N. L. Daly

Publication List Details

Period

1999 - 2009

Number

114

Co-Authors

Inhibition of Neuronal Nicotinic Acetylcholine Receptor Subtypes by alpha-Conotoxin GID and Analogues (2009)

Millard, E. L., Nevin, S. T., Loughnan, M. L., Nicke, A., Clark, R. J., Alewood, P. F., ...

alpha-Conotoxins are small disulfide-rich peptides from the venom of the Conus species that target the nicotinic acetylcholine receptor (nAChR). They are valuable pharmacological tools and also have...

Molecular engineering of conotoxins: The importance of loop size to alpha-conotoxin structure and function (2008)

Jin, A. H., Daly, N. L., Nevin, S. T., Wang, C. I. A., Dutertre, S., Lewis, R. J., ...

alpha-Conotoxins are competitive antagonists of nicotinic acetylcholine receptors (nAChRs). The majority of currently characterized alpha-conotoxins have a 4/7 loop size, and the major features of...

The three-dimensional structure of the analgesic alpha-conotoxin, RgIA (2008)

Clark, R. J., Daly, N. L., Halai, R., Nevin, S. T., Adams, D. J., Craik, D. J.

The alpha-conotoxin RgIA is a selective antagonist of the alpha 9 alpha 10 nicotinic acetylcholine receptor and has been shown to be a potent analgesic and reduces nerve injury associated...

The structure of a two-disulfide intermediate assists in elucidating the oxidative folding pathway of a cyclic cystine knot protein (2008)

Cemazar, M., Joshi, A., Daly, N. L., Mark, A. E., Craik, D. J.

We have determined the three-dimensional structure of a two-disulfide intermediate (Cys8-Cys20, Cys14-Cys26) on the oxidative folding pathway of the cyclotide MCoTI-II. Cyclotides have a range of...

NMR as a tool for elucidating the structures of circular and knotted proteins (2007)

Craik, D. J, Daly, N. L

Cyclotides are a recently discovered family of mini-proteins that have a head-to-tail cyclised backbone stabilized by a knotted arrangement of three disulfide bonds. They have a wide range of...

Potential therapeutic applications of the cyclotides and related cystine knot mini-proteins (2007)

Craik, D. J, Clark, R. J, Daly, N. L

Cyclotides are naturally occurring mini-proteins that have a cyclic peptide backbone and a knotted arrangement of three disulfide bonds. They are remarkably stable and have a diverse range of...

The chemistry and biology of cyclotides (2007)

Craik, D. J., Cemazar, M., Daly, N. L.

Cyclotides are mini-proteins with a macrocyclic peptide backbone and cystine-knot arrangement of disulfide bonds that makes them exceptionally stable to chemical, thermal or enzymatic degradation....

The cyclic cystine knot miniprotein MCoTI-II is internalized into cells by macropinocytosis (2007)

Greenwood, K. P., Daly, N. L., Brown, D. L., Stow, J. L., Craik, D. J.

The cyclotides are macrocyclic knotted proteins characterized by a compact topology and exceptional stability. Accordingly it has been hypothesized that they may be useful as protein engineering...

The cyclotide fingerprint in Oldenlandia affinis: Elucidation of chemically modified, linear and novel macrocyclic peptides (2007)

Plan, M. R. R, Goransson, U, Clark, R. J, Daly, N. L, Colgrave, M. L, Craik, D.J

The complete suite of cyclotides present in Oldenlandia affinis (Rubiaceae), the plant that was originally found to contain this unique family of circular proteins, has been characterised. This study...

The cyclic cystine knot miniprotein MCoTI-II is internalized into cells by macropinocytosis (2007)

Greenwood, K. P., Daly, N. L., Brown, D. L., Stow, J. L., Craik, D. J.

The cyclotides are macrocyclic knotted proteins characterized by a compact topology and exceptional stability. Accordingly it has been hypothesized that they may be useful as protein engineering...

NMR as a tool for elucidating the structures of circular and knotted proteins (2007)

Craik, D. J, Daly, N. L

Cyclotides are a recently discovered family of mini-proteins that have a head-to-tail cyclised backbone stabilized by a knotted arrangement of three disulfide bonds. They have a wide range of...

Potential therapeutic applications of the cyclotides and related cystine knot mini-proteins (2007)

Craik, D. J, Clark, R. J, Daly, N. L

Cyclotides are naturally occurring mini-proteins that have a cyclic peptide backbone and a knotted arrangement of three disulfide bonds. They are remarkably stable and have a diverse range of...

