Michel Brahic

The Role of Myelin in Theiler's Virus Persistence in the Central Nervous System (2007)

Jean-Pierre Roussarie, Claude Ruffié, Michel Brahic

Theiler's virus, a picornavirus, persists for life in the central nervous system of mouse and causes a demyelinating disease that is a model for multiple sclerosis. The virus infects neurons first...

An Attenuated Variant of the GDVII Strain of Theiler’s Virus Does Not Persist and Does Not Infect the White Matter of the Central Nervous System

Jarousse, Nadine, Viktorova, Ekaterina G., Pilipenko, Evgeny V., Agol, Vadim I., Brahic, Michel

The DA strain of Theiler’s virus causes a persistent and demyelinating infection of the white matter of spinal cord, whereas the GDVII strain causes a fatal gray-matter encephalomyelitis. Studies...

Distinct Attenuation Phenotypes Caused by Mutations in the Translational Starting Window of Theiler’s Murine Encephalomyelitis Virus

Pilipenko, Evgeny V., Viktorova, Ekaterina G., Khitrina, Elena V., Maslova, Svetlana V., Jarousse, Nadine, Brahic, Michel, ...

Upon initiation of translation of picornavirus RNA, the ribosome is believed to bind the internal ribosome entry site of the template and then to form a productive complex with a downstream RNA...

Theiler’s Virus Infection of Perforin-Deficient Mice

Pena Rossi, Claudia, McAllister, Andrés, Tanguy, Myriam, Kägi, David, Brahic, Michel

Theiler’s virus, a murine picornavirus, infects the central nervous systems of C57BL/6 mice and is cleared after approximately 10 days by a process which requires CD8+ cytotoxic T cells. We used...

The Neurovirulence of the DA and GDVII Strains of Theiler’s Virus Correlates with Their Ability To Infect Cultured Neurons

Jarousse, Nadine, Syan, Sylvie, Martinat, Cécile, Brahic, Michel

The strains of Theiler’s murine encephalomyelitis virus, a picornavirus, are divided into two groups according to their neurovirulence after intracerebral inoculation. The highly virulent GDVII...

Interaction of Theiler’s Virus with Intermediate Filaments of Infected Cells

Nédellec, Patrick, Vicart, Patrick, Laurent-Winter, Christine, Martinat, Cécile, Prévost, Marie-Christine, Brahic, Michel

Theiler’s murine encephalomyelitis virus is a neurotropic murine picornavirus which replicates permissively and causes a cytopathic effect in the BHK-21 cell line. We examined the interactions...

H-2Db−/− Mice Are Susceptible to Persistent Infection by Theiler's Virus

Azoulay-Cayla, Arièle, Dethlefs, Sven, Pérarnau, Béatrice, Larsson-Sciard, Eva-Lotta, Lemonnier, François A., Brahic, Michel, ...

H-2b mice are resistant to persistent infection of the central nervous system by Theiler's virus. They clear the infection 7 to 10 days after intracranial inoculation. Resistance maps to the H-2D...

The GDVII Strain of Theiler’s Virus Spreads via Axonal Transport

Martinat, Cécile, Jarousse, Nadine, Prévost, Marie-Christine, Brahic, Michel

Following intracerebral inoculation, the DA strain of Theiler’s virus sequentially infects neurons in the gray matter and glial cells in the white matter of the spinal cord. It persists in the...

Viral Load and a Locus on Chromosome 11 Affect the Late Clinical Disease Caused by Theiler’s Virus

Aubagnac, Stéphanie, Brahic, Michel, Bureau, Jean-François

Theiler’s virus causes a persistent infection and a demyelinating disease of mice which is a model for multiple sclerosis. Susceptibility to viral persistence maps to several loci, including the...

Viral Load Increases in SJL/J Mice Persistently Infected by Theiler's Virus after Inactivation of the β2m Gene

Aubagnac, Stéphanie, Brahic, Michel, Bureau, Jean-François

We show that inactivating the β2m gene increases the viral load of SJL/J mice persistently infected by Theiler's virus. Together with previous results, this shows that the characteristics of Tmevp1,...

1-β-d-Arabinofuranosylcytosine Inhibits Borna Disease Virus Replication and Spread

Bajramovic, Jeffrey J., Syan, Sylvie, Brahic, Michel, De La Torre, Juan Carlos, Gonzalez-Dunia, Daniel

Borna disease virus (BDV) is a nonsegmented, negative-strand RNA virus that causes neurological diseases in a variety of warm-blooded animal species. There is general consensus that BDV can also...

