Michael P. Washburn

Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes (2009)

Lee, Kenneth K, Swanson, Selene K, Florens, Laurence, Washburn, Michael P, Workman, Jerry L

Abstract Spinocerebellar ataxia (SCA) is a physically devastating, genetically inherited disorder characterized by abnormal brain function that results in the progressive loss of the ability to...

The Inhibition of Polo Kinase by Matrimony Maintains G2 Arrest in the Meiotic Cell Cycle (2007)

Youbin Xiang, Satomi Takeo, Laurence Florens, Stacie E. Hughes, Li-Jun Huo, William D. Gilliland, ...

Many meiotic systems in female animals include a lengthy arrest in G2 that separates the end of pachytene from nuclear envelope breakdown (NEB). However, the mechanisms by which a meiotic cell can...

An ortholog of the Ro autoantigen functions in 23S rRNA maturation in D. radiodurans (2007)

Chen, Xinguo, Wurtmann, Elisabeth J., Van Batavia, Jason, Zybailov, Boris, Washburn, Michael P., Wolin, Sandra L.

In both animal cells and the eubacterium Deinococcus radiodurans, the Ro autoantigen, a ring-shaped RNA-binding protein, associates with small RNAs called Y RNAs. In vertebrates, Ro also binds the...

The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF (2005)

Prochasson, Philippe, Florens, Laurence, Swanson, Selene K., Washburn, Michael P., Workman, Jerry L.

The histone regulatory (HIR) and histone promoter control (HPC) repressor proteins regulate three of the four histone gene loci during the Saccharomyces cerevisiae cell cycle. Here, we demonstrate...

A Mammalian Mediator Subunit that Shares Properties with Saccharomyces cerevisiae Mediator Subunit Cse2 (2004)

Tomomori-Sato, Chieri, Sato, Shigeo, Parmely, Tari J., Banks, Charles A. S., Sorokina, Irina, Florens, Laurence, ...

The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. We previously identified and partially purified a...

Proteomic survey of metabolic pathways in rice

Koller, Antonius, Washburn, Michael P., Lange, B. Markus, Andon, Nancy L., Deciu, Cosmin, Haynes, Paul A., ...

A systematic proteomic analysis of rice (Oryza sativa) leaf, root, and seed tissue using two independent technologies, two-dimensional gel electrophoresis followed by tandem mass spectrometry and...

Protein pathway and complex clustering of correlated mRNA and protein expression analyses in Saccharomyces cerevisiae

Washburn, Michael P., Koller, Antonius, Oshiro, Guy, Ulaszek, Ryan R., Plouffe, David, Deciu, Cosmin, ...

The mRNA and protein expression in Saccharomyces cerevisiae cultured in rich or minimal media was analyzed by oligonucleotide arrays and quantitative multidimensional protein identification...

Parallel Identification of New Genes in Saccharomyces cerevisiae

Oshiro, Guy, Wodicka, Lisa M., Washburn, Michael P., Yates, John R., Lockhart, David J., Winzeler, Elizabeth A.

Short open reading frames (ORFs) occur frequently in primary genome sequence. Distinguishing bona fide small genes from the tens of thousands of short ORFs is one of the most challenging aspects of...

The Deubiquitylation Activity of Ubp8 Is Dependent upon Sgf11 and Its Association with the SAGA Complex

Lee, Kenneth K., Florens, Laurence, Swanson, Selene K., Washburn, Michael P., Workman, Jerry L.

Covalent modifications of the histone tails and the cross talk between these modifications are hallmark features of gene regulation. The SAGA histone acetyltransferase complex is one of the most...

Host Cell Factor and an Uncharacterized SANT Domain Protein Are Stable Components of ATAC, a Novel dAda2A/dGcn5-Containing Histone Acetyltransferase Complex in Drosophila

Guelman, Sebastián, Suganuma, Tamaki, Florens, Laurence, Swanson, Selene K., Kiesecker, Cheri L., Kusch, Thomas, ...

Gcn5 is a conserved histone acetyltransferase (HAT) found in a number of multisubunit complexes from Saccharomyces cerevisiae, mammals, and flies. We previously identified Drosophila melanogaster...

Mnd1/Hop2 Facilitates Dmc1-Dependent Interhomolog Crossover Formation in Meiosis of Budding Yeast†

Henry, Jill M., Camahort, Raymond, Rice, Douglas A., Florens, Laurence, Swanson, Selene K., Washburn, Michael P., ...

