Wizemann, T M, Moskovitz, J, Pearce, B J, Cundell, D, Arvidson, C G, So, M, ...
Pathogenic bacteria rely on adhesins to bind to host tissues. Therefore, the maintenance of the functional properties of these extracellular macromolecules is essential for the pathogenicity of these...
Moskovitz, J, Jenkins, N A, Gilbert, D J, Copeland, N G, Jursky, F, Weissbach, H, ...
Peptide methionine sulfoxide reductase (MsrA; EC 1.8.4.6) is a ubiquitous protein that can reduce methionine sulfoxide residues in proteins as well as in a large number of methyl sulfoxide compounds....
Moskovitz, J, Weissbach, H, Brot, N
An enzyme that reduces methionine sulfoxide [Met(O)] residues in proteins [peptide Met(O) reductase (MsrA), EC 1.8.4.6; originally identified in Escherichia coli] was purified from bovine liver, and...
Similarity of nucleotide interactions of BiP and GTP-binding proteins.
Brot, N, Redfield, B, Qiu, N H, Chen, G J, Vidal, V, Carlino, A, ...
BiP is a member of the Hsp70 heat shock protein family found in the lumen of the endoplasmic reticulum, that binds to a variety of proteins destined to be secreted. Substance P (SP) has been used as...
Dissociation of glucose-regulated protein Grp78 and Grp78-IgE Fc complexes by ATP.
Toledo, H, Carlino, A, Vidal, V, Redfield, B, Nettleton, M Y, Kochan, J P, ...
Recent studies have shown that ATP can dissociate dimers of the glucose-regulated protein Grp78 to monomers. In the present study, we have used purified recombinant Grp78 from Escherichia coli to...
Drappa, J, Brot, N, Elkon, K B
The lymphoproliferation (lpr) mutation in the MRL strain of mice is caused by the insertion of the early transposable element ETn in the Fas gene. The insertion causes a striking decrease in Fas mRNA...
Carlino, A, Toledo, H, Skaleris, D, DeLisio, R, Weissbach, H, Brot, N
The hamster gene encoding the 78-kDa glucose-regulated protein (Grp78) was expressed in Escherichia coli as a fusion protein with glutathione S-transferase. After induction with isopropyl...
Amir-Shapira, D, Leustek, T, Dalie, B, Weissbach, H, Brot, N
The heat-shock response of Euglena gracilis was studied by pulse-labeling cells with [35S]sulfate at both the normal growth temperature (21 degrees C) and an elevated temperature (36 degrees C)....
Maxon, M E, Wigboldus, J, Brot, N, Weissbach, H
The Escherichia coli metR gene has been sequenced. The sequence predicts a protein of 317 amino acids and a calculated molecular weight of 35,628. This is about 15% larger than the protein from...
Moskovitz, J, Rahman, M A, Strassman, J, Yancey, S O, Kushner, S R, Brot, N, ...
The Escherichia coli peptide methionine sulfoxide reductase gene (msrA) encodes a single-subunit polypeptide of 212 amino acid residues (M. A. Rahman, H. Nelson, H. Weissbach, and N. Brot, J. Biol....
Heat shock-dependent transcriptional activation of the metA gene of Escherichia coli.
Biran, D, Brot, N, Weissbach, H, Ron, E Z
In Escherichia coli, the growth rate at elevated temperatures is controlled by the availability of endogenous methionine, which is limited because of the temperature sensitivity of the metA gene...
Leustek, T, Hartwig, R, Weissbach, H, Brot, N
The synthesis of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase) in Rhodospirillum rubrum was regulated by the CO2 concentration in the culture medium. The specific activity of RuBPCase in...
Bloom, M, Skelly, S, VanBogelen, R, Neidhardt, F, Brot, N, Weissbach, H
In Escherichia coli, the ability to elicit a heat shock response depends on the htpR gene product. Previous work has shown that the HtpR protein serves as a sigma factor (sigma 32) for RNA polymerase...
Reduction of methionine sulfoxide to methionine by Escherichia coli.
Ejiri, S I, Weissbach, H, Brot, N
L-Methionine-dl-sulfoxide can support the growth of an Escherichia coli methionine auxotroph, suggesting the presence of an enzyme(s) capable of reducing the sulfoxide to methionine. This was...
Properties of the ribosomal P2 protein autoantigen are similar to those of foreign protein antigens.
Elkon, K, Bonfa, E, Llovet, R, Danho, W, Weissbach, H, Brot, N
Approximately 15% of patients with systemic lupus erythematosus have autoantibodies that bind to a shared epitope previously shown to be located on the carboxyl-terminal 22 amino acids of three 60S...
Skelly, S, Fu, C F, Dalie, B, Redfield, B, Coleman, T, Brot, N, ...
