Scientific Commons: N. P. Minton

Gene cloning in Clostridia (2005)

Davis, I., Carter, G., Young, Michael, Minton, N. P.

Modifying an immunogenic epitope on a therapeutic protein: a step towards an improved system for antibody-directed enzyme prodrug therapy (ADEPT) (2004)

Mayer, A., Sharma, S.K., Tolner, B., Minton, N.P., Purdy, D., Amlot, P., ...

Carboxypeptidase G2 (CP) is a bacterial enzyme, which is targeted to tumours by an antitumour antibody for local prodrug activation in antibody-directed enzyme prodrug therapy (ADEPT). Repeated...

A strategy for mapping and neutralizing conformational immunogenic sites on protein therapeutics (2002)

Spencer, D.I.R., Robson, L., Purdy, D., Whitelegg, N.R., Michael, N.P., Bhatia, J., ...

Antibodies are highly specific recognition molecules which are increasingly being applied to target therapy in patients. One type of developmental antibody-based therapy is antibody directed enzyme...

Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence.

Whelan, S M, Elmore, M J, Bodsworth, N J, Brehm, J K, Atkinson, T, Minton, N P

DNA fragments derived from the Clostridium botulinum type A neurotoxin (BoNT/A) gene (botA) were used in DNA-DNA hybridization reactions to derive a restriction map of the region of the C. botulinum...

A simple procedure for gel electrophoresis and northern blotting of RNA.

Goda, S K, Minton, N P

The gram-positive cloning vector pBD64 arose by a 1844 bp deletion of pC194 derived DNA.

Minton, N P, Swinfield, T J, Brehm, J K, Oultram, J D

Nucleotide sequence of an Erwinia chrysanthemi gene encoding shikimate kinase.

Minton, N P, Whitehead, P J, Atkinson, T, Gilbert, H J

The replication proteins of plasmids pE194 and pLS1 have N-terminal homology.

Minton, N P, Oultram, J D, Brehm, J K, Atkinson, T

Nucleotide sequence of the succinyl-CoA synthetase alpha-subunit from Thermus aquaticus B.

Nicholls, D J, Sundaram, T K, Atkinson, T, Minton, N P

Complete nucleotide sequence and deduced amino acid sequence of the M5 polypeptide gene of Escherichia coli.

Kemp, E H, Minton, N P, Mann, N H

Regeneration of protoplasts of Clostridium pasteurianum ATCC 6013.

Minton, N P, Morris, J G

A procedure has been developed for the regeneration of Clostridium pasteurianum protoplasts with frequencies of up to 10% reversion being obtained.

Molecular cloning of the Pseudomonas carboxypeptidase G2 gene and its expression in Escherichia coli and Pseudomonas putida.

Minton, N P, Atkinson, T, Sherwood, R F

The gene coding for carboxypeptidase G2 was cloned from Pseudomonas sp. strain RS-16 into Escherichia coli W5445 by inserting Sau3A-generated DNA fragments into the BamHI site of pBR322. The plasmid...