Michael J. O'Hare

CLONAL ANALYSIS OF EXPRESSION OF EPITHELIAL ANTIGENS IN CULTURES OF NORMAL HUMAN BREAST (2008)

Isobel M. Brooks, Hilary J, A. Virginia Foster, Morag L. Ellison, Michael J. O'hare

Cells from normal human breast epithelium were cloned in monolayer culture and the clones were stained with monoclonal antibodies. Tissue was from reduction mammoplasty operations. Cloning...

Establishment of the epithelial-specific transcriptome of normal and malignant human breast cells based on MPSS and array expression data (2006)

Grigoriadis, Anita, Mackay, Alan, Reis-Filho, Jorge S, Steele, Dawn, Iseli, Christian, Stevenson, Brian J, ...

Abstract Introduction Diverse microarray and sequencing technologies have been widely used to characterise the molecular changes in malignant epithelial cells in breast cancers. Such gene expression...

Expression profiling of purified normal human luminal and myoepithelial breast cells: Identification of novel prognostic markers for breast cancer (2004)

Jones, Chris, Mackay, Alan, Grigoriadis, Anita, Cossu, Antonio, Reis-Filho, Jorge S., Fulford, Laura, ...

The normal duct-lobular system of the breast is lined by two epithelial cell types, inner luminal secretory cells and outer contractile myoepithelial cells. We have generated comprehensive expression...

Models of breast cancer: is merging human and animal models the future? (2003)

Kim, Jong B, O'Hare, Michael J, Stein, Robert

Abstract Survival rates of patients with early breast cancer in the United Kingdom and in the United States have improved steadily over the past 15 years. The only way to continue or even accelerate...

The mammary myoepithelial cell - Cinderella or ugly sister? (2000)

Lakhani, Sunil R, O'Hare, Michael J

Abstract The breast myoepithelial cell is the Cinderella of mammary biology. Although its contribution to benign and some malignant pathologies is recognised, it has been largely neglected in...

Metabolic activation of the food mutagens 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ) to DNA binding species in human mammary epithelial cells (1992)

Pfau, Wolfgang, O'Hare, Michael J., Grover, Philip L., Phillips, David H.

When incubated in suspension with the heterocyclic aromatic amine food mutagens 2-amino-3-methylimidazo [4,5-f]quinoline (IQ) and 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), human mammary...

Conditional immortalization of freshly isolated human mammary fibroblasts and endothelial cells

O'Hare, Michael J., Bond, Jacquelyn, Clarke, Catherine, Takeuchi, Yasuhiro, Atherton, Amanda J., Berry, Catherine, ...

Reports differ as to whether reconstitution of telomerase activity alone is sufficient for immortalization of different types of human somatic cells or whether additional activities encoded by other...

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags

Dias Neto, Emmanuel, Garcia Correa, Ricardo, Verjovski-Almeida, Sergio, Briones, Marcelo R. S., Nagai, Maria Aparecida, Da Silva, Wilson, ...

Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription–PCR using arbitrarily chosen primers will result in the preferential amplification of...

The mammary myoepithelial cell - Cinderella or ugly sister?

Lakhani, Sunil R, O'Hare, Michael J

The breast myoepithelial cell is the Cinderella of mammary biology. Although its contribution to benign and some malignant pathologies is recognised, it has been largely neglected in molecular and...

Comprehensive sampling of gene expression in human cell lines with massively parallel signature sequencing

Jongeneel, C. Victor, Iseli, Christian, Stevenson, Brian J., Riggins, Gregory J., Lal, Anita, Mackay, Alan, ...

Whereas information is rapidly accumulating about the structure and position of genes encoded in the human genome, less is known about the complexity and relative abundance of their expression in...

Cyclin-dependent kinase 5 is a mediator of dopaminergic neuron loss in a mouse model of Parkinson's disease

Smith, Patrice D., Crocker, Stephen J., Jackson-Lewis, Vernice, Jordan-Sciutto, Kelly L., Hayley, Shawn, Mount, Matthew P., ...

Recent evidence indicates that cyclin-dependent kinases (CDKs, cdks) may be inappropriately activated in several neurodegenerative conditions. Here, we report that cdk5 expression and activity are...

Models of breast cancer: is merging human and animal models the future?

Kim, Jong B, O'Hare, Michael J, Stein, Robert

Survival rates of patients with early breast cancer in the United Kingdom and in the United States have improved steadily over the past 15 years. The only way to continue or even accelerate this...

Conditional immortalization of freshly isolated human mammary fibroblasts and endothelial cells

O'Hare, Michael J., Bond, Jacquelyn, Clarke, Catherine, Takeuchi, Yasuhiro, Atherton, Amanda J., Berry, Catherine, ...

Reports differ as to whether reconstitution of telomerase activity alone is sufficient for immortalization of different types of human somatic cells or whether additional activities encoded by other...

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags

Dias Neto, Emmanuel, Garcia Correa, Ricardo, Verjovski-Almeida, Sergio, Briones, Marcelo R. S., Nagai, Maria Aparecida, Da Silva, Wilson, ...

Theoretical considerations predict that amplification of expressed gene transcripts by reverse transcription–PCR using arbitrarily chosen primers will result in the preferential amplification of...

The mammary myoepithelial cell - Cinderella or ugly sister?

Lakhani, Sunil R, O'Hare, Michael J

The breast myoepithelial cell is the Cinderella of mammary biology. Although its contribution to benign and some malignant pathologies is recognised, it has been largely neglected in molecular and...

Comprehensive sampling of gene expression in human cell lines with massively parallel signature sequencing

Jongeneel, C. Victor, Iseli, Christian, Stevenson, Brian J., Riggins, Gregory J., Lal, Anita, Mackay, Alan, ...

Whereas information is rapidly accumulating about the structure and position of genes encoded in the human genome, less is known about the complexity and relative abundance of their expression in...

Cyclin-dependent kinase 5 is a mediator of dopaminergic neuron loss in a mouse model of Parkinson's disease

Smith, Patrice D., Crocker, Stephen J., Jackson-Lewis, Vernice, Jordan-Sciutto, Kelly L., Hayley, Shawn, Mount, Matthew P., ...

Recent evidence indicates that cyclin-dependent kinases (CDKs, cdks) may be inappropriately activated in several neurodegenerative conditions. Here, we report that cdk5 expression and activity are...

Models of breast cancer: is merging human and animal models the future?

Kim, Jong B, O'Hare, Michael J, Stein, Robert

Survival rates of patients with early breast cancer in the United Kingdom and in the United States have improved steadily over the past 15 years. The only way to continue or even accelerate this...

Immortalization of Human Alveolar Epithelial Cells to Investigate Nanoparticle Uptake

Kemp, Sarah J., Thorley, Andrew J., Gorelik, Julia, Seckl, Michael J., O'Hare, Michael J., Arcaro, Alexandre, ...

Primary human alveolar type 2 (AT2) cells were immortalized by transduction with the catalytic subunit of telomerase and simian virus 40 large-tumor antigen. Characterization by immunochemical and...