Morio Ikehara

Role of the Mg2+Ion in the Escherichia coli Ribonuclease HI Reaction (1994)

Uchiyama, Yohtaro, Iwai, Shigenori, Ueno, Yoshio, Ikehara, Morio, Ohtsuka, Eiko

To study the interaction and the role of the metal ion in the reaction catalyzed by Escherichia coli ribonuclease HI (E. coli RNase HI), substrate analogues containing a phosphorothioate linkage or...

Binding of nucleic acids to E.coli RNase HI observed by NMR and CD spectroscopy (1993)

Oda, Yasushi, Iwa, Shigenori, Ohtsuka, Eiko, Ishikawa, Momoyo, Ikehara, Morio, Nakamura, Haruki

To clarify the mechanism by which the RNA portion of a DNA/RNA hybrid is specifically hydrolyzed by ribonuclease H (RNase H), the binding of a DNA/RNA hybrid, a DNA/DNA duplex, or an RNA/RNA duplex...

Crystal Structure of RNase T1(Y45W) Complexed with 3' AMP and Gf1pA (1993)

Gohda, Keigo, Itoh, Takeshi, Hiramatsu, Yasuyuki, Tomita, Ken-ichi, Nishikawa, Satoshi, Uesugi, Sei-ichi, ...

We have previously reported the crystallization of a mutant RNase T1(Y46W) with a synthetic modified trinucleotide ApGflpA [Hakoshima, T. et al. (1990) J. Biochem 108, 695–698]. In the present...

Participation of glutamic acid 23 of T4 endonuclease V in the {beta}-elimination reaction of an abasic site in a synthetic duplex DNA (1992)

Hori, Naoko, Doi, Tomoko, Karaki, Yoko, Kituchi, Masakazu, Ikehara, Morio, Ohtsuka, Eiko

T4 endonuclease V catalyzes the hydrolysis of the glycosyl bond of a thymine dimer in a DNA duplex and the cleavage of the 3′-phosphate by β-elimination. We have previously identified a catalytic...

Structural models of ribonuclease H domains in reverse transcriptases from retroviruses (1991)

Nakamura, Haruki, Katayanagi, Katsuo, Morikawa, Kosuke, Ikehara, Morio

Tertiary models of ribonuclease H (RNase H) domains in reverse transcriptases (RT8) from Moloney murine leukemia virus (MuLV) and human immunodeficiency virus (HIV-1) were built based upon the X-ray...

NMR studies of a DNA containing 8-hydroxydeoxyguanosine (1991)

Oda, Yasushi, Uesugi, Seichi, Ikehara, Morio, Nishimura, Susumu, Kawase, Yasutoshi, Ishikawa, Hiroyuki, ...

The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which...

NMR studies for identification of dl:dG mismatch base-pairing structure in DNA (1991)

Oda, Yasushi, Uesugi, Seiichi, Ikehara, Morio, Kawase, Yasutoshi, Ohtsuka, Eiko

One- and two-dimensional NMR experiments have been undertaken to Investigate deoxylnosine:deoxyguanosine (dl:dG) base pairing in a seff-complementary dodecadeoxyribonucleotide, d(C1...

Hydrophobic effects on protein/nucleic acid interaction: enhancement of substrate binding by mutating tyrosine 45 to tryptophan in ribonuclease Tl (1991)

Hakoshima, Toshio, Tanaka, Masahiro, Itoh, Takeshi, Tomita, Ken-khi, Amisaki, Takashi, Nishikawa, Satoshi, ...

Hydrophobic effects on binding of ribonuclease Tl to guanine bases of several ribonucleotides have been proved by mutating a hydrophobic residue at the recognition site and by measuring the effect on...

Conformation of 2'GMP Bound to a Mutant Ribonuclease T1 (Y45W) Determined by X-Ray Diffraction and NMR Methods (1991)

Itoh, Takeshi, Tomita, Ken-ichi, Hakoshima, Toshio, Hiroaki, Hidekazu, Uesugi, Sei-ichi, Nishikawa, Satoshi, ...

