Quantification of Cell Movement Reveals Distinct Edge Motility Types During Cell Spreading (2007)
Jake M. Hofman, Harry V. Xenias, Ingrid A. Spielman, Anna H. Shneidman, Lawrence David, ...
Actin-based motility is central to cellular processes such as migration, bacterial engulfment, and cancer metastasis, and requires precise spatial and temporal regulation of the cytoskeleton. We...
* These authors contributed equally to this work (2007)
Yuguang Xiong, Padmini Rangamani, Benjamin Dubin-thaler, Michael P. Sheetz, Ravi Iyengar, Ravi Iyengar
Running Title: Stochastic spatio-temporal model of cell spreading
Cell Spreading and Focal Adhesion Dynamics Are Regulated by Spacing of Integrin (2007)
Hans-günther Döbereiner, Gregory Giannone, Michael P. Sheetz, E. Fuchs, ...
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ABSTRACT Many cell phenomena involve major morphological changes, particularly in mitosis and the process of cell migration. For cells or neuronal growth cones to migrate, they must extend the...
Yuguang Xiong, Padmini Rangamani, Benjamin Dubin-thaler, Michael P. Sheetz, Ravi Iyengar
Cell motility is important for many physiological processes. The biochemical reactions underlying motility have been well-characterized. Mathematical models, using these biochemical reactions, and...
Position-dependent linkages of fibronectin– integrin–cytoskeleton
Nishizaka, Takayuki, Shi, Qing, Sheetz, Michael P.
Position-dependent cycling of integrin interactions with both the cytoskeleton and extracellular matrix (ECM) is essential for cell spreading, migration, and wound healing. Whether there are regional...
A micromachined device provides a new bend on fibroblast traction forces
Galbraith, Catherine G., Sheetz, Michael P.
We have measured the traction forces generated by fibroblasts using a novel micromachined device that is capable of determining the subcellular forces generated by individual adhesive contacts. The...
Niebuhr, Kirsten, Giuriato, Sylvie, Pedron, Thierry, Philpott, Dana J., Gaits, Frédérique, Sable, Julia, ...
Phosphoinositides play a central role in the control of several cellular events including actin cytoskeleton organization. Here we show that, upon infection of epithelial cells with the Gram-negative...
Single pilus motor forces exceed 100 pN
Maier, Berenike, Potter, Laura, So, Magdalene, Seifert, Hank S., Sheetz, Michael P.
Force production by type IV pilus retraction is critical for infectivity of Neisseria gonorrhoeae and DNA transfer. We investigated the roles of pilus number and the retraction motor, PilT, in force...
De Vos, Kurt J., Sable, Julia, Miller, Kyle E., Sheetz, Michael P.
Axonal transport of membranous organelles such as mitochondria is essential for neuron viability and function. How signaling mechanisms regulate or influence mitochondrial distribution and transport...
Von Wichert, Götz, Haimovich, Beatrice, Feng, Gen-Sheng, Sheetz, Michael P.
As cells encounter new regions of the substrate, they develop bonds with new matrix molecules for migration, matrix remodeling and force generation. How cells orchestrate the assembly of adhesion...
Kwik, Jeanne, Boyle, Sarah, Fooksman, David, Margolis, Leonid, Sheetz, Michael P., Edidin, Michael
Responses to cholesterol depletion are often taken as evidence of a role for lipid rafts in cell function. Here, we show that depletion of cell cholesterol has global effects on cell and plasma...
Modulation of membrane protein lateral mobility by polyphosphates and polyamines
Schindler, Melvin, Koppel, Dennis E., Sheetz, Michael P.
The lateral mobility of fluorescein-labeled membrane glycoproteins was measured in whole unlysed erythrocytes and erythrocyte ghosts by the technique of “fluorescence redistribution after...
Biological Membranes as Bilayer Couples. A Molecular Mechanism of Drug-Erythrocyte Interactions
Sheetz, Michael P., Singer, S. J.
We propose that membranes whose proteins and polar lipids are distributed asymmetrically in the two halves of the membrane bilayer can act as bilayer couples, i.e., the two halves can respond...
A force-dependent switch reverses type IV pilus retraction
Maier, Berenike, Koomey, Michael, Sheetz, Michael P.
Type IV pilus dynamics is important for virulence, motility, and DNA transfer in a wide variety of prokaryotes. The type IV pilus system constitutes a very robust and powerful molecular machine that...
Dubin-Thaler, Benjamin J., Giannone, Gregory, Döbereiner, Hans-Günther, Sheetz, Michael P.
When mouse embryonic fibroblasts in suspension contact a matrix-coated surface, they rapidly adhere and spread. Using total internal reflection fluorescence microscopy of dye-loaded fibroblasts to...
Tension in Tubulovesicular Networks of Golgi and Endoplasmic Reticulum Membranes
Upadhyaya, Arpita, Sheetz, Michael P.
The endoplasmic reticulum (ER) and Golgi have robust bidirectional traffic between them and yet form distinct membrane compartments. Membrane tubules are pulled from large aggregates of ER or Golgi...