The cyclotide fingerprint in Oldenlandia affinis: Elucidation of chemically modified, linear and novel macrocyclic peptides (2007)

Plan, M. R. R, Goransson, U, Clark, R. J, Daly, N. L, Colgrave, M. L, Craik, D.J

The complete suite of cyclotides present in Oldenlandia affinis (Rubiaceae), the plant that was originally found to contain this unique family of circular proteins, has been characterised. This study...

The chemistry and biology of cyclotides (2007)

Craik, D. J., Cemazar, M., Daly, N. L.

Cyclotides are mini-proteins with a macrocyclic peptide backbone and cystine-knot arrangement of disulfide bonds that makes them exceptionally stable to chemical, thermal or enzymatic degradation....

Cyclic MrIA: A stable and potent cyclic conotoxin with a novel topological fold that targets the norepinephrine transporter (2006)

Lovelace, E. S., Armishaw, C. J., Colgrave, M. L., Wahlstrom, M. E., Alewood, P. F., Daly, N. L., ...

Conotoxins, disulfide-rich peptides from the venom of cone snails, have created much excitement over recent years due to their potency and specificity for ion channels and their therapeutic...

A novel conotoxin inhibitor of Kv1.6 channel and nAChR subtypes defines a new superfamily of conotoxins (2006)

Imperial, J. S., Bansal, P. S., Daly, N. L., Craik, D. ., Sporning, A., ...

Using assay-directed fractionation of the venom from the vermivorous cone snail Conus planorbis, we isolated a new conotoxin, designated p114a, with potent activity at both nicotinic acetylcholine...

Chemical synthesis and biosynthesis of the cyclotide family of circular proteins (2006)

Gunasekera, S., Daly, N. L., Anderson, M. A., Craik, D. J.

Cyclotides are a recently discovered class of proteins that have a characteristic head-to-tail cyclized backbone stabilized by a knotted arrangement of three disulfide bonds. They are exceptionally...

Chemical synthesis and structure elucidation of bovine kappa-casein (1-44) (2006)

Bansal, P. S., Grieve, P. A., Marschke, R. J., Daly, N. L., McGhie, E., Craik, D. J., ...

The caseins (alpha(s1), alpha(s2), beta, and kappa) are phosphoproteins present in bovine milk that have been studied for over a century and whose structures remain obscure. Here we describe the...

Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif (2006)

Daly, N. L., Clark, R. J., Plan, M. R., Craik, D. J.

The cyclotides are a family of circular proteins with a range of biological activities and potential pharmaceutical and agricultural applications. The biosynthetic mechanism of cyclization is unknown...

Knots in rings - The circular knotted protein momordica cochinchinensis trypsin inhibitor-II folds via a stable two-disulfide intermediate (2006)

Cemazar, M., Daly, N. L., Haggblad, S., Lo, K. P., Yulyaningsih, E., Craik, D. J.

The aim of this work was to elucidate the oxidative folding mechanism of the macrocyclic cystine knot protein MCoTI-II. We aimed to investigate how the six-cysteine residues distributed on the...

NMR of conotoxins: structural features and an analysis of chemical shifts of post-translationally modified amino acids (2006)

Marx, U. C., Daly, N. L., Craik, D. J.

Conotoxins are small conformationally constrained peptides found in the venom of marine snails of the genus Conus. They are usually cysteine rich and frequently contain a high degree of...

Solution structure and characterization of the LGR8 receptor binding surface of insulin-like peptide 3 (2006)

Rosengren, K. J., Zhang, S., Lin, F., Daly, N. L., Scott, D. J., Hughes, R. A., ...

Insulin-like peptide 3 (INSL3), a member of the relaxin peptide family, is produced in testicular Leydig cells and ovarian thecal cells. Gene knock-out experiments have identified a key biological...

Solution structure and novel insights into the determinants of the receptor specificity of human relaxin-3 (2006)

Rosengren, K. J., Lin, F., Bathgate, R. A. D., Tregear, G. W., Daly, N. L., Wade, J. D., ...

Relaxin- 3 is the most recently discovered member of the relaxin family of peptide hormones. In contrast to relaxin- 1 and - 2, whose main functions are associated with pregnancy, relaxin- 3 is...

Structural and functional characterization of the conserved salt bridge in mammalian Paneth cell alpha-defensins - Solution structures of mouse cryptdin-4 AND (E15D)-cryptdin-4 (2006)

Rosengren, K. J., Daly, N. L., Fornander, M., Jonsson, M. H., Shirafuji, Y., Qu, X. Q., ...

alpha-Defensins are mediators of mammalian innate immunity, and knowledge of their structure-function relationships is essential for understanding their mechanisms of action. We report here the NMR...

Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design (2006)

Clark, R. J., Daly, N. L., Craik, D. J.

The cyclotide family of plant proteins is of interest because of their unique topology, which combines a head-to-tail cyclic backbone with an embedded cystine knot, and because their-remarkable...

The absolute structural requirement for a proline in the P3 '-position of Bowman-Birk protease inhibitors is surmounted in the minimized SFTI-1 scaffold (2006)

Daly, N. L., Chen, Y. K., Foley, F. M., Bansal, P. S., Bharathi, R., Clark, R. J., ...

SFTI-1 is a small cyclic peptide from sunflower seeds that is one of the most potent trypsin inhibitors of any naturally occurring peptide and is related to the Bowman-Birk family of inhibitors...

The cyclotide family of circular miniproteins: Nature's combinatorial peptide template (2006)

Craik, D. J., Cemazar, M., Wang, C. K. L., Daly, N. L.

The cyclotides are a recently discovered family of miniproteins that contain a head-to-tail cyclized backbone and a knotted arrangement of disulfide bonds. They are approximately 30 amino acids in...

The cyclotides and related macrocyclic peptides as scaffolds in drug design (2006)

Craik, D. J., Cemazar, M., Daly, N. L.

The applicability of linear peptides as drugs is potentially limited by their susceptibility to proteolytic cleavage and poor bioavailability. Cyclotides are macrocyclic cystine-knotted mini-proteins...

theta-defensins prevent HIV-1 Env-mediated fusion by binding gp41 and blocking 6-helix bundle formation (2006)

Gallo, S. A., Wang, W., Rawat, S. S., Jung, G., Waring, A. J., Cole, A. M., ...

Retrocyclin-1, a 0-defensin, protects target cells from human immunodeficiency virus, type 1 (HIV-1) by preventing viral entry. To delineate its mechanism, we conducted fusion assays between...

A novel conotoxin inhibitor of Kv1.6 channel and nAChR subtypes defines a new superfamily of conotoxins (2006)

Imperial, J. S., Bansal, P. S., Daly, N. L., Craik, D. ., Sporning, A., ...

Using assay-directed fractionation of the venom from the vermivorous cone snail Conus planorbis, we isolated a new conotoxin, designated p114a, with potent activity at both nicotinic acetylcholine...

Chemical synthesis and biosynthesis of the cyclotide family of circular proteins (2006)

Gunasekera, S., Daly, N. L., Anderson, M. A., Craik, D. J.

Cyclotides are a recently discovered class of proteins that have a characteristic head-to-tail cyclized backbone stabilized by a knotted arrangement of three disulfide bonds. They are exceptionally...

Chemical synthesis and structure elucidation of bovine kappa-casein (1-44) (2006)

Bansal, P. S., Grieve, P. A., Marschke, R. J., Daly, N. L., McGhie, E., Craik, D. J., ...

The caseins (alpha(s1), alpha(s2), beta, and kappa) are phosphoproteins present in bovine milk that have been studied for over a century and whose structures remain obscure. Here we describe the...

Cyclic MrIA: A stable and potent cyclic conotoxin with a novel topological fold that targets the norepinephrine transporter (2006)

Lovelace, E. S., Armishaw, C. J., Colgrave, M. L., Wahlstrom, M. E., Alewood, P. F., Daly, N. L., ...

Conotoxins, disulfide-rich peptides from the venom of cone snails, have created much excitement over recent years due to their potency and specificity for ion channels and their therapeutic...

Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif (2006)

Daly, N. L., Clark, R. J., Plan, M. R., Craik, D. J.

The cyclotides are a family of circular proteins with a range of biological activities and potential pharmaceutical and agricultural applications. The biosynthetic mechanism of cyclization is unknown...

Knots in rings - The circular knotted protein momordica cochinchinensis trypsin inhibitor-II folds via a stable two-disulfide intermediate (2006)

Cemazar, M., Daly, N. L., Haggblad, S., Lo, K. P., Yulyaningsih, E., Craik, D. J.

The aim of this work was to elucidate the oxidative folding mechanism of the macrocyclic cystine knot protein MCoTI-II. We aimed to investigate how the six-cysteine residues distributed on the...

NMR of conotoxins: structural features and an analysis of chemical shifts of post-translationally modified amino acids (2006)

Marx, U. C., Daly, N. L., Craik, D. J.

Conotoxins are small conformationally constrained peptides found in the venom of marine snails of the genus Conus. They are usually cysteine rich and frequently contain a high degree of...

