Control of eIF4E cellular localization by eIF4E-binding proteins, 4E-BPs (2008)
Rong, Liwei, Livingstone, Mark, Sukarieh, Rami, Petroulakis, Emmanuel, Gingras, Anne-Claude, Crosby, Katherine, ...
Eukaryotic initiation factor (eIF) 4E, the mRNA 5′-cap-binding protein, mediates the association of eIF4F with the mRNA 5′-cap structure to stimulate cap-dependent translation initiation in the...
Weak binding affinity of human 4EHP for mRNA cap analogs (2007)
Zuberek, Joanna, Kubacka, Dorota, Jablonowska, Agnieszka, Jemielity, Jacek, Stepinski, Janusz, Sonenberg, Nahum, ...
Ribosome recruitment to the majority of eukaryotic mRNAs is facilitated by the interaction of the cap binding protein, eIF4E, with the mRNA 5′ cap structure. eIF4E stimulates translation through...
Svitkin, Yuri V., Costa-Mattioli, Mauro, Herdy, Barbara, Perreault, Sandra, Sonenberg, Nahum
Picornavirus infectivity is dependent on the RNA poly(A) tail, which binds the poly(A) binding protein (PABP). PABP was reported to stimulate viral translation and RNA synthesis. Here, we studied...
Weak binding affinity of human 4EHP for mRNA cap analogs (2007)
Zuberek, Joanna, Kubacka, Dorota, Jablonowska, Agnieszka, Jemielity, Jacek, Stepinski, Janusz, Sonenberg, Nahum, ...
Ribosome recruitment to the majority of eukaryotic mRNAs is facilitated by the interaction of the cap binding protein, eIF4E, with the mRNA 5′ cap structure. eIF4E stimulates translation through...
Berlanga, Juan José, Baass, Alexis, Sonenberg, Nahum
The 5′ cap and 3′ poly(A) tail of eukaryotic mRNAs act synergistically to enhance translation. This synergy is mediated via interactions between eIF4G (a component of the eIF4F cap binding...
Berlanga, Juan José, Baass, Alexis, Sonenberg, Nahum
The 5′ cap and 3′ poly(A) tail of eukaryotic mRNAs act synergistically to enhance translation. This synergy is mediated via interactions between eIF4G (a component of the eIF4F cap binding...
Miyakawa, Shin, Oguro, Akihiro, Ohtsu, Takashi, Imataka, Hiroaki, Sonenberg, Nahum, Nakamura, Yoshikazu
Eukaryotic translation initiation factor 4G (eIF4G) plays a crucial multimodulatory role in mRNA translation and decay by interacting with other translation factors and mRNA-associated proteins. In...
Miyakawa, Shin, Oguro, Akihiro, Ohtsu, Takashi, Imataka, Hiroaki, Sonenberg, Nahum, Nakamura, Yoshikazu
Eukaryotic translation initiation factor 4G (eIF4G) plays a crucial multimodulatory role in mRNA translation and decay by interacting with other translation factors and mRNA-associated proteins. In...
Berlanga, Juan José, Baass, Alexis, Sonenberg, Nahum
The 5′ cap and 3′ poly(A) tail of eukaryotic mRNAs act synergistically to enhance translation. This synergy is mediated via interactions between eIF4G (a component of the eIF4F cap binding...
Miyakawa, Shin, Oguro, Akihiro, Ohtsu, Takashi, Imataka, Hiroaki, Sonenberg, Nahum, Nakamura, Yoshikazu
Eukaryotic translation initiation factor 4G (eIF4G) plays a crucial multimodulatory role in mRNA translation and decay by interacting with other translation factors and mRNA-associated proteins. In...
Bordeleau, Marie-Eve, Matthews, James, Wojnar, Joanna M., Lindqvist, Lisa, Novac, Olivia, Jankowsky, Eckhard, ...
RNA helicases are the largest group of enzymes in eukaryotic RNA metabolism. The DEXD/H-box putative RNA helicases form the helicase superfamily II, whose members are defined by seven highly...
Tettweiler, Gritta, Miron, Mathieu, Jenkins, Mark, Sonenberg, Nahum, Lasko, Paul F.
eIF4E, the mRNA 5′ cap-binding protein, is regulated by its binding protein (4E-BP), a downstream target of phosphatidylinositol-3-OH kinase [PI(3)K] signaling. We show that Drosophila 4E-BP...
Tettweiler, Gritta, Miron, Mathieu, Jenkins, Mark, Sonenberg, Nahum, Lasko, Paul F.
eIF4E, the mRNA 5′ cap-binding protein, is regulated by its binding protein (4E-BP), a downstream target of phosphatidylinositol-3-OH kinase [PI(3)K] signaling. We show that Drosophila 4E-BP...