The crystal structure of a mutant ribonuclease T1(Y45W) complexed with a specific inhibitor, 2′GMP, has been determined by X-ray diffraction and refined at 1.9 A� resolution to a conventional...

A thermoresistant mutant of ribonuclease T1 having three disulfide bonds (1990)

Nishikawa, Satoshi, Adiwinata, Jeanne, Morioka, Hiroshi, Fujimura, Takao, Tanaka, Toshiki, Uesugi, Seiichi, ...

Molecular-dynamic calculations predict that, if Tyr24 and Asn84 are each replaced by a Cys residue, it should be possible to form a third disulfide bond in ribonuclease T1 (RNase T1) between these...

Crystallographic Characterization of Wild-Type and Mutant Ribonuclease T1 Complexes with Several Ribonucleotides (1990)

Hakoshima, Toshio, Oka, Ken-ichi, Toda, Shyoji, Tanaka, Masahiro, Goda, Keigo, Higo, Takashi, ...

Ribonuclease T1 and the mutant enzymes were cocrystallized with several ribonucleotides, including non-hydrolyzable substrate analogs of di- and triribonucleotides, which have a novel guanylate in...

Structure and activity of artificial mutant variants of human growth hormone (1989)

Nishikawa, Satoshi, Nishida, Yoshitaka, Uemura, Haruki, Yamada, Yasuki, Tanaka, Toshiki, Uesugi, Seiichi, ...

Four artificial mutant variants of human growth hormone (hGH) with the following characteristics were prepared in Escherichia coli by in vitro mutagenesis: (i) replacement of Trp86 with Tyr (W86Y...

Conformational properties of the guanine-binding site of ribonuclease T1 inferred from the X-ray structure and protein engineering (1988)

Hakoshima, Toshio, Toda, Shoji, Sugio, Shigetoshi, Tomita, Ken-ichi, Nishikawa, Satoshi, Morioka, Hiroshi, ...

Recognition by ribonuclease T1 of guanine bases via multidentate hydrogen bonding and stacking interactions appears to be mediated mainly by a short peptide segment formed by one stretch of a...

Conversion of the guanine nucleotide binding sites of ras protein resulting in the reduction of base specificity (1988)

Miura, Kazunobu, Kamiya, Hiroyuki, Kubota, Sachie, Ikehara, Morio, Nishimura, Susumu, Ohtsuka, Eiko

A gene coding for the novel ras protein, p21x, in which the domains of guanine binding and phosphate binding were exchanged, was constructed and expressed in Escherichia coli. The gene product, p21x,...

Solid phase synthesis of oligoribonucleotides using the o-nitrobenzyl group for 2'-hydroxyl protection and H-phosphonate chemistry (1987)

Tanaka, Toshiki, Tamatsukuri, Shigeru, Ikehara, Morio

Oligoribonucleotides with chain length of 7, 11, 15, 17, 24 and 34 were synthesized on long chain alkylamine controlled pore glass beads (LCA-CPG) using o-nitrobenzyl protection of 2'-hydroxyis via a...

Synthesis of oligodeoxyribonucleotide with aliphatic amino or phosphate group at the 5' end by the phosphotriester method on a polystyrene support (1987)

Tanaka, Toshiki, Sakata, Takeshi, Fujimoto, Kazuko, Ikehara, Morio

Lipophilic protecting groups mTrNHfCH2)n X (mTr: monomethoxy-trityl, X=NH, O, S, n=2, 3, 4, 6) were attachecd to the 51-phosphoryl group of 3′-O-protected thymidine. When the diamine derivatives...

Efficient cleavage by {alpha}-thrombin of a recombinant fused protein which contains insulin-like growth factor I (1987)

Nishikawa, Satoshi, Yanase, Kohichi, Tokunaga-Doi, Tomoko, Kodama, Kazumi, Gomi, Hideyuki, Uesugi, Seiichi, ...

The gene for insulin-like growth factor I (IGF-I) was constructed from chemically synthesized deoxyoligonucleotides and expressed in Escherichia coli, under the control of a trp promoter, as a set of...

Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2'-hydroxyl via a phosphite triester approach (1986)

Tanaka, Toshiki, Tamatsukuri, Shigeru, Ikehara, Morio

The hepta and undecaribonucleotide were synthesized on a controlled pore glass beads using o-nitrobenzyl protection of 2′-hydroxyls via a phosphite approach. By using 5-p-nitrophenyltetrazole for...

Replacement and Insertion of nucleotides at the anticodon loop of E. coli tRNAfMet by ligation of chemically synthesized ribooligonucleotides (1985)

Doi, Takefumi, Yamane, Akio, Matsugi, Jitsuhiro, Ohtsuka, Eiko, Ikehara, Morio

Insertion of the four major nucleotides at the 5′-side of the anticodon triplet of E. coli tRNAMet was performed by joining of the half molecules obtained b limited digestion with RNase A and the...

Dynamic Behavior of the Imino Protons of the {lambda} OR3 17mer in H2O Solution Studied by High-Resolution NMR (1985)

LEE, Sang Jong, AKUTSU, Hideo, KYOGOKU, Yoshimasa, KITANO, Katsuhiko, TOZUKA, Zenzaburo, OHTA, Atsunori, ...

The imino proton resonances of λ OR3 17mer in water were observed at 500 Mhz with the time-shared Redfield pulse train. All of the 17 imino proton resonances could be assigned specifically to...

Molecular Cloning and Nucleotide Sequence of cDNA Coding for Rat Brain Cholecystokinin Precursor (1984)

KUWANO, Ryozo, ARAKI, Kazuaki, USUI, Hiroshi, FUKUI, Toshikazu, OHTSUKA, Eiko, IKEHARA, Morio, ...

A mixture of 14-mer oligodeoxynucleotides was used for the screening of a cDNA clone coding for a cholecystokinin (CCK) precursor from a cDNA library for rat brain microsomal poly(A)RNA. The longest...

Synthesis and conformational studies of ribooligonucleotides which contain an alternating C-G sequence and show unusual circular dichroism spectra (1984)

Uesugi, Seiichi, Ohkubo, Mitsuru, Ohtsuka, Eiko, Ikehara, Morio, Kobayashi, Yuji, Kyogoku, Yoshimasa

The poly [r(C-G)]duplex shows an unusually large negative CD band in the long wavelength region. in order to elucidate this phenomenon, r(C-G-C-G) and r(C-G-C-G-C-G) were synthesized by a...

Identification of oligonucleotide fragments produced in a strand scisslon reaction of the d(C-G-C-G-C-G) duplex by bleomycin (1984)

Uesugi, Seiichi, Shida, Toshio, Ikehara, Morio, Kobayashi, Yuji, Kyogoku, Yoshimasa

To elucidate the mechanism of DNA strand scission by bleomycin, a d(C-G C-G-C-G) duplex was treated with the bleomycin-iron ion complex in the presence of H2 O2 and degradation products (1, 2,...

Molecular cloning and the complete nucleotide sequence of cDNA to mRNA for S-100 protein of rat brain (1984)

Kuwano, Ryozo, Usui, Hiroshi, Maeda, Toshinaga, Fukui, Toshikazu, Yamanari, Nobuko, Ohtsuka, Eiko, ...

The complete nucleotide sequence of mRNA for β-subunit of rat brain S-100 protein was determined from recombinant cDNA clones. The sequence was composed of 1488 bp which Included the 276 bp of the...

Raman diagnosis of nucleic acid structure: sugar-puckering and glycosidic conformation in the guanosine moiety (1983)

Nishimura, Yoshifumi, Tsuboi, Masamichi, Nakano, Toru, Higuchi, Shigesada, Sato, Tomohiro, Shida, Toshio, ...

Observations of Raman spectra of various nucleic acids indicate that the guanine ring breathing frequency is sensitive to the internal rotation angle around the glycosidic bond and to the...

Modification of the anticodon triplet of E.coli tRNAfMet by replacement with trimers complementary to non-sense codons UAG and UAA (1983)

Ohtsuka, Eiko, Doi, Takefumi, Fukumoto, Ryoichi, Matsugi, Jitsuhiro, Ikehara, Morio

E. coli tRNAfMet was hydrolyzed with RNase A using a limited amount of the enzyme to give two half molecules lacking the anticodon trimer and 3′-terminal dimer. Chemically synthesized trimers CUAp...

