Reshuffling of the Bacillus subtilis 168 Genome by Multifold Inversion (2008)
Kuroki, Azusa, Toda, Tsutomu, Matsui, Kuniko, Uotsu-Tomita, Rie, Tomita, Masaru, Itaya, Mitsuhiro
The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires...
Morohashi, Mineo, Ohashi, Yoshiaki, Tani, Saeka, Ishii, Kotaro, Itaya, Mitsuhiro, Nanamiya, Hideaki, ...
The soil bacterium Bacillus subtilis forms dormant, robust spores as a tactic to ensure survival under conditions of starvation. However, the sporulating culture includes sporulating and...
Reassessment of the In Vivo Functions of DNA Polymerase I and (2007)
Sanae Fukushima, Mitsuhiro Itaya, Hiroaki Kato, Naotake Ogasawara, Hirofumi Yoshikawa
A major factor in removing RNA primers during the processing of Okazaki fragments is DNA polymerase I (Pol I). Pol I is thought to remove the RNA primers and to fill the resulting gaps...
Sakaya, Nagayoshi, Kaneko, Shinya, Matsunaga, Satoko, Itaya, Mitsuhiro
The use of Bacillus subtilis 168 as the initial host for molecular cloning and subsequent delivery of the engineered DNA to other Bacillus hosts appears attractive, and would lead to an efficient DNA...
Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro
ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein exhibited RNase H activity in both in vivo and...
Construction of physical maps of Bacillus subtilis (natto) strains (2003)
Qiu, Dongru, Oshima, Hideyuki, Ohashi, Yoshiaki, Itaya, Mitsuhiro
The putative physical map of a Bacillus subtilis (natto) strain has been constructed to demonstrate the difference from the strain B. subtilis Marburg. B. subtilis (natto) strains are closely related...
Nishimura, Seiichiro, Tanaka, Terumichi, Fujita, Kyoko, Itaya, Mitsuhiro, Hiraishi, Akira, Kikuchi, Yo
A marine photosynthetic bacterium Rhodovulum sulfidophilum secretes nucleic acids that are involved in flocculating ability. These extracellular nucleic acids have not been well characterized. Here,...
Application of recombination transfer to the cognate Bacillus subtilis genome (2003)
Tomita, Satoshi, Tsuge, Kenji, Kikuchi, Yo, Itaya, Mitsuhiro
Isolation of the designated genome region of Bacillus subtilis was investigated using a B. subtilis recombinational transfer (BReT) system. Two DNA sequences flanking the precise genome region are...
Stable Positional Cloning of Long Continuous DNA in the Bacillus subtilis Genome Vector (2003)
Itaya, Mitsuhiro, Fujita, Kyoko, Ikeuchi, Masahiko, Koizumi, Maki, Tsuge, Kenji
Direct cloning of a long continuous genome segment in a Bacillus subtilis genome vector was demonstrated for the first time. Two small DNA fragments had to be installed in the vector prior to...
Kaneko, Shinya, Tsuge, Kenji, Takeuchi, Takashi, Itaya, Mitsuhiro
A novel genome vector using the 4215 kb Bacillus subtilis genome provides for precise target cloning and processing of the cloned DNA to the desired structure. Each process highly dependent on...
Tsuge, Kenji, Matsui, Kuniko, Itaya, Mitsuhiro
A universal method to reconstitute sets of genes was developed. Owing to the intrinsic nature of the plasmid establishment mechanism in Bacillus subtilis, the assembly of five antibiotic resistance...
Construction of physical maps of Bacillus subtilis (natto) strains (2003)
Qiu, Dongru, Oshima, Hideyuki, Ohashi, Yoshiaki, Itaya, Mitsuhiro
Nishimura, Seiichiro, Tanaka, Terumichi, Fujita, Kyoko, Itaya, Mitsuhiro, Hiraishi, Akira, Kikuchi, Yo
Application of recombination transfer to the cognate Bacillus subtilis genome (2003)
Tomita, Satoshi, Tsuge, Kenji, Kikuchi, Yo, Itaya, Mitsuhiro
Itaya, Mitsuhiro, Kondo, Kanae
A DNA fragment encoding Ribonuclease H (EC 3. 1. 26. 4) was isolated from an extreme thermophilic bacterium, Thermus thermophilus HB8, by its ability to complement the temperature-sensitive growth of...