MOCHIZUKI, KIYOTAKA, OGURO, AKIHIRO, OHTSU, TAKASHI, SONENBERG, NAHUM, NAKAMURA, YOSHIKAZU
The eukaryotic translation initiation factor 4F (eIF4F) consists of three polypeptides (eIF4A, eIF4G, and eIF4E) and is responsible for recruiting ribosomes to mRNA. eIF4E recognizes the mRNA...
Kahvejian, Avak, Svitkin, Yuri V., Sukarieh, Rami, M'Boutchou, Marie-Noël, Sonenberg, Nahum
Translation initiation is a multistep process involving several canonical translation factors, which assemble at the 5′-end of the mRNA to promote the recruitment of the ribosome. Although the 3′...
Tettweiler, Gritta, Miron, Mathieu, Jenkins, Mark, Sonenberg, Nahum, Lasko, Paul F.
eIF4E, the mRNA 5′ cap-binding protein, is regulated by its binding protein (4E-BP), a downstream target of phosphatidylinositol-3-OH kinase [PI(3)K] signaling. We show that Drosophila 4E-BP...
Upstream and downstream of mTOR (2004)
The evolutionarily conserved checkpoint protein kinase, TOR (target of rapamycin), has emerged as a major effector of cell growth and proliferation via the regulation of protein synthesis. Work in...
Chang, Tsung-Cheng, Yamashita, Akio, Chen, Chyi-Ying A., Yamashita, Yukiko, Zhu, Wenmiao, Durdan, Simon, ...
Messenger RNA decay mediated by the c-fos major protein coding-region determinant of instability (mCRD) is a useful system for studying translationally coupled mRNA turnover. Among the five...
Yang, Hsin-Sheng, Jansen, Aaron P., Komar, Anton A., Zheng, Xiaojing, Merrick, William C., Costes, Sylvain, ...
Pdcd4 is a novel transformation suppressor that inhibits tumor promoter-induced neoplastic transformation and the activation of AP-1-dependent transcription required for transformation. A yeast...
OGURO, AKIHIRO, OHTSU, TAKASHI, SVITKIN, YURI V., SONENBERG, NAHUM, NAKAMURA, YOSHIKAZU
The mammalian translation initiation factor 4A (eIF4A) is a prototype member of the DEAD-box RNA helicase family that couples ATPase activity to RNA binding and unwinding. In the crystal form, eIF4A...
ZUBEREK, JOANNA, WYSLOUCH-CIESZYNSKA, ALEKSANDRA, NIEDZWIECKA, ANNA, DADLEZ, MICHAL, STEPINSKI, JANUSZ, AUGUSTYNIAK, WOJCIECH, ...
Phosphorylation of the eukaryotic initiation factor eIF4E in response to mitogenic stimuli and cytokines is implicated in the regulation of the initiation step of translation. It still remains...
Tang, Shao Jun, Reis, Gerald, Kang, Hyejin, Gingras, Anne-Claude, Sonenberg, Nahum, Schuman, Erin M.
Many forms of long-lasting behavioral and synaptic plasticity require the synthesis of new proteins. For example, long-term potentiation (LTIP) that endures for more than an hour requires both...
Hierarchical phosphorylation of the translation inhibitor 4E-BP1 (2001)
Gingras, Anne-Claude, Raught, Brian, Gygi, Steven P., Niedzwiecka, Anna, Miron, Mathieu, Burley, Stephen K., ...
Li, Qiyu, Imataka, Hiroaki, Morino, Shigenobu, Rogers, George W., Richter-Cook, Nancy J., Merrick, William C., ...
Eukaryotic initiation factor 4A (eIF4A) is an RNA-dependent ATPase and ATP-dependent RNA helicase that is thought to melt the 5' proximal secondary structure of eukaryotic mRNAs to facilitate...
Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism (1999)
Gingras, Anne-Claude, Gygi, Steven P., Raught, Brian, Polakiewicz, Roberto D., Abraham, Robert T., Hoekstra, Merl F., ...
Le, Shu-Yun, Chen, Jih-H., Sonenberg, Nahum, Maizel, Jacob V.
Statistical analyses of RNA folding In 5′ nontranslated regions (5’NTR) of encephalomyocarditis virus, Theller's murlne encephalomyelltls virus, foot-and-mouth disease virus, and hepatitis A...
Identification of nuclear cap specific proteins in HeLa cells (1987)
Rozen, Florence, Sonenberg, Nahum
Two polypeptides of apparent molecular mass of 20 and 115 kilodaltons in nuclear fractions from HeLa cells were shown to recognize and be crosslinked to the cap structure of eukaryotic mRNAs in a...
ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA (1981)
Two proteins of apparent molecular weights of 28,000 and 50,000 daltons were shown to recognize and cross-link specifically to the 5′ cap end of oxidized reovirus mRNA. Cross-linking of these...
Sonenberg, Nahum, Morgan, Maureen A., Testa, Douglas, Colonno, Richard J., Shatkin, Aaron J.
