N. Gotoh

Publication List Details

Period

2006 - 2006

Number

46

Co-Authors

Stimulation of phosphatidylinositol 3-kinase by fibroblast growth factor receptors is mediated by coordinated recruitment of multiple docking proteins

Ong, S. H., Hadari, Y. R., Gotoh, N., Guy, G. R., Schlessinger, J., Lax, I.

The docking protein FRS2 is a major downstream effector that links fibroblast growth factor (FGF) and nerve growth factor receptors with the Ras/mitogen-activated protein kinase signaling cascade. In...

Critical role for the docking-protein FRS2α in FGF receptor-mediated signal transduction pathways

Hadari, Y. R., Gotoh, N., Kouhara, H., Lax, I., Schlessinger, J.

The docking protein FRS2α has been implicated as a mediator of signaling via fibroblast growth factor receptors (FGFRs). We have demonstrated that targeted disruption of FRS2α gene causes severe...

Epidermal growth factor-receptor mutant lacking the autophosphorylation sites induces phosphorylation of Shc protein and Shc-Grb2/ASH association and retains mitogenic activity.

Gotoh, N, Tojo, A, Muroya, K, Hashimoto, Y, Hattori, S, Nakamura, S, ...

Epidermal growth factor (EGF) receptor (EGFR) can induce cell growth and transformation in a ligand-dependent manner. To examine whether the autophosphorylation of EGFR correlates with the capacity...

FRS2 Proteins Recruit Intracellular Signaling Pathways by Binding to Diverse Targets on Fibroblast Growth Factor and Nerve Growth Factor Receptors

Ong, S. H., Guy, G. R., Hadari, Y. R., Laks, S., Gotoh, N., Schlessinger, J., ...

The docking protein FRS2 was implicated in the transmission of extracellular signals from the fibroblast growth factor (FGF) or nerve growth factor (NGF) receptors to the Ras/mitogen-activated...

nfxC-type quinolone resistance in a clinical isolate of Pseudomonas aeruginosa.

Fukuda, H, Hosaka, M, Iyobe, S, Gotoh, N, Nishino, T, Hirai, K

Quinolone resistance gene nqr-T91 in a clinical isolate of Pseudomonas aeruginosa P1481 was cotransducible with catA1 in P. aeruginosa PAO. The nqr-T91 transductant, PKH-T91, was resistant to...

Purification of a 54-kilodalton protein (OprJ) produced in NfxB mutants of Pseudomonas aeruginosa and production of a monoclonal antibody specific to OprJ.

Hosaka, M, Gotoh, N, Nishino, T

The 54-kDa outer membrane protein (designated OprJ) of a norfloxacin-resistant nfxB mutant of Pseudomonas aeruginosa PAO1 was purified by ion-exchange high-performance liquid chromatography. Mobility...

The outer membrane protein OprM of Pseudomonas aeruginosa is encoded by oprK of the mexA-mexB-oprK multidrug resistance operon.

Gotoh, N, Tsujimoto, H, Poole, K, Yamagishi, J, Nishino, T

An outer membrane protein (OprK) overproduced in a multiply antibiotic-resistant strain of Pseudomonas aeruginosa was previously identified as the product of the third gene of a multidrug resistance...

Quantitative correlation between susceptibility and OprJ production in NfxB mutants of Pseudomonas aeruginosa.

Masuda, N, Gotoh, N, Ohya, S, Nishino, T

Various Pseudomonas aeruginosa PAO1 NfxB mutants were isolated on agar plates containing cefpirome and ofloxacin. They were classified into type A and type B, based on the degrees of changes in their...

Multidrug efflux in intrinsic resistance to trimethoprim and sulfamethoxazole in Pseudomonas aeruginosa.

Köhler, T, Kok, M, Michea-Hamzehpour, M, Plesiat, P, Gotoh, N, Nishino, T, ...

Pseudomonas aeruginosa possesses at least two multiple drug efflux systems which are defined by the outer membrane proteins OprM and OprJ. We have found that mutants overexpressing OprM were two- and...

Efficacy of trimethoprim in murine experimental infection with a thymidine kinase-deficient mutant of Escherichia coli.