Solution structure and characterization of the LGR8 receptor binding surface of insulin-like peptide 3 (2006)

Rosengren, K. J., Zhang, S., Lin, F., Daly, N. L., Scott, D. J., Hughes, R. A., ...

Insulin-like peptide 3 (INSL3), a member of the relaxin peptide family, is produced in testicular Leydig cells and ovarian thecal cells. Gene knock-out experiments have identified a key biological...

Solution structure and novel insights into the determinants of the receptor specificity of human relaxin-3 (2006)

Rosengren, K. J., Lin, F., Bathgate, R. A. D., Tregear, G. W., Daly, N. L., Wade, J. D., ...

Relaxin- 3 is the most recently discovered member of the relaxin family of peptide hormones. In contrast to relaxin- 1 and - 2, whose main functions are associated with pregnancy, relaxin- 3 is...

Structural and functional characterization of the conserved salt bridge in mammalian Paneth cell alpha-defensins - Solution structures of mouse cryptdin-4 AND (E15D)-cryptdin-4 (2006)

Rosengren, K. J., Daly, N. L., Fornander, M., Jonsson, M. H., Shirafuji, Y., Qu, X. Q., ...

alpha-Defensins are mediators of mammalian innate immunity, and knowledge of their structure-function relationships is essential for understanding their mechanisms of action. We report here the NMR...

Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design (2006)

Clark, R. J., Daly, N. L., Craik, D. J.

The cyclotide family of plant proteins is of interest because of their unique topology, which combines a head-to-tail cyclic backbone with an embedded cystine knot, and because their-remarkable...

The absolute structural requirement for a proline in the P3 '-position of Bowman-Birk protease inhibitors is surmounted in the minimized SFTI-1 scaffold (2006)

Daly, N. L., Chen, Y. K., Foley, F. M., Bansal, P. S., Bharathi, R., Clark, R. J., ...

SFTI-1 is a small cyclic peptide from sunflower seeds that is one of the most potent trypsin inhibitors of any naturally occurring peptide and is related to the Bowman-Birk family of inhibitors...

The cyclotide family of circular miniproteins: Nature's combinatorial peptide template (2006)

Craik, D. J., Cemazar, M., Wang, C. K. L., Daly, N. L.

The cyclotides are a recently discovered family of miniproteins that contain a head-to-tail cyclized backbone and a knotted arrangement of disulfide bonds. They are approximately 30 amino acids in...

The cyclotides and related macrocyclic peptides as scaffolds in drug design (2006)

Craik, D. J., Cemazar, M., Daly, N. L.

The applicability of linear peptides as drugs is potentially limited by their susceptibility to proteolytic cleavage and poor bioavailability. Cyclotides are macrocyclic cystine-knotted mini-proteins...

theta-defensins prevent HIV-1 Env-mediated fusion by binding gp41 and blocking 6-helix bundle formation (2006)

Gallo, S. A., Wang, W., Rawat, S. S., Jung, G., Waring, A. J., Cole, A. M., ...

Retrocyclin-1, a 0-defensin, protects target cells from human immunodeficiency virus, type 1 (HIV-1) by preventing viral entry. To delineate its mechanism, we conducted fusion assays between...

Chemical synthesis and structure elucidation of bovine K-casein (1-44) (2006)

Bansal, P.S., Daly, N.L., McGhie, E., Craik, D.J., Alewood, P.F., Grieve, P., ...

The caseins (αs1, αs2, β, and κ) are phosphoproteins present in bovine milk that have been studied for over a century and whose structures remain obscure. Here we describe the chemical synthesis...

Chemical synthesis and structure elucidation of bovine K-casein (1-44) (2006)

Bansal, P.S., Daly, N.L., McGhie, E., Craik, D.J., Alewood, P.F., Grieve, P., ...

The caseins (αs1, αs2, β, and κ) are phosphoproteins present in bovine milk that have been studied for over a century and whose structures remain obscure. Here we describe the chemical synthesis...

Chemical synthesis and structure elucidation of bovine K-casein (1-44) (2006)

Bansal, P.S., Daly, N.L., McGhie, E., Craik, D.J., Alewood, P.F., Grieve, P., ...

The caseins (αs1, αs2, β, and κ) are phosphoproteins present in bovine milk that have been studied for over a century and whose structures remain obscure. Here we describe the chemical synthesis...

Solution structure of chi-conopeptide MrIA, a modulator of the human norepinephrine transporter (2005)

Nilsson, K. P. R., Lovelace, E. S., Caesar, C. E., Tynngard, N., Alewood, P. F., Johansson, H. M., ...

