Michel Frenette

Evolutionary relationships among salivarius streptococci as inferred from multilocus phylogenies based on 16S rRNA-encoding, recA, secA, and secY gene sequences (2009)

Pombert, Jean-François, Sistek, Viridiana, Boissinot, Maurice, Frenette, Michel

Abstract Background Streptococci are divided into six phylogenetic groups, i.e , anginosus, bovis, mitis, mutans, pyogenic, and salivarius, with the salivarius group consisting of only three distinct...

Phenotypic Consequences Resulting from a Methionine-to-Valine Substitution at Position 48 in the HPr Protein of Streptococcus salivarius

Plamondon, Pascale, Brochu, Denis, Thomas, Suzanne, Fradette, Julie, Gauthier, Lucie, Vaillancourt, Katy, ...

In gram-positive bacteria, the HPr protein of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) can be phosphorylated on a histidine residue at position 15 (His15) by enzyme I (EI) of the...

Streptococcus salivarius Fimbriae Are Composed of a Glycoprotein Containing a Repeated Motif Assembled into a Filamentous Nondissociable Structure

Lévesque, Céline, Vadeboncoeur, Christian, Chandad, Fatiha, Frenette, Michel

Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S....

An Effective Strategy, Applicable to Streptococcus salivarius and Related Bacteria, To Enhance or Confer Electroporation Competence

Buckley, Nicole D., Vadeboncoeur, Christian, LeBlanc, Donald J., Lee, Linda N., Frenette, Michel

Despite the large number of techniques available for transformation of bacteria, certain species and strains are still resistant to introduction of foreign DNA. Some oral streptococci are among the...

Genetic and Biochemical Characterization of the Phosphoenolpyruvate:Glucose/Mannose Phosphotransferase System of Streptococcus thermophilus

Cochu, Armelle, Vadeboncoeur, Christian, Moineau, Sylvain, Frenette, Michel

In most streptococci, glucose is transported by the phosphoenolpyruvate (PEP):glucose/mannose phosphotransferase system (PTS) via HPr and IIABMan, two proteins involved in regulatory mechanisms....

Phosphorylation of Streptococcus salivarius Lactose Permease (LacS) by HPr(His∼P) and HPr(Ser-P)(His∼P) and Effects on Growth

Lessard, Christian, Cochu, Armelle, Lemay, Jean-Dominique, Roy, Denis, Vaillancourt, Katy, Frenette, Michel, ...

The oral bacterium Streptococcus salivarius takes up lactose via a transporter called LacS that shares 95% identity with the LacS from Streptococcus thermophilus, a phylogenetically closely related...

Characterization of a Galactokinase-Positive Recombinant Strain of Streptococcus thermophilus

Vaillancourt, Katy, LeMay, Jean-Dominique, Lamoureux, Maryse, Frenette, Michel, Moineau, Sylvain, Vadeboncoeur, Christian

The lactic acid bacterium Streptococcus thermophilus is widely used by the dairy industry for its ability to transform lactose, the primary sugar found in milk, into lactic acid. Unlike the...

The Doubly Phosphorylated Form of HPr, HPr(Ser-P)(His∼P), Is Abundant in Exponentially Growing Cells of Streptococcus thermophilus and Phosphorylates the Lactose Transporter LacS as Efficiently as HPr(His∼P)

Cochu, Armelle, Roy, Denis, Vaillancourt, Katy, LeMay, Jean-Dominique, Casabon, Israël, Frenette, Michel, ...

In Streptococcus thermophilus, lactose is taken up by LacS, a transporter that comprises a membrane translocator domain and a hydrophilic regulatory domain homologous to the IIA proteins and protein...

Characterization of the cro-ori Region of the Streptococcus thermophilus Virulent Bacteriophage DT1

Lamothe, Geneviève, Lévesque, Céline, Bissonnette, Frédéric, Cochu, Armelle, Vadeboncoeur, Christian, Frenette, Michel, ...

The virulent cos-type Streptococcus thermophilus phage DT1 was previously isolated from a mozzarella whey sample, and its complete genomic sequence is available. The putative ori of phage DT1 is...

