The Genome of the Natural Genetic Engineer Agrobacterium (2009)
Tumefaciens C, Derek W. Wood, Joao C. Setubal, Rajinder Kaul, Dave E. Monks, Joao P. Kitajima, ...
The 5.67-megabase genome of the plant pathogen Agrobacterium tumefaciens C58 consists of a circular chromosome, a linear chromosome, and two plasmids. Extensive orthology and nucleotide colinearity...
Tumefuciens Ti Plasmid, John E. Ward, Donna E. Akiyoshis, Dean Regierg, Asis Dattaq, Milton P. Gordon, ...
Ti plasmid are grouped into six transcription tions in these loci result in a reduced ability of Agrobacterium units and direct the transfer of T-DNA into plant cells. to attach to plant cells and an...
Studies on spin labeled ribonucleic acids encapsulated by viral proteins (1978)
Powell, Ann Thomas, Gordon, Milton P., Caspary, William J., Greene, James J., Ts'o, Paul O.P.
Spin labeled poly rA (sl-poly rA) was encapsulated by the coat proteins of two plant viruses having different morphologies: TMV, a rigid rod and CCMV, an icosahedral sphere. Electron microscopy...
Doty, Sharon Lafferty, Shang, Tanya Q., Wilson, Angela M., Tangen, Jeff, Westergreen, Aram D., Newman, Lee A., ...
Chlorinated solvents, especially trichloroethylene (TCE), are the most widespread groundwater contaminants in the United States. Existing methods of pumping and treating are expensive and laborious....
Agrobacterium VirD2 protein interacts with plant host cyclophilins
Deng, Wanyin, Chen, Lishan, Wood, Derek W., Metcalfe, Tracee, Liang, Xiaoyou, Gordon, Milton P., ...
Agrobacterium tumefaciens induces crown gall tumors on plants by transferring a nucleoprotein complex, the T-complex, from the bacterium to the plant cell. The T-complex consists of T-DNA, a...
Eden, Francine C., Farrand, Stephen K., Powell, Jerry S., Bendich, Arnold J., Chilton, Mary-Dell, Nester, Eugene W., ...
Labeled ribonucleic acid (RNA) complementary to Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA (cRNA) were used in a systematic study of the sensitivity of cRNA/deoxyribonucleic acid...
Setlow, Jane K., Brown, Dianne C., Boling, Maxon E., Mattingly, Alice, Gordon, Milton P.
Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with...
Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome
White, Frank F., Ghidossi, Gina, Gordon, Milton P., Nester, Eugene W.
The DNA from tumors of Nicotiana glauca initiated by strains of Agrobacterium rhizogenes was shown to contain sequences that are homologous to the root-inducing (Ri) plasmid of the bacterium. Two...
Proteins encoded by Agrobacterium tumefaciens Ti plasmid DNA (T-DNA) in crown gall tumors
McPherson, Joan C., Nester, Eugene W., Gordon, Milton P.
In order to detect proteins that may be produced in crown gall tumors as a result of expression of incorporated Agrobacterium tumefaciens Ti plasmid DNA (T-DNA), we have isolated mRNA complementary...
T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells
Chilton, Mary-Dell, Saiki, Randall K., Yadav, Narendra, Gordon, Milton P., Quetier, Francis
The crown gall teratoma tumor line BT37, incited by Agrobacterium tumefaciens strain T37, has been found to contain part of the tumor-inducing plasmid, pTi T37, of the inciting strain. This foreign...
Detection and Quantitation of Octopine in Normal Plant Tissue and in Crown Gall Tumors
Johnson, Roosevelt, Guderian, Ronald H., Eden, Francine, Chilton, Mary-Dell, Gordon, Milton P., Nester, Eugene W.
Octopine has been detected in normal tobacco leaf and stem tissue, normal sunflower stem tissue, pinto bean leaves, and normal tobacco callus tissue in culture. Octopine was identified in extracts by...
Studies on Spinach Chloroplast and Nuclear DNA Using Large-scale Tissue Preparations 1
Bard, Stephen A., Gordon, Milton P.
A method for the large-scale preparation of spinach chloroplasts using the Spinco Model L-4 zonal ultracentrifuge and for the extraction of DNA from the chloroplasts is described. Thirty-five percent...
