N. S. Huskisson

Publication List Details

Number

18

Co-Authors

Stress signaling in plants: a mitogen-activated protein kinase pathway is activated by cold and drought.

Jonak, C, Kiegerl, S, Ligterink, W, Barker, P J, Huskisson, N S, Hirt, H

Yeast and animals use mitogen-activated protein (MAP) kinase cascades to mediate stress and extracellular signals. We have tested whether MAP kinases are involved in mediating environmental stress...

A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division.

Bögre, L, Calderini, O, Binarova, P, Mattauch, M, Till, S, Kiegerl, S, ...

In eukaryotes, mitogen-activated protein kinases (MAPKs) are part of signaling modules that transmit diverse stimuli, such as mitogens, developmental cues, or various stresses. Here, we report a...

Purification and characterization of a new endoglucanase from Clostridium thermocellum.

Romaniec, M P, Fauth, U, Kobayashi, T, Huskisson, N S, Barker, P J, Demain, A L

An endoglucanase (1,4-beta-D-glucan glucanohydrolase, EC 3.2.1.4) from the thermophilic anaerobe Clostridium thermocellum was purified to apparent homogeneity without the use of denaturants. No...

Binding of gelatinases A and B to type-I collagen and other matrix components.

Allan, J A, Docherty, A J, Barker, P J, Huskisson, N S, Reynolds, J J, Murphy, G

Matrix sequestration of matrix metalloproteinases may be important for the facilitation of remodelling events and the migration of cells through the extracellular matrix. Using an ELISA technique we...

The design and construction of an oxygen micro-electrode for monitoring 20-100 microliter volumes.

Huskisson, N S, Ward, P F, Munn, E A

Full instructions are given for the construction of a micro-respirometer. It is based on a membrane-covered platinum electrode of 10 micron diameter that consumes little oxygen. The reaction chamber,...

The metabolism of fluoroacetate in lettuce

Ward, P. F. V., Huskisson, N. S.

1. Whole lettuce plants were incubated with (1) [1-14C]acetate, (2) fluoroacetate followed by [1-14C]acetate, (3) fluoro[1-14C]acetate, (4) fluoro[2-14C]acetate or (5)...

Stress signaling in plants: a mitogen-activated protein kinase pathway is activated by cold and drought.

Jonak, C, Kiegerl, S, Ligterink, W, Barker, P J, Huskisson, N S, Hirt, H

Yeast and animals use mitogen-activated protein (MAP) kinase cascades to mediate stress and extracellular signals. We have tested whether MAP kinases are involved in mediating environmental stress...

A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division.

Bögre, L, Calderini, O, Binarova, P, Mattauch, M, Till, S, Kiegerl, S, ...

In eukaryotes, mitogen-activated protein kinases (MAPKs) are part of signaling modules that transmit diverse stimuli, such as mitogens, developmental cues, or various stresses. Here, we report a...

Purification and characterization of a new endoglucanase from Clostridium thermocellum.

Romaniec, M P, Fauth, U, Kobayashi, T, Huskisson, N S, Barker, P J, Demain, A L

An endoglucanase (1,4-beta-D-glucan glucanohydrolase, EC 3.2.1.4) from the thermophilic anaerobe Clostridium thermocellum was purified to apparent homogeneity without the use of denaturants. No...

Binding of gelatinases A and B to type-I collagen and other matrix components.

Allan, J A, Docherty, A J, Barker, P J, Huskisson, N S, Reynolds, J J, Murphy, G

Matrix sequestration of matrix metalloproteinases may be important for the facilitation of remodelling events and the migration of cells through the extracellular matrix. Using an ELISA technique we...

The design and construction of an oxygen micro-electrode for monitoring 20-100 microliter volumes.

Huskisson, N S, Ward, P F, Munn, E A

Full instructions are given for the construction of a micro-respirometer. It is based on a membrane-covered platinum electrode of 10 micron diameter that consumes little oxygen. The reaction chamber,...

The metabolism of fluoroacetate in lettuce

Ward, P. F. V., Huskisson, N. S.

1. Whole lettuce plants were incubated with (1) [1-14C]acetate, (2) fluoroacetate followed by [1-14C]acetate, (3) fluoro[1-14C]acetate, (4) fluoro[2-14C]acetate or (5)...

Blot-sequencing of antibodies: application to analysis of V gene usage among anti-steroid monoclonal antibodies

Taussig, M. J., Symington, P. R., Woods, M., Sims, M. J., Beale, D., Humphreys, A. S., ...

Automated gas-phase protein sequencing has been used to characterize variable regions of antibody heavy and light chains separated by SDS—polyacrylamide gel electrophoresis (PAGE) and...

Neuropeptide Y (NPY) metabolism by endopeptidase-2 hinders characterization of NPY receptors in rat kidney.

Price, J. S., Kenny, A. J., Huskisson, N. S., Brown, M. J.

1. Despite the observation of pharmacological responses to neuropeptide Y (NPY) in mammalian kidneys, there are species differences in the ease with which specific NPY binding sites can be...