Fibronectin Rigidity Response through Fyn and p130Cas Recruitment to the Leading Edge
Kostic, Ana, Sheetz, Michael P.
Cell motility on extracellular matrices critically depends on matrix rigidity, which affects cell adhesion and formation of focal contacts. Receptor-like protein tyrosine phosphatase alpha (RPTPα)...
Position-dependent linkages of fibronectin– integrin–cytoskeleton
Nishizaka, Takayuki, Shi, Qing, Sheetz, Michael P.
Position-dependent cycling of integrin interactions with both the cytoskeleton and extracellular matrix (ECM) is essential for cell spreading, migration, and wound healing. Whether there are regional...
A micromachined device provides a new bend on fibroblast traction forces
Galbraith, Catherine G., Sheetz, Michael P.
We have measured the traction forces generated by fibroblasts using a novel micromachined device that is capable of determining the subcellular forces generated by individual adhesive contacts. The...
Niebuhr, Kirsten, Giuriato, Sylvie, Pedron, Thierry, Philpott, Dana J., Gaits, Frédérique, Sable, Julia, ...
Phosphoinositides play a central role in the control of several cellular events including actin cytoskeleton organization. Here we show that, upon infection of epithelial cells with the Gram-negative...
Single pilus motor forces exceed 100 pN
Maier, Berenike, Potter, Laura, So, Magdalene, Seifert, Hank S., Sheetz, Michael P.
Force production by type IV pilus retraction is critical for infectivity of Neisseria gonorrhoeae and DNA transfer. We investigated the roles of pilus number and the retraction motor, PilT, in force...
De Vos, Kurt J., Sable, Julia, Miller, Kyle E., Sheetz, Michael P.
Axonal transport of membranous organelles such as mitochondria is essential for neuron viability and function. How signaling mechanisms regulate or influence mitochondrial distribution and transport...
Von Wichert, Götz, Haimovich, Beatrice, Feng, Gen-Sheng, Sheetz, Michael P.
As cells encounter new regions of the substrate, they develop bonds with new matrix molecules for migration, matrix remodeling and force generation. How cells orchestrate the assembly of adhesion...
Kwik, Jeanne, Boyle, Sarah, Fooksman, David, Margolis, Leonid, Sheetz, Michael P., Edidin, Michael
Responses to cholesterol depletion are often taken as evidence of a role for lipid rafts in cell function. Here, we show that depletion of cell cholesterol has global effects on cell and plasma...
Modulation of membrane protein lateral mobility by polyphosphates and polyamines
Schindler, Melvin, Koppel, Dennis E., Sheetz, Michael P.
The lateral mobility of fluorescein-labeled membrane glycoproteins was measured in whole unlysed erythrocytes and erythrocyte ghosts by the technique of “fluorescence redistribution after...
Biological Membranes as Bilayer Couples. A Molecular Mechanism of Drug-Erythrocyte Interactions
Sheetz, Michael P., Singer, S. J.
We propose that membranes whose proteins and polar lipids are distributed asymmetrically in the two halves of the membrane bilayer can act as bilayer couples, i.e., the two halves can respond...
A force-dependent switch reverses type IV pilus retraction
Maier, Berenike, Koomey, Michael, Sheetz, Michael P.
Type IV pilus dynamics is important for virulence, motility, and DNA transfer in a wide variety of prokaryotes. The type IV pilus system constitutes a very robust and powerful molecular machine that...
Dubin-Thaler, Benjamin J., Giannone, Gregory, Döbereiner, Hans-Günther, Sheetz, Michael P.
When mouse embryonic fibroblasts in suspension contact a matrix-coated surface, they rapidly adhere and spread. Using total internal reflection fluorescence microscopy of dye-loaded fibroblasts to...
Tension in Tubulovesicular Networks of Golgi and Endoplasmic Reticulum Membranes
Upadhyaya, Arpita, Sheetz, Michael P.
The endoplasmic reticulum (ER) and Golgi have robust bidirectional traffic between them and yet form distinct membrane compartments. Membrane tubules are pulled from large aggregates of ER or Golgi...
Fibronectin Rigidity Response through Fyn and p130Cas Recruitment to the Leading Edge
Kostic, Ana, Sheetz, Michael P.
Cell motility on extracellular matrices critically depends on matrix rigidity, which affects cell adhesion and formation of focal contacts. Receptor-like protein tyrosine phosphatase alpha (RPTPα)...
Rigidity Sensing at the Leading Edge through αvβ3 Integrins and RPTPα
Jiang, Guoying, Huang, Angela H., Cai, Yunfei, Tanase, Monica, Sheetz, Michael P.
Cells require optimal substrate stiffness for normal function and differentiation. The mechanisms for sensing matrix rigidity and durotaxis, however, are not clear. Here we showed that control,...