The chi-conopeptides MrIA and MrIB are 13-residue peptides with two disulfide bonds that inhibit human and rat norepinephrine transporter systems and are of significant interest for the design of...

Engineering stable peptide toxins by means of backbone cyclization: Stabilization of the alpha-conotoxin MII (2005)

Clark, R. J., Fischer, H., Dempster, L., Daly, N. L., Rosengren, K. J., Nevin, S. T., ...

Conotoxins (CTXs), with their exquisite specificity and potency, have recently created much excitement as drug leads. However, like most peptides, their beneficial activities may potentially be...

Structure of circulin B and implications for antimicrobial activity of the cyclotides (2005)

Koltay, A., Daly, N. L., Gustafson, K. R., Craik, D. J.

The solution structure of one of the first members of the cyclotide family of macrocyclic peptides to be discovered, circulin B has been determined and compared with that of circulin A and related...

Solution structure of chi-conopeptide MrIA, a modulator of the human norepinephrine transporter (2005)

Nilsson, K. P. R., Lovelace, E. S., Caesar, C. E., Tynngard, N., Alewood, P. F., Johansson, H. M., ...

The chi-conopeptides MrIA and MrIB are 13-residue peptides with two disulfide bonds that inhibit human and rat norepinephrine transporter systems and are of significant interest for the design of...

Engineering stable peptide toxins by means of backbone cyclization: Stabilization of the alpha-conotoxin MII (2005)

Clark, R. J., Fischer, H., Dempster, L., Daly, N. L., Rosengren, K. J., Nevin, S. T., ...

Conotoxins (CTXs), with their exquisite specificity and potency, have recently created much excitement as drug leads. However, like most peptides, their beneficial activities may potentially be...

Structure of circulin B and implications for antimicrobial activity of the cyclotides (2005)

Koltay, A., Daly, N. L., Gustafson, K. R., Craik, D. J.

The solution structure of one of the first members of the cyclotide family of macrocyclic peptides to be discovered, circulin B has been determined and compared with that of circulin A and related...

Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins (2004)

Dutton, J. L., Renda, R. F., Waine, C., Clark, R. J., Daly, N. L., Jennings, C. V., ...

The cyclotides are the largest family of naturally occurring circular proteins. The mechanism by which the termini of these gene-encoded proteins are linked seamlessly with a peptide bond to form a...

Structures of mu O-conotoxins from Conus marmoreus - Inhibitors of tetrodotoxin (TTX)-sensitive and TTX-resistant sodium channels in mammalian sensory neurons (2004)

Daly, N. L., Ekberg, J. A., Thomas, L., Adams, D. J., Lewis, R. J., Craik, D. J.

The muO-conotoxins are an intriguing class of conotoxins targeting various voltage-dependent sodium channels and molluscan calcium channels. In the current study, we have shown MrVIA and MrVIB to be...

A comparison of the self-association behavior of the plant cyclotides kalata B1 and kalata B2 via analytical ultracentrifugation (2004)

Nourse, A., Trabi, M., Daly, N. L., Craik, D. J.

The recently discovered cyclotides kalata B1 and kalata B2 are miniproteins containing a head-to-tail cyclized backbone and a cystine knot motif, in which disulfide bonds and the connecting backbone...

Structure-activity relationships of alpha-conotoxins targeting neuronal nicotinic acetylcholine receptors (2004)

Millard, E. L., Daly, N. L., Craik, DJ

alpha-Conotoxins that target the neuronal nicotinic acetylcholine receptor have a range of potential therapeutic applications and are valuable probes for examining receptor subtype selectivity. The...

Solution structure of the cyclotide palicourein: Implications for the development of a pharmaceutical framework (2004)

Barry, D. G., Daly, N. L., Bokesch, H. R., Gustafson, K. R., Craik, D. J.

The cyclotides are a family of disulfide-rich proteins from plants. They have the characteristic structural features of a circular protein backbone and a knotted arrangement of disulfide bonds....

Discovery, structure and biological activities of the cyclotides (2004)

Craik, D. J., Daly, N. L., Mulvenna, J., Plan, M. R., Trabi, M.

The cyclotides are a family of small disulfide rich proteins that have a cyclic peptide backbone and a cystine knot formed by three conserved disulfide bonds. The combination of these two structural...

A comparison of the self-association behavior of the plant cyclotides kalata B1 and kalata B2 via analytical ultracentrifugation (2004)

Nourse, A., Trabi, M., Daly, N. L., Craik, D. J.

