DOI 10.1007/s00425-005-1563-z PROGRESS REPORT (2008)
Jagdish Tewari, Æ Charles, T. Hunter, Xuemei Li, Æ Anthony, ...
Artificial neural networks Plant cell walls are composed of independent but interacting networks of carbohydrates, proteins, and aromatic substances. Interacting with this complex matrix are several...
Dunn, Erin K., Shoue, Douglas A., Huang, Xuemei, Kline, Raymond E., MacKay, Alex L., Carpita, Nicholas C., ...
Although the Dasycladalean alga Acetabularia acetabulum has long been known to contain mannan-rich walls, it is not known to what extent wall composition varies as a function of the elaborate...
Las B-galactosidasas y la dinámica de la pared celular (2006)
Iris Pérez Almeida, Nicholas C. Carpita
Las B-galactosidasas y la dinámica de la pared celular
Changes in pectins of the Xylopodium of Ocimum nudicaule from dormancy to sprouting (2006)
Braga,Márcia Regina, Carpita,Nicholas C., Dietrich,Sonia M. C.
The thickened underground organ of Ocimum nudicaule is a tuber-like structure (xylopodium) that is dormant in winter and sprouts at the beginning of the spring. Changes in content of cell wall...
Dunn, Erin K., Shoue, Douglas A., Huang, Xuemei, Kline, Raymond E., MacKay, Alex L., Carpita, Nicholas C., ...
Although the Dasycladalean alga Acetabularia acetabulum has long been known to contain mannan-rich walls, it is not known to what extent wall composition varies as a function of the elaborate...
Schindelman, Gary, Morikami, Atsushi, Jung, Jee, Baskin, Tobias I., Carpita, Nicholas C., Derbyshire, Paul, ...
Typescript (photocopy)
Cell Wall and Membrane-Associated Exo-β-d-Glucanases from Developing Maize Seedlings1
Kim, Jong-Bum, Olek, Anna T., Carpita, Nicholas C.
A β-d-glucan exohydrolase was purified from the cell walls of developing maize (Zea mays L.) shoots. The cell wall enzyme preferentially hydrolyzes the non-reducing terminal glucosyl residue from...
Buckeridge, Marcos S., Vergara, Claudia E., Carpita, Nicholas C.
We examined the mechanism of synthesis in vitro of (1→3),(1→4)β-d-glucan (β-glucan), a growth-specific cell wall polysaccharide found in grasses and cereals. β-Glucan is composed primarily of...
Vanzin, Gary F., Madson, Michael, Carpita, Nicholas C., Raikhel, Natasha V., Keegstra, Kenneth, Reiter, Wolf-Dieter
Cell walls of the Arabidopsis mutant mur2 contain less than 2% of the wild-type amount of fucosylated xyloglucan because of a point mutation in the fucosyltransferase AtFUT1. The mur2 mutation...
Cell Wall Architecture of the Elongating Maize Coleoptile1
Carpita, Nicholas C., Defernez, Marianne, Findlay, Kim, Wells, Brian, Shoue, Douglas A., Catchpole, Gareth, ...
The primary walls of grasses are composed of cellulose microfibrils, glucuronoarabinoxylans (GAXs), and mixed-linkage β-glucans, together with smaller amounts of xyloglucans, glucomannans, pectins,...
MYB61 Is Required for Mucilage Deposition and Extrusion in the Arabidopsis Seed Coat
Penfield, Steven, Meissner, Ruth C., Shoue, Douglas A., Carpita, Nicholas C., Bevan, Michael W.
We have undertaken a systematic reverse genetic approach to understand R2R3-MYB gene function in Arabidopsis. Here, we report the functional characterization of MYB61 based on the phenotype of three...
Madson, Michael, Dunand, Christophe, Li, Xuemei, Verma, Rajeev, Vanzin, Gary F., Caplan, Jeffrey, ...
Xyloglucans are the principal glycans that interlace cellulose microfibrils in most flowering plants. The mur3 mutant of Arabidopsis contains a severely altered structure of this polysaccharide...
Zhu, Yanmin, Nam, Jaesung, Carpita, Nicholas C., Matthysse, Ann G., Gelvin, Stanton B.
Agrobacterium-mediated plant genetic transformation involves a complex interaction between the bacterium and the host plant. Relatively little is known about the role plant genes and proteins play in...
Peña, María J., Ryden, Peter, Madson, Michael, Smith, Andrew C., Carpita, Nicholas C.
In land plants, xyloglucans (XyGs) tether cellulose microfibrils into a strong but extensible cell wall. The MUR2 and MUR3 genes of Arabidopsis encode XyG-specific fucosyl and galactosyl...
Topology of the Maize Mixed Linkage (1→3),(1→4)-β-D-Glucan Synthase at the Golgi Membrane1
Urbanowicz, Breeanna R., Rayon, Catherine, Carpita, Nicholas C.
Mixed-linkage (1→3),(1→4)-β-d-glucan is a plant cell wall polysaccharide composed of cellotriosyl and cellotetraosyl units, with decreasingly smaller amounts of cellopentosyl, cellohexosyl, and...
Carpita, Nicholas C., Brown, Ronald A., Weller, Kathleen M.
According to the acid-growth hypothesis, auxin-induced secretion of hydrogen ions activate “wall loosening” enzymes that change the rheological properties of the cell wall. The wall loosening...
Protection of Cellulose Synthesis in Detached Cotton Fibers by Polyethylene Glycol 1
Carpita, Nicholas C., Delmer, Deborah P.
Detachment of the cotton fiber cell from the ovule results in loss of over 90% of the in vivo capacity for synthesis of [14C]cellulose from [14C]glucose. However, over 50% of the capacity for...