A Chimeric Human T-Cell Lymphotropic Virus Type 1 with the Envelope Glycoprotein of Moloney Murine Leukemia Virus Is Infectious for Murine Cells

Delebecque, Frédéric, Pramberger, Karin, Prévost, Marie-Christine, Brahic, Michel, Tangy, Frédéric

We constructed a chimeric human T-cell lymphotropic virus type 1 (HTLV-1) provirus in which the original envelope precursor sequence was replaced by that of ecotropic Moloney murine leukemia virus...

Theiler's Virus Infection of Primary Cultures of Bone Marrow-Derived Monocytes/Macrophages

Martinat, Cécile, Mena, Ignacio, Brahic, Michel

Theiler's virus, a murine picornavirus, causes a persistent infection of macrophage/microglial cells in the central nervous systems of SJL/J mice. Viral replication is restricted in the majority of...

Bone Marrow Chimeras Reveal Non-H-2 Hematopoietic Control of Susceptibility to Theiler's Virus Persistent Infection

Aubagnac, Stéphanie, Brahic, Michel, Bureau, Jean-François

The DA strain of Theiler's murine encephalomyelitis virus persists in the white matter of the spinal cords of susceptible mice. Previous results showed that the difference in susceptibility to viral...

Tmevpg1, a Candidate Gene for the Control of Theiler's Virus Persistence, Could Be Implicated in the Regulation of Gamma Interferon

Vigneau, Soline, Rohrlich, Pierre-Simon, Brahic, Michel, Bureau, Jean-François

The Tmevp3 locus controls the load of Theiler's virus RNA during persistent infection of the mouse central nervous system (CNS). We identified a candidate gene at this locus, Tmevpg1, by using a...

A Molecularly Cloned Schwarz Strain of Measles Virus Vaccine Induces Strong Immune Responses in Macaques and Transgenic Mice

Combredet, Chantal, Labrousse, Valérie, Mollet, Lucile, Lorin, Clarisse, Delebecque, Frédéric, Hurtrel, Bruno, ...

Live attenuated RNA viruses make highly efficient vaccines. Among them, measles virus (MV) vaccine has been given to a very large number of children and has been shown to be highly efficacious and...

Borna Disease Virus Glycoprotein Is Required for Viral Dissemination in Neurons

Bajramovic, Jeffrey J., Münter, Sylvia, Syan, Sylvie, Nehrbass, Ulf, Brahic, Michel, Gonzalez-Dunia, Daniel

Borna disease virus (BDV) is a nonsegmented negative-strand RNA virus with a tropism for neurons. Infection with BDV causes neurological diseases in a wide variety of animal species. Although it is...

A Single Injection of Recombinant Measles Virus Vaccines Expressing Human Immunodeficiency Virus (HIV) Type 1 Clade B Envelope Glycoproteins Induces Neutralizing Antibodies and Cellular Immune Responses to HIV

Lorin, Clarisse, Mollet, Lucile, Delebecque, Frédéric, Combredet, Chantal, Hurtrel, Bruno, Charneau, Pierre, ...

The anchored and secreted forms of the human immunodeficiency virus type 1 (HIV-1) 89.6 envelope glycoprotein, either complete or after deletion of the V3 loop, were expressed in a cloned attenuated...

Detection of Tissue Culture-Adapted Theiler's Virus RNA in Spinal Cord White Matter Cells Throughout Infection

Stroop, William G., Brahic, Michel, Baringer, J. Richard

The appearance of histological lesions and the localization of viral RNA in the central nervous system of mice infected with tissue culture-adapted Theiler's murine encephalomyelitis virus (WW...

Infection of Macrophage Primary Cultures by Persistent and Nonpersistent Strains of Theiler's Virus: Role of Capsid and Noncapsid Viral Determinants

Mena, Ignacio, Roussarie, Jean-Pierre, Brahic, Michel

We compared the infection of bone marrow macrophages by the DA and GDVII strains of Theiler's virus and by two viruses constructed by exchanging the DA and GDVII capsids. The replication of the GDVII...

