Miyamoto, N G, Moncollin, V, Wintzerith, M, Hen, R, Egly, J M, Chambon, P
We have previously reported that sequences located upstream from the TATA box of the Adenovirus-2 major late promoter (Ad2MLP), between -34 and -97, are necessary for efficient transcription in vivo...
Transcription factor binding and spacing constraints in the human beta-actin proximal promoter.
Danilition, S L, Frederickson, R M, Taylor, C Y, Miyamoto, N G
The human beta-actin promoter, including its 5' flanking region and 5' untranslated region, is ubiquitously active in mammalian cells in culture. In this report we investigated the transcriptional...
Frederickson, R M, Micheau, M R, Iwamoto, A, Miyamoto, N G
We have identified a CCAAT box element that is required for the efficient transcription of the human beta-actin gene. Both in vivo transient transfection assays in cultured HeLa cells and in vitro...
Point mutational analysis of the human c-fos serum response factor binding site.
A series of point mutants were generated in the human c-fos dyad symmetry element (DSE), found within the c-fos serum response element, to study the sequence requirements for its interaction with the...
Stimulation of transcription in vitro by binding sites for nuclear factor I.
Gronostajski, R M, Knox, J, Berry, D, Miyamoto, N G
Nuclear factor I (NFI) is a site-specific DNA binding protein required for the replication of adenovirus DNA in vitro and in vivo. We have examined the effect of natural and synthetic binding sites...
Takadera, T, Leung, S, Gernone, A, Koga, Y, Takihara, Y, Miyamoto, N G, ...
The human T-cell- or lymphocyte-specific gene, lck, encodes a tyrosine kinase and is a member of the src family. In this report we demonstrate that there are two classes of human lck transcripts...
Miyamoto, N G, Moncollin, V, Egly, J M, Chambon, P
Stimulation of in vitro transcription mediated by the upstream element of the adenovirus-2 major late promoter (Ad2MLP) involves its recognition by a specific trans-acting factor present in a HeLa...
Is actin a transcription initiation factor for RNA polymerase B?
Egly, J M, Miyamoto, N G, Moncollin, V, Chambon, P
We have previously reported that two fractions derived from HeLa cell S100 extracts, the heparin flow-through and the heparin 0.6 M KCl eluate are required in vitro for efficient and accurate...
Purification of a factor specific for the upstream element of the adenovirus-2 major late promoter.
Moncollin, V, Miyamoto, N G, Zheng, X M, Egly, J M
Stimulation of in vitro transcription by the upstream element (UE) of the adenovirus-2 major late promoter (Ad2MLP) involves a specific trans-acting factor present in a HeLa whole-cell extract. By...
Miyamoto, N G, Moncollin, V, Wintzerith, M, Hen, R, Egly, J M, Chambon, P
We have previously reported that sequences located upstream from the TATA box of the Adenovirus-2 major late promoter (Ad2MLP), between -34 and -97, are necessary for efficient transcription in vivo...
Transcription factor binding and spacing constraints in the human beta-actin proximal promoter.
Danilition, S L, Frederickson, R M, Taylor, C Y, Miyamoto, N G
The human beta-actin promoter, including its 5' flanking region and 5' untranslated region, is ubiquitously active in mammalian cells in culture. In this report we investigated the transcriptional...
Frederickson, R M, Micheau, M R, Iwamoto, A, Miyamoto, N G
We have identified a CCAAT box element that is required for the efficient transcription of the human beta-actin gene. Both in vivo transient transfection assays in cultured HeLa cells and in vitro...
Point mutational analysis of the human c-fos serum response factor binding site.
A series of point mutants were generated in the human c-fos dyad symmetry element (DSE), found within the c-fos serum response element, to study the sequence requirements for its interaction with the...
Stimulation of transcription in vitro by binding sites for nuclear factor I.
Gronostajski, R M, Knox, J, Berry, D, Miyamoto, N G
Nuclear factor I (NFI) is a site-specific DNA binding protein required for the replication of adenovirus DNA in vitro and in vivo. We have examined the effect of natural and synthetic binding sites...
Takadera, T, Leung, S, Gernone, A, Koga, Y, Takihara, Y, Miyamoto, N G, ...
The human T-cell- or lymphocyte-specific gene, lck, encodes a tyrosine kinase and is a member of the src family. In this report we demonstrate that there are two classes of human lck transcripts...
Miyamoto, N G, Moncollin, V, Egly, J M, Chambon, P
Stimulation of in vitro transcription mediated by the upstream element of the adenovirus-2 major late promoter (Ad2MLP) involves its recognition by a specific trans-acting factor present in a HeLa...
Is actin a transcription initiation factor for RNA polymerase B?
Egly, J M, Miyamoto, N G, Moncollin, V, Chambon, P
We have previously reported that two fractions derived from HeLa cell S100 extracts, the heparin flow-through and the heparin 0.6 M KCl eluate are required in vitro for efficient and accurate...
Purification of a factor specific for the upstream element of the adenovirus-2 major late promoter.
Moncollin, V, Miyamoto, N G, Zheng, X M, Egly, J M
Stimulation of in vitro transcription by the upstream element (UE) of the adenovirus-2 major late promoter (Ad2MLP) involves a specific trans-acting factor present in a HeLa whole-cell extract. By...