NF90 selectively represses the translation of target mRNAs bearing an AU-rich signature motif (2010)
Kuwano, Yuki, Pullmann, Rudolf, Marasa, Bernard S., Abdelmohsen, Kotb, Lee, Eun Kyung, Yang, Xiaoling, ...
The RNA-binding protein nuclear factor 90 (NF90) has been implicated in the stabilization, transport and translational control of several target mRNAs. However, a systematic analysis of NF90 target...
Identification of a signature motif in target mRNAs of RNA-binding protein AUF1 (2009)
Mazan-Mamczarz, Krystyna, Kuwano, Yuki, Zhan, Ming, White, Elizabeth J., Martindale, Jennifer L., Lal, Ashish, ...
The ubiquitous RNA-binding protein AUF1 promotes the degradation of some target mRNAs, but increases the stability and translation of other targets. Here, we isolated AUF1-associated mRNAs by...
HuR recruits let-7/RISC to repress c-Myc expression (2009)
Kim, Hyeon Ho, Kuwano, Yuki, Srikantan, Subramanya, Lee, Eun Kyung, Martindale, Jennifer L., Gorospe, Myriam
RNA-binding proteins (RBPs) and microRNAs (miRNAs) are potent post-transcriptional regulators of gene expression. Here, we show that the RBP HuR reduced c-Myc expression by associating with the c-Myc...
The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA (2009)
López De Silanes, Isabel, Gorospe, Myriam, Taniguchi, Hiroaki, Abdelmohsen, Kotb, Srikantan, Subramanya, Alaminos, Miguel, ...
The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression...
Zhang, Xian, Zou, Tongtong, Rao, Jaladanki N., Liu, Lan, Xiao, Lan, Wang, Peng-Yuan, ...
The X chromosome-linked inhibitor of apoptosis protein (XIAP) is the most potent intrinsic caspase inhibitor and plays an important role in the maintenance of intestinal epithelial integrity. The RNA...
Nuclear HuR accumulation through phosphorylation by Cdk1 (2008)
Kim, Hyeon Ho, Abdelmohsen, Kotb, Lal, Ashish, Pullmann, Rudolf, Yang, Xiaoling, Galban, Stefanie, ...
A predominantly nuclear RNA-binding protein, HuR translocates to the cytoplasm in response to stress and proliferative signals, where it stabilizes or modulates the translation of target mRNAs. Here,...
AGEMAP: A Gene Expression Database for Aging in Mice (2007)
Jacob M. Zahn, Suresh Poosala, Art B. Owen, Donald K. Ingram, Ana Lustig, Arnell Carter, ...
We present the AGEMAP (Atlas of Gene Expression in Mouse Aging Project) gene expression database, which is a resource that catalogs changes in gene expression as a function of age in mice. The AGEMAP...
AGEMAP: a gene expression database for aging in mice (2007)
Jacob M. Zahn, Suresh Poosala, Art B. Owen, Donald Ingram, Ana Lustig, Arnell Carter, ...
We present the AGEMAP gene expression database, which is a resource that catalogs changes in gene expression as a function of age in mice. The AGEMAP database includes expression changes for 8,932...
En masse nascent transcription analysis to elucidate regulatory transcription factors (2006)
Fan, Jinshui, Zhan, Ming, Shen, Jikui, Martindale, Jennifer L., Yang, Xiaoling, Kawai, Tomoko, ...
Despite exhaustively informing about steady-state mRNA abundance, DNA microarrays have been used with limited success to identify regulatory transcription factors (TFs). The main limitation of this...
Cheadle, Chris, Fan, Jinshui, Cho-Chung, Yoon S, Werner, Thomas, Ray, Jill, Do, Lana, ...
Abstract Background Microarray technology has become highly valuable for identifying complex global changes in gene expression patterns. The effective correlation of observed changes in gene...