A polyclonal antibody to sigma 32, the heat shock sigma factor, has been used to show the presence of low levels of sigma 32 in Escherichia coli RNA polymerase preparations (E sigma 70), which...
Maxon, M E, Redfield, B, Cai, X Y, Shoeman, R, Fujita, K, Fisher, W, ...
A plasmid (pRSE562) containing the metE and metR genes of Escherichia coli was used to study the expression of these genes and the role of the MetR protein in regulating metE expression. DNA sequence...
Methionine synthesis in Escherichia coli: effect of the MetR protein on metE and metH expression.
Cai, X Y, Maxon, M E, Redfield, B, Glass, R, Brot, N, Weissbach, H
Studies by Urbanowski et al. [Urbanowski, M. L., Stauffer, L. T., Plamann, L. S. & Stauffer, G. V. (1987) J. Bacteriol. 169, 1391-1397] have identified a regulatory locus, called metR, required for...
Crow, M K, DelGiudice-Asch, G, Zehetbauer, J B, Lawson, J L, Brot, N, Weissbach, H, ...
A role for helper T cells in the induction of pathogenic lupus autoantibodies is increasingly supported by data from studies of murine lupus and patients with systemic lupus erythematosus (SLE)....
A member of the Hsp70 family is localized in mitochondria and resembles Escherichia coli DnaK.
Leustek, T, Dalie, B, Amir-Shapira, D, Brot, N, Weissbach, H
A 71-kDa protein (P71) with properties similar to those of the Escherichia coli heat shock protein DnaK has been found in extracts of HeLa cells. P71 was copurified by ATP-agarose affinity...
Skelly, S, Coleman, T, Fu, C F, Brot, N, Weissbach, H
S-30 extracts from Escherichia coli cells were used to express heat shock (HS) and non-HS genes in vitro in a DNA-directed protein synthesis system. The S-30 extracts prepared from cells that have...
Enzymatic reduction of protein-bound methionine sulfoxide.
Brot, N, Weissbach, L, Werth, J, Weissbach, H
An enzyme that catalyzes the reduction of methionine sulfoxide residues in ribosomal protein L12 has been partially purified from Escherichia coli extracts. Methionine sulfoxide present in oxidize...
Cenatiempo, Y, Twardowski, T, Shoeman, R, Ernst, H, Brot, N, Weissbach, H, ...
Reovirus mRNAs directed the synthesis of fMet dipeptides in a translation initiation system reconstituted from rabbit reticulocyte initiation and elongation factors, Artemia salina 80S ribosomes,...
Cenatiempo, Y, Robakis, N, Meza-Basso, L, Brot, N, Weissbach, H, Reid, B R
A simplified translation system coupled to DNA transcription that involves assaying the synthesis of the first dipeptide of a gene product has been described recently [Robakis, N., Meza-Basso, L.,...
In vitro synthesis of the first dipeptide of the beta subunit of Escherichia coli RNA polymerase.
Peacock, S, Cenatiempo, Y, Robakis, N, Brot, N, Weissbach, H
Plasmids pNF1337 and pNF1341, which contain part of the L10 operon including the RNA polymerase beta-subunit gene, have been used as templates in vitro to investigate expression of the beta-subunit...
Enzymatic reduction of oxidized alpha-1-proteinase inhibitor restores biological activity.
Abrams, W R, Weinbaum, G, Weissbach, L, Weissbach, H, Brot, N
The major serum inhibitor of proteolytic activity, alpha-1-proteinase inhibitor (alpha-1-PI), (or alpha-1-antitrypsin) can be readily inactivated by oxidation [Carp, H. & Janoff, A. (1978) Am. Rev....
Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro.
Brot, N, Caldwell, P, Weissbach, H
The DNA-dependent in vitro synthesis of Escherichia coli ribosomal protein L10 was inhibited when L10 was added to the protein-synthesizing incubations. Addition of L10 had little or no effect on the...
In vitro regulation of DNA-dependent synthesis of Escherichia coli ribosomal protein L12.
Goldberg, G, Caldwell, P, Weissbach, H, Brot, N
The DNA of the transducing phage lambdarifd18 contains, among others, the genes for the ribosomal proteins L11, L1, L10, and L12 and the beta and beta' subunits of RNA polymerase...
Elkon, K, Skelly, S, Parnassa, A, Moller, W, Danho, W, Weissbach, H, ...
The characteristics of eukaryotic ribosomal proteins P0, P1, and P2 (P proteins) and their antigenic determinants were studied using the sera of patients with systemic lupus erythematosus (SLE). P0,...
Oxidation of methionine residues in proteins of activated human neutrophils.
Fliss, H, Weissbach, H, Brot, N
A simple assay for the detection of 35S-labeled methionine sulfoxide residues in proteins is described. The assay, which is based on the ability of CNBr to react with methionine but not with...
