Michael Knop

Human Lsg1 defines a family of essential GTPases that correlates with the evolution of compartmentalization (2005)

Reynaud, Emmanuel G, Andrade, Miguel A, Bonneau, Fabien, Ly, Thi, Knop, Michael, Scheffzek, Klaus, ...

Abstract Background Compartmentalization is a key feature of eukaryotic cells, but its evolution remains poorly understood. GTPases are the oldest enzymes that use nucleotides as substrates and they...

Spc98p Directs the Yeast γ-Tubulin Complex into the Nucleus and Is Subject to Cell Cycle-dependent Phosphorylation on the Nuclear Side of the Spindle Pole Body

Pereira, Gislene, Knop, Michael, Schiebel, Elmar

In the yeast Saccharomyces cerevisiae, microtubules are organized by the spindle pole body (SPB), which is embedded in the nuclear envelope. Microtubule organization requires the γ-tubulin complex...

Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication

Elliott, Sarah, Knop, Michael, Schlenstedt, Gabriel, Schiebel, Elmar

In yeast, microtubules are organized by the spindle pole body (SPB). The SPB is a disk-like multilayered structure that is embedded in the nuclear envelope via its central plaque, whereas the outer...

Prospore membrane formation linked to the leading edge protein (LEP) coat assembly

Moreno-Borchart, Alexandra C., Strasser, Katrin, Finkbeiner, Martin G., Shevchenko, Anna, Shevchenko, Andrej, Knop, Michael

In yeast, the differentiation process at the end of meiosis generates four daughter cells inside the boundaries of the mother cell. A meiosis-specific plaque (MP) at the spindle pole bodies (SPBs)...

Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions

Maekawa, Hiromi, Usui, Takeo, Knop, Michael, Schiebel, Elmar

The budding yeast spindle aligns along the mother– bud axis through interactions between cytoplasmic microtubules (CMs) and the cell cortex. Kar9, in complex with the EB1-related protein Bim1,...

Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis

Knop, Michael, Strasser, Katrin

Spindle pole bodies (SPBs) are the centrosome equivalents in yeast, required for microtubule organization. In yeast, the SPB further serves as the attachment sites of the prospore membrane during...

Molecular Interactions Position Mso1p, a Novel PTB Domain Homologue, in the Interface of the Exocyst Complex and the Exocytic SNARE Machinery in Yeast

Knop, Michael, Miller, K. Juha, Mazza, Massimiliano, Feng, DeJiang, Weber, Marion, Keränen, Sirkka, ...

In this study, we have analyzed the association of the Sec1p interacting protein Mso1p with the membrane fusion machinery in yeast. We show that Mso1p is essential for vesicle fusion during prospore...

Spc98p Directs the Yeast γ-Tubulin Complex into the Nucleus and Is Subject to Cell Cycle-dependent Phosphorylation on the Nuclear Side of the Spindle Pole Body

Pereira, Gislene, Knop, Michael, Schiebel, Elmar

In the yeast Saccharomyces cerevisiae, microtubules are organized by the spindle pole body (SPB), which is embedded in the nuclear envelope. Microtubule organization requires the γ-tubulin complex...

Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication

Elliott, Sarah, Knop, Michael, Schlenstedt, Gabriel, Schiebel, Elmar

In yeast, microtubules are organized by the spindle pole body (SPB). The SPB is a disk-like multilayered structure that is embedded in the nuclear envelope via its central plaque, whereas the outer...

Prospore membrane formation linked to the leading edge protein (LEP) coat assembly

Moreno-Borchart, Alexandra C., Strasser, Katrin, Finkbeiner, Martin G., Shevchenko, Anna, Shevchenko, Andrej, Knop, Michael

In yeast, the differentiation process at the end of meiosis generates four daughter cells inside the boundaries of the mother cell. A meiosis-specific plaque (MP) at the spindle pole bodies (SPBs)...

Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions

Maekawa, Hiromi, Usui, Takeo, Knop, Michael, Schiebel, Elmar

The budding yeast spindle aligns along the mother– bud axis through interactions between cytoplasmic microtubules (CMs) and the cell cortex. Kar9, in complex with the EB1-related protein Bim1,...

Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis

Knop, Michael, Strasser, Katrin

Spindle pole bodies (SPBs) are the centrosome equivalents in yeast, required for microtubule organization. In yeast, the SPB further serves as the attachment sites of the prospore membrane during...

Molecular Interactions Position Mso1p, a Novel PTB Domain Homologue, in the Interface of the Exocyst Complex and the Exocytic SNARE Machinery in Yeast

Knop, Michael, Miller, K. Juha, Mazza, Massimiliano, Feng, DeJiang, Weber, Marion, Keränen, Sirkka, ...

In this study, we have analyzed the association of the Sec1p interacting protein Mso1p with the membrane fusion machinery in yeast. We show that Mso1p is essential for vesicle fusion during prospore...

Nud1p, the yeast homolog of Centriolin, regulates spindle pole body inheritance in meiosis

Gordon, Oren, Taxis, Christof, Keller, Philipp J, Benjak, Aleksander, Stelzer, Ernst H K, Simchen, Giora, ...

Nud1p, a protein homologous to the mammalian centrosome and midbody component Centriolin, is a component of the budding yeast spindle pole body (SPB), with roles in anchorage of microtubules and...

Reconstitution of Mdm2-Dependent Post-Translational Modifications of p53 in Yeast

Di Ventura, Barbara, Funaya, Charlotta, Antony, Claude, Knop, Michael, Serrano, Luis

p53 mediates cell cycle arrest or apoptosis in response to DNA damage. Its activity is subject to a tight regulation involving a multitude of post-translational modifications. The plethora of...

Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system

Taxis, Christof, Keller, Philipp, Kavagiou, Zaharoula, Jensen, Lars Juhl, Colombelli, Julien, Bork, Peer, ...

Spindle pole bodies (SPBs) provide a structural basis for genome inheritance and spore formation during meiosis in yeast. Upon carbon source limitation during sporulation, the number of haploid...

Cytokinesis in yeast meiosis depends on the regulated removal of Ssp1p from the prospore membrane

Maier, Peter, Rathfelder, Nicole, Finkbeiner, Martin G, Taxis, Christof, Mazza, Massimiliano, Panse, Sophie Le, ...

Intracellular budding is a developmentally regulated type of cell division common to many fungi and protists. In Saccaromyces cerevisiae, intracellular budding requires the de novo assembly of...

Efficient protein depletion by genetically controlled deprotection of a dormant N-degron

Taxis, Christof, Stier, Gunter, Spadaccini, Roberta, Knop, Michael

Methods that allow for the manipulation of genes or their products have been highly fruitful for biomedical research. Here, we describe a method that allows the control of protein abundance by a...

Evolution of Mutational Robustness in the Yeast Genome: A Link to Essential Genes and Meiotic Recombination Hotspots

Keller, Philipp J., Knop, Michael

Deleterious mutations inevitably emerge in any evolutionary process and are speculated to decisively influence the structure of the genome. Meiosis, which is thought to play a major role in handling...

The SpoMBe pathway drives membrane bending necessary for cytokinesis and spore formation in yeast meiosis

Maier, Peter, Rathfelder, Nicole, Maeder, Celine I, Colombelli, Julien, Stelzer, Ernst H K, Knop, Michael

Precise control over organelle shapes is essential for cellular organization and morphogenesis. During yeast meiosis, prospore membranes (PSMs) constitute bell-shaped organelles that enwrap the...