Ectopic lymphokine gene expression in human peripheral blood lymphocytes in vivo.
Chambers, C A, Kang, J, Hozumi, N
An animal model to study the effects of ectopic expression of cytokines involved in cell growth and differentiation has been established. Retrovirus vectors containing the human interleukin 6 cDNA...
To determine the feasibility of retrovirus-mediated gene transfer into stem cells for studying T-cell development, we constructed a high-titer retrovirus vector containing the neomycin...
Bast, D J, Sandhu, J, Hozumi, N, Barber, B, Brunton, J
Structurally conserved verotoxin 1 (VT1) mutant derivatives, showing reduced receptor binding and cytotoxicity, may serve as natural toxoids to protect against VT-mediated disease. In this study, the...
Tisch, R, Roifman, C M, Hozumi, N
Crosslinked IgM molecules expressed on the surface of immature B cells mediate responses that inhibit further development, in contrast to the activational and proliferative events that follow...
Tisch, R, Watanabe, M, Letarte, M, Hozumi, N
Monoclonal B-cell lines expressing antigen-specific surface IgM or IgD were established by transferring the genes encoding immunoglobulin heavy (mu or delta) and light (kappa) chains specific for the...
Nucleotide sequence of a chromosomal rearranged lambda 2 immunoglobulin gene of mouse.
Wu, G E, Govindji, N, Hozumi, N, Murialdo, H
The rearranged lambda 2 gene of the mouse plasmacytoma cell line MOPC315 has been cloned and sequenced. A comparison of its sequence with the sequence of the unrearranged (germ-line) V, J and C gene...
Watanabe, M, Wegmann, D R, Ochi, A, Hozumi, N
The rearranged genes encoding immunoglobulin heavy (mu) and light (kappa) chains specific for the hapten 2,4,6-trinitrophenyl (Tnp) were introduced into a B-lymphoma line that bears surface IgG with...
Nucleotide sequences of immunoglobulin mu heavy chain deletion mutants.
Baczynsky, W O, Sugii, S, Murialdo, H, Pennell, N, Filkin, C, Hozumi, N, ...
Mutants of an IgM producing hybridoma cell line were isolated which produce mu heavy chain fragments. Two such mutants were found to have internal deletions in the mu gene and the nucleotide sequence...
Characterization of a mouse DNA clone containing an immunoglobulin variable region gene.
Hozumi, N, Brack, C, Pirrotta, V, Lenhard-Schuller, R, Tonegawa, S
A 4.8 kilobase mouse embryo DNA fragment was inserted into a phage lambda genome and was subsequently characterized by electron microscopy, restriction enzyme mapping and partial DNA sequencing. This...
Mutant immunoglobulin genes have repetitive DNA elements inserted into their intervening sequences.
Hawley, R G, Shulman, M J, Murialdo, H, Gibson, D M, Hozumi, N
The kappa light chain genes from two mutant hybridoma cell lines defective in kappa light chain synthesis were isolated and compared to the wild-type kappa light chain gene. In each case, the mutant...
RNA splicing mutation in an aberrantly rearranged immunoglobulin lambda I gene.
Hozumi, N, Wu, G E, Murialdo, H, Roberts, L, Vetter, D, Fife, W L, ...
The mouse cell line MOPC 315 is an IgA (lambda II)-producing myeloma. We have studied a derivative of MOPC 315 that secretes normal lambda II chains but no heavy chain. This derivative, MOPC 315-26,...
Parameters that govern the regulation of immunoglobulin delta heavy-chain gene expression.
The mu and delta immunoglobulin heavy-chain genes comprise a complex transcriptional unit in which a single mRNA precursor gives rise to mu- and delta-specific transcripts. During the immature B-cell...
Tanaka, K, Gorelik, E, Watanabe, M, Hozumi, N, Jay, G
Transfection of a functional major histocompatibility complex class I gene into certain tumor cells, induced by oncogenic viruses or chemical carcinogens, can effectively abrogate their tumorigenic...
Hawley, R G, Shulman, M J, Hozumi, N
Each of two severely defective mouse kappa-chain genes has acquired a different intracisternal A particle (IAP) element within one of its introns. One IAP element generated 6-base-pair direct repeats...
Shulman, M J, Pennell, N, Collins, C, Hozumi, N
We have isolated and analyzed the DNA encoding the mu heavy chain constant region of a mutant IgM that is defective in initiating complement-dependent cytolysis. By assaying the expression of...
Insertion of a bacterial gene into the mouse germ line using an infectious retrovirus vector.
Huszar, D, Balling, R, Kothary, R, Magli, M C, Hozumi, N, Rossant, J, ...
Using a Moloney leukemia virus vector containing the bacterial neo gene, we demonstrate that retrovirus vectors can be used to introduce genes into the mouse germ line. Infection of preimplantation...
Intracisternal A-particle genes as movable elements in the mouse genome.
Kuff, E L, Feenstra, A, Lueders, K, Smith, L, Hawley, R, Hozumi, N, ...
We analyzed two functionally defective mouse kappa light chain gene variants previously shown to contain novel insertions of repetitive DNA in their intervening sequences [Hawley, R. G., Shulman, M....