Antiapoptotic function of RNA-binding protein HuR effected through prothymosin α (2005)
Ashish Lal, Tomoko Kawai, Xiaoling Yang, Krystyna Mazan-Mamczarz, Myriam Gorospe
Increased stability of the p16 mRNA with replicative senescence (2005)
Wengong Wang, Jennifer L Martindale, Xiaoling Yang, Francis J Chrest, Myriam Gorospe
Bmc Genomics, Chris Cheadle, Jinshui Fan, Yoon S Cho-chung, Thomas Werner, Jill Ray, ...
Research article Control of gene expression during T cell activation: alternate regulation of mRNA transcription and mRNA stability
Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs (2004)
Ashish Lal, Krystyna Mazan-Mamczarz, Tomoko Kawai, Xiaoling Yang, Jennifer L Martindale, Myriam Gorospe
Gorospe, Myriam., State University Of New York At Albany. Dept. Of Biological Sciences.
Thesis (Ph.D.) -- The University at Albany, State University of New York, Dept. of Biological Sciences, 1993.
HuR Regulates p21 mRNA Stabilization by UV Light
Wang, Wengong, Furneaux, Henry, Cheng, Huiming, Caldwell, M. Craig, Hutter, Dorothy, Liu, Yusen, ...
Expression of the cyclin-dependent kinase inhibitor p21 is highly induced by many stresses, including exposure to short-wavelength UV light (UVC), which increases p21 mRNA stability. Investigation...
Potapova, Olga, Gorospe, Myriam, Dougherty, Ryan H., Dean, Nicholas M., Gaarde, William A., Holbrook, Nikki J.
c-Jun N-terminal kinase (JNK) plays a critical role in coordinating the cellular response to stress and has been implicated in regulating cell growth and transformation. To investigate the...
Lin, Shankung, Wang, Wengong, Wilson, Gerald M., Yang, Xiaoling, Brewer, Gary, Holbrook, Nikki J., ...
Prostaglandin A2 (PGA2), an experimental chemotherapeutic agent, causes growth arrest associated with decreased cyclin D1 expression in several cancer cell lines. Here, using human non-small-cell...
Loss of HuR Is Linked to Reduced Expression of Proliferative Genes during Replicative Senescence
Wang, Wengong, Yang, Xiaoling, Cristofalo, Vincent J., Holbrook, Nikki J., Gorospe, Myriam
Cellular aging is accompanied by alterations in gene expression patterns. Here, using two models of replicative senescence, we describe the influence of the RNA-binding protein HuR in regulating the...
Li, Ji, Gorospe, Myriam, Hutter, Dorothy, Barnes, Janice, Keyse, Stephen M., Liu, Yusen
Mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) has been shown to play a critical role in mediating the feedback control of MAP kinase cascades in a variety of cellular processes,...
Gorospe, Myriam, Wang, Xiantao, Holbrook, Nikki J.
Exposure of mammalian cells to adverse stimuli triggers the expression of numerous stress response genes, many of which are presumed to enhance cell survival. In this study, we examined the...
Gorospe, Myriam, Egan, Josephine M., Zbar, Berton, Lerman, Michael, Geil, Laura, Kuzmin, Igor, ...
The absence of functional von Hippel-Lindau (VHL) tumor suppressor gene leads to the development of neoplasias characteristic of VHL disease, including renal cell carcinoma (RCC). Here, we compared...
Global analysis of stress-regulated mRNA turnover by using cDNA arrays
Fan, Jinshui, Yang, Xiaoling, Wang, Wengong, Wood, William H., Becker, Kevin G., Gorospe, Myriam
cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the...
AMP-Activated Kinase Regulates Cytoplasmic HuR
Wang, Wengong, Fan, Jinshui, Yang, Xiaoling, Fürer-Galban, Stefanie, Lopez De Silanes, Isabel, Von Kobbe, Cayetano, ...
While transport of RNA-binding protein HuR from nucleus to cytoplasm is emerging as a key regulatory step for HuR function, the mechanisms underlying this process remain poorly understood. Here, we...