E. coli initiator tRNA analogs with different nucleotides in the discriminator base position (1982)

Uemura, Haruki, Imai, Mayumi, Ohtsuka, Eiko, Ikehara, Morio, Söll, Dieter

The effect of base changes at the fourth position from the 3′-terminus of Esoheriohia coli initiator tRNA Met has been studied to test the ′discriminator hypothesis′ which proposed that the...

Reverse Transcriptase Associated with Avian Sarcoma-Leukosis Viruses. III. Influence of Primer Chain Length on the Initiation of DNA Synthesis (1982)

FUKUI, Toshikazu, ISHIHAMA, Akira, OHTSUKA, Eiko, IKEHARA, Morio, FUKUDA, Ryuji

The influence of oligodeoxynucleotide chain length on the priming activity of the reverse transcription was investigated in poly(A)-dependent poly(dT), poly(C)-dependent poly(dG) and influenza virus...

The crystal and molecular structure of 2':-deoxy-2':-fluoroinosine monohydrate (1981)

Hakoshima, Toshio, Omori, Hisako, Tomita, Ken-ichi, Miki, Hiroko, Ikehara, Morio

The structure of the hydrate of 2′-deoxy-2′-fluoroinosine has been determined by single-crystal x-ray diffraction. The nucleoslde crystallises inspace group P212121, with unit cell dimensions, a...

Preparation of a New Fluorescent Analog of ATP, 2'-(5-Dimethyl-aminonaphthalene-1-sulfonyl)amino-2'-deoxy ATP, and Its Interactions with Myosin and Actomyosin (1981)

WATANABE, Takahide, INOUE, Akio, TONOMURA, Yuji, UESUGI, Seiichi, IKEHARA, Morio

A fluorescent ATP analog, 2′-(5-dimethylaminonaphthalene-1-sulfonyl)amino-2′-deoxy ATP (DNS-ATP), was synthesized. In water, the wavelengths of maximum excitation were 260 and 340 nm, and that of...

Characterization of nascent DNA fragments produced by excision of uracil residues in DNA (1981)

Machida, Yasunori, Okazaki, Tuneko, Miyake, Tetsuo, Ohtsuka, Eiko, Ikehara, Morio

Nascent short DNA chains could result from repair of incorporated uracil residues or be intermediates in discontinuous replication. We have characterized short DNA chains having...

RNA Polymerase of Influenza Virus. II. Influence of Oligonucleotide Chain Length on the Priming Activity of RNA Synthesis by Virion-Associated RNA Polymerase (1981)

KAWAKAMI, Kiyoshi, ISHIHAMA, Akira, OHTSUKA, Eiko, TANAKA, Toshiki, TAKASHIMA, Hideaki, IKEHARA, Morio

The virion-associated RNA-dependent RNA polymerase of influenza virus PR8 was activated by treatment with specific non-ionic detergents, and was remarkably stimulated by exogenously added...

Comparison of substrate base sequences for RNA ligase reactions in the synthesis of a tetradecanucleotide corresponding to bases 21-34 of E. coli tRNAfMet 1 (1980)

Ohtsuka, Eiko, Doi, Takefumi, Uemura, Haruki, Taniyama, Yoshio, Ikehara, Morio

A tetradecanucleotide U-A-G-C (U-C-G)2G-G-C-Up corresponding to bases 21–34 of a nascent sequence of formylmethionyl tRNA of E. coli has been synthesized by the joining of two combinations of...

2'-Deoxy-2'-Azidoadenosine Triphosphate and 2'-Deoxy-2'-Fluoroadenosine Triphosphate as Substrates and Inhibitors for Escherichia coli DNA-dependent RNA Polymerase (1980)

ISHIHAMA, Akira, ENAMI, Masayoshi, NISHIJIMA, Yoshikazu, FUKUI, Toshikazu, OHTSUKA, Eiko, IKEHARA, Morio

The effects of 2′-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the...

