Berg, K.B., Carr, J.M., Clark, M.J., Cook, N.J., Anderson, N.G., Scott, N.J., ...
NAFLD, liver component of the “metabolic” syndrome, has become the most common liver disease in western nations. Non-invasive imaging techniques exist, but have limitations, especially in...
Contrast agent recognition in small animal CT using the Medipix2 detector (2009)
Firsching, M., Butler, A.P.H., Scott, N., Anderson, N.G., Michel, T., Anton, G., ...
Energy resolving capabilities of X-ray detectors like the Medipix2 and the upcoming Medipix3 offer access to spectral information which is a new domain of information in medical imaging. In...
Butler, A.P.H., Anderson, N.G., Hurrell, M.A., Cook, N.J., Scott, N.J., Butler, P.H.
Purpose: To establish that a spectroscopic (multi-energy) CT scanner can differentiate multiple contrast agents and background tissues. This is clinically significant because it enables multi-phase...
Bio-medical X-ray imaging with spectroscopic pixel detectors (2008)
Butler, A.P.H., Anderson, N.G., Tipples, R., Cook, N., Watts, R., Meyer, J., ...
The aim of this study is to review the clinical potential of spectroscopic X-ray detectors and to undertake a feasibility study using a novel detector in a clinical hospital setting. Detectors...
Respiratory Syncytial Virus Isolation by Combined Continuous Flow-Isopycnic Banding Centrifugation
Cline, G. B., Coates, Helen, Anderson, N. G., Chanock, R. M., Harris, W. W.
A new zonal centrifuge rotor (B-IX) which combines continuous sample flow centrifugation with isopycnic banding has been used to isolate and concentrate respiratory syncytial virus from liter volumes...
Purification of Large Quantities of Influenza Virus by Density Gradient Centrifugation
Reimer, C. B., Baker, R. S., VanFrank, R. M., Newlin, T. E., Cline, G. B., Anderson, N. G.
New zonal centrifuges can conveniently process as much as five orders of magnitude (105) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding...
Mass Purification of Nucleopolyhedrosis Virus Inclusion Bodies in the K-Series Centrifuge
Breillatt, J. P., Brantley, J. N., Mazzone, H. M., Martignoni, M. E., Franklin, J. E., Anderson, N. G.
Nucleopolyhedrosis virus inclusion bodies specific for Hemerocampa pseudotsugata, Neodiprion sertifer, Porthetria dispar, and Heliothis zea have been purified by using a...
High resolution two-dimensional electrophoresis of human plasma proteins.
The two-dimensional electrophoretic technique of O'Farrell has been adapted to the analysis of human plasma proteins, and 30 polypeptides have been identified in the pattern produced. Genetic...
Physiological concentrations of growth hormone induced a rapid and transient activation of mitogen-activated protein kinase (MAP kinase) and S6 kinase in 3T3-F442A preadipocytes. These effects were...
Subcellular localization and hormone sensitivity of adipocyte cyclic AMP phosphodiesterase.
Anderson, N G, Kilgour, E, Houslay, M D
Treatment of intact adipocytes with either or both insulin and adrenaline stimulated membrane cyclic AMP phosphodiesterase activity only in the endoplasmic reticulum subfraction. The cyclic...
Kilgour, E, Anderson, N G, Houslay, M D
Incubation of a hepatocyte particulate fraction with ATP and the isolated catalytic unit of cyclic AMP-dependent protein kinase (A-kinase) selectively activated the high-affinity 'dense-vesicle'...
Shaw, G P, Anderson, N G, Hanson, P J
The substrate-dependency of gastric acid secretion was investigated in isolated rat parietal cells by using the accumulation of the weak base aminopyrine as an index of acid secretion. Exogenous...
Action of epidermal growth factor on acid secretion by rat isolated parietal cells.
Shaw, G P, Hatt, J F, Anderson, N G, Hanson, P J
The site and mechanism of action of epidermal growth factor (EGF) on acid secretion by rat isolated parietal cells were investigated by using the intracellular accumulation of the weak base...