Agrobacterium tumefaciens DNA and PS8 Bacteriophage DNA Not Detected in Crown Gall Tumors
Chilton, Mary-Dell, Currier, Thomas C., Farrand, Stephen K., Bendich, Arnold J., Gordon, Milton P., Nester, Eugene W.
Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 104-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added...
Sinkar, Vilas P., White, Frank F., Furner, Ian J., Abrahamsen, Mitchell, Pythoud, Francois, Gordon, Milton P.
Transgenic plants harboring the left transfer DNA (TL-DNA) of the root inducing plasmid of Agrobacterium rhizogenes show many developmental abnormalities. We observed frequent appearance of normal...
Doty, Sharon Lafferty, Shang, Tanya Q., Wilson, Angela M., Tangen, Jeff, Westergreen, Aram D., Newman, Lee A., ...
Chlorinated solvents, especially trichloroethylene (TCE), are the most widespread groundwater contaminants in the United States. Existing methods of pumping and treating are expensive and laborious....
Agrobacterium VirD2 protein interacts with plant host cyclophilins
Deng, Wanyin, Chen, Lishan, Wood, Derek W., Metcalfe, Tracee, Liang, Xiaoyou, Gordon, Milton P., ...
Agrobacterium tumefaciens induces crown gall tumors on plants by transferring a nucleoprotein complex, the T-complex, from the bacterium to the plant cell. The T-complex consists of T-DNA, a...
Eden, Francine C., Farrand, Stephen K., Powell, Jerry S., Bendich, Arnold J., Chilton, Mary-Dell, Nester, Eugene W., ...
Labeled ribonucleic acid (RNA) complementary to Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA (cRNA) were used in a systematic study of the sensitivity of cRNA/deoxyribonucleic acid...
Setlow, Jane K., Brown, Dianne C., Boling, Maxon E., Mattingly, Alice, Gordon, Milton P.
Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with...
Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome
White, Frank F., Ghidossi, Gina, Gordon, Milton P., Nester, Eugene W.
The DNA from tumors of Nicotiana glauca initiated by strains of Agrobacterium rhizogenes was shown to contain sequences that are homologous to the root-inducing (Ri) plasmid of the bacterium. Two...
Proteins encoded by Agrobacterium tumefaciens Ti plasmid DNA (T-DNA) in crown gall tumors
McPherson, Joan C., Nester, Eugene W., Gordon, Milton P.
In order to detect proteins that may be produced in crown gall tumors as a result of expression of incorporated Agrobacterium tumefaciens Ti plasmid DNA (T-DNA), we have isolated mRNA complementary...
T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells
Chilton, Mary-Dell, Saiki, Randall K., Yadav, Narendra, Gordon, Milton P., Quetier, Francis
The crown gall teratoma tumor line BT37, incited by Agrobacterium tumefaciens strain T37, has been found to contain part of the tumor-inducing plasmid, pTi T37, of the inciting strain. This foreign...
Detection and Quantitation of Octopine in Normal Plant Tissue and in Crown Gall Tumors
Johnson, Roosevelt, Guderian, Ronald H., Eden, Francine, Chilton, Mary-Dell, Gordon, Milton P., Nester, Eugene W.
Octopine has been detected in normal tobacco leaf and stem tissue, normal sunflower stem tissue, pinto bean leaves, and normal tobacco callus tissue in culture. Octopine was identified in extracts by...
Studies on Spinach Chloroplast and Nuclear DNA Using Large-scale Tissue Preparations 1
Bard, Stephen A., Gordon, Milton P.
A method for the large-scale preparation of spinach chloroplasts using the Spinco Model L-4 zonal ultracentrifuge and for the extraction of DNA from the chloroplasts is described. Thirty-five percent...
Agrobacterium tumefaciens DNA and PS8 Bacteriophage DNA Not Detected in Crown Gall Tumors
Chilton, Mary-Dell, Currier, Thomas C., Farrand, Stephen K., Bendich, Arnold J., Gordon, Milton P., Nester, Eugene W.
Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 104-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added...
Sinkar, Vilas P., White, Frank F., Furner, Ian J., Abrahamsen, Mitchell, Pythoud, Francois, Gordon, Milton P.
Transgenic plants harboring the left transfer DNA (TL-DNA) of the root inducing plasmid of Agrobacterium rhizogenes show many developmental abnormalities. We observed frequent appearance of normal...