Phenotypic Consequences Resulting from a Methionine-to-Valine Substitution at Position 48 in the HPr Protein of Streptococcus salivarius

Plamondon, Pascale, Brochu, Denis, Thomas, Suzanne, Fradette, Julie, Gauthier, Lucie, Vaillancourt, Katy, ...

In gram-positive bacteria, the HPr protein of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) can be phosphorylated on a histidine residue at position 15 (His15) by enzyme I (EI) of the...

Streptococcus salivarius Fimbriae Are Composed of a Glycoprotein Containing a Repeated Motif Assembled into a Filamentous Nondissociable Structure

Lévesque, Céline, Vadeboncoeur, Christian, Chandad, Fatiha, Frenette, Michel

Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S....

An Effective Strategy, Applicable to Streptococcus salivarius and Related Bacteria, To Enhance or Confer Electroporation Competence

Buckley, Nicole D., Vadeboncoeur, Christian, LeBlanc, Donald J., Lee, Linda N., Frenette, Michel

Despite the large number of techniques available for transformation of bacteria, certain species and strains are still resistant to introduction of foreign DNA. Some oral streptococci are among the...

Genetic and Biochemical Characterization of the Phosphoenolpyruvate:Glucose/Mannose Phosphotransferase System of Streptococcus thermophilus

Cochu, Armelle, Vadeboncoeur, Christian, Moineau, Sylvain, Frenette, Michel

In most streptococci, glucose is transported by the phosphoenolpyruvate (PEP):glucose/mannose phosphotransferase system (PTS) via HPr and IIABMan, two proteins involved in regulatory mechanisms....

Phosphorylation of Streptococcus salivarius Lactose Permease (LacS) by HPr(His∼P) and HPr(Ser-P)(His∼P) and Effects on Growth

Lessard, Christian, Cochu, Armelle, Lemay, Jean-Dominique, Roy, Denis, Vaillancourt, Katy, Frenette, Michel, ...

The oral bacterium Streptococcus salivarius takes up lactose via a transporter called LacS that shares 95% identity with the LacS from Streptococcus thermophilus, a phylogenetically closely related...

Characterization of a Galactokinase-Positive Recombinant Strain of Streptococcus thermophilus

Vaillancourt, Katy, LeMay, Jean-Dominique, Lamoureux, Maryse, Frenette, Michel, Moineau, Sylvain, Vadeboncoeur, Christian

The lactic acid bacterium Streptococcus thermophilus is widely used by the dairy industry for its ability to transform lactose, the primary sugar found in milk, into lactic acid. Unlike the...

The Doubly Phosphorylated Form of HPr, HPr(Ser-P)(His∼P), Is Abundant in Exponentially Growing Cells of Streptococcus thermophilus and Phosphorylates the Lactose Transporter LacS as Efficiently as HPr(His∼P)

Cochu, Armelle, Roy, Denis, Vaillancourt, Katy, LeMay, Jean-Dominique, Casabon, Israël, Frenette, Michel, ...

In Streptococcus thermophilus, lactose is taken up by LacS, a transporter that comprises a membrane translocator domain and a hydrophilic regulatory domain homologous to the IIA proteins and protein...

Characterization of the cro-ori Region of the Streptococcus thermophilus Virulent Bacteriophage DT1

Lamothe, Geneviève, Lévesque, Céline, Bissonnette, Frédéric, Cochu, Armelle, Vadeboncoeur, Christian, Frenette, Michel, ...

The virulent cos-type Streptococcus thermophilus phage DT1 was previously isolated from a mozzarella whey sample, and its complete genomic sequence is available. The putative ori of phage DT1 is...

Role of galK and galM in Galactose Metabolism by Streptococcus thermophilus▿

Vaillancourt, Katy, Bédard, Nathalie, Bart, Christian, Tessier, Mélanie, Robitaille, Gilles, Turgeon, Nathalie, ...

Streptococcus thermophilus is unable to metabolize the galactose moiety of lactose. In this paper, we show that a transformant of S. thermophilus SMQ-301 expressing Streptococcus salivarius galK and...