Integrative modeling of transcriptional regulation in response to antirheumatic therapy (2009)
Hecker, Michael, Goertsches, Robert, Engelmann, Robby, Thiesen, Hans-Juergen, Guthke, Reinhard
Abstract Background The investigation of gene regulatory networks is an important issue in molecular systems biology and significant progress has been made by combining different types of biological...
Guidelines for the next 10 years of proteomics (2009)
Wilkins, Marc R, Appel, Ron D, Van Eyk, Jennifer E, Chung, Maxey C M, Görg, Angelika, Hecker, Michael, ...
In the last ten years, the field of proteomics has expanded at a rapid rate. A range of exciting new technology has been developed and enthusiastically applied to an enormous variety of biological...
A generic privacy ontology and its applications to different domains (2009)
Privacy is becoming increasingly important due to the advent of e-commerce, but is equally important in other application domains. Domain applications frequently require customers to divulge many...
Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, (2008)
Helga Westers, Ronald Dorenbos, Jorrit Kabel, Tony Flanagan, Kevin M. Devine, ...
Reconstructing the Bacillus genome (Westers et al.) Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is...
Koczan, Dirk, Drynda, Susanne, Hecker, Michael, Drynda, Andreas, Guthke, Reinhard, Kekow, Joern, ...
Abstract Introduction About 30% of rheumatoid arthritis patients fail to respond adequately to TNFα-blocking therapy. There is a medical and socioeconomic need to identify molecular markers for an...
Zweers, Jessica C, Barák, Imrich, Becher, Dörte, Driessen, Arnold JM, Hecker, Michael, Kontinen, Vesa P, ...
Abstract Background The Gram-positive bacterium Bacillus subtilis is an important producer of high quality industrial enzymes and a few eukaryotic proteins. Most of these proteins are secreted into...
Privacy ontology support for E-commerce (2008)
Hecker, Michael, Dillon, Tharam S, Chang, Elizabeth
Privacy is becoming increasingly important due to the advent of e-commerce. E-commerce applications frequently require customers to divulge many personal details about themselves that must be...
Zweers, Jessica C., Barák, Imrich, Becher, Dörte, Driessen, Arnold J.M., Hecker, Michael, Kontinen, Vesa P., ...
Background: The Gram-positive bacterium Bacillus subtilis is an important producer of high quality industrial enzymes and a few eukaryotic proteins. Most of these proteins are secreted into the...
Privacy ontology support for E-commerce (2008)
Hecker, Michael, Dillon, Tharam S, Chang, Elizabeth
Privacy is becoming increasingly important due to the advent of e-commerce. E-commerce applications frequently require customers to divulge many personal details about themselves that must be...
Privacy ontology support for E-commerce (2008)
Hecker, Michael, Dillon, Tharam S., Chang, Elizabeth
Privacy is becoming increasingly important due to the advent of e-commerce. E-commerce applications frequently require customers to divulge many personal details about themselves that must be...
Privacy ontology support for E-commerce (2008)
Hecker, Michael, Dillon, Tharam S., Chang, Elizabeth
Privacy is becoming increasingly important due to the advent of e-commerce. E-commerce applications frequently require customers to divulge many personal details about themselves that must be...
Integrated network reconstruction, visualization and analysis using YANAsquare (2007)
Schwarz, Roland, Liang, Chunguang, Kaleta, Christoph, Kühnel, Mark, Hoffmann, Eik, Kuznetsov, Sergei, ...
Abstract Background Modeling of metabolic networks includes tasks such as network assembly, network overview, calculation of metabolic fluxes and testing the robustness of the network. Results...
Das Thema Technische Dokumentation (TD) gewinnt auf Grund von verschärften Rechtsvorschriften einerseits und gestiegenen Anforderungen von Seiten der Kunden andererseits an Bedeutung für die...
Mit der Strömung des Strukturalismus in Architektur und Städtebau, innerhalb des Untersuchungszeitraumes, verband sich eine vehement geführte Kulturkritik an einem sich abzeichnenden...
Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila (2007)
Markert, Stephanie, Arndt, Cordelia, Felbeck, Horst, Becher, Dörte, Sievert, Stefan M., Hügler, Michael, ...
Duisburg, Essen, Univ., Diss., 2007
Chunguang Liang, Christoph Kaleta, Mark Kühnel, Eik Hoffmann, Sergei Kuznetsov, Michael Hecker, ...
© 2007 Schwarz et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License
Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila (2006)
Markert, Stephanie, Arndt, Cordelia, Felbeck, Horst, Becher, Dorte, Sievert, Stefan M., Hugler, Michael, ...
Author Posting. © The Authors, 2006. This is the author's version of the work. It is posted here by permission of AAAS for personal use, not for redistribution. The definitive version was published...