Tokunaga, T, Oka, K, Takemoto, A, Ohtsubo, Y, Gotoh, N, Nishino, T

The antimicrobial activity of trimethoprim is antagonized by thymidine in in vitro susceptibility tests. The purpose of this investigation was to determine whether this antagonism also occurred...

Influence of OprM expression on multiple antibiotic resistance in Pseudomonas aeruginosa.

Wong, K K, Poole, K, Gotoh, N, Hancock, R E

MexA-MexB-OprM is an efflux system in Pseudomonas aeruginosa. OprM overproduced from the cloned gene was able to complement OprM-deficient mutants but did not alter the resistance of a wild-type P....

Role of protein F in maintaining structural integrity of the Pseudomonas aeruginosa outer membrane.

Gotoh, N, Wakebe, H, Yoshihara, E, Nakae, T, Nishino, T

To investigate the functional role of protein F of the outer membrane of Pseudomonas aeruginosa, we isolated mutants devoid of protein F, and the defective gene was transferred to a wild-type strain...

DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane.

Hasegawa, H, Kiyokawa, E, Tanaka, S, Nagashima, K, Gotoh, N, Shibuya, M, ...

CRK belongs to a family of adaptor proteins that consist mostly of SH2 and SH3 domains. Far Western blotting with CRK SH3 has demonstrated that it binds to 135- to 145-, 160-, and 180-kDa proteins....

Tyrosine phosphorylation sites at amino acids 239 and 240 of Shc are involved in epidermal growth factor-induced mitogenic signaling that is distinct from Ras/mitogen-activated protein kinase activation.

Gotoh, N, Toyoda, M, Shibuya, M

Epidermal growth factor (EGF) induces tyrosine phosphorylation of the Shc adapter protein, which plays an important role in EGF-stimulated mitogenesis. Shc stimulates Ras/mitogen-activated protein...

Virulence of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa In Vitro and In Vivo

Aoki, S., Hirakata, Y., Kondoh, A., Gotoh, N., Yanagihara, K., Miyazaki, Y., ...

We evaluated the virulence of Pseudomonas aeruginosa carrying blaIMP, a metallo-β-lactamase gene, and the efficacy of ceftazidime, imipenem-cilastatin, and ciprofloxacin in the endogenous bacteremia...

A novel pathway from phosphorylation of tyrosine residues 239/240 of Shc, contributing to suppress apoptosis by IL-3.

Gotoh, N, Tojo, A, Shibuya, M

Interleukin 3 (IL-3) not only induces DNA synthesis of haematopoietic cells but also maintains their viability by suppressing apoptosis. IL-3 stimulates tyrosine phosphorylation of the Shc adaptor...

Tyrosine phosphorylation sites on FRS2α responsible for Shp2 recruitment are critical for induction of lens and retina

Gotoh, N., Ito, M., Yamamoto, S., Yoshino, I., Song, N., Wang, Y., ...

Early development of the lens and retina depends upon reciprocal inductive interactions between the embryonic surface ectoderm and the underlying neuroepithelium of the optic vesicle. FGF signaling...

The Docking Protein FRS2α Is an Essential Component of Multiple Fibroblast Growth Factor Responses during Early Mouse Development

Gotoh, N., Manova, K., Tanaka, S., Murohashi, M., Hadari, Y., Lee, A., ...

The docking protein FRS2α is a major mediator of fibroblast growth factor (FGF) signaling. However, the physiological role of FRS2α in vivo remains unknown. In this report, we show that Frs2α-null...

Inhibition of glutathione synthesis increases the toxicity of oxidized low-density lipoprotein to human monocytes and macrophages.

Gotoh, N, Graham, A, Nikl, E, Darley-Usmar, V M

Macrophages are thought to play an important role in the pathogenesis of atherosclerosis by mediating the oxidation of low-density lipoprotein (LDL). However, it is known that these cells show...

Essential role of Shp2-binding sites on FRS2α for corticogenesis and for FGF2-dependent proliferation of neural progenitor cells

Yamamoto, S., Yoshino, I., Shimazaki, T., Murohashi, M., Hevner, R. F., Lax, I., ...

Mammalian corticogenesis occurs through a complex process that includes neurogenesis, in which neural progenitor cells proliferate, differentiate, and migrate. It has been reported recently that...