Ochi, A, Hawley, R G, Hawley, T, Shulman, M J, Traunecker, A, Köhler, G, ...
The rearranged immunoglobulin heavy (mu) and light (kappa) chain genes cloned from the Sp6 hybridoma cell line producing immunoglobulin M specific for the hapten 2,4, 6-trinitrophenyl were inserted...
Immunoglobulin mu-chain gene rearrangement in a patient with T cell acute lymphoblastic leukemia.
Ha, K, Minden, M, Hozumi, N, Gelfand, E W
A 6-yr-old girl with T cell acute lymphoblastic leukemia (ALL) is described. She had a mediastinal mass and her leukemic cells expressed T cell-associated antigens (Leu 1+, OKT3+, OKT9+, and OKT10+)....
Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.
A high-molecular-weight DNA from Balb/c mouse early embryo or from MOPC 321 plasmacytoma (a k-chain producer) was digested to completion with Bacillus amyloliquefaciens strain H restriction enzyme...
Cloning of an immunoglobulin variable region gene from mouse embryo.
Tonegawa, S, Brack, C, Hozumi, N, Schuller, R
A 4.8-kilobase DNA fragment carrying an immunoglobulin gene coding for a mouse lambda chain variable region (Vlambda gene) was enriched about 350-fold from a total endonuclease EcoRI digest of...
Chambers, C A, Kang, J, Pawling, J, Huber, B, Hozumi, N, Nagy, A
Direct genetic manipulation of hematopoietic cells is limited by the lack of an established hematopoietic stem cell line. It has been demonstrated that embryonic stem (ES) celltetraploid embryos are...
Ectopic lymphokine gene expression in human peripheral blood lymphocytes in vivo.
Chambers, C A, Kang, J, Hozumi, N
An animal model to study the effects of ectopic expression of cytokines involved in cell growth and differentiation has been established. Retrovirus vectors containing the human interleukin 6 cDNA...
To determine the feasibility of retrovirus-mediated gene transfer into stem cells for studying T-cell development, we constructed a high-titer retrovirus vector containing the neomycin...
Bast, D J, Sandhu, J, Hozumi, N, Barber, B, Brunton, J
Structurally conserved verotoxin 1 (VT1) mutant derivatives, showing reduced receptor binding and cytotoxicity, may serve as natural toxoids to protect against VT-mediated disease. In this study, the...
Tisch, R, Roifman, C M, Hozumi, N
Crosslinked IgM molecules expressed on the surface of immature B cells mediate responses that inhibit further development, in contrast to the activational and proliferative events that follow...
Tisch, R, Watanabe, M, Letarte, M, Hozumi, N
Monoclonal B-cell lines expressing antigen-specific surface IgM or IgD were established by transferring the genes encoding immunoglobulin heavy (mu or delta) and light (kappa) chains specific for the...
Nucleotide sequence of a chromosomal rearranged lambda 2 immunoglobulin gene of mouse.
Wu, G E, Govindji, N, Hozumi, N, Murialdo, H
The rearranged lambda 2 gene of the mouse plasmacytoma cell line MOPC315 has been cloned and sequenced. A comparison of its sequence with the sequence of the unrearranged (germ-line) V, J and C gene...
Watanabe, M, Wegmann, D R, Ochi, A, Hozumi, N
The rearranged genes encoding immunoglobulin heavy (mu) and light (kappa) chains specific for the hapten 2,4,6-trinitrophenyl (Tnp) were introduced into a B-lymphoma line that bears surface IgG with...
Nucleotide sequences of immunoglobulin mu heavy chain deletion mutants.
Baczynsky, W O, Sugii, S, Murialdo, H, Pennell, N, Filkin, C, Hozumi, N, ...
Mutants of an IgM producing hybridoma cell line were isolated which produce mu heavy chain fragments. Two such mutants were found to have internal deletions in the mu gene and the nucleotide sequence...
Characterization of a mouse DNA clone containing an immunoglobulin variable region gene.
Hozumi, N, Brack, C, Pirrotta, V, Lenhard-Schuller, R, Tonegawa, S
A 4.8 kilobase mouse embryo DNA fragment was inserted into a phage lambda genome and was subsequently characterized by electron microscopy, restriction enzyme mapping and partial DNA sequencing. This...
Mutant immunoglobulin genes have repetitive DNA elements inserted into their intervening sequences.
Hawley, R G, Shulman, M J, Murialdo, H, Gibson, D M, Hozumi, N
The kappa light chain genes from two mutant hybridoma cell lines defective in kappa light chain synthesis were isolated and compared to the wild-type kappa light chain gene. In each case, the mutant...
RNA splicing mutation in an aberrantly rearranged immunoglobulin lambda I gene.
Hozumi, N, Wu, G E, Murialdo, H, Roberts, L, Vetter, D, Fife, W L, ...
The mouse cell line MOPC 315 is an IgA (lambda II)-producing myeloma. We have studied a derivative of MOPC 315 that secretes normal lambda II chains but no heavy chain. This derivative, MOPC 315-26,...