Negative Feedback Regulation of MKK6 mRNA Stability by p38α Mitogen-Activated Protein Kinase
Ambrosino, Concetta, Mace, Gaetane, Galban, Stefanie, Fritsch, Cornelius, Vintersten, Kristina, Black, Emma, ...
p38 mitogen-activated protein (MAP) kinases play an important role in the regulation of cellular responses to all kinds of stresses. The most abundant and broadly expressed p38 MAP kinase is p38α,...
Shack, Sonsoles, Wang, Xian-Tao, Kokkonen, Gertrude C., Gorospe, Myriam, Longo, Dan L., Holbrook, Nikki J.
The inhibitory effect of caveolin on the cellular response to growth factor stimulation is well established. Given the significant overlap in signaling pathways involved in regulating cell...
Galbán, Stefanie, Fan, Jinshui, Martindale, Jennifer L., Cheadle, Chris, Hoffman, Bryan, Woods, Michael P., ...
Based on evidence that the von Hippel-Lindau (VHL) tumor suppressor protein is associated with polysomes and interacts with translation regulatory factors, we set out to investigate the potential...
Role of HuR in Skeletal Myogenesis through Coordinate Regulation of Muscle Differentiation Genes
Figueroa, Angélica, Cuadrado, Ana, Fan, Jinshui, Atasoy, Ulus, Muscat, George E., Muñoz-Canoves, Pura, ...
In this report, we investigate the role of the RNA-binding protein HuR during skeletal myogenesis. At the onset of myogenesis in differentiating C2C12 myocytes and in vivo in regenerating mouse...
RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation
Mazan-Mamczarz, Krystyna, Galbán, Stefanie, De Silanes, Isabel López, Martindale, Jennifer L., Atasoy, Ulus, Keene, Jack D., ...
Exposure to short-wavelength UV light (UVC) strongly induces p53 expression. In human RKO colorectal carcinoma cells, this increase was not due to elevated p53 mRNA abundance, cytoplasmic export of...
Influence of the RNA-Binding Protein HuR in pVHL-Regulated p53 Expression in Renal Carcinoma Cells
Galbán, Stefanie, Martindale, Jennifer L., Mazan-Mamczarz, Krystyna, De Silanes, Isabel López, Fan, Jinshui, Wang, Wengong, ...
A recent analysis of gene expression in renal cell carcinoma cells led to the identification of mRNAs whose translation was dependent on the presence of the von Hippel-Lindau (VHL) tumor suppressor...
Identification of a target RNA motif for RNA-binding protein HuR
De Silanes, Isabel López, Zhan, Ming, Lal, Ashish, Yang, Xiaoling, Gorospe, Myriam
HuR, a protein that binds to specific mRNA subsets, is increasingly recognized as a pivotal posttranscriptional regulator of gene expression. Here, HuR was immunoprecipitated under conditions that...
HuR regulates cyclin A and cyclin B1 mRNA stability during cell proliferation
Wang, Wengong, Caldwell, M.Craig, Lin, Shankung, Furneaux, Henry, Gorospe, Myriam
Colorectal carcinoma RKO cells expressing reduced levels of the RNA-binding protein HuR (ASHuR) displayed markedly reduced growth. In synchronous RKO populations, HuR was almost exclusively nuclear...
Kawai, Tomoko, Fan, Jinshui, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
The stability of mRNAs undergoing translation has long been a controversial question. Here, we systematically investigate links between mRNA turnover and translation during the endoplasmic reticulum...
Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs
Lal, Ashish, Mazan-Mamczarz, Krystyna, Kawai, Tomoko, Yang, Xiaoling, Martindale, Jennifer L, Gorospe, Myriam
RNA-binding proteins HuR and AUF1 bind to many common AU-rich target mRNAs and exert opposing influence on target mRNA stability, but the functional interactions between HuR and AUF1 have not been...
Antiapoptotic function of RNA-binding protein HuR effected through prothymosin α
Lal, Ashish, Kawai, Tomoko, Yang, Xiaoling, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
We report the antiapoptotic effect of RNA-binding protein HuR, a critical regulator of the post-transcriptional fate of target transcripts. Among the most prominent mRNAs complexing with HuR is that...