Itaya, Mitsuhiro, Inoue, Yasuo
A series of alkyl esters of guanosine 3′-phosphate has been synthesized, and the Michaelis-Menten parameters for the RNase T1-catalyzed transphosphorylation of these substrates have been determined...
Experimental surgery to create subgenomes of Bacillus subtilis 168
Itaya, Mitsuhiro, Tanaka, Teruo
The 4,188-kb circular genome of Bacillus subtilis 168 was artificially dissected into two stable circular chromosomes in vivo, one being the 3,878-kb main genome and the other the 310-kb subgenome...
Genetic Transfer of Large DNA Inserts to Designated Loci of the Bacillus subtilis 168 Genome
It was found that contiguous DNA segments of up to 50 kb can be transferred between Bacillus subtilis genomes when a sufficient length of the flanking genomic region is provided for homologous...
Isolation of RNase H Genes That Are Essential for Growth of Bacillus subtilis 168
Itaya, Mitsuhiro, Omori, Akira, Kanaya, Shigenori, Crouch, Robert J., Tanaka, Teruo, Kondo, Kanae
Two genes encoding functional RNase H (EC 3.1.26.4) were isolated from a gram-positive bacterium, Bacillus subtilis 168. Two DNA clones exhibiting RNase H activities both in vivo and in vitro were...
Recombinational Transfer of 100-Kilobase Genomic DNA to Plasmid in Bacillus subtilis 168
Tsuge, Kenji, Itaya, Mitsuhiro
Transformation of Bacillus subtilis by a plasmid requires a circular multimeric form. In contrast, linearized plasmids can be circularized only when homologous sequences are present in the host...
Matsumoto, Kouji, Okada, Masahiro, Horikoshi, Yuko, Matsuzaki, Hiroshi, Kishi, Tsutomu, Itaya, Mitsuhiro, ...
The psd gene of Bacillus subtilis Marburg, encoding phosphatidylserine decarboxylase, has been cloned and sequenced. It encodes a polypeptide of 263 amino acid residues (deduced molecular weight of...
Conversion of sub-megasized DNA to desired structures using a novel Bacillus subtilis genome vector
Kaneko, Shinya, Tsuge, Kenji, Takeuchi, Takashi, Itaya, Mitsuhiro
A novel genome vector using the 4215 kb Bacillus subtilis genome provides for precise target cloning and processing of the cloned DNA to the desired structure. Each process highly dependent on...
Tsuge, Kenji, Matsui, Kuniko, Itaya, Mitsuhiro
A universal method to reconstitute sets of genes was developed. Owing to the intrinsic nature of the plasmid establishment mechanism in Bacillus subtilis, the assembly of five antibiotic resistance...
Tomita, Satoshi, Tsuge, Kenji, Kikuchi, Yo, Itaya, Mitsuhiro
A method for positional cloning of the Bacillus subtilis genome was developed. The method requires a set of two small DNA fragments that flank the region to be copied. A 38-kb segment that carries...
Comparative Analysis of Physical Maps of Four Bacillus subtilis (natto) Genomes
Qiu, Dongru, Fujita, Kyoko, Sakuma, Yuko, Tanaka, Teruo, Ohashi, Yoshiaki, Ohshima, Hideyuki, ...
The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome...
Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7
Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro
ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein exhibited RNase H activity in both in vivo and...
Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro
All the archaeal genomes sequenced to date contain a single Type 2 RNase H gene. We found that the genome of a halophilic archaeon, Halobacterium sp. NRC-1, contains an open reading frame with...
Tsuge, Kenji, Inoue, Satoka, Ano, Takashi, Itaya, Mitsuhiro, Shoda, Makoto
Iturin A and its derivatives are lipopeptide antibiotics produced by Bacillus subtilis and several closely related bacteria. Three iturin group operons (i.e., iturin A, mycosubtilin, and bacillomycin...
Itaya, Mitsuhiro, Tsuge, Kenji, Koizumi, Maki, Fujita, Kyoko
Cloning the whole 3.5-megabase (Mb) genome of the photosynthetic bacterium Synechocystis PCC6803 into the 4.2-Mb genome of the mesophilic bacterium Bacillus subtilis 168 resulted in a 7.7-Mb...
Experimental surgery to create subgenomes of Bacillus subtilis 168
Itaya, Mitsuhiro, Tanaka, Teruo
The 4,188-kb circular genome of Bacillus subtilis 168 was artificially dissected into two stable circular chromosomes in vivo, one being the 3,878-kb main genome and the other the 310-kb subgenome...