Monitoring of stress responses (2006)
Schweder, Thomas, Jürgen, Britta, Voigt, Birgit, Pioch, Daniel, Hecker, Michael, Evers, Stefan, ...
No abstract available.
Darmon, Elise, Dorenbos, Ronald, Meens, Jochen, Freudl, Roland, Antelmann, Haike, Hecker, Michael, ...
The gram-positive bacterium Bacillus subtilis secretes high levels of proteins into its environment. Most of these secretory proteins are exported from the cytoplasm in an unfolded state and have to...
Proteomics of Microbial Pathogens (2006)
Jungblut, Peter R., Hecker, Michael
Book Description Containing proven, high-quality research articles selected from the popular Proteomics journal, this is a current overview of the latest research into the proteomics analysis of...
Guidelines for the next 10 years of proteomics. (2006)
Wilkins, Marc R, Appel, Ron D, Van Eyk, Jennifer E, Chung, Maxey C M, Görg, Angelika, Hecker, Michael, ...
In the last ten years, the field of proteomics has expanded at a rapid rate. A range of exciting new technology has been developed and enthusiastically applied to an enormous variety of biological...
Guidelines for the next 10 years of proteomics. (2006)
Wilkins, Marc R, Appel, Ron D, Van Eyk, Jennifer E, Chung, Maxey C M, Görg, Angelika, Hecker, Michael, ...
In the last ten years, the field of proteomics has expanded at a rapid rate. A range of exciting new technology has been developed and enthusiastically applied to an enormous variety of biological...
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
The extracellular proteome of Bacillus subtilis under secretion stress conditions (2003)
Antelmann, Haike, Darmon, Elise, Noone, David, Veening, Jan-Willem, Westers, Helga, Bron, Sierd, ...
The accumulation of malfolded proteins in the cell envelope of the Gram-positive eubacterium Bacillus subtilis was previously shown to provoke a so-called secretion stress response. In the present...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Dijl, Jan Maarten Van, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes, as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining nonessential genes of an organism,...
Bernhardt, Jörg, Weibezahn, Jimena, Scharf, Christian, Hecker, Michael
Dual channel imaging and warping of two-dimensional (2D) protein gels were used to visualize global changes of the gene expression patterns in growing Bacillus subtilis cells during entry into the...
Genome Engineering Reveals Large Dispensable Regions in Bacillus subtilis (2003)
Westers, Helga, Dorenbos, Ronald, Van Dijl, Jan Maarten, Kabel, Jorrit, Flanagan, Tony, Devine, Kevin M., ...
Bacterial genomes contain 250 to 500 essential genes as suggested by single gene disruptions and theoretical considerations. If this view is correct, the remaining non-essential genes of an organism,...
Magnetostriction and Microstructure of As-Deposited and Annealed Co Thin Films (2002)
Brueckner, Winfried, Hecker, Michael, Thomas, Juergen, Tietjen, Detlev, Schneider, Claus M.
The magnetostriction of as-sputtered and annealed 400 nm thick Co films has been studied in longitudinal and transverse magnetic fields. The appreciable change of the magnetostriction behavior after...
Dresden, Techn. Universiẗat, Diss., 1993.
Hecker, Michael., Müller, Hartmut.
Greifswald, Univ., Math.-Naturwiss. Fak., Diss. A, 1973. (Nicht f. d. Austausch.).
Völker, Uwe, Maul, Björn, Hecker, Michael
The alternative sigma factor ςB of Bacillus subtilis is required for the induction of approximately 100 genes after the imposition of a whole range of stresses and energy limitation. In this study,...
Tobisch, Steffen, Stülke, Jörg, Hecker, Michael
The lic operon of Bacillus subtilis is required for the transport and degradation of oligomeric β-glucosides, which are produced by extracellular enzymes on substrates such as lichenan or barley...
Petersohn, Anja, Bernhardt, Jörg, Gerth, Ulf, Höper, Dirk, Koburger, Torsten, Völker, Uwe, ...
A consensus-directed search for ςB promoters was used to locate potential candidates for new ςB-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations...
Role of CcpA in Regulation of the Central Pathways of Carbon Catabolism in Bacillus subtilis
Tobisch, Steffen, Zühlke, Daniela, Bernhardt, Jörg, Stülke, Jörg, Hecker, Michael
The Bacillus subtilis two-dimensional (2D) protein index contains almost all glycolytic and tricarboxylic acid (TCA) cycle enzymes, among them the most abundant housekeeping proteins of growing...
The Clp Proteases of Bacillus subtilis Are Directly Involved in Degradation of Misfolded Proteins
Krüger, Elke, Witt, Elke, Ohlmeier, Steffen, Hanschke, Renate, Hecker, Michael
The presence of the heat stress response-related ATPases ClpC and ClpX or the peptidase ClpP in the cell is crucial for tolerance of many forms of stress in Bacillus subtilis. Assays for detection of...