Identification and Functional Outcome of mRNAs Associated with RNA-Binding Protein TIA-1
López De Silanes, Isabel, Galbán, Stefanie, Martindale, Jennifer L., Yang, Xiaoling, Mazan-Mamczarz, Krystyna, Indig, Fred E., ...
The RNA-binding protein TIA-1 (T-cell intracellular antigen 1) functions as a posttranscriptional regulator of gene expression and aggregates to form stress granules following cellular damage. TIA-1...
Increased stability of the p16 mRNA with replicative senescence
Wang, Wengong, Martindale, Jennifer L, Yang, Xiaoling, Chrest, Francis J, Gorospe, Myriam
Expression of p16INK4a is elevated during ageing and replicative senescence. Here, we report the presence of an instability determinant within the 3′-untranslated region (UTR) of the p16 messenger...
Clean western blot signals from immunoprecipitated samples
Lal, Ashish, Haynes, Susan R., Gorospe, Myriam
We present a strategy that overcomes the high background arising during Western blotting (WB) detection of proteins obtained through immunoprecipitation (IP). Traditional HRP-conjugated secondary...
Pullmann, Rudolf, Juhaszova, Magdalena, Silanes, Isabel López De, Kawai, Tomoko, Mazan-Mamczarz, Krystyna, Halushka, Marc K., ...
In dividing cells, the RNA-binding protein HuR associates with and stabilizes labile mRNAs encoding proliferative proteins, events that are linked to the increased cytoplasmic presence of HuR. Here,...
En masse analysis of nascent translation using microarrays
Mazan-Mamczarz, Krystyna, Kawai, Tomoko, Martindale, Jennifer L., Gorospe, Myriam
We report a robust method for studying en masse changes in translation using cDNA arrays. The relative distribution of messenger RNAs (mRNAs) along polysome gradients was monitored by performing cDNA...
En masse nascent transcription analysis to elucidate regulatory transcription factors
Fan, Jinshui, Zhan, Ming, Shen, Jikui, Martindale, Jennifer L., Yang, Xiaoling, Kawai, Tomoko, ...
Despite exhaustively informing about steady-state mRNA abundance, DNA microarrays have been used with limited success to identify regulatory transcription factors (TFs). The main limitation of this...
Translational Repression by RNA-Binding Protein TIAR†
Mazan-Mamczarz, Krystyna, Lal, Ashish, Martindale, Jennifer L., Kawai, Tomoko, Gorospe, Myriam
The RNA-binding protein TIAR has been proposed to inhibit protein synthesis transiently by promoting the formation of translationally silent stress granules. Here, we report the selective binding of...
Translational Control of Cytochrome c by RNA-Binding Proteins TIA-1 and HuR
Kawai, Tomoko, Lal, Ashish, Yang, Xiaoling, Galban, Stefanie, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
Stresses affecting the endoplasmic reticulum (ER) globally modulate gene expression patterns by altering posttranscriptional processes such as translation. Here, we use tunicamycin (Tn) to...
HuR Regulates p21 mRNA Stabilization by UV Light
Wang, Wengong, Furneaux, Henry, Cheng, Huiming, Caldwell, M. Craig, Hutter, Dorothy, Liu, Yusen, ...
Expression of the cyclin-dependent kinase inhibitor p21 is highly induced by many stresses, including exposure to short-wavelength UV light (UVC), which increases p21 mRNA stability. Investigation...
Potapova, Olga, Gorospe, Myriam, Dougherty, Ryan H., Dean, Nicholas M., Gaarde, William A., Holbrook, Nikki J.
c-Jun N-terminal kinase (JNK) plays a critical role in coordinating the cellular response to stress and has been implicated in regulating cell growth and transformation. To investigate the...
Lin, Shankung, Wang, Wengong, Wilson, Gerald M., Yang, Xiaoling, Brewer, Gary, Holbrook, Nikki J., ...
Prostaglandin A2 (PGA2), an experimental chemotherapeutic agent, causes growth arrest associated with decreased cyclin D1 expression in several cancer cell lines. Here, using human non-small-cell...