Stimulation of phosphatidylinositol 3-kinase by fibroblast growth factor receptors is mediated by coordinated recruitment of multiple docking proteins

Ong, S. H., Hadari, Y. R., Gotoh, N., Guy, G. R., Schlessinger, J., Lax, I.

The docking protein FRS2 is a major downstream effector that links fibroblast growth factor (FGF) and nerve growth factor receptors with the Ras/mitogen-activated protein kinase signaling cascade. In...

Critical role for the docking-protein FRS2α in FGF receptor-mediated signal transduction pathways

Hadari, Y. R., Gotoh, N., Kouhara, H., Lax, I., Schlessinger, J.

The docking protein FRS2α has been implicated as a mediator of signaling via fibroblast growth factor receptors (FGFRs). We have demonstrated that targeted disruption of FRS2α gene causes severe...

Epidermal growth factor-receptor mutant lacking the autophosphorylation sites induces phosphorylation of Shc protein and Shc-Grb2/ASH association and retains mitogenic activity.

Gotoh, N, Tojo, A, Muroya, K, Hashimoto, Y, Hattori, S, Nakamura, S, ...

Epidermal growth factor (EGF) receptor (EGFR) can induce cell growth and transformation in a ligand-dependent manner. To examine whether the autophosphorylation of EGFR correlates with the capacity...

FRS2 Proteins Recruit Intracellular Signaling Pathways by Binding to Diverse Targets on Fibroblast Growth Factor and Nerve Growth Factor Receptors

Ong, S. H., Guy, G. R., Hadari, Y. R., Laks, S., Gotoh, N., Schlessinger, J., ...

The docking protein FRS2 was implicated in the transmission of extracellular signals from the fibroblast growth factor (FGF) or nerve growth factor (NGF) receptors to the Ras/mitogen-activated...

nfxC-type quinolone resistance in a clinical isolate of Pseudomonas aeruginosa.

Fukuda, H, Hosaka, M, Iyobe, S, Gotoh, N, Nishino, T, Hirai, K

Quinolone resistance gene nqr-T91 in a clinical isolate of Pseudomonas aeruginosa P1481 was cotransducible with catA1 in P. aeruginosa PAO. The nqr-T91 transductant, PKH-T91, was resistant to...

Purification of a 54-kilodalton protein (OprJ) produced in NfxB mutants of Pseudomonas aeruginosa and production of a monoclonal antibody specific to OprJ.

Hosaka, M, Gotoh, N, Nishino, T

The 54-kDa outer membrane protein (designated OprJ) of a norfloxacin-resistant nfxB mutant of Pseudomonas aeruginosa PAO1 was purified by ion-exchange high-performance liquid chromatography. Mobility...

The outer membrane protein OprM of Pseudomonas aeruginosa is encoded by oprK of the mexA-mexB-oprK multidrug resistance operon.

Gotoh, N, Tsujimoto, H, Poole, K, Yamagishi, J, Nishino, T

An outer membrane protein (OprK) overproduced in a multiply antibiotic-resistant strain of Pseudomonas aeruginosa was previously identified as the product of the third gene of a multidrug resistance...

Quantitative correlation between susceptibility and OprJ production in NfxB mutants of Pseudomonas aeruginosa.

Masuda, N, Gotoh, N, Ohya, S, Nishino, T

Various Pseudomonas aeruginosa PAO1 NfxB mutants were isolated on agar plates containing cefpirome and ofloxacin. They were classified into type A and type B, based on the degrees of changes in their...

Multidrug efflux in intrinsic resistance to trimethoprim and sulfamethoxazole in Pseudomonas aeruginosa.

Köhler, T, Kok, M, Michea-Hamzehpour, M, Plesiat, P, Gotoh, N, Nishino, T, ...

Pseudomonas aeruginosa possesses at least two multiple drug efflux systems which are defined by the outer membrane proteins OprM and OprJ. We have found that mutants overexpressing OprM were two- and...

Efficacy of trimethoprim in murine experimental infection with a thymidine kinase-deficient mutant of Escherichia coli.

