Michael J. Gilchrist

Evading the annotation bottleneck: using sequence similarity to search non-sequence gene data (2008)

Gilchrist, Michael J, Christensen, Mikkel B, Harland, Richard, Pollet, Nicolas, Smith, James C, Ueno, Naoto, ...

Abstract Background Non-sequence gene data (images, literature, etc.) can be found in many different public databases. Access to these data is mostly by text based methods using gene names; however,...

SNP finding in pig mitochondrial ESTs (2008)

Karsten Scheibye-alsing, Susanna Cirera, Michael J. Gilchrist

SUMMARY: The sino-danish pig genome project produced 685,851 ESTs (Expressed Sequence Tags)(Gorodkin et al. 2007), of which 41,499 originates from the mitochondrial genome. In this study the...

Modelling and measuring single cell RNA expression levels find considerable transcriptional differences among phenotypically identical cells (2008)

Subkhankulova, Tatiana, Gilchrist, Michael J, Livesey, Frederick J

Abstract Background Phenotypically identical cells demonstrate predictable, robust behaviours. However, there is uncertainty as to whether phenotypically identical cells are equally similar at the...

In vivo imaging of oskar mRNA transport reveals the mechanism of posterior localization (2008)

Zimyanin, Vitaly L, Belaya, Katsiaryna, Pecreaux, Jacques, Gilchrist, Michael J, Clark, Alejandra, Davis, Ilan, ...

oskar mRNA localization to the posterior of the Drosophila oocyte defines where the abdomen and germ cells form in the embryo. Although this localization requires microtubules and the plus...

EST analysis on pig mitochondria reveal novel expression differences between developmental and adult tissues (2007)

Scheibye-Alsing, Karsten, Cirera, Susanna, Gilchrist, Michael J, Fredholm, Merete, Gorodkin, Jan

Abstract Background The mitochondria are involved in many basic functions in cells of vertebrates, and can be considered the power generator of the cell. Though the mitochondria have been extensively...

Detection of RNA structures in porcine EST data and related mammals (2007)

Seemann, Stefan E, Gilchrist, Michael J, Hofacker, Ivo L, Stadler, Peter F, Gorodkin, Jan

Abstract Background Non-coding RNAs (ncRNAs) are involved in a wide spectrum of regulatory functions. Within recent years, there have been increasing reports of observed polyadenylated ncRNAs and...

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags (2007)

Gorodkin, Jan, Cirera, Susanna, Hedegaard, Jakob, Gilchrist, Michael J, Panitz, Frank, Jørgensen, Claus, ...

Background Knowledge of the structure of gene expression is essential for mammalian transcriptomics research. We analyzed a collection of more than one million porcine expressed sequence tags (ESTs),...

2007 Gorodkin et Volume al. 8, Issue 4, Article R45 Open Access (2007)

Jan Gorodkin, Susanna Cirera, Jakob Hedegaard, Michael J Gilchrist, Frank Panitz, Claus Jørgensen, ...

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags

Defining Synphenotype Groups in Xenopus tropicalis by Use of Antisense Morpholino Oligonucleotides (2006)

Amer Ahmed Rana, Clara Collart, Michael J. Gilchrist, J. C. Smith

To identify novel genes involved in early development, and as proof-of-principle of a large-scale reverse genetics approach in a vertebrate embryo, we have carried out an antisense morpholino...

Defining Synphenotype Groups in Xenopus tropicalis by Use of Antisense Morpholino Oligonucleotides

Rana, Amer Ahmed, Collart, Clara, Gilchrist, Michael J, Smith, J. C

To identify novel genes involved in early development, and as proof-of-principle of a large-scale reverse genetics approach in a vertebrate embryo, we have carried out an antisense morpholino...

Defining Synphenotype Groups in Xenopus tropicalis by Use of Antisense Morpholino Oligonucleotides

Rana, Amer Ahmed, Collart, Clara, Gilchrist, Michael J, Smith, J. C

To identify novel genes involved in early development, and as proof-of-principle of a large-scale reverse genetics approach in a vertebrate embryo, we have carried out an antisense morpholino...

Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 expressed sequence tags

Gorodkin, Jan, Cirera, Susanna, Hedegaard, Jakob, Gilchrist, Michael J, Panitz, Frank, Jørgensen, Claus, ...

A resource consisting of one million porcine ESTs is described, providing an essential resource for annotation, comparative genomics, assembly of the pig genome sequence, and further porcine...

Maternal Argonaute 2 Is Essential for Early Mouse Development at the Maternal-Zygotic Transition

Lykke-Andersen, Karin, Gilchrist, Michael J., Grabarek, Joanna B., Das, Partha, Miska, Eric, Zernicka-Goetz, Magdalena

Activation of zygotic gene expression in the two-cell mouse embryo is associated with destruction of maternally inherited transcripts, an important process for embryogenesis about which little is...

In Vivo Imaging of oskar mRNA Transport Reveals the Mechanism of Posterior Localization

Zimyanin, Vitaly L., Belaya, Katsiaryna, Pecreaux, Jacques, Gilchrist, Michael J., Clark, Alejandra, Davis, Ilan, ...

oskar mRNA localization to the posterior of the Drosophila oocyte defines where the abdomen and germ cells form in the embryo. Although this localization requires microtubules and the plus...

Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo

Von Hofsten, Jonas, Elworthy, Stone, Gilchrist, Michael J, Smith, James C, Wardle, Fiona C, Ingham, Philip W

The zebrafish u-boot (ubo) gene encodes the transcription factor Prdm1, which is essential for the specification of the primary slow-twitch muscle fibres that derive from adaxial cells. Here, we show...

A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation

Morley, Rosalind H., Lachani, Kim, Keefe, Damian, Gilchrist, Michael J., Flicek, Paul, Smith, James C., ...

Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We...