Loss of HuR Is Linked to Reduced Expression of Proliferative Genes during Replicative Senescence
Wang, Wengong, Yang, Xiaoling, Cristofalo, Vincent J., Holbrook, Nikki J., Gorospe, Myriam
Cellular aging is accompanied by alterations in gene expression patterns. Here, using two models of replicative senescence, we describe the influence of the RNA-binding protein HuR in regulating the...
Li, Ji, Gorospe, Myriam, Hutter, Dorothy, Barnes, Janice, Keyse, Stephen M., Liu, Yusen
Mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) has been shown to play a critical role in mediating the feedback control of MAP kinase cascades in a variety of cellular processes,...
Gorospe, Myriam, Wang, Xiantao, Holbrook, Nikki J.
Exposure of mammalian cells to adverse stimuli triggers the expression of numerous stress response genes, many of which are presumed to enhance cell survival. In this study, we examined the...
Gorospe, Myriam, Egan, Josephine M., Zbar, Berton, Lerman, Michael, Geil, Laura, Kuzmin, Igor, ...
The absence of functional von Hippel-Lindau (VHL) tumor suppressor gene leads to the development of neoplasias characteristic of VHL disease, including renal cell carcinoma (RCC). Here, we compared...
Global analysis of stress-regulated mRNA turnover by using cDNA arrays
Fan, Jinshui, Yang, Xiaoling, Wang, Wengong, Wood, William H., Becker, Kevin G., Gorospe, Myriam
cDNA array technology has proven to be a powerful way to monitor global changes in gene expression patterns. Here, we present an approach that extends the current utility of cDNA arrays to allow the...
AMP-Activated Kinase Regulates Cytoplasmic HuR
Wang, Wengong, Fan, Jinshui, Yang, Xiaoling, Fürer-Galban, Stefanie, Lopez De Silanes, Isabel, Von Kobbe, Cayetano, ...
While transport of RNA-binding protein HuR from nucleus to cytoplasm is emerging as a key regulatory step for HuR function, the mechanisms underlying this process remain poorly understood. Here, we...
Negative Feedback Regulation of MKK6 mRNA Stability by p38α Mitogen-Activated Protein Kinase
Ambrosino, Concetta, Mace, Gaetane, Galban, Stefanie, Fritsch, Cornelius, Vintersten, Kristina, Black, Emma, ...
p38 mitogen-activated protein (MAP) kinases play an important role in the regulation of cellular responses to all kinds of stresses. The most abundant and broadly expressed p38 MAP kinase is p38α,...
Shack, Sonsoles, Wang, Xian-Tao, Kokkonen, Gertrude C., Gorospe, Myriam, Longo, Dan L., Holbrook, Nikki J.
The inhibitory effect of caveolin on the cellular response to growth factor stimulation is well established. Given the significant overlap in signaling pathways involved in regulating cell...
Galbán, Stefanie, Fan, Jinshui, Martindale, Jennifer L., Cheadle, Chris, Hoffman, Bryan, Woods, Michael P., ...
Based on evidence that the von Hippel-Lindau (VHL) tumor suppressor protein is associated with polysomes and interacts with translation regulatory factors, we set out to investigate the potential...
Role of HuR in Skeletal Myogenesis through Coordinate Regulation of Muscle Differentiation Genes
Figueroa, Angélica, Cuadrado, Ana, Fan, Jinshui, Atasoy, Ulus, Muscat, George E., Muñoz-Canoves, Pura, ...
In this report, we investigate the role of the RNA-binding protein HuR during skeletal myogenesis. At the onset of myogenesis in differentiating C2C12 myocytes and in vivo in regenerating mouse...
RNA-binding protein HuR enhances p53 translation in response to ultraviolet light irradiation
Mazan-Mamczarz, Krystyna, Galbán, Stefanie, De Silanes, Isabel López, Martindale, Jennifer L., Atasoy, Ulus, Keene, Jack D., ...
Exposure to short-wavelength UV light (UVC) strongly induces p53 expression. In human RKO colorectal carcinoma cells, this increase was not due to elevated p53 mRNA abundance, cytoplasmic export of...