Tokunaga, T, Oka, K, Takemoto, A, Ohtsubo, Y, Gotoh, N, Nishino, T

The antimicrobial activity of trimethoprim is antagonized by thymidine in in vitro susceptibility tests. The purpose of this investigation was to determine whether this antagonism also occurred...

Influence of OprM expression on multiple antibiotic resistance in Pseudomonas aeruginosa.

Wong, K K, Poole, K, Gotoh, N, Hancock, R E

MexA-MexB-OprM is an efflux system in Pseudomonas aeruginosa. OprM overproduced from the cloned gene was able to complement OprM-deficient mutants but did not alter the resistance of a wild-type P....

Role of protein F in maintaining structural integrity of the Pseudomonas aeruginosa outer membrane.

Gotoh, N, Wakebe, H, Yoshihara, E, Nakae, T, Nishino, T

To investigate the functional role of protein F of the outer membrane of Pseudomonas aeruginosa, we isolated mutants devoid of protein F, and the defective gene was transferred to a wild-type strain...

DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane.

Hasegawa, H, Kiyokawa, E, Tanaka, S, Nagashima, K, Gotoh, N, Shibuya, M, ...

CRK belongs to a family of adaptor proteins that consist mostly of SH2 and SH3 domains. Far Western blotting with CRK SH3 has demonstrated that it binds to 135- to 145-, 160-, and 180-kDa proteins....

Tyrosine phosphorylation sites at amino acids 239 and 240 of Shc are involved in epidermal growth factor-induced mitogenic signaling that is distinct from Ras/mitogen-activated protein kinase activation.

Gotoh, N, Toyoda, M, Shibuya, M

Epidermal growth factor (EGF) induces tyrosine phosphorylation of the Shc adapter protein, which plays an important role in EGF-stimulated mitogenesis. Shc stimulates Ras/mitogen-activated protein...

Virulence of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa In Vitro and In Vivo

Aoki, S., Hirakata, Y., Kondoh, A., Gotoh, N., Yanagihara, K., Miyazaki, Y., ...

We evaluated the virulence of Pseudomonas aeruginosa carrying blaIMP, a metallo-β-lactamase gene, and the efficacy of ceftazidime, imipenem-cilastatin, and ciprofloxacin in the endogenous bacteremia...

A novel pathway from phosphorylation of tyrosine residues 239/240 of Shc, contributing to suppress apoptosis by IL-3.

Gotoh, N, Tojo, A, Shibuya, M

Interleukin 3 (IL-3) not only induces DNA synthesis of haematopoietic cells but also maintains their viability by suppressing apoptosis. IL-3 stimulates tyrosine phosphorylation of the Shc adaptor...

Tyrosine phosphorylation sites on FRS2α responsible for Shp2 recruitment are critical for induction of lens and retina

Gotoh, N., Ito, M., Yamamoto, S., Yoshino, I., Song, N., Wang, Y., ...

Early development of the lens and retina depends upon reciprocal inductive interactions between the embryonic surface ectoderm and the underlying neuroepithelium of the optic vesicle. FGF signaling...

The Docking Protein FRS2α Is an Essential Component of Multiple Fibroblast Growth Factor Responses during Early Mouse Development

Gotoh, N., Manova, K., Tanaka, S., Murohashi, M., Hadari, Y., Lee, A., ...

The docking protein FRS2α is a major mediator of fibroblast growth factor (FGF) signaling. However, the physiological role of FRS2α in vivo remains unknown. In this report, we show that Frs2α-null...

Inhibition of glutathione synthesis increases the toxicity of oxidized low-density lipoprotein to human monocytes and macrophages.

Gotoh, N, Graham, A, Nikl, E, Darley-Usmar, V M

Macrophages are thought to play an important role in the pathogenesis of atherosclerosis by mediating the oxidation of low-density lipoprotein (LDL). However, it is known that these cells show...

Essential role of Shp2-binding sites on FRS2α for corticogenesis and for FGF2-dependent proliferation of neural progenitor cells

Yamamoto, S., Yoshino, I., Shimazaki, T., Murohashi, M., Hevner, R. F., Lax, I., ...

Mammalian corticogenesis occurs through a complex process that includes neurogenesis, in which neural progenitor cells proliferate, differentiate, and migrate. It has been reported recently that...