Nica Borgese

The quest for a better resolution of protein-translocation processes. Conference on the Control, Co-ordination and Regulation of Protein Targeting and Translocation (2009)

Borgese, Nica, Driessen, Arnold J.M., Rapaport, Doron, Robinson, Colin

Targeting of a Tail-anchored Protein to Endoplasmic Reticulum and Mitochondrial Outer Membrane by Independent but Competing Pathways

Borgese, Nica, Gazzoni, Ilaria, Barberi, Massimo, Colombo, Sara, Pedrazzini, Emanuela

Many mitochondrial outer membrane (MOM) proteins have a transmembrane domain near the C terminus and an N-terminal cytosolic moiety. It is not clear how these tail-anchored (TA) proteins...

KDEL and KKXX Retrieval Signals Appended to the Same Reporter Protein Determine Different Trafficking between Endoplasmic Reticulum, Intermediate Compartment, and Golgi Complex

Stornaiuolo, Mariano, Lotti, Lavinia V., Borgese, Nica, Torrisi, Maria-Rosaria, Mottola, Giovanna, Martire, Gianluca, ...

Many endoplasmic reticulum (ER) proteins maintain their residence by dynamic retrieval from downstream compartments of the secretory pathway. In previous work we compared the retrieval process...

Transmembrane topogenesis of a tail-anchored protein is modulated by membrane lipid composition

Brambillasca, Silvia, Yabal, Monica, Soffientini, Paolo, Stefanovic, Sandra, Makarow, Marja, Hegde, Ramanujan S, ...

A large class of proteins with cytosolic functional domains is anchored to selected intracellular membranes by a single hydrophobic segment close to the C-terminus. Although such tail-anchored (TA)...

Two tail-anchored protein variants, differing in transmembrane domain length and intracellular sorting, interact differently with lipids

Ceppi, Paolo, Colombo, Sara, Francolini, Maura, Raimondo, Francesca, Borgese, Nica, Masserini, Massimo

C-tail-anchored (TA) proteins often require a transmembrane domain of moderate hydrophobicity to maintain their endoplasmic reticulum residence, but the suggested role of protein-lipid interactions...

Targeting of a Tail-anchored Protein to Endoplasmic Reticulum and Mitochondrial Outer Membrane by Independent but Competing Pathways

Borgese, Nica, Gazzoni, Ilaria, Barberi, Massimo, Colombo, Sara, Pedrazzini, Emanuela

Many mitochondrial outer membrane (MOM) proteins have a transmembrane domain near the C terminus and an N-terminal cytosolic moiety. It is not clear how these tail-anchored (TA) proteins...

KDEL and KKXX Retrieval Signals Appended to the Same Reporter Protein Determine Different Trafficking between Endoplasmic Reticulum, Intermediate Compartment, and Golgi Complex

Stornaiuolo, Mariano, Lotti, Lavinia V., Borgese, Nica, Torrisi, Maria-Rosaria, Mottola, Giovanna, Martire, Gianluca, ...

Many endoplasmic reticulum (ER) proteins maintain their residence by dynamic retrieval from downstream compartments of the secretory pathway. In previous work we compared the retrieval process...

Transmembrane topogenesis of a tail-anchored protein is modulated by membrane lipid composition

Brambillasca, Silvia, Yabal, Monica, Soffientini, Paolo, Stefanovic, Sandra, Makarow, Marja, Hegde, Ramanujan S, ...

A large class of proteins with cytosolic functional domains is anchored to selected intracellular membranes by a single hydrophobic segment close to the C-terminus. Although such tail-anchored (TA)...

Two tail-anchored protein variants, differing in transmembrane domain length and intracellular sorting, interact differently with lipids

Ceppi, Paolo, Colombo, Sara, Francolini, Maura, Raimondo, Francesca, Borgese, Nica, Masserini, Massimo

C-tail-anchored (TA) proteins often require a transmembrane domain of moderate hydrophobicity to maintain their endoplasmic reticulum residence, but the suggested role of protein-lipid interactions...

Unassisted translocation of large polypeptide domains across phospholipid bilayers

Brambillasca, Silvia, Yabal, Monica, Makarow, Marja, Borgese, Nica

Although transmembrane proteins generally require membrane-embedded machinery for integration, a few can insert spontaneously into liposomes. Previously, we established that the tail-anchored (TA)...

N-myristoylation determines dual targeting of mammalian NADH-cytochrome b(5) reductase to ER and mitochondrial outer membranes by a mechanism of kinetic partitioning

Colombo, Sara, Longhi, Renato, Alcaro, Stefano, Ortuso, Francesco, Sprocati, Teresa, Flora, Adriano, ...

Mammalian NADH-cytochrome b(5) reductase (b5R) is an N-myristoylated protein that is dually targeted to ER and mitochondrial outer membranes. The N-linked myristate is not required for anchorage to...

The tale of tail-anchored proteins: coming from the cytosol and looking for a membrane

Borgese, Nica, Colombo, Sara, Pedrazzini, Emanuela

A group of integral membrane proteins, known as C-tail anchored, is defined by the presence of a cytosolic NH2-terminal domain that is anchored to the phospholipid bilayer by a single segment of...

Formation of stacked ER cisternae by low affinity protein interactions

Snapp, Erik L., Hegde, Ramanujan S., Francolini, Maura, Lombardo, Francesca, Colombo, Sara, Pedrazzini, Emanuela, ...

The endoplasmic reticulum (ER) can transform from a network of branching tubules into stacked membrane arrays (termed organized smooth ER [OSER]) in response to elevated levels of specific resident...

Mechanism of Residence of Cytochrome B(5), a Tail-Anchored Protein, in the Endoplasmic Reticulum

Pedrazzini, Emanuela, Villa, Antonello, Longhi, Renato, Bulbarelli, Alessandra, Borgese, Nica

Endoplasmic reticulum (ER) proteins maintain their residency by static retention, dynamic retrieval, or a combination of the two. Tail-anchored proteins that contain a cytosolic domain associated...

Transmembrane domain–dependent partitioning of membrane proteins within the endoplasmic reticulum

Ronchi, Paolo, Colombo, Sara, Francolini, Maura, Borgese, Nica

The length and hydrophobicity of the transmembrane domain (TMD) play an important role in the sorting of membrane proteins within the secretory pathway; however, the relative contributions of...