The relative potency with which phorbol esters inhibited histamine-stimulated aminopyrine accumulation (an index of acid secretion) paralleled that which has been established for the activation of...
Arteriovenous differences for several potential metabolic substrates were measured across the fundic wall of the stomach of rats that had been starved overnight. There was an uptake of glucose and...
Arteriovenous differences for amino acids across control and acid-secreting rat stomach in vivo.
1. A method is described for measuring arteriovenous differences across the rat stomach in vivo. 2. Notable results were the uptake of branched-chain amino acids, the uptake of arginine, which was...
Kilgour, E, Gout, I, Anderson, N G
Pituitary growth hormone (GH) co-ordinately stimulates three distinct signalling pathways in 3T3-F442A preadipocytes, the STAT (signal transducer and activator of transcription) pathway, the...
Burt, A R, Carr, I C, Mullaney, I, Anderson, N G, Milligan, G
Rat-1 fibroblasts were transfected with a cDNA encoding the mouse delta opioid receptor. Two separate clones, D2 (which expressed some 6 pmol of the receptor/mg of membrane protein) and DOE (which...
MacKenzie, S, Fleming, I, Houslay, M D, Anderson, N G, Kilgour, E
Previous studies have shown that the activation of p44 and p42 mitogen-activated protein (MAP) kinases (ERK1 and ERK2) by growth hormone (GH) and phorbol esters, but not by epidermal growth factor,...
Wilson, M A, Burt, A R, Milligan, G, Anderson, N G
The regulation of mitogenic signalling pathways by G-protein-coupled receptors has been studied in Rat-1 fibroblasts stably transfected with the murine delta opioid receptor. We showed recently that...
Protein kinase C isoforms play differential roles in the regulation of adipocyte differentiation.
Fleming, I, MacKenzie, S J, Vernon, R G, Anderson, N G, Houslay, M D, Kilgour, E
In this study we first established, by immunoblotting with specific antibodies, the temporal changes in cellular levels of protein kinase C (PKC) isoforms during differentiation of 3T3-F442A...
Raf-1 is a potential substrate for mitogen-activated protein kinase in vivo.
Anderson, N G, Li, P, Marsden, L A, Williams, N, Roberts, T M, Sturgill, T W
MAP (mitogen-activated protein) kinase is shown to phosphorylate baculovirally expressed Raf-1 in vitro, generating one major tryptic phosphopeptide which co-migrated with a peptide from Raf-1...
Zonal centrifuges may be loaded during rotation (dynamically) or at rest (statically). In the latter case, the shearing forces occurring in a liquid confined in a closed cylinder, during the...
Respiratory Syncytial Virus Isolation by Combined Continuous Flow-Isopycnic Banding Centrifugation
Cline, G. B., Coates, Helen, Anderson, N. G., Chanock, R. M., Harris, W. W.
A new zonal centrifuge rotor (B-IX) which combines continuous sample flow centrifugation with isopycnic banding has been used to isolate and concentrate respiratory syncytial virus from liter volumes...
Purification of Large Quantities of Influenza Virus by Density Gradient Centrifugation
Reimer, C. B., Baker, R. S., VanFrank, R. M., Newlin, T. E., Cline, G. B., Anderson, N. G.
New zonal centrifuges can conveniently process as much as five orders of magnitude (105) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding...
Mass Purification of Nucleopolyhedrosis Virus Inclusion Bodies in the K-Series Centrifuge
Breillatt, J. P., Brantley, J. N., Mazzone, H. M., Martignoni, M. E., Franklin, J. E., Anderson, N. G.
Nucleopolyhedrosis virus inclusion bodies specific for Hemerocampa pseudotsugata, Neodiprion sertifer, Porthetria dispar, and Heliothis zea have been purified by using a...
High resolution two-dimensional electrophoresis of human plasma proteins.
The two-dimensional electrophoretic technique of O'Farrell has been adapted to the analysis of human plasma proteins, and 30 polypeptides have been identified in the pattern produced. Genetic...
Physiological concentrations of growth hormone induced a rapid and transient activation of mitogen-activated protein kinase (MAP kinase) and S6 kinase in 3T3-F442A preadipocytes. These effects were...