A variety of 5′- 3H-methyl-labeled, oxidized viral mRNAs were used as probes for detecting in wheat germ initiation complexes proteins that interact with, and can be cross-linked to, the 5′-cap...
Analysis of terminal structures of RNA from potato virus X (1978)
Sonenberg, Nahum, Shatkin, Aaron J., Ricciardi, Robert P., Rubin, Michal, Goodman, Robert M.
The 5′-end structure of potato virus X RNA was determined following enzymatic methylation in vitro A single 3H group was introduced into the 2′-position of the 5′-penultimate residue and the...
Karim, Muhammad M., Svitkin, Yuri V., Kahvejian, Avak, De Crescenzo, Gregory, Costa-Mattioli, Mauro, Sonenberg, Nahum
The eukaryotic mRNA 3′ poly(A) tail and the 5′ cap cooperate to synergistically enhance translation. This interaction is mediated by the cap-binding protein eIF4E, the poly(A) binding protein...
Gradi, Alessandra, Svitkin, Yuri V., Imataka, Hiroaki, Sonenberg, Nahum
Eukaryotic initiation factor (eIF) 4GI is a component of the cap-binding protein complex eIF4F, which is required for cap-dependent translation. Infection of cells by poliovirus results in a...
Deo, Rahul C., Sonenberg, Nahum, Burley, Stephen K.
The poly(A)-binding protein (PABP) recognizes the 3′ mRNA poly(A) tail and plays an essential role in eukaryotic translation initiation and mRNA stabilization/degradation. PABP is a modular...
The target of rapamycin (TOR) proteins
Raught, Brian, Gingras, Anne-Claude, Sonenberg, Nahum
Rapamycin potently inhibits downstream signaling from the target of rapamycin (TOR) proteins. These evolutionarily conserved protein kinases coordinate the balance between protein synthesis and...
FRAP/mTOR is required for proliferation and patterning during embryonic development in the mouse
Hentges, Kathryn E., Sirry, Baheya, Gingeras, Anne-Claude, Sarbassov, Dos, Sonenberg, Nahum, Sabatini, David, ...
The FKBP-12-rapamycin associated protein (FRAP, also known as mTOR and RAFT-1) is a member of the phosphoinositide kinase related kinase family. FRAP has serine/threonine kinase activity and mediates...
Li, Qiyu, Imataka, Hiroaki, Morino, Shigenobu, Rogers, George W., Richter-Cook, Nancy J., Merrick, William C., ...
Eukaryotic initiation factor 4A (eIF4A) is an RNA-dependent ATPase and ATP-dependent RNA helicase that is thought to melt the 5′ proximal secondary structure of eukaryotic mRNAs to facilitate...
Morino, Shigenobu, Imataka, Hiroaki, Svitkin, Yuri V., Pestova, Tatyana V., Sonenberg, Nahum
The mammalian eukaryotic initiation factor 4GI (eIF4GI) may be divided into three roughly equal regions; an amino-terminal one-third (amino acids [aa] 1 to 634), which contains the poly(A) binding...
Poliovirus 2A Protease Induces Apoptotic Cell Death
Goldstaub, Dan, Gradi, Alessandra, Bercovitch, Zippi, Grosmann, Zehava, Nophar, Yaron, Luria, Sylvie, ...
A cell line was generated that expresses the poliovirus 2A protease in an inducible manner. Tightly controlled expression was achieved by utilizing the muristerone A-regulated expression system. Upon...
Cosentino, Gregory P., Schmelzle, Tobias, Haghighat, Ashkan, Helliwell, Stephen B., Hall, Michael N., Sonenberg, Nahum
Ribosome binding to eukaryotic mRNA is a multistep process which is mediated by the cap structure [m7G(5′)ppp(5′)N, where N is any nucleotide] present at the 5′ termini of all cellular (with...
Dual Interactions of the Translational Repressor Paip2 with Poly(A) Binding Protein
Khaleghpour, Kianoush, Kahvejian, Avak, De Crescenzo, Gregory, Roy, Guylaine, Svitkin, Yuri V., Imataka, Hiroaki, ...
The cap structure and the poly(A) tail of eukaryotic mRNAs act synergistically to enhance translation. This effect is mediated by a direct interaction of eukaryotic initiation factor 4G and poly(A)...
Mazumder, Barsanjit, Seshadri, Vasudevan, Imataka, Hiroaki, Sonenberg, Nahum, Fox, Paul L.
Ceruloplasmin (Cp) is a glycoprotein secreted by the liver and monocytic cells and probably plays roles in inflammation and iron metabolism. We showed previously that gamma interferon (IFN-γ)...
Svitkin, Yuri V., Gradi, Alessandra, Imataka, Hiroaki, Morino, Shigenobu, Sonenberg, Nahum
For many members of the Picornaviridae family, infection of cells results in a shutoff of host protein synthesis. For rhinoviruses and enteroviruses, the shutoff has been explained in part by the...