Antelmann, Haike, Scharf, Christian, Hecker, Michael
The phosphate starvation response in Bacillus subtilis was analyzed using two-dimensional (2D) polyacrylamide gel electrophoresis of cell extracts and supernatants from phosphate-starved cells. Most...
Characterization of the ςB Regulon in Staphylococcus aureus
Gertz, Silke, Engelmann, Susanne, Schmid, Roland, Ziebandt, Anne-Kathrin, Tischer, Karsten, Scharf, Christian, ...
The ςB-dependent stress regulon in gram-positive bacteria might fulfill a physiological role in stress response and virulence similar to that of the ςS regulon in Escherichia coli and other...
Putative Sigma Factor SigI (YkoZ) of Bacillus subtilis Is Induced by Heat Shock
Zuber, Ulrich, Drzewiecki, Kathrin, Hecker, Michael
A Bacillus subtilis disruption mutant with a mutation in sigI (formerly ykoZ) shows a temperature-sensitive growth on agar plates. The transcription of the sigI gene is heat shock induced in rich...
Global Analysis of the General Stress Response of Bacillus subtilis
Petersohn, Anja, Brigulla, Matthias, Haas, Stefan, Hoheisel, Jörg D., Völker, Uwe, Hecker, Michael
Gene arrays containing all currently known open reading frames of Bacillus subtilis were used to examine the general stress response of Bacillus. By proteomics, transcriptional analysis, transposon...
Thioredoxin Is an Essential Protein Induced by Multiple Stresses in Bacillus subtilis
Scharf, Christian, Riethdorf, Sabine, Ernst, Henrik, Engelmann, Susanne, Völker, Uwe, Hecker, Michael
Thioredoxin, a small, ubiquitous protein which participates in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, is an essential protein in Bacillus...
Nonnative Proteins Induce Expression of the Bacillus subtilis CIRCE Regulon
Mogk, Axel, Völker, Andrea, Engelmann, Susanne, Hecker, Michael, Schumann, Wolfgang, Völker, Uwe
The chaperone-encoding groESL and dnaK operons constitute the CIRCE regulon of Bacillus subtilis. Both operons are under negative control of the repressor protein HrcA, which interacts with the CIRCE...
One of Two OsmC Homologs in Bacillus subtilis Is Part of the ςB-Dependent General Stress Regulon
Völker, Uwe, Andersen, Kasper Krogh, Antelmann, Haike, Devine, Kevin M., Hecker, Michael
In this report we present the identification and analysis of two Bacillus subtilis genes, yklA and ykzA, which are homologous to the partially RpoS-controlled osmC gene from Escherichia coli. The...
The yvyD Gene of Bacillus subtilis Is under Dual Control of ςB and ςH
Drzewiecki, Kathrin, Eymann, Christine, Mittenhuber, Gerhard, Hecker, Michael
During a search by computer-aided inspection of two-dimensional (2D) protein gels for ςB-dependent general stress proteins exhibiting atypical induction profiles, a protein initially called Hst23...
The Bacillus subtilis clpC operon is regulated by two stress induction pathways relying on either ςB or a class III stress induction mechanism acting at a ςA-like promoter. When the clpC operon was...
Rachid, Shwan, Ohlsen, Knut, Wallner, Ursula, Hacker, Jörg, Hecker, Michael, Ziebuhr, Wilma
Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of a global regulator of the bacterial stress response, the alternative transcription...
Pummi, Tiina, Leskelä, Soile, Wahlström, Eva, Gerth, Ulf, Tjalsma, Harold, Hecker, Michael, ...
Identification and characterization of a suppressor mutation, sup-15, which partially restored secretion in the protein secretion-deficient Bacillus subtilis ecsA26 mutant, led us to discover a novel...
Heat Shock Proteome of Agrobacterium tumefaciens: Evidence for New Control Systems
Rosen, Ran, Büttner, Knut, Becher, Dörte, Nakahigashi, Kenji, Yura, Takashi, Hecker, Michael, ...
The regulation of Agrobacterium tumefaciens heat shock genes involves a transcriptional activator (RpoH) and repressor elements (HrcA-CIRCE). Using proteome analysis and mutants in these control...
Eymann, Christine, Homuth, Georg, Scharf, Christian, Hecker, Michael
The stringent response in Bacillus subtilis was characterized by using proteome and transcriptome approaches. Comparison of protein synthesis patterns of wild-type and relA mutant cells cultivated...
Mäder, Ulrike, Homuth, Georg, Scharf, Christian, Büttner, Knut, Bode, Rüdiger, Hecker, Michael
A comprehensive study of Bacillus subtilis gene expression patterns in response to amino acid availability was performed by means of proteomics and transcriptomics. The methods of two-dimensional...