Caldwell, P, Luk, D C, Weissbach, H, Brot, N
Oxidation of ribosomal protein L12 with hydrogen peroxide converts the three methionine residues to methionine sulfoxide. The oxidized protein has a decreased ability to bind to ribosomes, interact...
Cenatiempo, Y, Twardowski, T, Redfield, B, Reid, B R, Dauerman, H, Weissbach, H, ...
An in vitro system for measurement of rabbit globin mRNA translation has been developed based on the formation of the NH2-terminal dipeptide, fMet-Val. The basic components include a partially...
DNA-directed in vitro synthesis of proteins involved in bacterial transcription and translation.
Zarucki-Schulz, T, Jerez, C, Goldberg, G, Kung, H F, Huang, K H, Brot, N, ...
The in vitro synthesis of elongation factor (EF)-Tu (tufB), the beta beta' subunits of RNA polymerase, ribosomal proteins L10 and L12 directed by DNA from the transducing phage lambda rifd 18, EF-Tu...
DNA dependent synthesis of protein L12 from escherichia coli ribosomes, in vitro.
Chu, F, Kung, H F, Caldwell, P, Weissbach, H, Brot, N
The in vitro synthesis of ribosomal protein L12 has been obtained in a coupled system with DNA extracted from the transducing phage lambdarifd18 as template. In addition, a second protein (molecular...
mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu.
Chu, F, Caldwell, P, Weissbach, H, Brot, N
RNA extracted from growing Escherichia coli can direct the in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu when an E. coli system is used. The synthesized L12 can be...
Wizemann, T M, Moskovitz, J, Pearce, B J, Cundell, D, Arvidson, C G, So, M, ...
Pathogenic bacteria rely on adhesins to bind to host tissues. Therefore, the maintenance of the functional properties of these extracellular macromolecules is essential for the pathogenicity of these...
Moskovitz, J, Jenkins, N A, Gilbert, D J, Copeland, N G, Jursky, F, Weissbach, H, ...
Peptide methionine sulfoxide reductase (MsrA; EC 1.8.4.6) is a ubiquitous protein that can reduce methionine sulfoxide residues in proteins as well as in a large number of methyl sulfoxide compounds....
Moskovitz, J, Weissbach, H, Brot, N
An enzyme that reduces methionine sulfoxide [Met(O)] residues in proteins [peptide Met(O) reductase (MsrA), EC 1.8.4.6; originally identified in Escherichia coli] was purified from bovine liver, and...
Similarity of nucleotide interactions of BiP and GTP-binding proteins.
Brot, N, Redfield, B, Qiu, N H, Chen, G J, Vidal, V, Carlino, A, ...
BiP is a member of the Hsp70 heat shock protein family found in the lumen of the endoplasmic reticulum, that binds to a variety of proteins destined to be secreted. Substance P (SP) has been used as...
Dissociation of glucose-regulated protein Grp78 and Grp78-IgE Fc complexes by ATP.
Toledo, H, Carlino, A, Vidal, V, Redfield, B, Nettleton, M Y, Kochan, J P, ...
Recent studies have shown that ATP can dissociate dimers of the glucose-regulated protein Grp78 to monomers. In the present study, we have used purified recombinant Grp78 from Escherichia coli to...
Drappa, J, Brot, N, Elkon, K B
The lymphoproliferation (lpr) mutation in the MRL strain of mice is caused by the insertion of the early transposable element ETn in the Fas gene. The insertion causes a striking decrease in Fas mRNA...
Carlino, A, Toledo, H, Skaleris, D, DeLisio, R, Weissbach, H, Brot, N
The hamster gene encoding the 78-kDa glucose-regulated protein (Grp78) was expressed in Escherichia coli as a fusion protein with glutathione S-transferase. After induction with isopropyl...
Amir-Shapira, D, Leustek, T, Dalie, B, Weissbach, H, Brot, N
The heat-shock response of Euglena gracilis was studied by pulse-labeling cells with [35S]sulfate at both the normal growth temperature (21 degrees C) and an elevated temperature (36 degrees C)....
Maxon, M E, Wigboldus, J, Brot, N, Weissbach, H
The Escherichia coli metR gene has been sequenced. The sequence predicts a protein of 317 amino acids and a calculated molecular weight of 35,628. This is about 15% larger than the protein from...
Moskovitz, J, Rahman, M A, Strassman, J, Yancey, S O, Kushner, S R, Brot, N, ...
The Escherichia coli peptide methionine sulfoxide reductase gene (msrA) encodes a single-subunit polypeptide of 212 amino acid residues (M. A. Rahman, H. Nelson, H. Weissbach, and N. Brot, J. Biol....
Heat shock-dependent transcriptional activation of the metA gene of Escherichia coli.