Subcellular localization and hormone sensitivity of adipocyte cyclic AMP phosphodiesterase.
Anderson, N G, Kilgour, E, Houslay, M D
Treatment of intact adipocytes with either or both insulin and adrenaline stimulated membrane cyclic AMP phosphodiesterase activity only in the endoplasmic reticulum subfraction. The cyclic...
Kilgour, E, Anderson, N G, Houslay, M D
Incubation of a hepatocyte particulate fraction with ATP and the isolated catalytic unit of cyclic AMP-dependent protein kinase (A-kinase) selectively activated the high-affinity 'dense-vesicle'...
Shaw, G P, Anderson, N G, Hanson, P J
The substrate-dependency of gastric acid secretion was investigated in isolated rat parietal cells by using the accumulation of the weak base aminopyrine as an index of acid secretion. Exogenous...
Action of epidermal growth factor on acid secretion by rat isolated parietal cells.
Shaw, G P, Hatt, J F, Anderson, N G, Hanson, P J
The site and mechanism of action of epidermal growth factor (EGF) on acid secretion by rat isolated parietal cells were investigated by using the intracellular accumulation of the weak base...
Raf-1 is a potential substrate for mitogen-activated protein kinase in vivo.
Anderson, N G, Li, P, Marsden, L A, Williams, N, Roberts, T M, Sturgill, T W
MAP (mitogen-activated protein) kinase is shown to phosphorylate baculovirally expressed Raf-1 in vitro, generating one major tryptic phosphopeptide which co-migrated with a peptide from Raf-1...
The relative potency with which phorbol esters inhibited histamine-stimulated aminopyrine accumulation (an index of acid secretion) paralleled that which has been established for the activation of...
Arteriovenous differences for several potential metabolic substrates were measured across the fundic wall of the stomach of rats that had been starved overnight. There was an uptake of glucose and...
Arteriovenous differences for amino acids across control and acid-secreting rat stomach in vivo.
1. A method is described for measuring arteriovenous differences across the rat stomach in vivo. 2. Notable results were the uptake of branched-chain amino acids, the uptake of arginine, which was...
Kilgour, E, Gout, I, Anderson, N G
Pituitary growth hormone (GH) co-ordinately stimulates three distinct signalling pathways in 3T3-F442A preadipocytes, the STAT (signal transducer and activator of transcription) pathway, the...
Burt, A R, Carr, I C, Mullaney, I, Anderson, N G, Milligan, G
Rat-1 fibroblasts were transfected with a cDNA encoding the mouse delta opioid receptor. Two separate clones, D2 (which expressed some 6 pmol of the receptor/mg of membrane protein) and DOE (which...
MacKenzie, S, Fleming, I, Houslay, M D, Anderson, N G, Kilgour, E
Previous studies have shown that the activation of p44 and p42 mitogen-activated protein (MAP) kinases (ERK1 and ERK2) by growth hormone (GH) and phorbol esters, but not by epidermal growth factor,...
Wilson, M A, Burt, A R, Milligan, G, Anderson, N G
The regulation of mitogenic signalling pathways by G-protein-coupled receptors has been studied in Rat-1 fibroblasts stably transfected with the murine delta opioid receptor. We showed recently that...
Protein kinase C isoforms play differential roles in the regulation of adipocyte differentiation.
Fleming, I, MacKenzie, S J, Vernon, R G, Anderson, N G, Houslay, M D, Kilgour, E
In this study we first established, by immunoblotting with specific antibodies, the temporal changes in cellular levels of protein kinase C (PKC) isoforms during differentiation of 3T3-F442A...
Zonal centrifuges may be loaded during rotation (dynamically) or at rest (statically). In the latter case, the shearing forces occurring in a liquid confined in a closed cylinder, during the...
Isolation of Relaxing Particles from Rat Skeletal Muscles in Zonal Centrifuges
Schuel, H., Lorand, L., Schuel, R., Anderson, N. G.
Supernatants of rat skeletal muscle homogenates were fractionated by differential centrifugation and by zonal centrifugation in sucrose density gradients. Cytochrome oxidase was employed as an...