The recently discovered cyclotides kalata B1 and kalata B2 are miniproteins containing a head-to-tail cyclized backbone and a cystine knot motif, in which disulfide bonds and the connecting backbone...

Structure-activity relationships of alpha-conotoxins targeting neuronal nicotinic acetylcholine receptors (2004)

Millard, E. L., Daly, N. L., Craik, D. J.

alpha-Conotoxins that target the neuronal nicotinic acetylcholine receptor have a range of potential therapeutic applications and are valuable probes for examining receptor subtype selectivity. The...

Solution structure of the cyclotide palicourein: Implications for the development of a pharmaceutical framework (2004)

Barry, D. G., Daly, N. L., Bokesch, H. R., Gustafson, K. R., Craik, D. J.

The cyclotides are a family of disulfide-rich proteins from plants. They have the characteristic structural features of a circular protein backbone and a knotted arrangement of disulfide bonds....

Discovery, structure and biological activities of the cyclotides (2004)

Craik, D. J., Daly, N. L., Mulvenna, J., Plan, M. R., Trabi, M.

The cyclotides are a family of small disulfide rich proteins that have a cyclic peptide backbone and a cystine knot formed by three conserved disulfide bonds. The combination of these two structural...

Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins (2004)

Dutton, J. L., Renda, R. F., Waine, C., Clark, R. J., Daly, N. L., Jennings, C. V., ...

The cyclotides are the largest family of naturally occurring circular proteins. The mechanism by which the termini of these gene-encoded proteins are linked seamlessly with a peptide bond to form a...

Structures of mu O-conotoxins from Conus marmoreus - Inhibitors of tetrodotoxin (TTX)-sensitive and TTX-resistant sodium channels in mammalian sensory neurons (2004)

Daly, N. L., Ekberg, J. A., Thomas, L., Adams, D. J., Lewis, R. J., Craik, D. J.

The muO-conotoxins are an intriguing class of conotoxins targeting various voltage-dependent sodium channels and molluscan calcium channels. In the current study, we have shown MrVIA and MrVIB to be...

Disulfide folding pathways of cystine knot proteins (2003)

Daly, N. L., Clark, R. J., Craik, D. J.

The plant cyclotides are a fascinating family of circular proteins that contain a cyclic cystine knot motif. The knotted topology and cyclic nature of the cyclotides pose interesting questions about...

Diversity in the disulfide folding pathways of cystine knot peptides (2003)

Daly, N. L., Clark, R. J., Goransson, U., Craik, D. J.

The plant cyclotides are a fascinating family of circular proteins that contain a cyclic cystine knot motif (CCK). This unique family was discovered only recently but contains over 50 known sequences...

Isolation, Structure, and Activity of GID, a Novel alpha 4/7-Conotoxin with an Extended N-terminal Sequence (2003)

Nicke, A., Loughnan, M. L., Millard, E. L., Alewood, P. F., Adams, D. J., Daly, N. L., ...

Using assay-directed fractionation of Conus geographus crude venom, we isolated alpha -conotoxin GID, which acts selectively at neuronal nicotinic acetylcholine receptors (nAChRs). Unlike other...

Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity (2003)

Barry, D. G., Daly, N. L., Clark, R. J., Sando, L., Craik, D. J.

Cyclotides are a recently discovered family of disulfide rich proteins from plants that contain a circular protein backbone. They are exceptionally stable, as exemplified by their use in native...

Isolation, structure, and activity of GID, a novel alpha 4/7-conotoxin with an extended N-terminal sequence (2003)

Nicke, A., Loughnan, M. L., Millard, E. L., Alewood, P. F., Adams, D. J., Daly, N. L., ...

Using assay-directed fractionation of Conus geographus crude venom, we isolated a-conotoxin GID, which acts selectively at neuronal nicotinic acetylcholine receptors (nAChRs). Unlike other neuronally...

Structure and metal binding studies of the second copper binding domain of the Menkes ATPase (2003)

Jones, C., Daly, N. L., Cobine, P. A., Craik, D. J., Dameron, C.

Biological utilisation of copper requires that the metal, in its ionic forms, be meticulously transported, inserted into enzymes and regulatory proteins, and excess be excreted. To understand the...

Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone (2003)

Rosengren, K. J., Clark, R. J., Daly, N. L., Goransson, U., Jones, A., Craik, D. J.

Microcin J25 is a 21 amino acid bacterial peptide that has potent antibacterial activity against Gram-negative bacteria, resulting from its interaction with RNA polymerase. The peptide was previously...