Lindner, Cordula, Hecker, Michael, Le Coq, Dominique, Deutscher, Josef
The Bacillus subtilis antiterminator LicT regulates the expression of bglPH and bglS, which encode the enzymes for the metabolism of aryl-β-glucosides and the β-glucanase BglS. The N-terminal...
Bandow, Julia Elisabeth, Brötz, Heike, Hecker, Michael
Low concentrations of the RNA polymerase inhibitor rifampin added to an exponentially growing culture of Bacillus subtilis led to an instant inhibition of growth. Survival experiments revealed that...
Binding of σA and σB to Core RNA Polymerase after Environmental Stress in Bacillus subtilis
Rollenhagen, Claudia, Antelmann, Haike, Kirstein, Janine, Delumeau, Olivier, Hecker, Michael, Yudkin, Michael D.
In Bacillus subtilis, the alternative sigma factor σB is activated in response to environmental stress or energy depletion. The general stress regulon under the control of σB provides the cell with...
Clp-mediated proteolysis in Gram-positive bacteria is autoregulated by the stability of a repressor
Krüger, Elke, Zühlke, Daniela, Witt, Elke, Ludwig, Holger, Hecker, Michael
The heat shock proteins ClpC and ClpP are subunits of an ATP-dependent protease of Bacillus subtilis. Under non-stressed conditions, transcription of the clpC and clpP genes is negatively regulated...
Proteomic Approach to Understanding Antibiotic Action
Bandow, Julia Elisabeth, Brötz, Heike, Leichert, Lars Ingo Ole, Labischinski, Harald, Hecker, Michael
We have used proteomic technology to elucidate the complex cellular responses of Bacillus subtilis to antimicrobial compounds belonging to classical and emerging antibiotic classes. We established on...
Global Characterization of Disulfide Stress in Bacillus subtilis
Leichert, Lars Ingo Ole, Scharf, Christian, Hecker, Michael
We used DNA macroarray and proteome analysis to analyze the regulatory networks in Bacillus subtilis that are affected by disulfide stress. To induce disulfide stress, we used the specific thiol...
Kohler, Christian, Von Eiff, Christof, Peters, Georg, Proctor, Richard A., Hecker, Michael, Engelmann, Susanne
The high-resolution two-dimensional (2D) protein gel electrophoresis technique combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry was used for identification...
Fine-Tuning in Regulation of Clp Protein Content in Bacillus subtilis†
Gerth, Ulf, Kirstein, Janine, Mostertz, Jörg, Waldminghaus, Torsten, Miethke, Marcus, Kock, Holger, ...
Clp-controlled proteolysis in Bacillus subtilis seems to play a substantial role, particularly under stress conditions. Calibrated Western blot analyses were used to estimate the approximate numbers...
Mäder, Ulrike, Hennig, Susanne, Hecker, Michael, Homuth, Georg
In Bacillus subtilis, the genes of the branched-chain amino acids biosynthetic pathway are organized in three genetic loci: the ilvBHC-leuABCD (ilv-leu) operon, ilvA, and ilvD. These genes, as well...
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
Secretory proteins perform a variety of important “remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make...
Bernhardt, Jörg, Weibezahn, Jimena, Scharf, Christian, Hecker, Michael
Dual channel imaging and warping of two-dimensional (2D) protein gels were used to visualize global changes of the gene expression patterns in growing Bacillus subtilis cells during entry into the...
Zanen, Geeske, Antelmann, Haike, Westers, Helga, Hecker, Michael, Van Dijl, Jan Maarten, Quax, Wim J.
Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and...
The ClpP Peptidase Is the Major Determinant of Bulk Protein Turnover in Bacillus subtilis
Kock, Holger, Gerth, Ulf, Hecker, Michael
Measurements of overall protein degradation rates in wild-type and clpP mutant Bacillus subtilis cells revealed that stress- or starvation-induced bulk protein turnover depends virtually exclusively...
Höper, Dirk, Völker, Uwe, Hecker, Michael
The σB-dependent general stress regulon of Bacillus subtilis comprises more than 150 members. Induction of this regulon by imposition of environmental or metabolic stress confers multiple,...
Westers, Helga, Braun, Peter G., Westers, Lidia, Antelmann, Haike, Hecker, Michael, Jongbloed, Jan D. H., ...
Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B....
Kirstein, Janine, Zühlke, Daniela, Gerth, Ulf, Turgay, Kürşad, Hecker, Michael
The soil bacterium Bacillus subtilis possesses a fine-tuned and complex heat stress response system. The repressor CtsR, whose activity is regulated by its modulators McsA and McsB, controls the...
