The Metal-Dependent Regulators FurA and FurB from Mycobacterium Tuberculosis (2008)
Debora Lucarelli, Michael L. Vasil, Wolfram Meyer-Klaucke, Ehmke Pohl
The ferric uptake regulators (Fur) form a large family of bacterial metalactivated DNA-binding proteins that control a diverse set of genes at the transcriptional level. Mycobacterium tuberculosis,...
FpvB, an alternative type I ferripyoverdine receptor of Pseudomonas aeruginosa (2004)
Ghysels, Bart, Dieu, Bui Thi Min, Beatson, Scott A., Pirnay, Jean-Paul, Ochsner, Urs A., Vasil, Michael L., ...
Under conditions of iron limitation, Pseudomonas aeruginosa secretes a high-affinity siderophore pyoverdine to scavenge Fe(III) in the extracellular environment and shuttle it into the cell. Uptake...
FpvB, an alternative type I ferripyoverdine receptor of Pseudomonas aeruginosa (2004)
Ghysels, Bart, Dieu, Bui Thi Min, Beatson, Scott A., Pirnay, Jean-Paul, Ochsner, Urs A., Vasil, Michael L., ...
Under conditions of iron limitation, Pseudomonas aeruginosa secretes a high-affinity siderophore pyoverdine to scavenge Fe(III) in the extracellular environment and shuttle it into the cell. Uptake...
In Pseudomonas Aeruginosa, Urs A. Ochsner, Daniel J. Hassett, Michael L. Vasil
The ohr (organic hydroperoxide resistance) gene product of Pseudomonas aeruginosa was essential for optimal resistance to organic hydroperoxides (OHPs) but not to hydrogen peroxide or paraquat. A...
Pseudomonas aeruginosa fur Overlaps with a Gene Encoding a Novel Outer Membrane Lipoprotein, OmlA
Ochsner, Urs A., Vasil, Adriana I., Johnson, Zaiga, Vasil, Michael L.
A novel outer membrane lipoprotein in Pseudomonas aeruginosa is encoded by the omlA gene, which was identified immediately upstream of the fur (ferric uptake regulator) gene. The omlA and fur genes...
Ma, Ju-Fang, Ochsner, Urs A., Klotz, Martin G., Nanayakkara, Vagira K., Howell, Michael L., Johnson, Zaiga, ...
We have cloned a 3.6-kb genomic DNA fragment from Pseudomonas aeruginosa harboring the rpoA, rplQ, katA, and bfrA genes. These loci are predicted to encode, respectively, (i) the α subunit of RNA...
Stintzi, Alain, Johnson, Zaiga, Stonehouse, Martin, Ochsner, Urs, Meyer, Jean-Marie, Vasil, Michael L., ...
A putative operon of four genes implicated in the synthesis of the chromophore moiety of the Pseudomonas aeruginosa siderophore pyoverdine, dubbed pvcABCD (where pvc stands for pyoverdine...
Ochsner, Urs A., Vasil, Michael L., Alsabbagh, Eyad, Parvatiyar, Kislay, Hassett, Daniel J.
Pseudomonas aeruginosa possesses an extensive armament of genes involved in oxidative stress defense, including katB-ankB, ahpB, and ahpC-ahpF. Transcription of these genes was regulated in response...
Ochsner, Urs A., Hassett, Daniel J., Vasil, Michael L.
The ohr (organic hydroperoxide resistance) gene product of Pseudomonas aeruginosa was essential for optimal resistance to organic hydroperoxides (OHPs) but not to hydrogen peroxide or paraquat. A...
Wilderman, Paula J., Vasil, Adriana I., Johnson, Zaiga, Wilson, Megan J., Cunliffe, Heather E., Lamont, Iain L., ...
The expression of many virulence factors in Pseudomonas aeruginosa is dependent upon environmental conditions, including iron levels, oxygen, temperature, and osmolarity. The virulence of P....
Vasil, Michael L., Ochsner, Urs A., Johnson, Zaiga, Colmer, Jane A., Hamood, Abdul N.
We previously identified a novel regulator of the exotoxin A gene (toxA) in Pseudomonas aeruginosa, PtxR, that belongs to the LysR family of prokaryotic regulatory proteins. Preliminary data also...
El-Zaim, Haissam S., Chopra, Ashok K., Peterson, Johnny W., Vasil, Michael L., Heggers, John P.
Pseudomonas aeruginosa is an opportunistic pathogen that causes serious and sometimes fatal infections in the compromised host, especially in patients with major trauma or thermal injuries. Exotoxin...
Pseudomonas aeruginosa Hemolytic Phospholipase C Suppresses Neutrophil Respiratory Burst Activity
Terada, Lance S., Johansen, Kristine A., Nowbar, Sogol, Vasil, Adriana I., Vasil, Michael L.