Twists, knots, and rings in proteins - Structural definition of the cyclotide framework (2003)

Rosengren, K. J., Daly, N. L., Plan, M. R., Waine, C., Craik, D. J.

In recent years an increasing number of miniproteins containing an amide-cyclized backbone have been discovered. The cyclotide family is the largest group of such proteins and is characterized by a...

Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity (2003)

Barry, D. G., Daly, N. L., Clark, R. J., Sando, L., Craik, D. J.

Cyclotides are a recently discovered family of disulfide rich proteins from plants that contain a circular protein backbone. They are exceptionally stable, as exemplified by their use in native...

Disulfide folding pathways of cystine knot proteins (2003)

Daly, N. L., Clark, R. J., Craik, D. J.

The plant cyclotides are a fascinating family of circular proteins that contain a cyclic cystine knot motif. The knotted topology and cyclic nature of the cyclotides pose interesting questions about...

Structure and metal binding studies of the second copper binding domain of the Menkes ATPase (2003)

Jones, C., Daly, N. L., Cobine, P. A., Craik, D. J., Dameron, C.

Biological utilisation of copper requires that the metal, in its ionic forms, be meticulously transported, inserted into enzymes and regulatory proteins, and excess be excreted. To understand the...

Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone (2003)

Rosengren, K. J., Clark, R. J., Daly, N. L., Goransson, U., Jones, A., Craik, D. J.

Microcin J25 is a 21 amino acid bacterial peptide that has potent antibacterial activity against Gram-negative bacteria, resulting from its interaction with RNA polymerase. The peptide was previously...

Twists, knots, and rings in proteins - Structural definition of the cyclotide framework (2003)

Rosengren, K. J., Daly, N. L., Plan, M. R., Waine, C., Craik, D. J.

In recent years an increasing number of miniproteins containing an amide-cyclized backbone have been discovered. The cyclotide family is the largest group of such proteins and is characterized by a...

Diversity in the disulfide folding pathways of cystine knot peptides (2003)

Daly, N. L., Clark, R. J., Goransson, U., Craik, D. J.

The plant cyclotides are a fascinating family of circular proteins that contain a cyclic cystine knot motif (CCK). This unique family was discovered only recently but contains over 50 known sequences...

Isolation, Structure, and Activity of GID, a Novel alpha 4/7-Conotoxin with an Extended N-terminal Sequence (2003)

Nicke, A., Loughnan, M. L., Millard, E. L., Alewood, P. F., Adams, D. J., Daly, N. L., ...

Using assay-directed fractionation of Conus geographus crude venom, we isolated alpha -conotoxin GID, which acts selectively at neuronal nicotinic acetylcholine receptors (nAChRs). Unlike other...

The cyclotides: Novel macrocyclic peptides as scaffolds in drug design (2002)

Craik, D. J., Simonsen, S., Daly, N. L.

Cyclotides are a novel class of circular, disulfide-rich peptides (similar to 30 amino acids) that display a broad range of bioactivities and have exceptionally high stability. Their physical...

Structure and function of plant toxins (with emphasis on cystine knot toxins) (2002)

Craik, D. J., Daly, N. L., Plan, M. R., Salim, A. A., Sando, L.

Plant toxins are substances produced and secreted by plants to defend themselves against predators. In a broad sense, this includes all substances that have a toxic effect on targeted organisms,...

The cyclotides: Novel macrocyclic peptides as scaffolds in drug design (2002)

Craik, D. J., Simonsen, S., Daly, N. L.

Cyclotides are a novel class of circular, disulfide-rich peptides (similar to 30 amino acids) that display a broad range of bioactivities and have exceptionally high stability. Their physical...

Structure and function of plant toxins (with emphasis on cystine knot toxins) (2002)

Craik, D. J., Daly, N. L., Plan, M. R., Salim, A. A., Sando, L.

Plant toxins are substances produced and secreted by plants to defend themselves against predators. In a broad sense, this includes all substances that have a toxic effect on targeted organisms,...

Circular proteins in plants - Solution structure of a novel macrocyclic trypsin inhibitor from Momordica cochinchinensis (2001)

Felizmenio-Quimio, M. E., Daly, N. L., Craik, D. J.

Much interest has been generated by recent reports on the discovery of circular (i.e. head-to-tail cyclized) proteins in plants. Here we report the three-dimensional structure of one of the newest...

The cystine knot motif in toxins and implications for drug design (2001)

Craik, D. J., Daly, N. L., Waine, C.

The cystine knot structural motif is present in peptides and proteins from a variety of species, including fungi, plants, marine molluscs. insects and spiders. It comprises an embedded ring formed by...