Influence of the RNA-Binding Protein HuR in pVHL-Regulated p53 Expression in Renal Carcinoma Cells
Galbán, Stefanie, Martindale, Jennifer L., Mazan-Mamczarz, Krystyna, De Silanes, Isabel López, Fan, Jinshui, Wang, Wengong, ...
A recent analysis of gene expression in renal cell carcinoma cells led to the identification of mRNAs whose translation was dependent on the presence of the von Hippel-Lindau (VHL) tumor suppressor...
Identification of a target RNA motif for RNA-binding protein HuR
De Silanes, Isabel López, Zhan, Ming, Lal, Ashish, Yang, Xiaoling, Gorospe, Myriam
HuR, a protein that binds to specific mRNA subsets, is increasingly recognized as a pivotal posttranscriptional regulator of gene expression. Here, HuR was immunoprecipitated under conditions that...
HuR regulates cyclin A and cyclin B1 mRNA stability during cell proliferation
Wang, Wengong, Caldwell, M.Craig, Lin, Shankung, Furneaux, Henry, Gorospe, Myriam
Colorectal carcinoma RKO cells expressing reduced levels of the RNA-binding protein HuR (ASHuR) displayed markedly reduced growth. In synchronous RKO populations, HuR was almost exclusively nuclear...
Kawai, Tomoko, Fan, Jinshui, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
The stability of mRNAs undergoing translation has long been a controversial question. Here, we systematically investigate links between mRNA turnover and translation during the endoplasmic reticulum...
Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs
Lal, Ashish, Mazan-Mamczarz, Krystyna, Kawai, Tomoko, Yang, Xiaoling, Martindale, Jennifer L, Gorospe, Myriam
RNA-binding proteins HuR and AUF1 bind to many common AU-rich target mRNAs and exert opposing influence on target mRNA stability, but the functional interactions between HuR and AUF1 have not been...
Antiapoptotic function of RNA-binding protein HuR effected through prothymosin α
Lal, Ashish, Kawai, Tomoko, Yang, Xiaoling, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
We report the antiapoptotic effect of RNA-binding protein HuR, a critical regulator of the post-transcriptional fate of target transcripts. Among the most prominent mRNAs complexing with HuR is that...
Identification and Functional Outcome of mRNAs Associated with RNA-Binding Protein TIA-1
López De Silanes, Isabel, Galbán, Stefanie, Martindale, Jennifer L., Yang, Xiaoling, Mazan-Mamczarz, Krystyna, Indig, Fred E., ...
The RNA-binding protein TIA-1 (T-cell intracellular antigen 1) functions as a posttranscriptional regulator of gene expression and aggregates to form stress granules following cellular damage. TIA-1...
Increased stability of the p16 mRNA with replicative senescence
Wang, Wengong, Martindale, Jennifer L, Yang, Xiaoling, Chrest, Francis J, Gorospe, Myriam
Expression of p16INK4a is elevated during ageing and replicative senescence. Here, we report the presence of an instability determinant within the 3′-untranslated region (UTR) of the p16 messenger...
En masse nascent transcription analysis to elucidate regulatory transcription factors
Fan, Jinshui, Zhan, Ming, Shen, Jikui, Martindale, Jennifer L., Yang, Xiaoling, Kawai, Tomoko, ...
Despite exhaustively informing about steady-state mRNA abundance, DNA microarrays have been used with limited success to identify regulatory transcription factors (TFs). The main limitation of this...
Translational Repression by RNA-Binding Protein TIAR†
Mazan-Mamczarz, Krystyna, Lal, Ashish, Martindale, Jennifer L., Kawai, Tomoko, Gorospe, Myriam
The RNA-binding protein TIAR has been proposed to inhibit protein synthesis transiently by promoting the formation of translationally silent stress granules. Here, we report the selective binding of...