Role of the Fur Regulon in Iron Transport in Bacillus subtilis
Ollinger, Juliane, Song, Kyung-Bok, Antelmann, Haike, Hecker, Michael, Helmann, John D.
The Bacillus subtilis ferric uptake regulator (Fur) protein mediates the iron-dependent repression of at least 20 operons encoding ∼40 genes. We investigated the physiological roles of...
Involvement of Bacillus subtilis ClpE in CtsR Degradation and Protein Quality Control
Miethke, Marcus, Hecker, Michael, Gerth, Ulf
The heat-inducible CtsR regulon of Bacillus subtilis codes for three Clp proteins with chaperone or protease activity. While the importance of ClpC and ClpP has been elucidated for a wide range of...
Spx Is a Global Effector Impacting Stress Tolerance and Biofilm Formation in Staphylococcus aureus
Pamp, Sünje Johanna, Frees, Dorte, Engelmann, Susanne, Hecker, Michael, Ingmer, Hanne
In Bacillus subtilis, Spx was recently characterized as a novel type of global regulator whose activity is regulated by the redox status of the cells. In the present study, we demonstrate that...
Völker, Uwe, Maul, Björn, Hecker, Michael
The alternative sigma factor ςB of Bacillus subtilis is required for the induction of approximately 100 genes after the imposition of a whole range of stresses and energy limitation. In this study,...
Tobisch, Steffen, Stülke, Jörg, Hecker, Michael
The lic operon of Bacillus subtilis is required for the transport and degradation of oligomeric β-glucosides, which are produced by extracellular enzymes on substrates such as lichenan or barley...
Petersohn, Anja, Bernhardt, Jörg, Gerth, Ulf, Höper, Dirk, Koburger, Torsten, Völker, Uwe, ...
A consensus-directed search for ςB promoters was used to locate potential candidates for new ςB-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations...
Role of CcpA in Regulation of the Central Pathways of Carbon Catabolism in Bacillus subtilis
Tobisch, Steffen, Zühlke, Daniela, Bernhardt, Jörg, Stülke, Jörg, Hecker, Michael
The Bacillus subtilis two-dimensional (2D) protein index contains almost all glycolytic and tricarboxylic acid (TCA) cycle enzymes, among them the most abundant housekeeping proteins of growing...
The Clp Proteases of Bacillus subtilis Are Directly Involved in Degradation of Misfolded Proteins
Krüger, Elke, Witt, Elke, Ohlmeier, Steffen, Hanschke, Renate, Hecker, Michael
The presence of the heat stress response-related ATPases ClpC and ClpX or the peptidase ClpP in the cell is crucial for tolerance of many forms of stress in Bacillus subtilis. Assays for detection of...
Antelmann, Haike, Scharf, Christian, Hecker, Michael
The phosphate starvation response in Bacillus subtilis was analyzed using two-dimensional (2D) polyacrylamide gel electrophoresis of cell extracts and supernatants from phosphate-starved cells. Most...
Characterization of the ςB Regulon in Staphylococcus aureus
Gertz, Silke, Engelmann, Susanne, Schmid, Roland, Ziebandt, Anne-Kathrin, Tischer, Karsten, Scharf, Christian, ...
The ςB-dependent stress regulon in gram-positive bacteria might fulfill a physiological role in stress response and virulence similar to that of the ςS regulon in Escherichia coli and other...
Putative Sigma Factor SigI (YkoZ) of Bacillus subtilis Is Induced by Heat Shock
Zuber, Ulrich, Drzewiecki, Kathrin, Hecker, Michael
A Bacillus subtilis disruption mutant with a mutation in sigI (formerly ykoZ) shows a temperature-sensitive growth on agar plates. The transcription of the sigI gene is heat shock induced in rich...
Global Analysis of the General Stress Response of Bacillus subtilis
Petersohn, Anja, Brigulla, Matthias, Haas, Stefan, Hoheisel, Jörg D., Völker, Uwe, Hecker, Michael
Gene arrays containing all currently known open reading frames of Bacillus subtilis were used to examine the general stress response of Bacillus. By proteomics, transcriptional analysis, transposon...
Thioredoxin Is an Essential Protein Induced by Multiple Stresses in Bacillus subtilis
Scharf, Christian, Riethdorf, Sabine, Ernst, Henrik, Engelmann, Susanne, Völker, Uwe, Hecker, Michael
Thioredoxin, a small, ubiquitous protein which participates in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, is an essential protein in Bacillus...
Nonnative Proteins Induce Expression of the Bacillus subtilis CIRCE Regulon
Mogk, Axel, Völker, Andrea, Engelmann, Susanne, Hecker, Michael, Schumann, Wolfgang, Völker, Uwe
The chaperone-encoding groESL and dnaK operons constitute the CIRCE regulon of Bacillus subtilis. Both operons are under negative control of the repressor protein HrcA, which interacts with the CIRCE...