Biran, D, Brot, N, Weissbach, H, Ron, E Z
In Escherichia coli, the growth rate at elevated temperatures is controlled by the availability of endogenous methionine, which is limited because of the temperature sensitivity of the metA gene...
Leustek, T, Hartwig, R, Weissbach, H, Brot, N
The synthesis of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCase) in Rhodospirillum rubrum was regulated by the CO2 concentration in the culture medium. The specific activity of RuBPCase in...
Bloom, M, Skelly, S, VanBogelen, R, Neidhardt, F, Brot, N, Weissbach, H
In Escherichia coli, the ability to elicit a heat shock response depends on the htpR gene product. Previous work has shown that the HtpR protein serves as a sigma factor (sigma 32) for RNA polymerase...
Reduction of methionine sulfoxide to methionine by Escherichia coli.
Ejiri, S I, Weissbach, H, Brot, N
L-Methionine-dl-sulfoxide can support the growth of an Escherichia coli methionine auxotroph, suggesting the presence of an enzyme(s) capable of reducing the sulfoxide to methionine. This was...
Properties of the ribosomal P2 protein autoantigen are similar to those of foreign protein antigens.
Elkon, K, Bonfa, E, Llovet, R, Danho, W, Weissbach, H, Brot, N
Approximately 15% of patients with systemic lupus erythematosus have autoantibodies that bind to a shared epitope previously shown to be located on the carboxyl-terminal 22 amino acids of three 60S...
Skelly, S, Fu, C F, Dalie, B, Redfield, B, Coleman, T, Brot, N, ...
A polyclonal antibody to sigma 32, the heat shock sigma factor, has been used to show the presence of low levels of sigma 32 in Escherichia coli RNA polymerase preparations (E sigma 70), which...
Maxon, M E, Redfield, B, Cai, X Y, Shoeman, R, Fujita, K, Fisher, W, ...
A plasmid (pRSE562) containing the metE and metR genes of Escherichia coli was used to study the expression of these genes and the role of the MetR protein in regulating metE expression. DNA sequence...
Methionine synthesis in Escherichia coli: effect of the MetR protein on metE and metH expression.
Cai, X Y, Maxon, M E, Redfield, B, Glass, R, Brot, N, Weissbach, H
Studies by Urbanowski et al. [Urbanowski, M. L., Stauffer, L. T., Plamann, L. S. & Stauffer, G. V. (1987) J. Bacteriol. 169, 1391-1397] have identified a regulatory locus, called metR, required for...
Crow, M K, DelGiudice-Asch, G, Zehetbauer, J B, Lawson, J L, Brot, N, Weissbach, H, ...
A role for helper T cells in the induction of pathogenic lupus autoantibodies is increasingly supported by data from studies of murine lupus and patients with systemic lupus erythematosus (SLE)....
A member of the Hsp70 family is localized in mitochondria and resembles Escherichia coli DnaK.
Leustek, T, Dalie, B, Amir-Shapira, D, Brot, N, Weissbach, H
A 71-kDa protein (P71) with properties similar to those of the Escherichia coli heat shock protein DnaK has been found in extracts of HeLa cells. P71 was copurified by ATP-agarose affinity...
Skelly, S, Coleman, T, Fu, C F, Brot, N, Weissbach, H
S-30 extracts from Escherichia coli cells were used to express heat shock (HS) and non-HS genes in vitro in a DNA-directed protein synthesis system. The S-30 extracts prepared from cells that have...
Enzymatic reduction of protein-bound methionine sulfoxide.
Brot, N, Weissbach, L, Werth, J, Weissbach, H
An enzyme that catalyzes the reduction of methionine sulfoxide residues in ribosomal protein L12 has been partially purified from Escherichia coli extracts. Methionine sulfoxide present in oxidize...
Cenatiempo, Y, Twardowski, T, Shoeman, R, Ernst, H, Brot, N, Weissbach, H, ...
Reovirus mRNAs directed the synthesis of fMet dipeptides in a translation initiation system reconstituted from rabbit reticulocyte initiation and elongation factors, Artemia salina 80S ribosomes,...
Cenatiempo, Y, Robakis, N, Meza-Basso, L, Brot, N, Weissbach, H, Reid, B R
A simplified translation system coupled to DNA transcription that involves assaying the synthesis of the first dipeptide of a gene product has been described recently [Robakis, N., Meza-Basso, L.,...
In vitro synthesis of the first dipeptide of the beta subunit of Escherichia coli RNA polymerase.
Peacock, S, Cenatiempo, Y, Robakis, N, Brot, N, Weissbach, H
Plasmids pNF1337 and pNF1341, which contain part of the L10 operon including the RNA polymerase beta-subunit gene, have been used as templates in vitro to investigate expression of the beta-subunit...