Nonmuscle Myosin IIA-Dependent Force Inhibits Cell Spreading and Drives F-Actin Flow
Cai, Yunfei, Biais, Nicolas, Giannone, Gregory, Tanase, Monica, Jiang, Guoying, Hofman, Jake M., ...
Nonmuscle myosin IIA (NMM-IIA) is involved in the formation of focal adhesions and neurite retraction. However, the role of NMM-IIA in these functions remains largely unknown. Using RNA interference...
Two distinct modes of myosin assembly and dynamics during epithelial wound closure
Tamada, Masako, Perez, Tomas D., Nelson, W. James, Sheetz, Michael P.
Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin–Darby...
Direct evidence for coherent low velocity axonal transport of mitochondria
Miller, Kyle E., Sheetz, Michael P.
Axonal growth depends on axonal transport. We report the first global analysis of mitochondrial transport during axonal growth and pauses. In the proximal axon, we found that docked mitochondria...
Two distinct modes of myosin assembly and dynamics during epithelial wound closure
Tamada, Masako, Perez, Tomas D., Nelson, W. James, Sheetz, Michael P.
Yauch, Robert L., Felsenfeld, Dan P., Kraeft, Stine-Kathrein, Chen, Lan Bo, Sheetz, Michael P., Hemler, Martin E.
Previous studies have shown that integrin α chain tails make strong positive contributions to integrin-mediated cell adhesion. We now show here that integrin α4 tail deletion markedly impairs...
The Secretion-coupled Endocytosis Correlates with Membrane Tension Changes in RBL 2H3 Cells
Dai, Jianwu, Ting-Beall, H. Ping, Sheetz, Michael P.
Stimulated secretion in endocrine cells and neuronal synapses causes a rise in endocytosis rates to recover the added membrane. The endocytic process involves the mechanical deformation of the...
Membrane Expansion Increases Endocytosis Rate during Mitosis
Raucher, Drazen, Sheetz, Michael P.
Mitosis in mammalian cells is accompanied by a dramatic inhibition of endocytosis. We have found that the addition of amphyphilic compounds to metaphase cells increases the endocytosis rate even to...
Cell Spreading and Lamellipodial Extension Rate Is Regulated by Membrane Tension
Raucher, Drazen, Sheetz, Michael P.
Cell spreading and motility require the extension of the plasma membrane in association with the assembly of actin. In vitro, extension must overcome resistance from tension within the plasma...
Keratocytes Pull with Similar Forces on Their Dorsal and Ventral Surfaces
Galbraith, Catherine G., Sheetz, Michael P.
As cells move forward, they pull rearward against extracellular matrices (ECMs), exerting traction forces. However, no rearward forces have been seen in the fish keratocyte. To address this...
RPTP-α acts as a transducer of mechanical force on αv/β3-integrin–cytoskeleton linkages
Von Wichert, Götz, Jiang, Guoying, Kostic, Ana, De Vos, Kurt, Sap, Jan, Sheetz, Michael P.
Cell motility on ECM critically depends on the cellular response to force from the matrix. We find that force-dependent reinforcement of αv/β3-integrin–mediated cell–matrix connections requires...
The relationship between force and focal complex development
Galbraith, Catherine G., Yamada, Kenneth M., Sheetz, Michael P.
To adhere and migrate, cells must be capable of applying cytoskeletal force to the extracellular matrix (ECM) through integrin receptors. However, it is unclear if connections between integrins and...
Giannone, Grégory, Jiang, Guoying, Sutton, Deborah H., Critchley, David R., Sheetz, Michael P.
Cells rapidly transduce forces exerted on extracellular matrix contacts into tyrosine kinase activation and recruitment of cytoskeletal proteins to reinforce integrin–cytoskeleton connections and...
Force transduction by Triton cytoskeletons
Sawada, Yasuhiro, Sheetz, Michael P.
Force-initiated signal transduction can occur either via membrane-based ionic mechanisms or through changes in cytoskeletal–matrix linkages. We report here the stretch-dependent binding of...
Dubin-Thaler, Benjamin J., Hofman, Jake M., Cai, Yunfei, Xenias, Harry, Spielman, Ingrid, Shneidman, Anna V., ...
Actin-based cell motility and force generation are central to immune response, tissue development, and cancer metastasis, and understanding actin cytoskeleton regulation is a major goal of cell...
Differential Matrix Rigidity Response in Breast Cancer Cell Lines Correlates with the Tissue Tropism
Kostic, Ana, Lynch, Christopher D., Sheetz, Michael P.
Metastasis to a variety of distant organs, such as lung, brain, bone, and liver, is a leading cause of mortality in the breast cancer patients. The tissue tropism of breast cancer metastasis has been...
Plasma Membrane Area Increases with Spread Area by Exocytosis of a GPI-anchored Protein Compartment
Gauthier, Nils C., Rossier, Olivier M., Mathur, Anurag, Hone, James C., Sheetz, Michael P.
The role of plasma membrane (PM) area as a critical factor during cell motility is poorly understood, mainly due to an inability to precisely follow PM area dynamics. To address this fundamental...