also affects laboratory DNA manipulation in ways that distort the results...

Genome-Wide Transcriptional Response of Silurana (Xenopus) tropicalis to Infection with the Deadly Chytrid Fungus

Rosenblum, Erica Bree, Poorten, Thomas J., Settles, Matthew, Murdoch, Gordon K., Robert, Jacques, Maddox, Nicole, ...

Emerging infectious diseases are of great concern for both wildlife and humans. Several highly virulent fungal pathogens have recently been discovered in natural populations, highlighting the need...

A condensin-like dosage compensation complex acts at a distance to control expression throughout the genome

Jans, Judith, Gladden, John M., Ralston, Edward J., Pickle, Catherine S., Michel, Agnès H., Pferdehirt, Rebecca R., ...

In many species, a dosage compensation complex (DCC) is targeted to X chromosomes of one sex to equalize levels of X-gene products between males (1X) and females (2X). Here we identify cis-acting...