One of Two OsmC Homologs in Bacillus subtilis Is Part of the ςB-Dependent General Stress Regulon
Völker, Uwe, Andersen, Kasper Krogh, Antelmann, Haike, Devine, Kevin M., Hecker, Michael
In this report we present the identification and analysis of two Bacillus subtilis genes, yklA and ykzA, which are homologous to the partially RpoS-controlled osmC gene from Escherichia coli. The...
The yvyD Gene of Bacillus subtilis Is under Dual Control of ςB and ςH
Drzewiecki, Kathrin, Eymann, Christine, Mittenhuber, Gerhard, Hecker, Michael
During a search by computer-aided inspection of two-dimensional (2D) protein gels for ςB-dependent general stress proteins exhibiting atypical induction profiles, a protein initially called Hst23...
The Bacillus subtilis clpC operon is regulated by two stress induction pathways relying on either ςB or a class III stress induction mechanism acting at a ςA-like promoter. When the clpC operon was...
Rachid, Shwan, Ohlsen, Knut, Wallner, Ursula, Hacker, Jörg, Hecker, Michael, Ziebuhr, Wilma
Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of a global regulator of the bacterial stress response, the alternative transcription...
Pummi, Tiina, Leskelä, Soile, Wahlström, Eva, Gerth, Ulf, Tjalsma, Harold, Hecker, Michael, ...
Identification and characterization of a suppressor mutation, sup-15, which partially restored secretion in the protein secretion-deficient Bacillus subtilis ecsA26 mutant, led us to discover a novel...
Heat Shock Proteome of Agrobacterium tumefaciens: Evidence for New Control Systems
Rosen, Ran, Büttner, Knut, Becher, Dörte, Nakahigashi, Kenji, Yura, Takashi, Hecker, Michael, ...
The regulation of Agrobacterium tumefaciens heat shock genes involves a transcriptional activator (RpoH) and repressor elements (HrcA-CIRCE). Using proteome analysis and mutants in these control...
Eymann, Christine, Homuth, Georg, Scharf, Christian, Hecker, Michael
The stringent response in Bacillus subtilis was characterized by using proteome and transcriptome approaches. Comparison of protein synthesis patterns of wild-type and relA mutant cells cultivated...
Mäder, Ulrike, Homuth, Georg, Scharf, Christian, Büttner, Knut, Bode, Rüdiger, Hecker, Michael
A comprehensive study of Bacillus subtilis gene expression patterns in response to amino acid availability was performed by means of proteomics and transcriptomics. The methods of two-dimensional...
Lindner, Cordula, Hecker, Michael, Le Coq, Dominique, Deutscher, Josef
The Bacillus subtilis antiterminator LicT regulates the expression of bglPH and bglS, which encode the enzymes for the metabolism of aryl-β-glucosides and the β-glucanase BglS. The N-terminal...
Bandow, Julia Elisabeth, Brötz, Heike, Hecker, Michael
Low concentrations of the RNA polymerase inhibitor rifampin added to an exponentially growing culture of Bacillus subtilis led to an instant inhibition of growth. Survival experiments revealed that...
Binding of σA and σB to Core RNA Polymerase after Environmental Stress in Bacillus subtilis
Rollenhagen, Claudia, Antelmann, Haike, Kirstein, Janine, Delumeau, Olivier, Hecker, Michael, Yudkin, Michael D.
In Bacillus subtilis, the alternative sigma factor σB is activated in response to environmental stress or energy depletion. The general stress regulon under the control of σB provides the cell with...
Clp-mediated proteolysis in Gram-positive bacteria is autoregulated by the stability of a repressor
Krüger, Elke, Zühlke, Daniela, Witt, Elke, Ludwig, Holger, Hecker, Michael
The heat shock proteins ClpC and ClpP are subunits of an ATP-dependent protease of Bacillus subtilis. Under non-stressed conditions, transcription of the clpC and clpP genes is negatively regulated...
Proteomic Approach to Understanding Antibiotic Action
Bandow, Julia Elisabeth, Brötz, Heike, Leichert, Lars Ingo Ole, Labischinski, Harald, Hecker, Michael
We have used proteomic technology to elucidate the complex cellular responses of Bacillus subtilis to antimicrobial compounds belonging to classical and emerging antibiotic classes. We established on...
Global Characterization of Disulfide Stress in Bacillus subtilis
Leichert, Lars Ingo Ole, Scharf, Christian, Hecker, Michael
We used DNA macroarray and proteome analysis to analyze the regulatory networks in Bacillus subtilis that are affected by disulfide stress. To induce disulfide stress, we used the specific thiol...