Khaleghpour, Kianoush, Pyronnet, Stéphane, Gingras, Anne-Claude, Sonenberg, Nahum
Eukaryotic translation initiation factor 4E (eIF4E) is the mRNA 5′ cap binding protein, which plays an important role in the control of translation. The activity of eIF4E is regulated by a family...
Rapamycin and Wortmannin Enhance Replication of a Defective Encephalomyocarditis Virus
Svitkin, Yuri V., Hahn, Harry, Gingras, Anne-Claude, Palmenberg, Ann C., Sonenberg, Nahum
Inhibitors of the phosphatidylinositol 3-kinase (PI3 kinase)–FKBP-rapamycin-associated protein (FRAP) pathway, such as rapamycin and wortmannin, induce dephosphorylation and activation of the...
Stojdl, David F., Abraham, Ninan, Knowles, Shane, Marius, Ricardo, Brasey, Ann, Lichty, Brian D., ...
Interferon (IFN)-induced antiviral responses are mediated through a variety of proteins, including the double-stranded RNA-dependent protein kinase PKR. Here we show that fibroblasts derived from...
Tang, Shao Jun, Reis, Gerald, Kang, Hyejin, Gingras, Anne-Claude, Sonenberg, Nahum, Schuman, Erin M.
Many forms of long-lasting behavioral and synaptic plasticity require the synthesis of new proteins. For example, long-term potentiation (LTP) that endures for more than an hour requires both...
A Novel Functional Human Eukaryotic Translation Initiation Factor 4G
Gradi, Alessandra, Imataka, Hiroaki, Svitkin, Yuri V., Rom, Eran, Raught, Brian, Morino, Shigenobu, ...
Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a cap-binding protein complex consisting of three subunits: eIF4E, eIF4A, and eIF4G. In yeast and plants, two related eIF4G species...
The major mRNA-associated protein YB-1 is a potent 5′ cap-dependent mRNA stabilizer
Evdokimova, Valentina, Ruzanov, Peter, Imataka, Hiroaki, Raught, Brian, Svitkin, Yuri, Ovchinnikov, Lev P., ...
mRNA silencing and storage play an important role in gene expression under diverse circumstances, such as throughout early metazoan development and in response to many types of environmental stress....
Li, Shunan, Sonenberg, Nahum, Gingras, Anne-Claude, Peterson, Mark, Avdulov, Svetlana, Polunovsky, Vitaly A., ...
Translational control has been recently added to well-recognized genomic, transcriptional, and posttranslational mechanisms regulating apoptosis. We previously found that overexpressed eukaryotic...
Paip1 Interacts with Poly(A) Binding Protein through Two Independent Binding Motifs
Roy, Guylaine, De Crescenzo, Gregory, Khaleghpour, Kianoush, Kahvejian, Avak, O'Connor-McCourt, Maureen, Sonenberg, Nahum
The 3′ poly(A) tail of eukaryotic mRNAs plays an important role in the regulation of translation. The poly(A) binding protein (PABP) interacts with eukaryotic initiation factor 4G (eIF4G), a...
Phosphorylation of Eukaryotic Translation Initiation Factor 4E Is Critical for Growth
Lachance, Pascal E. D., Miron, Mathieu, Raught, Brian, Sonenberg, Nahum, Lasko, Paul
Eukaryotic translation initiation factor 4E (eIF4E) binds to the cap structure at the 5′ end of mRNAs and is a critical target for the control of protein synthesis. eIF4E is phosphorylated in many...
Koumenis, Constantinos, Naczki, Christine, Koritzinsky, Marianne, Rastani, Sally, Diehl, Alan, Sonenberg, Nahum, ...
Hypoxia profoundly influences tumor development and response to therapy. While progress has been made in identifying individual gene products whose synthesis is altered under hypoxia, little is known...
Yang, Hsin-Sheng, Jansen, Aaron P., Komar, Anton A., Zheng, Xiaojing, Merrick, William C., Costes, Sylvain, ...
Pdcd4 is a novel transformation suppressor that inhibits tumor promoter-induced neoplastic transformation and the activation of AP-1-dependent transcription required for transformation. A yeast...
Brasey, Ann, Lopez-Lastra, Marcelo, Ohlmann, Theophile, Beerens, Nancy, Berkhout, Ben, Darlix, Jean-Luc, ...
The 5′ leader of the human immunodeficiency virus type 1 (HIV-1) genomic RNA contains highly structured domains involved in key steps of the viral life cycle. These RNA domains inhibit...
A translational rheostat for RFLAT-1 regulates RANTES expression in T lymphocytes
Nikolcheva, Tania, Pyronnet, Stephane, Chou, Szu-yi, Sonenberg, Nahum, Song, An, Clayberger, Carol, ...
Activation of T lymphocytes by specific antigen triggers a 3- to 7-day maturation process. Terminal differentiation begins late after T cell activation and involves expression of effector genes,...
