N. Furuya

Direct evidence for antipseudomonal activity of macrolides: exposure-dependent bactericidal activity and inhibition of protein synthesis by erythromycin, clarithromycin, and azithromycin.

Tateda, K, Ishii, Y, Matsumoto, T, Furuya, N, Nagashima, M, Matsunaga, T, ...

Several previous investigators have reported that long-term administration of certain macrolides is efficacious in patients with persistent pulmonary Pseudomonas aeruginosa infections, even though...

Immunomodulating effect of fosfomycin on gut-derived sepsis caused by Pseudomonas aeruginosa in mice.

Matsumoto, T, Tateda, K, Miyazaki, S, Furuya, N, Ohno, A, Ishii, Y, ...

We evaluated the protective effect of fosfomycin (FOM) and an enantiomer of fosfomycin [FOM (+); an isomer of FOM with no bactericidal activity] on murine gut-derived sepsis caused by Pseudomonas...

In vitro and in vivo antibacterial activities of CS-940, a new fluoroquinolone, against isolates from patients with respiratory infections.

Miyazaki, S, Domon, H, Tateda, K, Ohno, A, Ishii, Y, Matsumoto, T, ...

We compared the in vivo and in vitro activities of CS-940, a new fluoroquinolone, with those of a group of other drugs. The activities of CS-940 against gram-positive cocci and gram-negative rods,...

Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64.

Furuya, N, Komano, T

Products of the nikA and nikB genes of plasmid R64 have been shown to form a relaxation complex with R64 oriT DNA and to function together as an oriT-specific nickase. We purified the protein product...

Nucleotide sequence and characterization of the trbABC region of the IncI1 Plasmid R64: existence of the pnd gene for plasmid maintenance within the transfer region.

Furuya, N, Komano, T

A 6.72-kb DNA sequence between the exc gene and the oriT operon within the transfer region of IncI1 plasmid R64 was sequenced and characterized. Three novel transfer genes, trbA, trbB, and trbC, were...

Mutational analysis of the R64 oriT region: requirement for precise location of the NikA-binding sequence.

Furuya, N, Komano, T

Conjugative DNA transfer of IncI1 plasmid R64 is initiated by the introduction of a site- and strand-specific nick into the origin of transfer (oriT). In R64 oriT, 17-bp (repeat A and B) and 8-bp...

Effects of sub-MICs of erythromycin and other macrolide antibiotics on serum sensitivity of Pseudomonas aeruginosa.

Tateda, K, Hirakata, Y, Furuya, N, Ohno, A, Yamaguchi, K

We examined the effects of sub-MICs of erythromycin (EM) and other macrolide antibiotics on the serum sensitivity of Pseudomonas aeruginosa. P. aeruginosa S-6 grown for 36 and 48 h on agar with 10...

Efficacy of erythromycin lactobionate for treating Pseudomonas aeruginosa bacteremia in mice.

Hirakata, Y, Kaku, M, Tomono, K, Tateda, K, Furuya, N, Matsumoto, T, ...

We induced endogenous Pseudomonas aeruginosa bacteremia by administering cyclophosphamide and ampicillin to specific pathogen-free mice fed P. aeruginosa. Using this model, we evaluated the efficacy...

Potential effects of erythromycin on host defense systems and virulence of Pseudomonas aeruginosa.

Hirakata, Y, Kaku, M, Mizukane, R, Ishida, K, Furuya, N, Matsumoto, T, ...

We evaluated several potential effects of erythromycin (EM) on host defense systems and the virulence of Pseudomonas aeruginosa. Peritoneal macrophages obtained from mice given 250 mg of EM per kg of...

Nucleotide sequence and functions of the oriT operon in IncI1 plasmid R64.

Furuya, N, Nisioka, T, Komano, T

Two transfer genes of IncI1 plasmid R64, tentatively designated nikA and nikB, were cloned and sequenced. They are located adjacent to the origin of transfer (oriT) and appear to be organized into an...

Determination of the nick site at oriT of IncI1 plasmid R64: global similarity of oriT structures of IncI1 and IncP plasmids.

Furuya, N, Komano, T

The nick site at the origin of transfer, oriT, of IncI1 plasmid R64 was determined. A site-specific and strand-specific cleavage of the phosphodiester bond was introduced during relaxation of the...

Role of bacterial association with Kupffer cells in occurrence of endogenous systemic bacteremia.

Hirakata, Y, Tomono, K, Tateda, K, Matsumoto, T, Furuya, N, Shimoguchi, K, ...

Bacteremia in immunocompromised hosts often arises from their endogenous intestinal flora. We produced experimental endogenous bacteremia by administering cyclophosphamide and ampicillin to...

In vivo production of exotoxin A and its role in endogenous Pseudomonas aeruginosa septicemia in mice.

Hirakata, Y, Furuya, N, Tateda, K, Kaku, M, Yamaguchi, K

We have examined the production of Pseudomonas aeruginosa exotoxin A (ETA) and its role in endogenous bacteremia in mice. Mice given P. aeruginosa D4 orally died of bacteremia between days 10 and 13...

Comparative in vitro exoenzyme-suppressing activities of azithromycin and other macrolide antibiotics against Pseudomonas aeruginosa.

Mizukane, R, Hirakata, Y, Kaku, M, Ishii, Y, Furuya, N, Ishida, K, ...

The inhibitory effects of azithromycin (AZM), a new 15-membered macrolide antibiotic, on the production of exotoxin A, total protease, elastase, and phospholipase C by Pseudomonas aeruginosa were...