Kohler, Christian, Von Eiff, Christof, Peters, Georg, Proctor, Richard A., Hecker, Michael, Engelmann, Susanne
The high-resolution two-dimensional (2D) protein gel electrophoresis technique combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry was used for identification...
Fine-Tuning in Regulation of Clp Protein Content in Bacillus subtilis†
Gerth, Ulf, Kirstein, Janine, Mostertz, Jörg, Waldminghaus, Torsten, Miethke, Marcus, Kock, Holger, ...
Clp-controlled proteolysis in Bacillus subtilis seems to play a substantial role, particularly under stress conditions. Calibrated Western blot analyses were used to estimate the approximate numbers...
Mäder, Ulrike, Hennig, Susanne, Hecker, Michael, Homuth, Georg
In Bacillus subtilis, the genes of the branched-chain amino acids biosynthetic pathway are organized in three genetic loci: the ilvBHC-leuABCD (ilv-leu) operon, ilvA, and ilvD. These genes, as well...
Proteomics of Protein Secretion by Bacillus subtilis: Separating the “Secrets” of the Secretome
Tjalsma, Harold, Antelmann, Haike, Jongbloed, Jan D.H., Braun, Peter G., Darmon, Elise, Dorenbos, Ronald, ...
Secretory proteins perform a variety of important “remote-control” functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make...
Bernhardt, Jörg, Weibezahn, Jimena, Scharf, Christian, Hecker, Michael
Dual channel imaging and warping of two-dimensional (2D) protein gels were used to visualize global changes of the gene expression patterns in growing Bacillus subtilis cells during entry into the...
Zanen, Geeske, Antelmann, Haike, Westers, Helga, Hecker, Michael, Van Dijl, Jan Maarten, Quax, Wim J.
Bacillus subtilis contains three proteins of the signal recognition particle-GTPase family known as Ffh, FtsY, and FlhF. Here we show that FlhF is dispensable for protein secretion, unlike Ffh and...
The ClpP Peptidase Is the Major Determinant of Bulk Protein Turnover in Bacillus subtilis
Kock, Holger, Gerth, Ulf, Hecker, Michael
Measurements of overall protein degradation rates in wild-type and clpP mutant Bacillus subtilis cells revealed that stress- or starvation-induced bulk protein turnover depends virtually exclusively...
Höper, Dirk, Völker, Uwe, Hecker, Michael
The σB-dependent general stress regulon of Bacillus subtilis comprises more than 150 members. Induction of this regulon by imposition of environmental or metabolic stress confers multiple,...
Westers, Helga, Braun, Peter G., Westers, Lidia, Antelmann, Haike, Hecker, Michael, Jongbloed, Jan D. H., ...
Small lipases of Bacillus species, such as LipA from Bacillus subtilis, have a high potential for industrial applications. Recent studies showed that deletion of six AT-rich islands from the B....
Kirstein, Janine, Zühlke, Daniela, Gerth, Ulf, Turgay, Kürşad, Hecker, Michael
The soil bacterium Bacillus subtilis possesses a fine-tuned and complex heat stress response system. The repressor CtsR, whose activity is regulated by its modulators McsA and McsB, controls the...
Role of the Fur Regulon in Iron Transport in Bacillus subtilis
Ollinger, Juliane, Song, Kyung-Bok, Antelmann, Haike, Hecker, Michael, Helmann, John D.
The Bacillus subtilis ferric uptake regulator (Fur) protein mediates the iron-dependent repression of at least 20 operons encoding ∼40 genes. We investigated the physiological roles of...
Involvement of Bacillus subtilis ClpE in CtsR Degradation and Protein Quality Control
Miethke, Marcus, Hecker, Michael, Gerth, Ulf
The heat-inducible CtsR regulon of Bacillus subtilis codes for three Clp proteins with chaperone or protease activity. While the importance of ClpC and ClpP has been elucidated for a wide range of...
Spx Is a Global Effector Impacting Stress Tolerance and Biofilm Formation in Staphylococcus aureus
Pamp, Sünje Johanna, Frees, Dorte, Engelmann, Susanne, Hecker, Michael, Ingmer, Hanne
In Bacillus subtilis, Spx was recently characterized as a novel type of global regulator whose activity is regulated by the redox status of the cells. In the present study, we demonstrate that...
Monitoring of stress responses
Schweder, Thomas, Jürgen, Britta, Voigt, Birgit, Pioch, Daniel, Hecker, Michael, Evers, Stefan, ...
Darmon, Elise , Dorenbos, Ronald, Meens, Jochen, Freudl, Roland, Antelmann, Haike, Hecker, Michael, ...
The gram-positive bacterium Bacillus subtilis secretes high levels of proteins into its environment. Most of these secretory proteins are exported from the cytoplasm in an unfolded state and have to...