An Attenuated Variant of the GDVII Strain of Theiler’s Virus Does Not Persist and Does Not Infect the White Matter of the Central Nervous System

Jarousse, Nadine, Viktorova, Ekaterina G., Pilipenko, Evgeny V., Agol, Vadim I., Brahic, Michel

The DA strain of Theiler’s virus causes a persistent and demyelinating infection of the white matter of spinal cord, whereas the GDVII strain causes a fatal gray-matter encephalomyelitis. Studies...

Distinct Attenuation Phenotypes Caused by Mutations in the Translational Starting Window of Theiler’s Murine Encephalomyelitis Virus

Pilipenko, Evgeny V., Viktorova, Ekaterina G., Khitrina, Elena V., Maslova, Svetlana V., Jarousse, Nadine, Brahic, Michel, ...

Upon initiation of translation of picornavirus RNA, the ribosome is believed to bind the internal ribosome entry site of the template and then to form a productive complex with a downstream RNA...

Theiler’s Virus Infection of Perforin-Deficient Mice

Pena Rossi, Claudia, McAllister, Andrés, Tanguy, Myriam, Kägi, David, Brahic, Michel

Theiler’s virus, a murine picornavirus, infects the central nervous systems of C57BL/6 mice and is cleared after approximately 10 days by a process which requires CD8+ cytotoxic T cells. We used...

The Neurovirulence of the DA and GDVII Strains of Theiler’s Virus Correlates with Their Ability To Infect Cultured Neurons

Jarousse, Nadine, Syan, Sylvie, Martinat, Cécile, Brahic, Michel

The strains of Theiler’s murine encephalomyelitis virus, a picornavirus, are divided into two groups according to their neurovirulence after intracerebral inoculation. The highly virulent GDVII...

Interaction of Theiler’s Virus with Intermediate Filaments of Infected Cells

Nédellec, Patrick, Vicart, Patrick, Laurent-Winter, Christine, Martinat, Cécile, Prévost, Marie-Christine, Brahic, Michel

Theiler’s murine encephalomyelitis virus is a neurotropic murine picornavirus which replicates permissively and causes a cytopathic effect in the BHK-21 cell line. We examined the interactions...

H-2Db−/− Mice Are Susceptible to Persistent Infection by Theiler's Virus

Azoulay-Cayla, Arièle, Dethlefs, Sven, Pérarnau, Béatrice, Larsson-Sciard, Eva-Lotta, Lemonnier, François A., Brahic, Michel, ...

H-2b mice are resistant to persistent infection of the central nervous system by Theiler's virus. They clear the infection 7 to 10 days after intracranial inoculation. Resistance maps to the H-2D...

The GDVII Strain of Theiler’s Virus Spreads via Axonal Transport

Martinat, Cécile, Jarousse, Nadine, Prévost, Marie-Christine, Brahic, Michel

Following intracerebral inoculation, the DA strain of Theiler’s virus sequentially infects neurons in the gray matter and glial cells in the white matter of the spinal cord. It persists in the...

Viral Load and a Locus on Chromosome 11 Affect the Late Clinical Disease Caused by Theiler’s Virus

Aubagnac, Stéphanie, Brahic, Michel, Bureau, Jean-François

Theiler’s virus causes a persistent infection and a demyelinating disease of mice which is a model for multiple sclerosis. Susceptibility to viral persistence maps to several loci, including the...

Viral Load Increases in SJL/J Mice Persistently Infected by Theiler's Virus after Inactivation of the β2m Gene

Aubagnac, Stéphanie, Brahic, Michel, Bureau, Jean-François

We show that inactivating the β2m gene increases the viral load of SJL/J mice persistently infected by Theiler's virus. Together with previous results, this shows that the characteristics of Tmevp1,...

1-β-d-Arabinofuranosylcytosine Inhibits Borna Disease Virus Replication and Spread

Bajramovic, Jeffrey J., Syan, Sylvie, Brahic, Michel, De La Torre, Juan Carlos, Gonzalez-Dunia, Daniel

Borna disease virus (BDV) is a nonsegmented, negative-strand RNA virus that causes neurological diseases in a variety of warm-blooded animal species. There is general consensus that BDV can also...

A Chimeric Human T-Cell Lymphotropic Virus Type 1 with the Envelope Glycoprotein of Moloney Murine Leukemia Virus Is Infectious for Murine Cells

Delebecque, Frédéric, Pramberger, Karin, Prévost, Marie-Christine, Brahic, Michel, Tangy, Frédéric

We constructed a chimeric human T-cell lymphotropic virus type 1 (HTLV-1) provirus in which the original envelope precursor sequence was replaced by that of ecotropic Moloney murine leukemia virus...