Direct evidence for antipseudomonal activity of macrolides: exposure-dependent bactericidal activity and inhibition of protein synthesis by erythromycin, clarithromycin, and azithromycin.

Tateda, K, Ishii, Y, Matsumoto, T, Furuya, N, Nagashima, M, Matsunaga, T, ...

Several previous investigators have reported that long-term administration of certain macrolides is efficacious in patients with persistent pulmonary Pseudomonas aeruginosa infections, even though...

Immunomodulating effect of fosfomycin on gut-derived sepsis caused by Pseudomonas aeruginosa in mice.

Matsumoto, T, Tateda, K, Miyazaki, S, Furuya, N, Ohno, A, Ishii, Y, ...

We evaluated the protective effect of fosfomycin (FOM) and an enantiomer of fosfomycin [FOM (+); an isomer of FOM with no bactericidal activity] on murine gut-derived sepsis caused by Pseudomonas...

In vitro and in vivo antibacterial activities of CS-940, a new fluoroquinolone, against isolates from patients with respiratory infections.

Miyazaki, S, Domon, H, Tateda, K, Ohno, A, Ishii, Y, Matsumoto, T, ...

We compared the in vivo and in vitro activities of CS-940, a new fluoroquinolone, with those of a group of other drugs. The activities of CS-940 against gram-positive cocci and gram-negative rods,...

Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64.

Furuya, N, Komano, T

Products of the nikA and nikB genes of plasmid R64 have been shown to form a relaxation complex with R64 oriT DNA and to function together as an oriT-specific nickase. We purified the protein product...

Nucleotide sequence and characterization of the trbABC region of the IncI1 Plasmid R64: existence of the pnd gene for plasmid maintenance within the transfer region.

Furuya, N, Komano, T

A 6.72-kb DNA sequence between the exc gene and the oriT operon within the transfer region of IncI1 plasmid R64 was sequenced and characterized. Three novel transfer genes, trbA, trbB, and trbC, were...

Mutational analysis of the R64 oriT region: requirement for precise location of the NikA-binding sequence.

Furuya, N, Komano, T

Conjugative DNA transfer of IncI1 plasmid R64 is initiated by the introduction of a site- and strand-specific nick into the origin of transfer (oriT). In R64 oriT, 17-bp (repeat A and B) and 8-bp...

Effects of sub-MICs of erythromycin and other macrolide antibiotics on serum sensitivity of Pseudomonas aeruginosa.

Tateda, K, Hirakata, Y, Furuya, N, Ohno, A, Yamaguchi, K

We examined the effects of sub-MICs of erythromycin (EM) and other macrolide antibiotics on the serum sensitivity of Pseudomonas aeruginosa. P. aeruginosa S-6 grown for 36 and 48 h on agar with 10...

Efficacy of erythromycin lactobionate for treating Pseudomonas aeruginosa bacteremia in mice.

Hirakata, Y, Kaku, M, Tomono, K, Tateda, K, Furuya, N, Matsumoto, T, ...

We induced endogenous Pseudomonas aeruginosa bacteremia by administering cyclophosphamide and ampicillin to specific pathogen-free mice fed P. aeruginosa. Using this model, we evaluated the efficacy...

Potential effects of erythromycin on host defense systems and virulence of Pseudomonas aeruginosa.

Hirakata, Y, Kaku, M, Mizukane, R, Ishida, K, Furuya, N, Matsumoto, T, ...

We evaluated several potential effects of erythromycin (EM) on host defense systems and the virulence of Pseudomonas aeruginosa. Peritoneal macrophages obtained from mice given 250 mg of EM per kg of...

Nucleotide sequence and functions of the oriT operon in IncI1 plasmid R64.

Furuya, N, Nisioka, T, Komano, T

Two transfer genes of IncI1 plasmid R64, tentatively designated nikA and nikB, were cloned and sequenced. They are located adjacent to the origin of transfer (oriT) and appear to be organized into an...

Determination of the nick site at oriT of IncI1 plasmid R64: global similarity of oriT structures of IncI1 and IncP plasmids.

Furuya, N, Komano, T

The nick site at the origin of transfer, oriT, of IncI1 plasmid R64 was determined. A site-specific and strand-specific cleavage of the phosphodiester bond was introduced during relaxation of the...

Role of bacterial association with Kupffer cells in occurrence of endogenous systemic bacteremia.

Hirakata, Y, Tomono, K, Tateda, K, Matsumoto, T, Furuya, N, Shimoguchi, K, ...

Bacteremia in immunocompromised hosts often arises from their endogenous intestinal flora. We produced experimental endogenous bacteremia by administering cyclophosphamide and ampicillin to...

In vivo production of exotoxin A and its role in endogenous Pseudomonas aeruginosa septicemia in mice.

Hirakata, Y, Furuya, N, Tateda, K, Kaku, M, Yamaguchi, K

We have examined the production of Pseudomonas aeruginosa exotoxin A (ETA) and its role in endogenous bacteremia in mice. Mice given P. aeruginosa D4 orally died of bacteremia between days 10 and 13...

Comparative in vitro exoenzyme-suppressing activities of azithromycin and other macrolide antibiotics against Pseudomonas aeruginosa.

Mizukane, R, Hirakata, Y, Kaku, M, Ishii, Y, Furuya, N, Ishida, K, ...

The inhibitory effects of azithromycin (AZM), a new 15-membered macrolide antibiotic, on the production of exotoxin A, total protease, elastase, and phospholipase C by Pseudomonas aeruginosa were...