Translational Control of Cytochrome c by RNA-Binding Proteins TIA-1 and HuR
Kawai, Tomoko, Lal, Ashish, Yang, Xiaoling, Galban, Stefanie, Mazan-Mamczarz, Krystyna, Gorospe, Myriam
Stresses affecting the endoplasmic reticulum (ER) globally modulate gene expression patterns by altering posttranscriptional processes such as translation. Here, we use tunicamycin (Tn) to...
AGEMAP: A Gene Expression Database for Aging in Mice
Zahn, Jacob M, Poosala, Suresh, Owen, Art B, Ingram, Donald K, Lustig, Ana, Carter, Arnell, ...
We present the AGEMAP (Atlas of Gene Expression in Mouse Aging Project) gene expression database, which is a resource that catalogs changes in gene expression as a function of age in mice. The AGEMAP...
Xiao, Lan, Rao, Jaladanki N., Zou, Tongtong, Liu, Lan, Marasa, Bernard S., Chen, Jie, ...
Maintenance of intestinal mucosal epithelial integrity requires polyamines that modulate the expression of various genes involved in cell proliferation and apoptosis. Recently, polyamines were shown...
Elucidation of a C-Rich Signature Motif in Target mRNAs of RNA-Binding Protein TIAR▿ †
Kim, Henry S., Kuwano, Yuki, Zhan, Ming, Pullmann, Rudolf, Mazan-Mamczarz, Krystyna, Li, Huai, ...
The RNA-binding protein TIAR (related to TIA-1 [T-cell-restricted intracellular antigen 1]) was shown to associate with subsets of mRNAs bearing U-rich sequences in their 3′ untranslated regions....
Pullmann, Rudolf, Kim, Hyeon Ho, Abdelmohsen, Kotb, Lal, Ashish, Martindale, Jennifer L., Yang, Xiaoling, ...
RNA-binding proteins (RBPs) that associate with specific mRNA sequences and function as mRNA turnover and translation regulatory (TTR) RBPs are emerging as pivotal posttranscriptional regulators of...
Phosphorylation of p66Shc and forkhead proteins mediates Aβ toxicity
Smith, Wanli W., Norton, Darrell D., Gorospe, Myriam, Jiang, Haibing, Nemoto, Shino, Holbrook, Nikki J., ...
Excessive accumulation of amyloid β-peptide (Aβ) plays an early and critical role in synapse and neuronal loss in Alzheimer's Disease (AD). Increased oxidative stress is one of the mechanisms...
p16INK4a Translation Suppressed by miR-24
Lal, Ashish, Kim, Hyeon Ho, Abdelmohsen, Kotb, Kuwano, Yuki, Pullmann, Rudolf, Srikantan, Subramanya, ...
RNA-Binding Proteins HuR and PTB Promote the Translation of Hypoxia-Inducible Factor 1α▿ †
Galbán, Stefanie, Kuwano, Yuki, Pullmann, Rudolf, Martindale, Jennifer L., Kim, Hyeon Ho, Lal, Ashish, ...
The levels of hypoxia-inducible factor 1α (HIF-1α) are tightly controlled. Here, we investigated the posttranscriptional regulation of HIF-1α expression in human cervical carcinoma HeLa cells...
Chen, Jie, Xiao, Lan, Rao, Jaladanki N., Zou, Tongtong, Liu, Lan, Bellavance, Emily, ...
The AP-1 transcription factor JunD is highly expressed in intestinal epithelial cells, but its exact role in maintaining the integrity of intestinal epithelial barrier remains unknown. The tight...
MKP-1 mRNA Stabilization and Translational Control by RNA-Binding Proteins HuR and NF90▿ †
Kuwano, Yuki, Kim, Hyeon Ho, Abdelmohsen, Kotb, Pullmann, Rudolf, Martindale, Jennifer L., Yang, Xiaoling, ...
The mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) plays a major role in dephosphorylating and thereby inactivating the MAP kinases extracellular signal-regulated kinase (ERK), c-Jun...
Nuclear HuR accumulation through phosphorylation by Cdk1
Kim, Hyeon Ho, Abdelmohsen, Kotb, Lal, Ashish, Pullmann, Rudolf, Yang, Xiaoling, Galban, Stefanie, ...