During meiosis, each chromosome must pair with its homolog and undergo meiotic crossover recombination in order to segregate properly at the first meiotic division. Recombination in meiosis in...

The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF

Prochasson, Philippe, Florens, Laurence, Swanson, Selene K., Washburn, Michael P., Workman, Jerry L.

The histone regulatory (HIR) and histone promoter control (HPC) repressor proteins regulate three of the four histone gene loci during the Saccharomyces cerevisiae cell cycle. Here, we demonstrate...

Proteomic survey of metabolic pathways in rice

Koller, Antonius, Washburn, Michael P., Lange, B. Markus, Andon, Nancy L., Deciu, Cosmin, Haynes, Paul A., ...

A systematic proteomic analysis of rice (Oryza sativa) leaf, root, and seed tissue using two independent technologies, two-dimensional gel electrophoresis followed by tandem mass spectrometry and...

Protein pathway and complex clustering of correlated mRNA and protein expression analyses in Saccharomyces cerevisiae

Washburn, Michael P., Koller, Antonius, Oshiro, Guy, Ulaszek, Ryan R., Plouffe, David, Deciu, Cosmin, ...

The mRNA and protein expression in Saccharomyces cerevisiae cultured in rich or minimal media was analyzed by oligonucleotide arrays and quantitative multidimensional protein identification...

Parallel Identification of New Genes in Saccharomyces cerevisiae

Oshiro, Guy, Wodicka, Lisa M., Washburn, Michael P., Yates, John R., Lockhart, David J., Winzeler, Elizabeth A.

Short open reading frames (ORFs) occur frequently in primary genome sequence. Distinguishing bona fide small genes from the tens of thousands of short ORFs is one of the most challenging aspects of...

The Deubiquitylation Activity of Ubp8 Is Dependent upon Sgf11 and Its Association with the SAGA Complex

Lee, Kenneth K., Florens, Laurence, Swanson, Selene K., Washburn, Michael P., Workman, Jerry L.

Covalent modifications of the histone tails and the cross talk between these modifications are hallmark features of gene regulation. The SAGA histone acetyltransferase complex is one of the most...

The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF

Prochasson, Philippe, Florens, Laurence, Swanson, Selene K., Washburn, Michael P., Workman, Jerry L.

The histone regulatory (HIR) and histone promoter control (HPC) repressor proteins regulate three of the four histone gene loci during the Saccharomyces cerevisiae cell cycle. Here, we demonstrate...

Host Cell Factor and an Uncharacterized SANT Domain Protein Are Stable Components of ATAC, a Novel dAda2A/dGcn5-Containing Histone Acetyltransferase Complex in Drosophila

Guelman, Sebastián, Suganuma, Tamaki, Florens, Laurence, Swanson, Selene K., Kiesecker, Cheri L., Kusch, Thomas, ...

Gcn5 is a conserved histone acetyltransferase (HAT) found in a number of multisubunit complexes from Saccharomyces cerevisiae, mammals, and flies. We previously identified Drosophila melanogaster...

Mnd1/Hop2 Facilitates Dmc1-Dependent Interhomolog Crossover Formation in Meiosis of Budding Yeast†

Henry, Jill M., Camahort, Raymond, Rice, Douglas A., Florens, Laurence, Swanson, Selene K., Washburn, Michael P., ...

During meiosis, each chromosome must pair with its homolog and undergo meiotic crossover recombination in order to segregate properly at the first meiotic division. Recombination in meiosis in...

The Essential Gene wda Encodes a WD40 Repeat Subunit of Drosophila SAGA Required for Histone H3 Acetylation

Guelman, Sebastián, Suganuma, Tamaki, Florens, Laurence, Weake, Vikki, Swanson, Selene K., Washburn, Michael P., ...

Histone acetylation provides a switch between transcriptionally repressive and permissive chromatin. By regulating the chromatin structure at specific promoters, histone acetyltransferases (HATs)...

Ctk Complex-Mediated Regulation of Histone Methylation by COMPASS▿

Wood, Adam, Shukla, Abhijit, Schneider, Jessica, Lee, Jung Shin, Stanton, Julie D., Dzuiba, Tiffany, ...