Gradi, Alessandra, Svitkin, Yuri V., Sommergruber, Wolfgang, Imataka, Hiroaki, Morino, Shigenobu, Skern, Tim, ...
Several picornaviruses shut down host cellular protein synthesis by proteolytic cleavage of the eukaryotic initiation factor (eIF) 4GI and eIF4GII isoforms. Viral RNA translation is maintained by a...
Cell-Free Synthesis of Encephalomyocarditis Virus
Svitkin, Yuri V., Sonenberg, Nahum
We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a...
Dostie, Josée, Ferraiuolo, Maria, Pause, Arnim, Adam, Stephen A., Sonenberg, Nahum
The eukaryotic translation initiation factor 4E (eIF4E) plays an important role in the control of cell growth. eIF4E binds to the mRNA 5′ cap structure m7GpppN (where N is any nucleotide), and...
Raught, Brian, Gingras, Anne-Claude, Gygi, Steven P., Imataka, Hiroaki, Morino, Shigenobu, Gradi, Alessandra, ...
The eukaryotic translation initiation factor 4G (eIF4G) proteins play a critical role in the recruitment of the translational machinery to mRNA. The eIF4Gs are phosphoproteins. However, the location...
Signaling from Akt to FRAP/TOR Targets both 4E-BP and S6K in Drosophila melanogaster
Miron, Mathieu, Lasko, Paul, Sonenberg, Nahum
The eIF4E-binding proteins (4E-BPs) interact with translation initiation factor 4E to inhibit translation. Their binding to eIF4E is reversed by phosphorylation of several key Ser/Thr residues. In...
Suppression of cap-dependent translation in mitosis
Pyronnet, Stéphane, Dostie, Josée, Sonenberg, Nahum
Cap-dependent translation is mediated by eIF4F, a protein complex composed of three subunits as follows: eIF4E, which recognizes the mRNA 5′ cap structure; eIF4A, an RNA-helicase; and eIF4G, a...
Hierarchical phosphorylation of the translation inhibitor 4E-BP1
Gingras, Anne-Claude, Raught, Brian, Gygi, Steven P., Niedzwiecka, Anna, Miron, Mathieu, Burley, Stephen K., ...
In most instances, translation is regulated at the initiation phase, when a ribosome is recruited to the 5′ end of an mRNA. The eIF4E-binding proteins (4E-BPs) interdict translation initiation by...
Gingras, Anne-Claude, Kennedy, Scott G., O’Leary, Maura A., Sonenberg, Nahum, Hay, Nissim
Growth factors and hormones activate protein translation by phosphorylation and inactivation of the translational repressors, the eIF4E-binding proteins (4E-BPs), through a wortmannin- and...
Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism
Gingras, Anne-Claude, Gygi, Steven P., Raught, Brian, Polakiewicz, Roberto D., Abraham, Robert T., Hoekstra, Merl F., ...
The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5′ end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5′ cap...
Roy, Guylaine, Miron, Mathieu, Khaleghpour, Kianoush, Lasko, Paul, Sonenberg, Nahum
The 3′ poly(A) tail of eukaryotic mRNAs and the poly(A) binding protein (PABP) play important roles in the regulation of translation. Recently, a human PABP-interacting protein, Paip2, which...
Sonenberg, Nahum, Guertin, Denise, Lee, Kevin A. W.
Extracts from poliovirus-infected HeLa cells were used to study ribosome binding of native and denatured reovirus mRNAs and translation of capped mRNAs with different degrees of secondary structure....
Gradi, Alessandra, Foeger, Nicole, Strong, Rebecca, Svitkin, Yuri V., Sonenberg, Nahum, Skern, Tim, ...
Foot-and-mouth disease virus (FMDV) induces a very rapid inhibition of host cell protein synthesis within infected cells. This is accompanied by the cleavage of the eukaryotic translation initiation...
Ferraiuolo, Maria A., Lee, Chung-Sheng, Ler, Lian Wee, Hsu, Jeanne L., Costa-Mattioli, Mauro, Luo, Ming-Juan, ...
In eukaryotes, a surveillance mechanism known as nonsense-mediated decay (NMD) degrades the mRNA when a premature-termination codon (PTC) is present. NMD requires translation to read the frame of the...
Yang, Hsin-Sheng, Cho, Myung-Haing, Zakowicz, Halina, Hegamyer, Glenn, Sonenberg, Nahum, Colburn, Nancy H.
Αn α-helical MA-3 domain appears in several translation initiation factors, including human eukaryotic translation initiation factor 4G (eIF4G) and DAP-5/NAT1/p97, as well as in the tumor...
Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases
Raught, Brian, Peiretti, Franck, Gingras, Anne-Claude, Livingstone, Mark, Shahbazian, David, Mayeur, Greg L, ...