Solution structure of BSTI: A new trypsin inhibitor from skin secretions of Bombina bombina (2001)

Rosengren, K. J., Daly, N. L., Scanlon, M. J., Craik, D. J.

The three-dimensional solution structure of BSTI, a trypsin inhibitor from the European frog Bombina bombina, has been solved using H-1 NMR spectroscopy. The 60 amino acid protein contains five...

Circular proteins in plants - Solution structure of a novel macrocyclic trypsin inhibitor from Momordica cochinchinensis (2001)

Felizmenio-Quimio, M. E., Daly, N. L., Craik, D. J.

Much interest has been generated by recent reports on the discovery of circular (i.e. head-to-tail cyclized) proteins in plants. Here we report the three-dimensional structure of one of the newest...

The cystine knot motif in toxins and implications for drug design (2001)

Craik, D. J., Daly, N. L., Waine, C.

The cystine knot structural motif is present in peptides and proteins from a variety of species, including fungi, plants, marine molluscs. insects and spiders. It comprises an embedded ring formed by...

Solution structure of BSTI: A new trypsin inhibitor from skin secretions of Bombina bombina (2001)

Rosengren, K. J., Daly, N. L., Scanlon, M. J., Craik, D. J.

The three-dimensional solution structure of BSTI, a trypsin inhibitor from the European frog Bombina bombina, has been solved using H-1 NMR spectroscopy. The 60 amino acid protein contains five...

Role of phosphorylation in the conformation of tau peptides implicated in Alzheimer's disease (2000)

Daly, N. L., Hoffmann, R., Otvos, L. J., Craik, D. J.

A series of peptides corresponding to isolated regions of Tau (tau) protein have been synthesized and their conformations determined by H-1 NMR spectroscopy. Immunodominant peptides corresponding to...

Role of phosphorylation in the conformation of tau peptides implicated in Alzheimer's disease (2000)

Daly, N. L., Hoffmann, R., Otvos, L. J., Craik, D. J.

A series of peptides corresponding to isolated regions of Tau (tau) protein have been synthesized and their conformations determined by H-1 NMR spectroscopy. Immunodominant peptides corresponding to...

Chemical synthesis and folding pathways of large cyclic polypeptide: Studies of the cystine knot polypeptide kalata B1 (1999)

Daly, N. L., Love, S., Alewood, P. F., Craik, D. J.

Kalata B1 is a member of a new family of polypeptides, isolated from. plants, which have a cystine knot structure embedded within an amide-cyclized backbone. This family of molecules are the largest...

Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif (1999)

Craik, D. J., Daly, N. L., Bond, T. J., Waine, C.

Several macrocyclic peptides (similar to 30 amino acids), with diverse biological activities, have been isolated from the Rubiaceae and Violaceae plant families over recent years. We have...

Solution structure of alpha-conotoxin ImI by H-1 nuclear magnetic resonance (1999)

Gehrmann, J., Daly, N. L., Alewood, P. F., Craik, D. J.

alpha-Conotoxin ImI derives from the venom of Conus imperialis and is the first and only small-peptide ligand that selectively binds to the neuronal alpha(7) homopentameric subtype of the nicotinic...

Chemical synthesis and folding pathways of large cyclic polypeptide: Studies of the cystine knot polypeptide kalata B1 (1999)

Daly, N. L., Love, S., Alewood, P. F., Craik, D. J.

Kalata B1 is a member of a new family of polypeptides, isolated from. plants, which have a cystine knot structure embedded within an amide-cyclized backbone. This family of molecules are the largest...

Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif (1999)

Craik, D. J., Daly, N. L., Bond, T. J., Waine, C.

Several macrocyclic peptides (similar to 30 amino acids), with diverse biological activities, have been isolated from the Rubiaceae and Violaceae plant families over recent years. We have...

Three-dimensional structure of a cysteine-rich repeat from the low-density lipoprotein receptor.

Daly, N L, Scanlon, M J, Djordjevic, J T, Kroon, P A, Smith, R

The low-density lipoprotein (LDL) receptor plays a central role in mammalian cholesterol metabolism, clearing lipoproteins which bear apolipoproteins E and B-100 from plasma. Mutations in this...

Three-dimensional structure of a cysteine-rich repeat from the low-density lipoprotein receptor.

Daly, N L, Scanlon, M J, Djordjevic, J T, Kroon, P A, Smith, R

The low-density lipoprotein (LDL) receptor plays a central role in mammalian cholesterol metabolism, clearing lipoproteins which bear apolipoproteins E and B-100 from plasma. Mutations in this...