A predominantly nuclear RNA-binding protein, HuR translocates to the cytoplasm in response to stress and proliferative signals, where it stabilizes or modulates the translation of target mRNAs. Here,...
Van Der Brug, Marcel P., Blackinton, Jeff, Chandran, Jayanth, Hao, Ling-Yang, Lal, Ashish, Mazan-Mamczarz, Krystyna, ...
Parkinson's disease (PD) is a major neurodegenerative condition with several rare Mendelian forms. Oxidative stress and mitochondrial function have been implicated in the pathogenesis of PD but the...
Identification of a signature motif in target mRNAs of RNA-binding protein AUF1
Mazan-Mamczarz, Krystyna, Kuwano, Yuki, Zhan, Ming, White, Elizabeth J., Martindale, Jennifer L., Lal, Ashish, ...
The ubiquitous RNA-binding protein AUF1 promotes the degradation of some target mRNAs, but increases the stability and translation of other targets. Here, we isolated AUF1-associated mRNAs by...
The RNA-binding protein HuR regulates DNA methylation through stabilization of DNMT3b mRNA
López De Silanes, Isabel, Gorospe, Myriam, Taniguchi, Hiroaki, Abdelmohsen, Kotb, Srikantan, Subramanya, Alaminos, Miguel, ...
The molecular basis underlying the aberrant DNA-methylation patterns in human cancer is largely unknown. Altered DNA methyltransferase (DNMT) activity is believed to contribute, as DNMT expression...
miR-519 reduces cell proliferation by lowering RNA-binding protein HuR levels
Abdelmohsen, Kotb, Srikantan, Subramanya, Kuwano, Yuki, Gorospe, Myriam
Gene expression is potently regulated through the action of RNA-binding proteins (RBPs) and microRNAs (miRNAs). Here, we present evidence of a miRNA regulating an RBP. The RBP HuR can stabilize and...
Novel Roles of Hakai in Cell Proliferation and Oncogenesis
Figueroa, Angélica, Kotani, Hirokazu, Toda, Yoshinobu, Mazan-Mamczarz, Krystyna, Mueller, Eva-Christina, Otto, Albrecht, ...
During tumor development, cells acquire multiple phenotypic changes upon misregulation of oncoproteins and tumor suppressor proteins. Hakai was originally identified as an E3 ubiquitin-ligase for the...
Kuwano, Yuki, Rabinovic, Ariel, Srikantan, Subramanya, Gorospe, Myriam, Demple, Bruce
We previously observed that nitric oxide (NO) exposure increases the stability of mRNAs encoding heme oxygenase 1 (HO-1) and TIEG-1 in human and mouse fibroblasts. Here, we have used microarrays to...
Stabilization of XIAP mRNA through the RNA binding protein HuR regulated by cellular polyamines
Zhang, Xian, Zou, Tongtong, Rao, Jaladanki N., Liu, Lan, Xiao, Lan, Wang, Peng-Yuan, ...
The X chromosome-linked inhibitor of apoptosis protein (XIAP) is the most potent intrinsic caspase inhibitor and plays an important role in the maintenance of intestinal epithelial integrity. The RNA...
NF90 selectively represses the translation of target mRNAs bearing an AU-rich signature motif
Kuwano, Yuki, Pullmann, Rudolf, Marasa, Bernard S., Abdelmohsen, Kotb, Lee, Eun Kyung, Yang, Xiaoling, ...
The RNA-binding protein nuclear factor 90 (NF90) has been implicated in the stabilization, transport and translational control of several target mRNAs. However, a systematic analysis of NF90 target...
HuR recruits let-7/RISC to repress c-Myc expression
Kim, Hyeon Ho, Kuwano, Yuki, Srikantan, Subramanya, Lee, Eun Kyung, Martindale, Jennifer L., Gorospe, Myriam
RNA-binding proteins (RBPs) and microRNAs (miRNAs) are potent post-transcriptional regulators of gene expression. Here, we show that the RBP HuR reduced c-Myc expression by associating with the c-Myc...