Theiler's Virus Infection of Primary Cultures of Bone Marrow-Derived Monocytes/Macrophages

Martinat, Cécile, Mena, Ignacio, Brahic, Michel

Theiler's virus, a murine picornavirus, causes a persistent infection of macrophage/microglial cells in the central nervous systems of SJL/J mice. Viral replication is restricted in the majority of...

Bone Marrow Chimeras Reveal Non-H-2 Hematopoietic Control of Susceptibility to Theiler's Virus Persistent Infection

Aubagnac, Stéphanie, Brahic, Michel, Bureau, Jean-François

The DA strain of Theiler's murine encephalomyelitis virus persists in the white matter of the spinal cords of susceptible mice. Previous results showed that the difference in susceptibility to viral...

Tmevpg1, a Candidate Gene for the Control of Theiler's Virus Persistence, Could Be Implicated in the Regulation of Gamma Interferon

Vigneau, Soline, Rohrlich, Pierre-Simon, Brahic, Michel, Bureau, Jean-François

The Tmevp3 locus controls the load of Theiler's virus RNA during persistent infection of the mouse central nervous system (CNS). We identified a candidate gene at this locus, Tmevpg1, by using a...

A Molecularly Cloned Schwarz Strain of Measles Virus Vaccine Induces Strong Immune Responses in Macaques and Transgenic Mice

Combredet, Chantal, Labrousse, Valérie, Mollet, Lucile, Lorin, Clarisse, Delebecque, Frédéric, Hurtrel, Bruno, ...

Live attenuated RNA viruses make highly efficient vaccines. Among them, measles virus (MV) vaccine has been given to a very large number of children and has been shown to be highly efficacious and...

Borna Disease Virus Glycoprotein Is Required for Viral Dissemination in Neurons

Bajramovic, Jeffrey J., Münter, Sylvia, Syan, Sylvie, Nehrbass, Ulf, Brahic, Michel, Gonzalez-Dunia, Daniel

Borna disease virus (BDV) is a nonsegmented negative-strand RNA virus with a tropism for neurons. Infection with BDV causes neurological diseases in a wide variety of animal species. Although it is...

A Single Injection of Recombinant Measles Virus Vaccines Expressing Human Immunodeficiency Virus (HIV) Type 1 Clade B Envelope Glycoproteins Induces Neutralizing Antibodies and Cellular Immune Responses to HIV

Lorin, Clarisse, Mollet, Lucile, Delebecque, Frédéric, Combredet, Chantal, Hurtrel, Bruno, Charneau, Pierre, ...

The anchored and secreted forms of the human immunodeficiency virus type 1 (HIV-1) 89.6 envelope glycoprotein, either complete or after deletion of the V3 loop, were expressed in a cloned attenuated...

Detection of Tissue Culture-Adapted Theiler's Virus RNA in Spinal Cord White Matter Cells Throughout Infection

Stroop, William G., Brahic, Michel, Baringer, J. Richard

The appearance of histological lesions and the localization of viral RNA in the central nervous system of mice infected with tissue culture-adapted Theiler's murine encephalomyelitis virus (WW...

Infection of Macrophage Primary Cultures by Persistent and Nonpersistent Strains of Theiler's Virus: Role of Capsid and Noncapsid Viral Determinants

Mena, Ignacio, Roussarie, Jean-Pierre, Brahic, Michel

We compared the infection of bone marrow macrophages by the DA and GDVII strains of Theiler's virus and by two viruses constructed by exchanging the DA and GDVII capsids. The replication of the GDVII...

The Role of Myelin in Theiler's Virus Persistence in the Central Nervous System

Roussarie, Jean-Pierre, Ruffié, Claude, Brahic, Michel

Theiler's virus, a picornavirus, persists for life in the central nervous system of mouse and causes a demyelinating disease that is a model for multiple sclerosis. The virus infects neurons first...

Axon Myelin Transfer of a Non-Enveloped Virus

Roussarie, Jean-Pierre, Ruffié, Claude, Edgar, Julia M., Griffiths, Ian, Brahic, Michel

We showed previously that Theiler's virus, a neurotropic non-enveloped picornavirus of mouse, traffics from the axon of infected neurons into the surrounding myelin. When this traffic is interrupted,...