A comparative global proteomic screen identified factors required for COMPASS (complex of proteins associated with Set1)-mediated mono-, di-, and trimethylation of the fourth lysine of histone H3...

Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors

Paoletti, Andrew C., Parmely, Tari J., Tomomori-Sato, Chieri, Sato, Shigeo, Zhu, Dongxiao, Conaway, Ronald C., ...

Components of multiprotein complexes are routinely determined by using proteomic approaches. However, this information lacks functional content except when new complex members are identified. To...

An ortholog of the Ro autoantigen functions in 23S rRNA maturation in D. radiodurans

Chen, Xinguo, Wurtmann, Elisabeth J., Van Batavia, Jason, Zybailov, Boris, Washburn, Michael P., Wolin, Sandra L.

In both animal cells and the eubacterium Deinococcus radiodurans, the Ro autoantigen, a ring-shaped RNA-binding protein, associates with small RNAs called Y RNAs. In vertebrates, Ro also binds the...

Lentiviral Vpr usurps Cul4–DDB1[VprBP] E3 ubiquitin ligase to modulate cell cycle

Hrecka, Kasia, Gierszewska, Magdalena, Srivastava, Smita, Kozaczkiewicz, Lukasz, Swanson, Selene K., Florens, Laurence, ...

The replication of viruses depends on the cell cycle status of the infected cells. Viruses have evolved functions that alleviate restrictions imposed on their replication by the host. Vpr, an...

The Inhibition of Polo Kinase by Matrimony Maintains G2 Arrest in the Meiotic Cell Cycle

Xiang, Youbin, Takeo, Satomi, Florens, Laurence, Hughes, Stacie E, Huo, Li-Jun, Gilliland, William D, ...

Many meiotic systems in female animals include a lengthy arrest in G2 that separates the end of pachytene from nuclear envelope breakdown (NEB). However, the mechanisms by which a meiotic cell can...

CHD8 Associates with Human Staf and Contributes to Efficient U6 RNA Polymerase III Transcription▿

Yuan, Chih-Chi, Zhao, Xinyang, Florens, Laurence, Swanson, Selene K., Washburn, Michael P., Hernandez, Nouria

Chromatin remodeling and histone modification are essential for eukaryotic transcription regulation, but little is known about chromatin-modifying activities acting on RNA polymerase III (Pol...

Lentiviral Vpx Accessory Factor Targets VprBP/DCAF1 Substrate Adaptor for Cullin 4 E3 Ubiquitin Ligase to Enable Macrophage Infection

Srivastava, Smita, Swanson, Selene K., Manel, Nicolas, Florens, Laurence, Washburn, Michael P., Skowronski, Jacek

Vpx is a small virion-associated adaptor protein encoded by viruses of the HIV-2/SIVsm lineage of primate lentiviruses that enables these viruses to transduce monocyte-derived cells. This probably...

Probabilistic assembly of human protein interaction networks from label-free quantitative proteomics

Sardiu, Mihaela E., Cai, Yong, Jin, Jingji, Swanson, Selene K., Conaway, Ronald C., Conaway, Joan W., ...

Large-scale affinity purification and mass spectrometry studies have played important roles in the assembly and analysis of comprehensive protein interaction networks for lower eukaryotes. However,...

Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes

Lee, Kenneth K, Swanson, Selene K, Florens, Laurence, Washburn, Michael P, Workman, Jerry L

Spinocerebellar ataxia (SCA) is a physically devastating, genetically inherited disorder characterized by abnormal brain function that results in the progressive loss of the ability to coordinate...

Poly(ADP-ribosyl)ation directs recruitment and activation of an ATP-dependent chromatin remodeler

Gottschalk, Aaron J., Timinszky, Gyula, Kong, Stephanie E., Jin, Jingji, Cai, Yong, Swanson, Selene K., ...

Posttranslational modifications play a key role in recruiting chromatin remodeling and modifying enzymes to specific regions of chromosomes to modulate chromatin structure. Alc1 (amplified in liver...

Determining Protein Complex Connectivity Using a Probabilistic Deletion Network Derived from Quantitative Proteomics

Sardiu, Mihaela E., Gilmore, Joshua M., Carrozza, Michael J., Li, Bing, Workman, Jerry L., Florens, Laurence, ...

Protein complexes are key molecular machines executing a variety of essential cellular processes. Despite the availability of genome-wide protein-protein interaction studies, determining the...