Polynucleotides. LVII.1 Synthesis and properties of poly (2'-chloro-2'-deoxyinosinic acid) (1979)

Kakiuchi, Nobuko, Fukui, Toshikazu, Ikehara, Morio

Poly(2′-chloro-2′-deoxyinosinic acid) [poly (Icl)] was synthesized from Icl 5′ -DP by polymerization with polynudleotide phosphorylase. UV absorption properties of poly (Icl) are very similar...

Synthesis of E.coli tRNAfMet fragments and their joining with RNA ligase (1978)

Ohtsuka, Eiko, Tanaka, Toshiki, Miyake, Tetsuo, Nakagawa, Eiko, Antkowiak, Janina, Wakabayashi, Toshiaki, ...

Oligonucleotides corresponding to the E. coli tRNAfMet have been synthesized. A diester approach has been used for the synthesis of 6, 7, 8 and 9. A block condensation of nucleotides with...

Polynucleotides. LVI1. Synthesis and properties of poly(2'-deoxy-2'-fluoroinosinic acid) (1978)

Ikehara, Morio, Kakiuchi, Nobuko, Fukui, Toshikazu

Poly(2′ -deoxy-2′ -fluoroinosinic acid) [poly (If)] was synthesized by polymerization of 2′ -deoxy-2′ -fluoroinosine 5′ -diphosphate catalyzed by Escherichia coli polynucleotide...

Polynucleotides. XLV1) Synthesis and properties of poly(2'-azido-2'-deoxyinosinic acid) (1977)

Fukui, Toshikazu, Kakiuchi, Nubuko, Ikehara, Morio

Poly (2′-azido-2′-deoxyinosinic acid), [poly (Iz)] , was synthesized from 2′-azido-2′-deoxyinosine diphosphate by the action of polynucleotide phosphorylase. Poly (Iz) has UV absorption...

Biologic activities of poly (2-azaadenylic acid) and poly (2-azainosinic acid) (1977)

Clercq, Erik De, Huang, Guang-Fu, Torrence, Paul F., Fukui, Toshikazu, Kakiuchi, Nobuko, Ikehara, Morio

Poly(2-azaadenylic acid) ((aza2A)n) and poly(2-azainosinic acid ((aza2l)n), two newly synthesized analogues of (A)n and (I)n, in which CH-2 of the purine ring is replaced by a nitrogen atom, have...

Polynucleotides. XLVI. 1 Synthesis and properties of poly (2'-amino-2'-deoxyadenylic acid) (1977)

Ikehara, Morio, Fukui, Toshikazu, Kakiuchi, Nobuko

Poly (2′-amino-2′-deoxyadenylic acid) [poly (Aa)] was prepared from chemically synthesized 2′-amino-2′-deoxy-ADP by the catalysis of polynucleotide phosphorylase. Poly (Aa) showed a similar...

Polynucleotides. L. Synthesis and properties of poly (2' - chloro - 2' - deoxyadenylic acid) and poly (2' - bromo - 2' - deoxyadenylic acid) (1977)

Ikehara, Morio, Fukui, Toshikazu, Kakiuchi, Nobuko

Poly (2′-chloro-2′-deoxyadenylic acid) and poly (2′-bromo- 2′-deoxyadenylic acid) were synthesized from the corresponding diphosphates with the aid of polynucleotide phosphorylase from E....

Polynucleotides. XLII. Limited addition of 2'-O-o-nitrobenzyl nucleotides to the 3'-end of ribooligonucleotide with polynucleotide phosphorylase (1976)

Ikehara, Morio, Tanaka, Shoji, Fukui, Toshikazu, Ohtsuka, Eiko

2′-O-o-Nitrobenzyluridine, -cytidine and -adenosine were phosphorylated with phosphoryl chloride to the corresponding 5′-phosphates and led to 5′-diphosphates by the method of Moffatt and...

Structure and Function of the Two Heads of the Myosin Molecule: IV. Physiological Functions of Various Reaction Intermediates in Myosin Adenosinetriphosphatase, Studied by the Interaction between Actomyosin and 8-Bromoadenosine Triphosphate (1976)

TAKENAKA, Hitoshi, IKEHARA, Morio, TONOMURA, Yuji

The kinetic properties of the hydrolyses of 8-Br ATP and 8-SCH3 ATP by myosin [EC 3.6.1.3] and actomyosin were compared with those of ATP, and the following results were obtained. The Ca-NTPase...