The eucaryotic translation initiation factor 4B (eIF4B) stimulates the helicase activity of the DEAD box protein eIF4A to unwind inhibitory secondary structure in the 5′ untranslated region of...
Caron, Sandrine, Charon, Martine, Cramer, Elisabeth, Sonenberg, Nahum, Dusanter-Fourt, Isabelle
mRNA translation is mainly regulated at the level of initiation, a process that involves the synergistic action of the 5′ cap structure and the 3′ poly(A) tail at the ends of eukaryotic mRNA. The...
Costa-Mattioli, Mauro, Svitkin, Yuri, Sonenberg, Nahum
Translation of poliovirus and hepatitis C virus (HCV) RNAs is initiated by recruitment of 40S ribosomes to an internal ribosome entry site (IRES) in the mRNA 5′ untranslated region. Translation...
Chang, Tsung-Cheng, Yamashita, Akio, Chen, Chyi-Ying A., Yamashita, Yukiko, Zhu, Wenmiao, Durdan, Simon, ...
Messenger RNA decay mediated by the c-fos major protein coding-region determinant of instability (mCRD) is a useful system for studying translationally coupled mRNA turnover. Among the five...
Baltzis, Dionissios, Qu, Li-Ke, Papadopoulou, Stavroula, Blais, Jaime D., Bell, John C., Sonenberg, Nahum, ...
Phosphorylation of the alpha (α) subunit of the eukaryotic translation initiation factor 2 (eIF2) leads to the inhibition of protein synthesis in response to diverse stress conditions, including...
Deng, Jing, Lu, Phoebe D., Zhang, Yuhong, Scheuner, Donalyn, Kaufman, Randal J., Sonenberg, Nahum, ...
Numerous stressful conditions activate kinases that phosphorylate the alpha subunit of translation initiation factor 2 (eIF2α), thus attenuating mRNA translation and activating a gene expression...
Kahvejian, Avak, Svitkin, Yuri V., Sukarieh, Rami, M'Boutchou, Marie-Noël, Sonenberg, Nahum
Translation initiation is a multistep process involving several canonical translation factors, which assemble at the 5′-end of the mRNA to promote the recruitment of the ribosome. Although the 3′...
Svitkin, Yuri V., Pause, Arnim, Lopez-Lastra, Marcelo, Perreault, Sandra, Sonenberg, Nahum
We developed an in vitro translation extract from Krebs-2 cells that translates the entire open reading frame of the hepatitis C virus (HCV) strain H77 and properly processes the viral protein...
Gorrini, Chiara, Loreni, Fabrizio, Gandin, Valentina, Sala, Leonardo A., Sonenberg, Nahum, Marchisio, Pier Carlo, ...
Fibronectin (FN) is a major matrix protein involved in multiple processes. Little is known about how adhesion to FN affects the translational machinery. We show that in fibroblasts adhesion to FN...
Bordeleau, Marie-Eve, Matthews, James, Wojnar, Joanna M., Lindqvist, Lisa, Novac, Olivia, Jankowsky, Eckhard, ...
RNA helicases are the largest group of enzymes in eukaryotic RNA metabolism. The DEXD/H-box putative RNA helicases form the helicase superfamily II, whose members are defined by seven highly...
Tettweiler, Gritta, Miron, Mathieu, Jenkins, Mark, Sonenberg, Nahum, Lasko, Paul F.
eIF4E, the mRNA 5′ cap-binding protein, is regulated by its binding protein (4E-BP), a downstream target of phosphatidylinositol-3-OH kinase [PI(3)K] signaling. We show that Drosophila 4E-BP...
Kozlov, Guennadi, De Crescenzo, Gregory, Lim, Nadia S, Siddiqui, Nadeem, Fantus, Daniel, Kahvejian, Avak, ...
The C-terminal domain of poly(A)-binding protein (PABC) is a peptide-binding domain found in poly(A)-binding proteins (PABPs) and a HECT (homologous to E6-AP C-terminus) family E3 ubiquitin ligase....
The protein kinase PKR: a molecular clock that sequentially activates survival and death programs
Donzé, Olivier, Deng, Jing, Curran, Joseph, Sladek, Robert, Picard, Didier, Sonenberg, Nahum
Cell death and survival play a key role in the immune system as well as during development. The control mechanisms that balance cell survival against cell death are not well understood. Here we...
Svitkin, Yuri V., Herdy, Barbara, Costa-Mattioli, Mauro, Gingras, Anne-Claude, Raught, Brian, Sonenberg, Nahum
Translation of m7G-capped cellular mRNAs is initiated by recruitment of ribosomes to the 5′ end of mRNAs via eukaryotic translation initiation factor 4F (eIF4F), a heterotrimeric complex comprised...
Akt-Mediated YB-1 Phosphorylation Activates Translation of Silent mRNA Species
Evdokimova, Valentina, Ruzanov, Peter, Anglesio, Michael S., Sorokin, Alexey V., Ovchinnikov, Lev P., Buckley, Jonathan, ...
