Crystal Structure of the Termination Module of a Nonribosomal Peptide Synthetase (2009)
Alan Tanovic, Stefan A. Samel, Lars-oliver Essen, Mohamed A. Marahiel
Nonribosomal peptide synthetases (NRPS) are modularly organized megaenzymes that act as an assembly line to catalyze the biosynthesis of complex natural products. The crystal structure of the 144 kDa...
MINIREVIEW Learning from Nature’s Drug Factories: Nonribosomal Synthesis of (2009)
Macrocyclic Peptides, Stephan A. Sieber, Mohamed A. Marahiel
With the discovery of penicillin as an efficient antibacterial agent isolated from the fungus Penicillium notatum, microorganisms attracted considerable attention as a new source for pharmaceutical...
HIGHLIGHT www.rsc.org/molecularbiosystems | Molecular BioSystems (2009)
Stefan A. Samel, Mohamed A. Marahiel, Lars-oliver Essen
to tailor non-ribosomal peptide products—new clues about the structures and mechanisms of modifying enzymes
Copper Acquisition Is Mediated by YcnJ and Regulated by YcnK and CsoR in Bacillus subtilis (2009)
Chillappagari, Shashi, Miethke, Marcus, Trip, Hein, Kuipers, Oscar P., Marahiel, Mohamed A.
Copper is an essential cofactor for many enzymes, and at over a threshold level, it is toxic for all organisms. To understand the mechanisms underlying copper homeostasis of the gram-positive...
Module of a Nonribosomal (2008)
Alan Tanovic, Et Al, Alan Tanovic, Stefan A. Samel, Lars-oliver Essen, Mohamed A. Marahiel
The following resources related to this article are available online at
Protein Interactions in Peptide Antibiotic Synthetases (2007)
Er Koglin, Et Al, Er Koglin, Mohammad R. Mofid, Frank Löhr, Birgit Schäfer, ...
The following resources related to this article are available online at
Miethke, Marcus, Westers, Helga, Blom, Evert-Jan, Kuipers, Oscar P., Marahiel, Mohamed A.
Iron deprivation in bacteria causes the derepression of genes controlled by the ferric uptake regulator (Fur). The present microarray analysis of iron-starved Bacillus subtilis cells grown in minimal...
Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH (2003)
Ingelfinger, Dierk, Göthel, Sven F., Marahiel, Mohamed A., Reidt, Ulrich, Ficner, Ralf, Lührmann, Reinhard, ...
Cyclophilins are a family of proteins that share a common, highly conserved sequence motif. Cyclophilins bind transiently to other proteins and facilitate their folding. One member of the family,...
Dissection of the Mechanism for the Stringent Factor RelA (2002)
Wendrich,Thomas M., Blaha,Gregor, Wilson,Daniel N., Marahiel,Mohamed A., Nierhaus,Knud H.
During conditions of nutrient deprivation, ribosomes are blocked by uncharged tRNA at the A site. The stringent factor RelA binds to blocked ribosomes and catalyzes synthesis of (p)ppGpp, a secondary...
Dissection of the Mechanism for the Stringent Factor RelA (2002)
Wendrich, Thomas M., Blaha, Gregor, Wilson, Daniel N., Marahiel, Mohamed A., Nierhaus, Knud H.
During conditions of nutrient deprivation, ribosomes are blocked by uncharged tRNA at the A site. The stringent factor RelA binds to blocked ribosomes and catalyzes synthesis of (p)ppGpp, a secondary...
Weber, Michael H. W., Fricke, Ingo, Doll, Niclas, Marahiel, Mohamed A.
CSDBase (http://www.chemie.uni-marburg.de/~csdbase/) is an interactive Internet-embedded research platform providing detailed information on proteins containing the cold shock domain...
Construction of hybrid peptide synthetases by module and domain fusions
Mootz, Henning D., Schwarzer, Dirk, Marahiel, Mohamed A.
Nonribosomal peptide synthetases are modular enzymes that assemble peptides of diverse structures and important biological activities. Their modular organization provides a great potential for the...
Klein, Wolfgang, Weber, Michael H. W., Marahiel, Mohamed A.
Bacillus subtilis has developed sophisticated mechanisms to withstand fluctuations in temperature. Membrane fatty acids are the major determinants for a sufficiently fluid membrane state to ensure...
Complementation of Cold Shock Proteins by Translation Initiation Factor IF1 In Vivo
Weber, Michael H. W., Beckering, Carsten L., Marahiel, Mohamed A.
The cold shock response in both Escherichia coli and Bacillus subtilis is induced by an abrupt downshift in growth temperature and leads to a dramatic increase in the production of a homologous class...
Weber, Michael H. W., Fricke, Ingo, Doll, Niclas, Marahiel, Mohamed A.
CSDBase (http://www.chemie.uni-marburg.de/~csdbase/) is an interactive Internet-embedded research platform providing detailed information on proteins containing the cold shock domain (CSD). It...