Role of purine N-3 in the biologic activities of poly(A) and poly(I) (1976)

De Clercq, Erik, Torrence, Paul F., Fukui, Toshikazu, Ikehara, Morio

Poly(c3A) (poly 3-deazaadenylic acid) and poly(c3I) (poly 3-deazainosinic acid) differ in biological reactivity from their parent compounds poly(A) and poly(I) and from their 7-deaza counterparts...

Joining of ribooligonucleotides with T4 RNA ligase and identification of the oligonucleotide-adenylate intermediate. (1976)

Ohtsuka, Eiko, Nishikawa, Satoshi, Sugiura, Masahiro, Ikehara, Morio

T4 RNA ligase was found to join A-A-A-A-A-A and 32pU-U-U-U in the presence of ATP as cofactor. In this reaction the pyrophosphate of *pU-U-U-U and pA was isolated by chromatography on a RPC-5 column,...

Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid (1976)

Ikehara, Morio, Fukui, Toshikazu, Kakiuchi, Nobuko

Poly 2′-azido-2′-deoxyadenylic acid (Poly Az) was synthesized from 2′-azido-2′-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to...

Inhibition of oncornavirus functions by poly (2-methylthioinosinic acid) (1975)

De Clercq, Erik, Billiau, Alfons, Hattori, Masao, Ikehara, Morio

Poly (2-methylthioinosinic acid) [poly(ms2I)] was found to markedly inhibit the RNA directed DNA polymerase (reverse transcriptase) activity of murine (Moloney, Rauscher) leukemia virus and murine...

Antiviral activity of polynucleotides: copolymers of inosinic acid and N2-dimethylguanylic or 2-methylthioinosinic acid (1975)

Clercq, Erik De, Hattori, Masao, Ikehara, Morio

Complexes of poly(C) with2 copolymers of inosinic acid containing various amounts of mismatched bases (m2G or ms2 I) have been examined for direct resistance to virus infection, interferon induction...

Polynucleotides. XXXII.1 Further studies on the synthesis of oligonucleotides containing 8, 2' -S-cycloadenosine (1975)

Ikehara, Morio, Tezuka, Tom

A dinucleoside monophosphate, 8, 2′-anhydro-8-mercapto-9-β-D-arabinofuranosyladenine phosphoryl-(3′-5′)-inosine (As pI) was synthesized by the condensation of protected 8-mercaptoadenosine...

Seven-membered cyclic phosphate: synthesis and properties of S-cycloadenosine 2' 5'-cyclic phosphate and cordycepin 2', 5'-cyclic phosphate. Studies of nucleosides and nucleotides. LXIV'l). Purine cyclonucleosides 25. (1974)

Ikehara, Morio, Yano, Junichi

8,3′-Anhydro-8-mercapto-9-β-D-xylofuranosyladenine (8,3′-S-cycloadenosine) was phosphorylated with cyanoethyl phosphate and DCC to 5′-phosphate. After 6-amino group was benzoylated, the...

Polynucleotides. XXII(1). Synthesis and properties of poly 7-deazainosinic acid (1974)

Ikehara, Morio, Fukui, Toshikazu, Koide, Toshiko, Aba, Junko In

Poly 7-deazainosinic acid has been prepared by the deamination and phosphorylation of tubercidin and the nucleoside diphosphate was polymerised using polynucleotide phosphorylase. The polymer has...

Polynucleotides. XXIII(1). A synthesis of ribodinucleoside monophosphates using nucleoside 5'-phosphates (1974)

Ohtsuka, Eiko, Nakamura, Sojiro, Yoneda, Mariko, Ikehara, Morio

Guanylyl-(3′-5′)-cytidine and guanylyl-(2′-5′)-cytidine were synthesized by condensing 5′-O-monamethoxytritylguanosine and N,2′,3′-O-triacetylcytidine 5′-phosphate using...