YB-1 is a broad-specificity RNA-binding protein that is involved in regulation of mRNA transcription, splicing, translation, and stability. In both germinal and somatic cells, YB-1 and related...
ZUBEREK, JOANNA, WYSLOUCH-CIESZYNSKA, ALEKSANDRA, NIEDZWIECKA, ANNA, DADLEZ, MICHAL, STEPINSKI, JANUSZ, AUGUSTYNIAK, WOJCIECH, ...
Phosphorylation of the eukaryotic initiation factor eIF4E in response to mitogenic stimuli and cytokines is implicated in the regulation of the initiation step of translation. It still remains...
OGURO, AKIHIRO, OHTSU, TAKASHI, SVITKIN, YURI V., SONENBERG, NAHUM, NAKAMURA, YOSHIKAZU
The mammalian translation initiation factor 4A (eIF4A) is a prototype member of the DEAD-box RNA helicase family that couples ATPase activity to RNA binding and unwinding. In the crystal form, eIF4A...
MOCHIZUKI, KIYOTAKA, OGURO, AKIHIRO, OHTSU, TAKASHI, SONENBERG, NAHUM, NAKAMURA, YOSHIKAZU
The eukaryotic translation initiation factor 4F (eIF4F) consists of three polypeptides (eIF4A, eIF4G, and eIF4E) and is responsible for recruiting ribosomes to mRNA. eIF4E recognizes the mRNA...
Berlanga, Juan José, Baass, Alexis, Sonenberg, Nahum
The 5′ cap and 3′ poly(A) tail of eukaryotic mRNAs act synergistically to enhance translation. This synergy is mediated via interactions between eIF4G (a component of the eIF4F cap binding...
Le Bacquer, Olivier, Petroulakis, Emmanuel, Paglialunga, Sabina, Poulin, Francis, Richard, Denis, Cianflone, Katherine, ...
The most common pathology associated with obesity is insulin resistance, which results in the onset of type 2 diabetes mellitus. Several studies have implicated the mammalian target of rapamycin...
Epigenetic Activation of a Subset of mRNAs by eIF4E Explains Its Effects on Cell Proliferation
Mamane, Yaël, Petroulakis, Emmanuel, Martineau, Yvan, Sato, Taka-Aki, Larsson, Ola, Rajasekhar, Vinagolu K., ...
Koritzinsky, Marianne, Magagnin, Michaël G, Van Den Beucken, Twan, Seigneuric, Renaud, Savelkouls, Kim, Dostie, Josée, ...
Hypoxia has recently been shown to activate the endoplasmic reticulum kinase PERK, leading to phosphorylation of eIF2α and inhibition of mRNA translation initiation. Using a quantitative assay, we...
Miyakawa, Shin, Oguro, Akihiro, Ohtsu, Takashi, Imataka, Hiroaki, Sonenberg, Nahum, Nakamura, Yoshikazu
Eukaryotic translation initiation factor 4G (eIF4G) plays a crucial multimodulatory role in mRNA translation and decay by interacting with other translation factors and mRNA-associated proteins. In...
Antiviral effect of the mammalian translation initiation factor 2α kinase GCN2 against RNA viruses
Berlanga, Juan J, Ventoso, Iván, Harding, Heather P, Deng, Jing, Ron, David, Sonenberg, Nahum, ...
In mammals, four different protein kinases, heme-regulated inhibitor, double-stranded RNA-dependent protein kinase (PKR), general control non-derepressible-2 (GCN2) and PKR-like endoplasmic reticulum...
Poly(A) binding protein (PABP) homeostasis is mediated by the stability of its inhibitor, Paip2
Yoshida, Madoka, Yoshida, Kaori, Kozlov, Guennadi, Lim, Nadia S, De Crescenzo, Gregory, Pang, Zhiyu, ...
The poly(A)-binding protein (PABP) is a unique translation initiation factor in that it binds to the mRNA 3′ poly(A) tail and stimulates recruitment of the ribosome to the mRNA at the 5′ end....
The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity
Shahbazian, David, Roux, Philippe P, Mieulet, Virginie, Cohen, Michael S, Raught, Brian, Taunton, Jack, ...
The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. In response to insulin, eIF4B is phosphorylated on Ser422 by S6K in...
Weak binding affinity of human 4EHP for mRNA cap analogs
Zuberek, Joanna, Kubacka, Dorota, Jablonowska, Agnieszka, Jemielity, Jacek, Stepinski, Janusz, Sonenberg, Nahum, ...
Ribosome recruitment to the majority of eukaryotic mRNAs is facilitated by the interaction of the cap binding protein, eIF4E, with the mRNA 5′ cap structure. eIF4E stimulates translation through...
