Mitsuo Yamashita

Interactions of Arsenic with Human Metallothionein-2 (2002)

Toyama, Mitsutoshi, Yamashita, Mitsuo, Hirayama, Noriaki, Murooka, Yoshikatsu

Arsenic is a toxic element that is found in the atmosphere, as well as in aquatic and terrestrial environments- We have demonstrated that As3+ binds to human metallothionein-2 (hMT-2) by UV...

Purification and Characterization of the 3-Ketosteroid-{Delta}1-Dehydrogenase of Arthrobacter simplex Produced in Streptomyces lividans (1995)

Choi, Kwang-Pil, Molnár, István, Yamashita, Mitsuo, Murooka, Yoshikatsu

The 3-ketosteroid-Δ1-dehydrogenase (KS1DH) gene of Arthrobacter simplex IFO12069 cloned in Streptomyces lividans was overexpressed, resulting in production of the enzyme both extracellularly and...

Random Mutagenesis of Pullulanase from Klebsiella aerogenes for Studies of the Structure and Function of the Enzyme (1994)

Yamashita, Mitsuo, Kinoshita, Takuya, Ihara, Michiko, Mikawa, Tomomi, Murooka, Yoshikatu

To study the structure and function of pullulanase from Klebsiella aerogenes, a method involving random mutagenesis of the entire gene for pullulanase was used. Out of 50, 000 clones screened at high...

Characterization of pRGO1, a Plasmid from Propionibacterium acidipropionici, and Its Use for Development of a Host-Vector System in Propionibacteria

Kiatpapan, Pornpimon, Hashimoto, Yoshiteru, Nakamura, Hisako, Piao, Yong-Zhe, Ono, Hisayo, Yamashita, Mitsuo, ...

The complete nucleotide sequence of pRGO1, a cryptic plasmid from Propionibacterium acidipropionici E214, was determined. pRGO1 is 6,868 bp long, and its G+C content is 65.0%. Frame analysis of the...

Molecular Characterization of Lactobacillus plantarum Genes for β-Ketoacyl-Acyl Carrier Protein Synthase III (fabH) and Acetyl Coenzyme A Carboxylase (accBCDA), Which Are Essential for Fatty Acid Biosynthesis

Kiatpapan, Pornpimon, Kobayashi, Hajime, Sakaguchi, Maki, Ono, Hisayo, Yamashita, Mitsuo, Kaneko, Yoshinobu, ...

Genes for subunits of acetyl coenzyme A carboxylase (ACC), which is the enzyme that catalyzes the first step in the synthesis of fatty acids in Lactobacillus plantarum L137, were cloned and...

Effects of Expression of hemA and hemB Genes on Production of Porphyrin in Propionibacterium freudenreichii

Piao, Yongzhe, Kiatpapan, Pornpimon, Yamashita, Mitsuo, Murooka, Yoshikatsu

The genus Propionibacterium has a wide range of probiotic activities that are exploited in dairy and fermentation systems such as cheeses, propionic acid, and tetrapyrrole compounds. In order to...

Characterization of pRGO1, a Plasmid from Propionibacterium acidipropionici, and Its Use for Development of a Host-Vector System in Propionibacteria

Kiatpapan, Pornpimon, Hashimoto, Yoshiteru, Nakamura, Hisako, Piao, Yong-Zhe, Ono, Hisayo, Yamashita, Mitsuo, ...

The complete nucleotide sequence of pRGO1, a cryptic plasmid from Propionibacterium acidipropionici E214, was determined. pRGO1 is 6,868 bp long, and its G+C content is 65.0%. Frame analysis of the...

Molecular Characterization of Lactobacillus plantarum Genes for β-Ketoacyl-Acyl Carrier Protein Synthase III (fabH) and Acetyl Coenzyme A Carboxylase (accBCDA), Which Are Essential for Fatty Acid Biosynthesis

Kiatpapan, Pornpimon, Kobayashi, Hajime, Sakaguchi, Maki, Ono, Hisayo, Yamashita, Mitsuo, Kaneko, Yoshinobu, ...

Genes for subunits of acetyl coenzyme A carboxylase (ACC), which is the enzyme that catalyzes the first step in the synthesis of fatty acids in Lactobacillus plantarum L137, were cloned and...

Effects of Expression of hemA and hemB Genes on Production of Porphyrin in Propionibacterium freudenreichii

Piao, Yongzhe, Kiatpapan, Pornpimon, Yamashita, Mitsuo, Murooka, Yoshikatsu

The genus Propionibacterium has a wide range of probiotic activities that are exploited in dairy and fermentation systems such as cheeses, propionic acid, and tetrapyrrole compounds. In order to...