Polynucleotides. XXVI. 1) Complex formation of polynucleotides derived from formycin and laurusin with cyclonucleoside oligonucleotides. (1974)

Ikehara, Morio, Tezuka, Toru

Poly(formycin phosphate) and poly (laurusin phosphate) were synthesized by polymerizing formycin and laurusin 5′-diphosphate by means of E. coli polynucleotide phosphorylase. The complex formation...

Polynucleotides. XXIV1. Synthesis and properties of a dinucleoside monophosphate derived from uridine 6,2-cyclonucleoside2 (1974)

Ikehara, Morio, Tezuka, Toru

A dinucleoside ncnophosphate, 6,2′-anhydro-6-oxy-1-β-D–arabinofuranosyluracil-phosphoryl-(3′-5′)-6,2′-anhydro-6-oxy-1-β-D-arabinofuranosyluracil (I) was synthesized by the condensation...

Polynucleotides: XXI. Synthesis and Properties of Poly 1-Deazaadenylic Acid and Poly 3-Deazaadenylic Acid (1974)

IKEHARA, Morio, FUKUI, Toshikazu, UESUGI, Seiichi

Poly 1-deazaadenylic acid (poly 1-deazaA) and poly 3-deazaadenylic acid (poly 3-deazaA) were synthesized from 1-deaza and 3-deaza ADP by the use of E. coli polynucleotide phosphorylase [EC 2.7.7.8]....

Energy Transfer from Tryptophan Residues to a Fluorescent ATP Analog, 1, N6-Ethenoadenosine Tri phosphate, Bound to H-Meromyosin (1973)

ONISHI, Hirofumi, OHTSUKA, Eiko, IKEHARA, Morio, TONOMURA, Yuji

1. The hydrolysis of 1, N6-ethenoadenosine triphosphate (ϵATP) by myosin [EC 3.6.1.3] and the change in fluorescence of ϵATP induced by H-meromyosin were studied. (a) The amount of...

Polynucleotides XVII: Effect of the Diphosphates of Aristeromycin and Cycloadenosine on the Polymerization of Adenosine Diphosphate by Polynucleotide Phosphorylase (1973)

IKEHARA, Morio, FUKUI, Toshikazu

Aristeromycin 5'-diphosphate (ArDP) and 8, 2'-anhydro-8-mercapto-9-β-D-arabinofuranosyladenine 5'-diphosphate (cycloadenosine DP or ASDP) were synthesized and tested for their properties in the...

Interaction between Synthetic ATP Analogues and Actomyosin Systems. IV (1967)

TONOMURA, YUJI, IMAMURA, KIICHI, IKEHARA, MORIO, UNO, HITOSHI, HARADA, FUMIO

The following compounds were synthesized as analogues of ATP: Tubercidin 5′triphosphate (II), 2′ 3′-O-isopropylidene 6-chloro-(9-β-D-ribofuranosyl) purine 5′triphosphate (III) and 2′...

Polynucleotides. LII1 Synthesis and properties of poly(2′-deoxy-2′-fluoroadenylic acid)

Ikehara, Morio, Fukui, Toshikazu, Kakiuchi, Nobuko

2′-Deoxy-2′-fluoroadenosine was chemically transformed to its 5′-diphosphate and polymerized with polynucleotide phosphorylase to give poly(2′-deoxy-2′-fluoroadenylic acid) [poly(Af)]....

Polynucleotides. XXII(1). Synthesis and properties of poly 7-deazainosinic acid

Ikehara, Morio, Fukui, Toshikazu, Koide, Toshiko, Inaba, Junko

Poly 7-deazainosinic acid has been prepared by the deamination and phosphorylation of tubercidin and the nucleoside diphosphate was polymerised using polynucleotide phosphorylase. The polymer has...

Polynucleotides. XXVI1) Complex formation of polynucleotides derived from formycin and laurusin with cyclonucleoside oligonucleotides

Ikehara, Morio, Tezuka, Toru

Poly(formycin phosphate) and poly(laurusin phosphate) were synthesized by polymerizing formycin and laurusin 5′-diphosphate by means of E. coli polynucleotide phosphorylase. The complex formation...