Burgui, Idoia, Yángüez, Emilio, Sonenberg, Nahum, Nieto, Amelia
Influenza virus mRNAs bear a short capped oligonucleotide sequence at their 5′ ends derived from the host cell pre-mRNAs by a “cap-snatching” mechanism, followed immediately by a common viral...
A role for the eIF4E-binding protein 4E-T in P-body formation and mRNA decay
Ferraiuolo, Maria A., Basak, Sanjukta, Dostie, Josee, Murray, Elizabeth L., Schoenberg, Daniel R., Sonenberg, Nahum
4E-transporter (4E-T) is one of several proteins that bind the mRNA 5′cap-binding protein, eukaryotic initiation factor 4E (eIF4E), through a conserved binding motif. We previously showed that 4E-T...
Nuclear Eukaryotic Initiation Factor 4e (Eif4e) Colocalizes with Splicing Factors in Speckles
Dostie, Josée, Lejbkowicz, Flavio, Sonenberg, Nahum
The eukaryotic initiation factor 4E (eIF4E) plays a pivotal role in the control of protein synthesis. eIF4E binds to the mRNA 5′ cap structure, m7GpppN (where N is any nucleotide) and promotes...
Svitkin, Yuri V., Costa-Mattioli, Mauro, Herdy, Barbara, Perreault, Sandra, Sonenberg, Nahum
Picornavirus infectivity is dependent on the RNA poly(A) tail, which binds the poly(A) binding protein (PABP). PABP was reported to stimulate viral translation and RNA synthesis. Here, we studied...
Reconstitution reveals the functional core of mammalian eIF3
Masutani, Mamiko, Sonenberg, Nahum, Yokoyama, Shigeyuki, Imataka, Hiroaki
Eukaryotic translation initiation factor (eIF)3 is the largest eIF (∼650 kDa), consisting of 10–13 different polypeptide subunits in mammalian cells. To understand the role of each subunit, we...
Oliere, Stephanie, Arguello, Meztli, Mesplede, Thibault, Tumilasci, Vanessa, Nakhaei, Peyman, Stojdl, David, ...
Vesicular stomatitis virus (VSV) is a candidate oncolytic virus that replicates and induces cell death in cancer cells while sparing normal cells. Although defects in the interferon antiviral...
Lee, Chung-Han, Inoki, Ken, Karbowniczek, Magdalena, Petroulakis, Emmanuel, Sonenberg, Nahum, Henske, Elizabeth Petri, ...
Miscoordination of growth and proliferation with the cellular stress response can lead to tumorigenesis. Mammalian target of rapamycin (mTOR), a central cell growth controller, is highly activated in...
The Dynamics of Mammalian P Body Transport, Assembly, and Disassembly In Vivo
Aizer, Adva, Brody, Yehuda, Ler, Lian Wee, Sonenberg, Nahum, Singer, Robert H., Shav-Tal, Yaron
Exported mRNAs are targeted for translation or can undergo degradation by several decay mechanisms. The 5′→3′ degradation machinery localizes to cytoplasmic P bodies (PBs). We followed the...
Control of eIF4E cellular localization by eIF4E-binding proteins, 4E-BPs
Rong, Liwei, Livingstone, Mark, Sukarieh, Rami, Petroulakis, Emmanuel, Gingras, Anne-Claude, Crosby, Katherine, ...
Eukaryotic initiation factor (eIF) 4E, the mRNA 5′-cap-binding protein, mediates the association of eIF4F with the mRNA 5′-cap structure to stimulate cap-dependent translation initiation in the...
Role of 3′UTRs in the Translation of mRNAs Regulated by Oncogenic eIF4E—A Computational Inference
Santhanam, Arti N., Bindewald, Eckart, Rajasekhar, Vinagolu K., Larsson, Ola, Sonenberg, Nahum, Colburn, Nancy H., ...
Eukaryotic cap-dependent mRNA translation is mediated by the initiation factor eIF4E, which binds mRNAs and stimulates efficient translation initiation. eIF4E is often overexpressed in human cancers....
Santhanam, Arti N., Bindewald, Eckart, Rajasekhar, Vinagolu K., Larsson, Ola, Sonenberg, Nahum, Colburn, Nancy H., ...
Martineau, Yvan, Derry, Mélanie C., Wang, Xiaoshan, Yanagiya, Akiko, Berlanga, Juan José, Shyu, Ann-Bin, ...
Poly(A)-binding protein (PABP) stimulates translation initiation by binding simultaneously to the mRNA poly(A) tail and eukaryotic translation initiation factor 4G (eIF4G). PABP activity is regulated...
Yanagiya, Akiko, Svitkin, Yuri V., Shibata, Shoichiro, Mikami, Satoshi, Imataka, Hiroaki, Sonenberg, Nahum
Eukaryotic mRNAs possess a 5′-terminal cap structure (cap), m7GpppN, which facilitates ribosome binding. The cap is bound by eukaryotic translation initiation factor 4F (eIF4F), which is composed...