Pseudomonas aeruginosa is a persistent pathogen in the airways of patients with cystic fibrosis or bronchiectasis from other causes and appears to have evolved strategies to survive the inflammatory...
Ochsner, Urs A., Snyder, Aleksandra, Vasil, Adriana I., Vasil, Michael L.
A novel secretion pathway originally found in plants has recently been discovered in bacteria and termed TAT, for “twin-arginine translocation,” with respect to the presence of an Arg-Arg motif...
Siderophore-mediated signaling regulates virulence factor production in Pseudomonas aeruginosa
Lamont, Iain L., Beare, Paul A., Ochsner, Urs, Vasil, Adriana I., Vasil, Michael L.
Numerous bacteria secrete low molecular weight compounds termed siderophores that have a high affinity for iron ions. Siderophores have a well-documented role as iron-scavenging chemicals, chelating...
Involvement of the twin-arginine translocation system in protein secretion via the type II pathway
Voulhoux, Romé, Ball, Geneviève, Ize, Bérengère, Vasil, Michael L., Lazdunski, Andrée, Wu, Long-Fei, ...
The general secretory pathway (GSP) is a two-step process for the secretion of proteins by Gram-negative bacteria. The translocation across the outer membrane is carried out by the type II system,...
Wieland, Catharina W., Siegmund, Britta, Senaldi, Giorgio, Vasil, Michael L., Dinarello, Charles A., Fantuzzi, Giamila
Chronic pulmonary infection with Pseudomonas aeruginosa is common in cystic fibrosis (CF) patients. P. aeruginosa lipopolysaccharide (LPS), phosholipase C (PLC), and exotoxin A (ETA) were evaluated...
Wilderman, Paula J., Vasil, Adriana I., Martin, Wesley E., Murphy, Robert C., Vasil, Michael L.
Phosphatidylcholine (PC) is a ubiquitous membrane lipid in eukaryotes but has been found in only a limited number of prokaryotes. Both eukaryotes and prokaryotes synthesize PC by methylating...
Cloning of a Phosphate-Regulated Hemolysin Gene (Phospholipase C) from Pseudomonas aeruginosa
Vasil, Michael L., Berka, Randy M., Gray, Gregory L., Nakai, Hiroshi
Phospholipase C (heat-labile hemolysin) of Pseudomonas aeruginosa is a phosphate (Pi)-regulated extracellular protein which may be a significant virulence factor of this organism. The gene for this...
Studies on Toxinogenesis in Vibrio cholerae II. An In Vitro Test for Enterotoxin Production
Vasil, Michael L., Holmes, Randall K., Finkelstein, Richard A.
An Elek test for enterotoxin-producing strains of Vibrio cholerae is described. Thirty-five out of 37 strains of classical V. cholerae produced positive reactions, but only 1 of 69 E1 Tor vibrio...
Wilderman, Paula J., Sowa, Nathaniel A., FitzGerald, David J., FitzGerald, Peter C., Gottesman, Susan, Ochsner, Urs A., ...
In many bacteria, iron homeostasis is controlled primarily by the ferric uptake regulator (Fur), a transcriptional repressor. However, some genes, including those involved in iron storage, are...
Enhanced Pseudomonas aeruginosa Biofilm Development Mediated by Human Neutrophils
Walker, Travis S., Tomlin, Kerry L., Worthen, G. Scott, Poch, Katie R., Lieber, Jonathan G., Saavedra, Milene T., ...
Cystic fibrosis (CF) lung disease features persistent neutrophil accumulation to the airways from the time of infancy. CF children are frequently exposed to Pseudomonas aeruginosa, and by adulthood,...
Iron and Pseudomonas aeruginosa biofilm formation
Banin, Ehud, Vasil, Michael L., Greenberg, E. Peter
Iron serves as a signal in Pseudomonas aeruginosa biofilm development. We examined the influence of mutations in known and putative iron acquisition-signaling genes on biofilm morphology. In...
Chen, Yuxin, Mant, Colin T., Farmer, Susan W., Hancock, Robert E. W., Vasil, Michael L., Hodges, Robert S.
In the present study, the 26-residue peptide sequence Ac-KWKSFLKTFKSAVKTVLHTALKAISS-amide (V681) was utilized as the framework to study the effects of peptide hydrophobicity/hydrophilicity,...
Snyder, Aleksandra, Vasil, Adriana I., Zajdowicz, Sheryl L., Wilson, Zachary R., Vasil, Michael L.
The secretion of PlcH and its homolog PlcN of Pseudomonas aeruginosa through the inner membrane depends upon a functional twin arginine translocase (Tat) system and a Tat signal sequence. Conserved...
Pseudomonas aeruginosa fur Overlaps with a Gene Encoding a Novel Outer Membrane Lipoprotein, OmlA
Ochsner, Urs A., Vasil, Adriana I., Johnson, Zaiga, Vasil, Michael L.