Seven-membered cyclic phosphate: synthesis and properties of S-cycloadenosine 2′,5′-cyclic phosphate and cordycepin 2′,5′-cyclic phosphate. Studies of nucleosides and nucleotides. LXIV1). Purine cyclonucleosides 25

Ikehara, Morio, Yano, Junichi

8,3′-Anhydro-8-mercapto-9-β-D-xylofuranosyladenine (8,3′-s-cycloadenosine) was phosphorylated with cyanoethyl phosphate and DCC to 5′-phosphate. After 6-amino group was benzoylated, the...

Polynucleotides. XXIV1. Synthesis and properties of a dinucleoside monophosphate derived from uridine 6,2-cyclonucleoside2

Ikehara, Morio, Tezuka, Toru

A dinucleoside monophosphate, 6,2′-anhydro-6-oxy-1-β-D-arabinofuranosyluracil-phosphoryl- (3′-5′)-6,2′-anhydro-6-oxy-1-β-D-arabinofuranosyluracil (I) was synthesized by the condensation...

Molecular structure of a double helix that has non-Watson—Crick type base pairing formed by 2-substituted poly(A) and poly(U)

Hakoshima, Toshio, Fukui, Toshikazu, Ikehara, Morio, Tomita, Ken-ichi

X-ray fiber diffraction studies of duplexes formed by 2-substituted poly(A) and poly(U) provide evidence for the existence of a double helical structure of poly(A)·poly(U) held together by...

Polynucleotides. LII1 Synthesis and properties of poly(2′-deoxy-2′-fluoroadenylic acid)

Ikehara, Morio, Fukui, Toshikazu, Kakiuchi, Nobuko

2′-Deoxy-2′-fluoroadenosine was chemically transformed to its 5′-diphosphate and polymerized with polynucleotide phosphorylase to give poly(2′-deoxy-2′-fluoroadenylic acid) [poly(Af)]....

Polynucleotides. XXII(1). Synthesis and properties of poly 7-deazainosinic acid

Ikehara, Morio, Fukui, Toshikazu, Koide, Toshiko, Inaba, Junko

Poly 7-deazainosinic acid has been prepared by the deamination and phosphorylation of tubercidin and the nucleoside diphosphate was polymerised using polynucleotide phosphorylase. The polymer has...

Polynucleotides. XXVI1) Complex formation of polynucleotides derived from formycin and laurusin with cyclonucleoside oligonucleotides

Ikehara, Morio, Tezuka, Toru

Poly(formycin phosphate) and poly(laurusin phosphate) were synthesized by polymerizing formycin and laurusin 5′-diphosphate by means of E. coli polynucleotide phosphorylase. The complex formation...

Seven-membered cyclic phosphate: synthesis and properties of S-cycloadenosine 2′,5′-cyclic phosphate and cordycepin 2′,5′-cyclic phosphate. Studies of nucleosides and nucleotides. LXIV1). Purine cyclonucleosides 25

Ikehara, Morio, Yano, Junichi

8,3′-Anhydro-8-mercapto-9-β-D-xylofuranosyladenine (8,3′-s-cycloadenosine) was phosphorylated with cyanoethyl phosphate and DCC to 5′-phosphate. After 6-amino group was benzoylated, the...

Polynucleotides. XXIV1. Synthesis and properties of a dinucleoside monophosphate derived from uridine 6,2-cyclonucleoside2

Ikehara, Morio, Tezuka, Toru

A dinucleoside monophosphate, 6,2′-anhydro-6-oxy-1-β-D-arabinofuranosyluracil-phosphoryl- (3′-5′)-6,2′-anhydro-6-oxy-1-β-D-arabinofuranosyluracil (I) was synthesized by the condensation...

Molecular structure of a double helix that has non-Watson—Crick type base pairing formed by 2-substituted poly(A) and poly(U)

Hakoshima, Toshio, Fukui, Toshikazu, Ikehara, Morio, Tomita, Ken-ichi

X-ray fiber diffraction studies of duplexes formed by 2-substituted poly(A) and poly(U) provide evidence for the existence of a double helical structure of poly(A)·poly(U) held together by...