Genetic Transfer of Large DNA Inserts to Designated Loci of the Bacillus subtilis 168 Genome
It was found that contiguous DNA segments of up to 50 kb can be transferred between Bacillus subtilis genomes when a sufficient length of the flanking genomic region is provided for homologous...
Isolation of RNase H Genes That Are Essential for Growth of Bacillus subtilis 168
Itaya, Mitsuhiro, Omori, Akira, Kanaya, Shigenori, Crouch, Robert J., Tanaka, Teruo, Kondo, Kanae
Two genes encoding functional RNase H (EC 3.1.26.4) were isolated from a gram-positive bacterium, Bacillus subtilis 168. Two DNA clones exhibiting RNase H activities both in vivo and in vitro were...
Recombinational Transfer of 100-Kilobase Genomic DNA to Plasmid in Bacillus subtilis 168
Tsuge, Kenji, Itaya, Mitsuhiro
Transformation of Bacillus subtilis by a plasmid requires a circular multimeric form. In contrast, linearized plasmids can be circularized only when homologous sequences are present in the host...
Matsumoto, Kouji, Okada, Masahiro, Horikoshi, Yuko, Matsuzaki, Hiroshi, Kishi, Tsutomu, Itaya, Mitsuhiro, ...
The psd gene of Bacillus subtilis Marburg, encoding phosphatidylserine decarboxylase, has been cloned and sequenced. It encodes a polypeptide of 263 amino acid residues (deduced molecular weight of...
Conversion of sub-megasized DNA to desired structures using a novel Bacillus subtilis genome vector
Kaneko, Shinya, Tsuge, Kenji, Takeuchi, Takashi, Itaya, Mitsuhiro
A novel genome vector using the 4215 kb Bacillus subtilis genome provides for precise target cloning and processing of the cloned DNA to the desired structure. Each process highly dependent on...
Tsuge, Kenji, Matsui, Kuniko, Itaya, Mitsuhiro
A universal method to reconstitute sets of genes was developed. Owing to the intrinsic nature of the plasmid establishment mechanism in Bacillus subtilis, the assembly of five antibiotic resistance...
Tomita, Satoshi, Tsuge, Kenji, Kikuchi, Yo, Itaya, Mitsuhiro
A method for positional cloning of the Bacillus subtilis genome was developed. The method requires a set of two small DNA fragments that flank the region to be copied. A 38-kb segment that carries...
Comparative Analysis of Physical Maps of Four Bacillus subtilis (natto) Genomes
Qiu, Dongru, Fujita, Kyoko, Sakuma, Yuko, Tanaka, Teruo, Ohashi, Yoshiaki, Ohshima, Hideyuki, ...
The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome...
Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7
Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro
ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein exhibited RNase H activity in both in vivo and...
Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro
All the archaeal genomes sequenced to date contain a single Type 2 RNase H gene. We found that the genome of a halophilic archaeon, Halobacterium sp. NRC-1, contains an open reading frame with...
Itaya, Mitsuhiro, Tsuge, Kenji, Koizumi, Maki, Fujita, Kyoko
Cloning the whole 3.5-megabase (Mb) genome of the photosynthetic bacterium Synechocystis PCC6803 into the 4.2-Mb genome of the mesophilic bacterium Bacillus subtilis 168 resulted in a 7.7-Mb...
Tsuge, Kenji, Inoue, Satoka, Ano, Takashi, Itaya, Mitsuhiro, Shoda, Makoto
Iturin A and its derivatives are lipopeptide antibiotics produced by Bacillus subtilis and several closely related bacteria. Three iturin group operons (i.e., iturin A, mycosubtilin, and bacillomycin...
Nishizaki, Tomoko, Tsuge, Kenji, Itaya, Mitsuhiro, Doi, Nobuhide, Yanagawa, Hiroshi
We attempted to optimize the production of zeaxanthin in Escherichia coli by reordering five biosynthetic genes in the natural carotenoid cluster of Pantoea ananatis. Newly designed operons for...
Reassessment of the In Vivo Functions of DNA Polymerase I and RNase H in Bacterial Cell Growth▿ †
Fukushima, Sanae, Itaya, Mitsuhiro, Kato, Hiroaki, Ogasawara, Naotake, Yoshikawa, Hirofumi
A major factor in removing RNA primers during the processing of Okazaki fragments is DNA polymerase I (Pol I). Pol I is thought to remove the RNA primers and to fill the resulting gaps...