Rogasch, Kathrin, Rühmling, Vanessa, Pané-Farré, Jan, Höper, Dirk, Weinberg, Christin, Fuchs, Stephan, ...
The two-component system SaeRS consisting of the histidin kinase SaeS and the response regulator SaeR is known to act on virulence gene expression in Staphylococcus aureus. In order to get a more...
Anaerobic Gene Expression in Staphylococcus aureus▿ †
Fuchs, Stephan, Pané-Farré, Jan, Kohler, Christian, Hecker, Michael, Engelmann, Susanne
An investigation of gene expression in Staphylococcus aureus after a switch from aerobic to anaerobic growth was initiated by using the proteomic and transcriptomic approaches. In the absence of...
Integrated network reconstruction, visualization and analysis using YANAsquare
Schwarz, Roland, Liang, Chunguang, Kaleta, Christoph, Kühnel, Mark, Hoffmann, Eik, Kuznetsov, Sergei, ...
The tyrosine kinase McsB is a regulated adaptor protein for ClpCP
Kirstein, Janine, Dougan, David A, Gerth, Ulf, Hecker, Michael, Turgay, Kürşad
Cells of the soil bacterium Bacillus subtilis have to adapt to fast environmental changes in their natural habitat. Here, we characterized a novel system in which cells respond to heat shock by...
Zweers, Jessica C, Barák, Imrich, Becher, Dörte, Driessen, Arnold JM, Hecker, Michael, Kontinen, Vesa P, ...
Gerth, Ulf, Kock, Holger, Kusters, Ilja, Michalik, Stephan, Switzer, Robert L., Hecker, Michael
Entry into stationary phase in Bacillus subtilis is linked not only to a redirection of the gene expression program but also to posttranslational events such as protein degradation. Using 35S-labeled...
Koczan, Dirk, Drynda, Susanne, Hecker, Michael, Drynda, Andreas, Guthke, Reinhard, Kekow, Joern, ...
Galka, Frank, Wai, Sun Nyunt, Kusch, Harald, Engelmann, Susanne, Hecker, Michael, Schmeck, Bernd, ...
Secretion of effector molecules is one of the major mechanisms by which the intracellular human pathogen Legionella pneumophila interacts with host cells during infection. Specific secretion...
Hochgräfe, Falko, Wolf, Carmen, Fuchs, Stephan, Liebeke, Manuel, Lalk, Michael, Engelmann, Susanne, ...
The nonpathogenic Bacillus subtilis and the pathogen Staphylococcus aureus are gram-positive model organisms that have to cope with the radical nitric oxide (NO) generated by nitrite reductases of...
Raberg, Matthias, Reinecke, Frank, Reichelt, Rudolf, Malkus, Ursula, König, Simone, Pötter, Markus, ...
Two-dimensional polyacrylamide gel electrophoresis (2D PAGE), in combination with matrix-assisted laser desorption ionization-time of flight analysis, and the recently revealed genome sequence of...
Kohler, Christian, Von Eiff, Christof, Liebeke, Manuel, McNamara, Peter J., Lalk, Michael, Proctor, Richard A., ...
Both the high-resolution two-dimensional protein gel electrophoresis technique and full-genome DNA microarrays were used for identification of Staphylococcus aureus genes whose expression was changed...
Characterization of the Oxygen-Responsive NreABC Regulon of Staphylococcus aureus▿ †
Schlag, Steffen, Fuchs, Stephan, Nerz, Christiane, Gaupp, Rosmarie, Engelmann, Susanne, Liebeke, Manuel, ...
Here, we investigate the functionality of the oxygen-responsive nitrogen regulation system NreABC in the human pathogen Staphylococcus aureus and evaluate its role in anaerobic gene regulation and...
Wolff, Susanne, Hahne, Hannes, Hecker, Michael, Becher, Dörte
The Gram-positive bacterium Staphylococcus aureus is a serious human pathogen causing a wide variety of diseases, and its increasing resistance toward all available antibiotics makes its further...
Wecke, Tina, Zühlke, Daniela, Mäder, Ulrike, Jordan, Sina, Voigt, Birgit, Pelzer, Stefan, ...
The related lipo(depsi)peptide antibiotics daptomycin and friulimicin B show great potential in the treatment of multiply resistant gram-positive pathogens. Applying genome-wide in-depth expression...
Role of RsbU in Controlling SigB Activity in Staphylococcus aureus following Alkaline Stress▿
Pané-Farré, Jan, Jonas, Beate, Hardwick, Steven W., Gronau, Katrin, Lewis, Richard J., Hecker, Michael, ...
SigB is an alternative sigma factor that controls a large regulon in Staphylococcus aureus. Activation of SigB requires RsbU, a protein phosphatase 2C (PP2C)-type phosphatase. In a closely related...