Site-specific dissection of E.coli chromosome by {lambda} terminase (1992)
Kotani, Hirokazu, Kawamura, Akira, Takahashi, Asako, Nakatsuji, Masako, Hiraoka, Nobutsugu, Nakajima, Kazuo, ...
We have succeeded the targeted cleavage of chromosomes by λ terminase that introduces double-strand cleavages in DNA recognizing the λ cos sequence. When chromosomal DNAs of various Escherichia...
Aoyama, Takashi, Takanami, Mituru, Oka, Atsuhiro
The inducibility of the vir genes (virA, -B, -C, -D, -E, and -G) on pRiA4 was examined at the transcriptional level, and the RNA-starting sites were determined by S1-nuclease mapping and...
Aoyama, Takashi, Takanami, Mituru
Starting from a synthetic E.coli promoter with the consensus sequences at −35 and −10 regions, a sequence CAT frequently occurred in the RNA start points of natural promoters was introduced in...
Aoyama, Takashi, Takanami, Mituru, Ohtsuka, Eiko, Taniyama, Yoshio, Marumoto, Ryuji, Sato, Hiroko, ...
A promoter with the consensus sequences(TTGACA and TATAAT) at −35 and −10 regions was constructed, and the distance between the two consensus sequences was independently altered at two...
Tabata, Satoshi, Oka, Atsuhiro, Sugimoto, Kazunori, Takanami, Mituru, Yasuda, Soichi, Hirota, Yukinon
The replication origin of the E.coli K-12 chromosome has been isolated as autonomously replicating molecules (oriC plasmid), and the DNA region essential for replicating function (oriC) has been...
Asada, Kiyozo, Sugimoto, Kazunori, Oka, Atsuhiro, Takanami, Mituru, Hirota, Yukinori
The replication origin region of the Escherichia coli K-12 chromosome has been cloned, and a region of 245 base-pairs has been shown to contain all the information for autonomous replication (defined...
Kimura, Takao, Sawada, Yukiharu, Shinawawa, Morikazu, Shimizu, Yasuaki, Shiroki, Kazuko, Shimojo, Hiroto, ...
The nudeotide sequence of the entire transforming early region Elb of the highly oncogenic adenovirus type 12 (Adl2) DNA has been determined. The total sequence (3860 base pairs) encompasses the...
Nucleotide sequence of the asnA gene coding for asparagine synthetase of E.coli K-12 (1981)
Nakamura, Masataka, Yamada, Masao, Hirota, Yukinori, Sugimoto, Kazunori, Oka, Atsuhiro, Takanami, Mituru
We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin,...
Electron Microscopy of the Replicative Form DNA of Coliphage fd (1971)
ODA, Takuzo, NAKAMURA, Takashi, WATANABE, Sekiko, TAKANAMI, Mituru
Electron microscopic observations were made on the replicative form DNA of bacteriophage fd. The doubly closed replicative form DNA (RF-1 DNA) was purified from E. coli cells infected with fd, and...