Weber, Michael H. W., Volkov, Arsen V., Fricke, Ingo, Marahiel, Mohamed A., Graumann, Peter L.
Using immunofluorescence microscopy and a fusion of a cold shock protein (CSP), CspB, to green fluorescent protein (GFP), we showed that in growing cells Bacillus subtilis CSPs specifically localize...
Konz, Dirk, Doekel, Sascha, Marahiel, Mohamed A.
Lichenysins are surface-active lipopeptides with antibiotic properties produced nonribosomally by several strains of Bacillus licheniformis. Here, we report the cloning and sequencing of an entire...
May, Jürgen J., Kessler, Nadine, Marahiel, Mohamed A., Stubbs, Milton T.
The synthesis of the catecholic siderophore bacillibactin is accomplished by the nonribosomal peptide synthetase (NRPS) encoded by the dhb operon. DhbE is responsible for the initial step in...
Regeneration of misprimed nonribosomal peptide synthetases by type II thioesterases
Schwarzer, Dirk, Mootz, Henning D., Linne, Uwe, Marahiel, Mohamed A.
Nonribosomal peptide synthetases (NRPSs) assemble structurally complex peptides from simple building blocks such as amino and carboxyl acids. Product release by macrocyclization or hydrolysis is...
Genomewide Transcriptional Analysis of the Cold Shock Response in Bacillus subtilis
Beckering, Carsten L., Steil, Leif, Weber, Michael H. W., Völker, Uwe, Marahiel, Mohamed A.
Previous studies with two-dimensional gel electrophoresis techniques revealed that the cold shock response in Bacillus subtilis is characterized by rapid induction and accumulation of two classes of...
Two protein–protein interaction sites on the spliceosome-associated human cyclophilin CypH
Ingelfinger, Dierk, Göthel, Sven F., Marahiel, Mohamed A., Reidt, Ulrich, Ficner, Ralf, Lührmann, Reinhard, ...
Cyclophilins are a family of proteins that share a common, highly conserved sequence motif. Cyclophilins bind transiently to other proteins and facilitate their folding. One member of the family,...
Hunger, Karen, Beckering, Carsten L., Wiegeshoff, Frank, Graumann, Peter L., Marahiel, Mohamed A.
The nucleic acid binding cold shock proteins (CSPs) and the cold-induced DEAD box RNA helicases have been proposed separately to act as RNA chaperones, but no experimental evidence has been reported...
Chemoenzymatic and Template-Directed Synthesis of Bioactive Macrocyclic Peptides
Grünewald, Jan, Marahiel, Mohamed A.
Non-ribosomally synthesized peptides have compelling biological activities ranging from antimicrobial to immunosuppressive and from cytostatic to antitumor. The broad spectrum of applications in...
Sigma L Is Important for Cold Shock Adaptation of Bacillus subtilis
Wiegeshoff, Frank, Beckering, Carsten L., Debarbouille, Michel, Marahiel, Mohamed A.
Although sigma factor-dependent transcriptional regulation was shown to be essential for adaptation to different environmental stimuli, no such sigma factor has been related to the regulation of the...
Hewald, Sandra, Linne, Uwe, Scherer, Mario, Marahiel, Mohamed A., Kämper, Jörg, Bölker, Michael
Many microorganisms produce surface-active substances that enhance the availability of water-insoluble substrates. Although many of these biosurfactants have interesting potential applications, very...
Construction of hybrid peptide synthetases by module and domain fusions
Mootz, Henning D., Schwarzer, Dirk, Marahiel, Mohamed A.
Nonribosomal peptide synthetases are modular enzymes that assemble peptides of diverse structures and important biological activities. Their modular organization provides a great potential for the...
Klein, Wolfgang, Weber, Michael H. W., Marahiel, Mohamed A.
Bacillus subtilis has developed sophisticated mechanisms to withstand fluctuations in temperature. Membrane fatty acids are the major determinants for a sufficiently fluid membrane state to ensure...
Complementation of Cold Shock Proteins by Translation Initiation Factor IF1 In Vivo
Weber, Michael H. W., Beckering, Carsten L., Marahiel, Mohamed A.
The cold shock response in both Escherichia coli and Bacillus subtilis is induced by an abrupt downshift in growth temperature and leads to a dramatic increase in the production of a homologous class...
Weber, Michael H. W., Fricke, Ingo, Doll, Niclas, Marahiel, Mohamed A.
CSDBase (http://www.chemie.uni-marburg.de/~csdbase/) is an interactive Internet-embedded research platform providing detailed information on proteins containing the cold shock domain (CSD). It...
Weber, Michael H. W., Volkov, Arsen V., Fricke, Ingo, Marahiel, Mohamed A., Graumann, Peter L.
Using immunofluorescence microscopy and a fusion of a cold shock protein (CSP), CspB, to green fluorescent protein (GFP), we showed that in growing cells Bacillus subtilis CSPs specifically localize...
Konz, Dirk, Doekel, Sascha, Marahiel, Mohamed A.