A novel outer membrane lipoprotein in Pseudomonas aeruginosa is encoded by the omlA gene, which was identified immediately upstream of the fur (ferric uptake regulator) gene. The omlA and fur genes...
Ma, Ju-Fang, Ochsner, Urs A., Klotz, Martin G., Nanayakkara, Vagira K., Howell, Michael L., Johnson, Zaiga, ...
We have cloned a 3.6-kb genomic DNA fragment from Pseudomonas aeruginosa harboring the rpoA, rplQ, katA, and bfrA genes. These loci are predicted to encode, respectively, (i) the α subunit of RNA...
Stintzi, Alain, Johnson, Zaiga, Stonehouse, Martin, Ochsner, Urs, Meyer, Jean-Marie, Vasil, Michael L., ...
A putative operon of four genes implicated in the synthesis of the chromophore moiety of the Pseudomonas aeruginosa siderophore pyoverdine, dubbed pvcABCD (where pvc stands for pyoverdine...
Ochsner, Urs A., Vasil, Michael L., Alsabbagh, Eyad, Parvatiyar, Kislay, Hassett, Daniel J.
Pseudomonas aeruginosa possesses an extensive armament of genes involved in oxidative stress defense, including katB-ankB, ahpB, and ahpC-ahpF. Transcription of these genes was regulated in response...
Ochsner, Urs A., Hassett, Daniel J., Vasil, Michael L.
The ohr (organic hydroperoxide resistance) gene product of Pseudomonas aeruginosa was essential for optimal resistance to organic hydroperoxides (OHPs) but not to hydrogen peroxide or paraquat. A...
Wilderman, Paula J., Vasil, Adriana I., Johnson, Zaiga, Wilson, Megan J., Cunliffe, Heather E., Lamont, Iain L., ...
The expression of many virulence factors in Pseudomonas aeruginosa is dependent upon environmental conditions, including iron levels, oxygen, temperature, and osmolarity. The virulence of P....
Vasil, Michael L., Ochsner, Urs A., Johnson, Zaiga, Colmer, Jane A., Hamood, Abdul N.
We previously identified a novel regulator of the exotoxin A gene (toxA) in Pseudomonas aeruginosa, PtxR, that belongs to the LysR family of prokaryotic regulatory proteins. Preliminary data also...
El-Zaim, Haissam S., Chopra, Ashok K., Peterson, Johnny W., Vasil, Michael L., Heggers, John P.
Pseudomonas aeruginosa is an opportunistic pathogen that causes serious and sometimes fatal infections in the compromised host, especially in patients with major trauma or thermal injuries. Exotoxin...
Pseudomonas aeruginosa Hemolytic Phospholipase C Suppresses Neutrophil Respiratory Burst Activity
Terada, Lance S., Johansen, Kristine A., Nowbar, Sogol, Vasil, Adriana I., Vasil, Michael L.
Pseudomonas aeruginosa is a persistent pathogen in the airways of patients with cystic fibrosis or bronchiectasis from other causes and appears to have evolved strategies to survive the inflammatory...
Ochsner, Urs A., Snyder, Aleksandra, Vasil, Adriana I., Vasil, Michael L.
A novel secretion pathway originally found in plants has recently been discovered in bacteria and termed TAT, for “twin-arginine translocation,” with respect to the presence of an Arg-Arg motif...
Siderophore-mediated signaling regulates virulence factor production in Pseudomonas aeruginosa
Lamont, Iain L., Beare, Paul A., Ochsner, Urs, Vasil, Adriana I., Vasil, Michael L.
Numerous bacteria secrete low molecular weight compounds termed siderophores that have a high affinity for iron ions. Siderophores have a well-documented role as iron-scavenging chemicals, chelating...
Involvement of the twin-arginine translocation system in protein secretion via the type II pathway
Voulhoux, Romé, Ball, Geneviève, Ize, Bérengère, Vasil, Michael L., Lazdunski, Andrée, Wu, Long-Fei, ...
The general secretory pathway (GSP) is a two-step process for the secretion of proteins by Gram-negative bacteria. The translocation across the outer membrane is carried out by the type II system,...
Wieland, Catharina W., Siegmund, Britta, Senaldi, Giorgio, Vasil, Michael L., Dinarello, Charles A., Fantuzzi, Giamila
Chronic pulmonary infection with Pseudomonas aeruginosa is common in cystic fibrosis (CF) patients. P. aeruginosa lipopolysaccharide (LPS), phosholipase C (PLC), and exotoxin A (ETA) were evaluated...
Wilderman, Paula J., Vasil, Adriana I., Martin, Wesley E., Murphy, Robert C., Vasil, Michael L.
Phosphatidylcholine (PC) is a ubiquitous membrane lipid in eukaryotes but has been found in only a limited number of prokaryotes. Both eukaryotes and prokaryotes synthesize PC by methylating...