Parameters that govern the regulation of immunoglobulin delta heavy-chain gene expression.
The mu and delta immunoglobulin heavy-chain genes comprise a complex transcriptional unit in which a single mRNA precursor gives rise to mu- and delta-specific transcripts. During the immature B-cell...
Tanaka, K, Gorelik, E, Watanabe, M, Hozumi, N, Jay, G
Transfection of a functional major histocompatibility complex class I gene into certain tumor cells, induced by oncogenic viruses or chemical carcinogens, can effectively abrogate their tumorigenic...
Hawley, R G, Shulman, M J, Hozumi, N
Each of two severely defective mouse kappa-chain genes has acquired a different intracisternal A particle (IAP) element within one of its introns. One IAP element generated 6-base-pair direct repeats...
Shulman, M J, Pennell, N, Collins, C, Hozumi, N
We have isolated and analyzed the DNA encoding the mu heavy chain constant region of a mutant IgM that is defective in initiating complement-dependent cytolysis. By assaying the expression of...
Insertion of a bacterial gene into the mouse germ line using an infectious retrovirus vector.
Huszar, D, Balling, R, Kothary, R, Magli, M C, Hozumi, N, Rossant, J, ...
Using a Moloney leukemia virus vector containing the bacterial neo gene, we demonstrate that retrovirus vectors can be used to introduce genes into the mouse germ line. Infection of preimplantation...
Intracisternal A-particle genes as movable elements in the mouse genome.
Kuff, E L, Feenstra, A, Lueders, K, Smith, L, Hawley, R, Hozumi, N, ...
We analyzed two functionally defective mouse kappa light chain gene variants previously shown to contain novel insertions of repetitive DNA in their intervening sequences [Hawley, R. G., Shulman, M....
Ochi, A, Hawley, R G, Hawley, T, Shulman, M J, Traunecker, A, Köhler, G, ...
The rearranged immunoglobulin heavy (mu) and light (kappa) chain genes cloned from the Sp6 hybridoma cell line producing immunoglobulin M specific for the hapten 2,4, 6-trinitrophenyl were inserted...
Immunoglobulin mu-chain gene rearrangement in a patient with T cell acute lymphoblastic leukemia.
Ha, K, Minden, M, Hozumi, N, Gelfand, E W
A 6-yr-old girl with T cell acute lymphoblastic leukemia (ALL) is described. She had a mediastinal mass and her leukemic cells expressed T cell-associated antigens (Leu 1+, OKT3+, OKT9+, and OKT10+)....
Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.
A high-molecular-weight DNA from Balb/c mouse early embryo or from MOPC 321 plasmacytoma (a k-chain producer) was digested to completion with Bacillus amyloliquefaciens strain H restriction enzyme...
Cloning of an immunoglobulin variable region gene from mouse embryo.
Tonegawa, S, Brack, C, Hozumi, N, Schuller, R
A 4.8-kilobase DNA fragment carrying an immunoglobulin gene coding for a mouse lambda chain variable region (Vlambda gene) was enriched about 350-fold from a total endonuclease EcoRI digest of...
Chambers, C A, Kang, J, Pawling, J, Huber, B, Hozumi, N, Nagy, A
Direct genetic manipulation of hematopoietic cells is limited by the lack of an established hematopoietic stem cell line. It has been demonstrated that embryonic stem (ES) celltetraploid embryos are...
Murine lymphocytes with natural killer activity express CTL-derived serine protease genes.
Anderson, S K, Hong, K K, Hoskin, D W, Abramow-Newerly, W, Fulop, G M, Phillips, R A, ...
Serine protease genes (C11, B10 and HF) derived from activated cytolytic T lymphocytes have been shown to be important in CTL-mediated cytotoxicity. In this study, we examined the expression of these...
Effect of Mycoplasma arthritidis superantigen on enzymatically induced arthritis in mice.
Sustackova, H, Chambers, C A, Hozumi, N, Kandel, R A, Quinn, P A, Cruz, T F
The objective of this study was to examine the effect of the stimulation of the immune system with Mycoplasma arthritidis superantigen (MAS) on joint inflammation and cartilage destruction. MAS was...
CD4+ cytolytic T cell clones that recognize polymorphism of HLA-DR beta 3 chains.
Noguchi, M, Hozumi, N, Nisbet-Brown, E
We have investigated HLA-DR beta 3-associated functional polymorphism using selected Epstein-Barr virus (EBV) specific human T cell clones and EBV-transformed B cell (EBV-B) lines. To study the...
Functional heterogeneity of human T cell clones from atopic and non-atopic donors.
Chambers, C A, Zimmerman, B, Hozumi, N
Several distinct T helper (TH) subsets have been identified, based on the cytokines secreted. Recently, it has been demonstrated that these subsets can regulate the isotype of humoral responses. To...
Nguyen, H, Hay, J, Mazzulli, T, Gallinger, S, Sandhu, J, Teng, Y-T A, ...
RSV is one of the major causes of pneumonia and bronchiolitis in infants and young children and is associated with high mortality. RSV neutralizing human antibody (hu-Ab) is known to mediate...