Inelastic X-Ray Scattering Study of Exciton Properties in an Organic Molecular crystal (2006)
Yang, K., Chen, L. P., Cai, Y. Q., Hiraoka, N., Li, S., Zhao, J. F., ...
Excitons in a complex organic molecular crystal were studied by inelastic x-ray scattering (IXS) for the first time. The dynamic dielectric response function is measured over a large momentum...
Electronic structure of methane hydrate studied by compton scattering (2006)
C. Sternemann, DESY Forschung Mit Photonen, S. Huotari, M. Hakala, M. Paulus, M. Volmer, ...
Journal article
Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A
Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and...
AccIII, a new restriction endonuclease from Acinetobacter calcoaceticus.
Kita, K, Hiraoka, N, Oshima, A, Kadonishi, S, Obayashi, A
A new site-specific restriction endonuclease, AccIII, was isolated from Acinetobacter calcoaceticus. AccIII recognizes T/CCGGA and cleaves at the position shown by the arrow. AccIII activity was...
Interaction of the restriction endonuclease ScaI with its substrates.
Kita, K, Hiraoka, N, Kimizuka, F, Obayashi, A, Kojima, H, Takahashi, H, ...
The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid...
Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A
In the presence of high concentrations of the nonspecific polymer polyethylene glycol (PEG), intermolecular cohesive-end ligation with the DNA ligase from Escherichia coli was stimulated by high salt...
Cloning, nucleotide sequence, and expression of the HincII restriction-modification system.
Ito, H, Sadaoka, A, Kotani, H, Hiraoka, N, Nakamura, T
Two genes, coding for the HincII from Haemophilus influenzae Rc restriction-modification system, were cloned and expressed in Escherichia coli RR1. Their DNA sequences were determined. The HincII...
Overproduction and crystallization of FokI restriction endonuclease.
Kita, K, Kotani, H, Hiraoka, N, Nakamura, T, Yonaha, K
To overproduce FokI endonuclease (R.FokI) in an Escherichia coli system, the coding region of R.FokI predicted from the nucleotide sequence was generated from the FokI operon and joined to the tac...
A new restriction endonuclease from Spirulina platensis.
Kawamura, M, Sakakibara, M, Watanabe, T, Kita, K, Hiraoka, N, Obayashi, A, ...
Three restriction endonucleases, Sp1I, Sp1II and Sp1III have been purified partially from Spirulina platensis subspecies siamese and named. Sp1I cleaves bacteriophage lambda DNA at one site, phi X...
Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase.
Kotani, H, Ishizaki, Y, Hiraoka, N, Obayashi, A
The coding region of the gene for bacteriophage SP6 RNA polymerase was cloned into pBR322, and its entire nucleotide sequence was deduced. The predicted amino acid sequence for the polymerase...
Site-specific dissection of E. coli chromosome by lambda terminase.
Kotani, H, Kawamura, A, Takahashi, A, Nakatsuji, M, Hiraoka, N, Nakajima, K, ...
We have succeeded the targeted cleavage of chromosomes by lambda terminase that introduces double-strand cleavages in DNA recognizing the lambda cos sequence. When chromosomal DNAs of various...
Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A
Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and...
Site-specific dissection of E. coli chromosome by lambda terminase.
Kotani, H, Kawamura, A, Takahashi, A, Nakatsuji, M, Hiraoka, N, Nakajima, K, ...
We have succeeded the targeted cleavage of chromosomes by lambda terminase that introduces double-strand cleavages in DNA recognizing the lambda cos sequence. When chromosomal DNAs of various...
AccIII, a new restriction endonuclease from Acinetobacter calcoaceticus.
Kita, K, Hiraoka, N, Oshima, A, Kadonishi, S, Obayashi, A
A new site-specific restriction endonuclease, AccIII, was isolated from Acinetobacter calcoaceticus. AccIII recognizes T/CCGGA and cleaves at the position shown by the arrow. AccIII activity was...
Interaction of the restriction endonuclease ScaI with its substrates.
Kita, K, Hiraoka, N, Kimizuka, F, Obayashi, A, Kojima, H, Takahashi, H, ...
The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid...
Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A
In the presence of high concentrations of the nonspecific polymer polyethylene glycol (PEG), intermolecular cohesive-end ligation with the DNA ligase from Escherichia coli was stimulated by high salt...
Cloning, nucleotide sequence, and expression of the HincII restriction-modification system.
Ito, H, Sadaoka, A, Kotani, H, Hiraoka, N, Nakamura, T
Two genes, coding for the HincII from Haemophilus influenzae Rc restriction-modification system, were cloned and expressed in Escherichia coli RR1. Their DNA sequences were determined. The HincII...
Overproduction and crystallization of FokI restriction endonuclease.
Kita, K, Kotani, H, Hiraoka, N, Nakamura, T, Yonaha, K
To overproduce FokI endonuclease (R.FokI) in an Escherichia coli system, the coding region of R.FokI predicted from the nucleotide sequence was generated from the FokI operon and joined to the tac...
A new restriction endonuclease from Spirulina platensis.
Kawamura, M, Sakakibara, M, Watanabe, T, Kita, K, Hiraoka, N, Obayashi, A, ...
Three restriction endonucleases, Sp1I, Sp1II and Sp1III have been purified partially from Spirulina platensis subspecies siamese and named. Sp1I cleaves bacteriophage lambda DNA at one site, phi X...
Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase.
Kotani, H, Ishizaki, Y, Hiraoka, N, Obayashi, A
The coding region of the gene for bacteriophage SP6 RNA polymerase was cloned into pBR322, and its entire nucleotide sequence was deduced. The predicted amino acid sequence for the polymerase...
Contraction of rat thoracic aorta strips by endothelin-1 in the absence of extracellular Ca2+.
Itoh, H., Higuchi, H., Hiraoka, N., Ito, M., Konishi, T., Nakano, T., ...
1. Endothelin-1 (ET-1) caused a concentration-dependent contraction of helical strips from rat thoracic aorta in the absence of extracellular Ca2+. The Ca(2+)-depleted muscle strips, prepared by...