Cloning of a Phosphate-Regulated Hemolysin Gene (Phospholipase C) from Pseudomonas aeruginosa
Vasil, Michael L., Berka, Randy M., Gray, Gregory L., Nakai, Hiroshi
Phospholipase C (heat-labile hemolysin) of Pseudomonas aeruginosa is a phosphate (Pi)-regulated extracellular protein which may be a significant virulence factor of this organism. The gene for this...
Studies on Toxinogenesis in Vibrio cholerae II. An In Vitro Test for Enterotoxin Production
Vasil, Michael L., Holmes, Randall K., Finkelstein, Richard A.
An Elek test for enterotoxin-producing strains of Vibrio cholerae is described. Thirty-five out of 37 strains of classical V. cholerae produced positive reactions, but only 1 of 69 E1 Tor vibrio...
Wilderman, Paula J., Sowa, Nathaniel A., FitzGerald, David J., FitzGerald, Peter C., Gottesman, Susan, Ochsner, Urs A., ...
In many bacteria, iron homeostasis is controlled primarily by the ferric uptake regulator (Fur), a transcriptional repressor. However, some genes, including those involved in iron storage, are...
Enhanced Pseudomonas aeruginosa Biofilm Development Mediated by Human Neutrophils
Walker, Travis S., Tomlin, Kerry L., Worthen, G. Scott, Poch, Katie R., Lieber, Jonathan G., Saavedra, Milene T., ...
Cystic fibrosis (CF) lung disease features persistent neutrophil accumulation to the airways from the time of infancy. CF children are frequently exposed to Pseudomonas aeruginosa, and by adulthood,...
Iron and Pseudomonas aeruginosa biofilm formation
Banin, Ehud, Vasil, Michael L., Greenberg, E. Peter
Iron serves as a signal in Pseudomonas aeruginosa biofilm development. We examined the influence of mutations in known and putative iron acquisition-signaling genes on biofilm morphology. In...
Snyder, Aleksandra, Vasil, Adriana I., Zajdowicz, Sheryl L., Wilson, Zachary R., Vasil, Michael L.
The secretion of PlcH and its homolog PlcN of Pseudomonas aeruginosa through the inner membrane depends upon a functional twin arginine translocase (Tat) system and a Tat signal sequence. Conserved...
Role of Peptide Hydrophobicity in the Mechanism of Action of α-Helical Antimicrobial Peptides▿
Chen, Yuxin, Guarnieri, Michael T., Vasil, Adriana I., Vasil, Michael L., Mant, Colin T., Hodges, Robert S.
In the present study, the 26-residue amphipathic α-helical antimicrobial peptide V13KL (Y. Chen et al., J. Biol. Chem. 2005, 280:12316-12329, 2005) was used as the framework to study the effects of...
Parker, Sarah K., Curtin, Kathryn M., Vasil, Michael L.
We describe mycobacterial phospholipase A activity (MPLA) and, using reverse genetics, have associated this activity with putative mycobacterial cutinase. PLAs, which hydrolyze fatty acids on...
Miller, Rhea M., Tomaras, Andrew P., Barker, Adam P., Voelker, Dennis R., Chan, Edward D., Vasil, Adriana I., ...
Pseudomonas aeruginosa demonstrates type IV pilus-mediated directional twitching motility up a gradient of phosphatidylethanolamine (PE). Only one of four extracellular phospholipases C of P....
Parker, Sarah K., Barkley, Robert M., Rino, John G., Vasil, Michael L.
The cell wall of M. tuberculosis is central to its success as a pathogen. Mycolic acids are key components of this cell wall. The genes involved in joining the α and mero mycolates are located in a...
The Metal-Dependent Regulators FurA and FurB from Mycobacterium Tuberculosis
Lucarelli, Debora, Vasil, Michael L., Meyer-Klaucke, Wolfram, Pohl, Ehmke
The ferric uptake regulators (Fur) form a large family of bacterial metal-activated DNA-binding proteins that control a diverse set of genes at the transcriptional level. Mycobacterium tuberculosis,...
Vasil, Michael L., Stonehouse, Martin J., Vasil, Adriana I., Wadsworth, Sandra J., Goldfine, Howard, Bolcome, Robert E., ...
The hemolytic phospholipase C (PlcHR) expressed by Pseudomonas aeruginosa is the original member of a Phosphoesterase Superfamily, which includes phosphorylcholine-specific phospholipases C (PC-PLC)...
Oglesby, Amanda G., Farrow, John M., Lee, Joon-Hee, Tomaras, Andrew P., Greenberg, E. P., Pesci, Everett C., ...
In iron-replete environments, the Pseudomonas aeruginosa Fur (ferric uptake regulator) protein represses expression of two small regulatory RNAs encoded by prrF1 and prrF2. Here we describe the...