Lichenysins are surface-active lipopeptides with antibiotic properties produced nonribosomally by several strains of Bacillus licheniformis. Here, we report the cloning and sequencing of an entire...
May, Jürgen J., Kessler, Nadine, Marahiel, Mohamed A., Stubbs, Milton T.
The synthesis of the catecholic siderophore bacillibactin is accomplished by the nonribosomal peptide synthetase (NRPS) encoded by the dhb operon. DhbE is responsible for the initial step in...
Regeneration of misprimed nonribosomal peptide synthetases by type II thioesterases
Schwarzer, Dirk, Mootz, Henning D., Linne, Uwe, Marahiel, Mohamed A.
Nonribosomal peptide synthetases (NRPSs) assemble structurally complex peptides from simple building blocks such as amino and carboxyl acids. Product release by macrocyclization or hydrolysis is...
Genomewide Transcriptional Analysis of the Cold Shock Response in Bacillus subtilis
Beckering, Carsten L., Steil, Leif, Weber, Michael H. W., Völker, Uwe, Marahiel, Mohamed A.
Previous studies with two-dimensional gel electrophoresis techniques revealed that the cold shock response in Bacillus subtilis is characterized by rapid induction and accumulation of two classes of...
Two protein–protein interaction sites on the spliceosome-associated human cyclophilin CypH
Ingelfinger, Dierk, Göthel, Sven F., Marahiel, Mohamed A., Reidt, Ulrich, Ficner, Ralf, Lührmann, Reinhard, ...
Cyclophilins are a family of proteins that share a common, highly conserved sequence motif. Cyclophilins bind transiently to other proteins and facilitate their folding. One member of the family,...
Hunger, Karen, Beckering, Carsten L., Wiegeshoff, Frank, Graumann, Peter L., Marahiel, Mohamed A.
The nucleic acid binding cold shock proteins (CSPs) and the cold-induced DEAD box RNA helicases have been proposed separately to act as RNA chaperones, but no experimental evidence has been reported...
Chemoenzymatic and Template-Directed Synthesis of Bioactive Macrocyclic Peptides
Grünewald, Jan, Marahiel, Mohamed A.
Non-ribosomally synthesized peptides have compelling biological activities ranging from antimicrobial to immunosuppressive and from cytostatic to antitumor. The broad spectrum of applications in...
Sigma L Is Important for Cold Shock Adaptation of Bacillus subtilis
Wiegeshoff, Frank, Beckering, Carsten L., Debarbouille, Michel, Marahiel, Mohamed A.
Although sigma factor-dependent transcriptional regulation was shown to be essential for adaptation to different environmental stimuli, no such sigma factor has been related to the regulation of the...
Hewald, Sandra, Linne, Uwe, Scherer, Mario, Marahiel, Mohamed A., Kämper, Jörg, Bölker, Michael
Many microorganisms produce surface-active substances that enhance the availability of water-insoluble substrates. Although many of these biosurfactants have interesting potential applications, very...
Coping with the cold: the cold shock response in the Gram-positive soil bacterium Bacillus subtilis.
Weber, Michael H W, Marahiel, Mohamed A
All organisms examined to date, respond to a sudden change in environmental temperature with a specific cascade of adaptation reactions that, in some cases, have been identified and monitored at the...
Miethke, Marcus, Westers, Helga, Blom, Evert-Jan, Kuipers, Oscar P., Marahiel, Mohamed A.
Iron deprivation in bacteria causes the derepression of genes controlled by the ferric uptake regulator (Fur). The present microarray analysis of iron-starved Bacillus subtilis cells grown in minimal...
Cloning and characterization of the biosynthetic gene cluster for kutznerides
Fujimori, Danica Galonić, Hrvatin, Siniša, Neumann, Christopher S., Strieker, Matthias, Marahiel, Mohamed A., Walsh, Christopher T.
Kutznerides, actinomycete-derived cyclic depsipetides, consist of six nonproteinogenic residues, including a highly oxygenated tricyclic hexahydropyrroloindole, a chlorinated piperazic acid,...
Siderophore-Based Iron Acquisition and Pathogen Control
Miethke, Marcus, Marahiel, Mohamed A.
Summary: High-affinity iron acquisition is mediated by siderophore-dependent pathways in the majority of pathogenic and nonpathogenic bacteria and fungi. Considerable progress has been made in...
Miethke, Marcus, Schmidt, Sarah, Marahiel, Mohamed A.
High-affinity iron acquisition in Bacillus subtilis is mediated via the bacillibactin catechole siderophore pathway. Three of the four essential pathway steps, bacillibactin synthesis,...
Copper Acquisition Is Mediated by YcnJ and Regulated by YcnK and CsoR in Bacillus subtilis▿
Chillappagari, Shashi, Miethke, Marcus, Trip, Hein, Kuipers, Oscar P., Marahiel, Mohamed A.
Copper is an essential cofactor for many enzymes, and at over a threshold level, it is toxic for all organisms. To understand the mechanisms underlying copper homeostasis of the gram-positive...