Peña, María J., Carpita, Nicholas C.
Growth and maturation of the edible cortical cells of apples (Malus domestica Borkh) are accompanied by a selective loss of pectin-associated (1→4)-β-d-galactan from the cell walls, whereas a...
Effects of 35 C Heat Treatments on Photosensitive Grand Rapids Lettuce Seed Germination 1
Carpita, Nicholas C., Nabors, Murray W.
Grand Rapids lettuce (Lactuca sativa L.) seeds were given 35 C heat treatments to increase photodormancy in a subsequent 20 C dark period. Short heat treatments (1-5 hours) induced a significant...
Maltby, David, Carpita, Nicholas C., Montezinos, David, Kulow, Carl, Delmer, Deborah P.
Evidence is presented for the existence of a noncellulosic β-1,3-glucan in cotton fibers. The glucan can be isolated as distinct fractions of varying solubility. When fibers are homogenized...
Chemical Structure of the Cell Walls of Dwarf Maize and Changes Mediated by Gibberellin 1
Carpita, Nicholas C., Kanabus, Jan
Dwarf maize (Zea mays L.), a mutant deficient in gibberellin synthesis, provides an excellent model to study the influence of gibberellin on biochemical processes related to plant development....
Carbon Assimilation in Carrot Cells in Liquid Culture 1
Kanabus, Jan, Bressan, Ray A., Carpita, Nicholas C.
Assimilation of carbohydrates by carrot (Daucus carota L. cv Danvers) cells in liquid culture was studied to delineate the major metabolic pathways used in transformation of external carbohydrates to...
Iraki, Naim M., Bressan, Ray A., Hasegawa, P. M., Carpita, Nicholas C.
Cells of tobacco (Nicotiana tabacum L.) adapted to grow in severe osmotic stress of 428 millimolar NaCl (−23 bar) or 30% polyethylene glycol 8000 (−28 bar) exhibit a drastically altered growth...
Iraki, Naim M., Singh, Narendra, Bressan, Ray A., Carpita, Nicholas C.
The relative mass of the cell walls of tobacco (Nicotiana tabacum L.) cells adapted to grow in medium containing 30% polyethylene glycol 8000 or 428 millimolar NaCl was reduced to about 50% of that...
Iraki, Naim M., Bressan, Ray A., Carpita, Nicholas C.
The chemical composition of extracellular polymers released by cells of tobacco (Nicotiana tabacum L. cv W38) adapted to a medium containing 30% polyethylene glycol 8000 (−28 bar) or 428 millimolar...
Cell Wall Development in Maize Coleoptiles 1
The physical bases for enhancement of growth rates induced by auxin involve changes in cell wall structure. Changes in the chemical composition of the primary walls during maize (Zea mays L. cv WF9...
Hemicellulosic Polymers of Cell Walls of Zea Coleoptiles 1
Hemicellulosic polymers comprised about 43% of the primary walls of Zea mays L. cv WF9 × Bear 38 coleoptiles; these polymers were separated by an alkali-gradient into three major fractions. Fraction...
Solutes Contributing to Osmotic Adjustment in Cultured Plant Cells Adapted to Water Stress 1
Handa, Sangita, Bressan, Ray A., Handa, Avtar K., Carpita, Nicholas C., Hasegawa, Paul M.
Osmotic adjustment was studied in cultured cells of tomato (Lycopersicon esculentum Mill cv VFNT-Cherry) adapted to different levels of external water potential ranging from −4 bar to −28 bar....
Hemicelluloses of Cell Walls of a Proso Millet Cell Suspension Culture 1
Carpita, Nicholas C., Mulligan, Jenny A., Heyser, James W.
Cell wall composition of a stable suspension of proso millet (Panicum miliaceum L. cv Abarr) cells is similar to those of tissues and cell suspensions of other graminaceous species. Extraction of...
Tensile Strength of Cell Walls of Living Cells 1
A gas decompression technique was used to determine the breaking strength of cell walls of single cells. Breaking strengths of the bacterium Salmonella typhimurium and the unicellular green alga...
Incorporation of Proline and Aromatic Amino Acids into Cell Walls of Maize Coleoptiles 1
Sections excised from maize coleoptiles incorporated radioactivity from proline, tyrosine, and phenylalanine into structural components of the cell wall. Only about 2% of radioactivity from proline...
Kim, Jong-Bum, Carpita, Nicholas C.
Cell walls of grasses have two major polysaccharides that contain uronic acids, the hemicellulosic glucuronoarabinoxylans and the galactosyluronic acid-rich pectins. A technique whereby esterified...
Fructosyl Transfer between 1-Kestose and Sucrose in Wheat Leaves 1
Kanabus, Jan, Gibeaut, David M., Carpita, Nicholas C., Housley, Thomas L.
The labeling pattern of the sugar moieties of 1-kestose after in vivo pulse labeling with 14CO2 was not the same as that after in vitro labeling with 14C-sucrose. The two fructosyl residues of...
Gibeaut, David M., Carpita, Nicholas C.
Cells of proso millet (Panicum miliaceum L. cv Abarr) in liquid culture and leaves of maize seedlings (Zea mays L. cv LH51 × LH1131) readily incorporated d-[U-14C]glucose and l-[U-14C]arabinose into...
Schindelman, Gary, Morikami, Atsushi, Jung, Jee, Baskin, Tobias I., Carpita, Nicholas C., Derbyshire, Paul, ...
To control organ shape, plant cells expand differentially. The organization of the cellulose microfibrils in the cell wall is a key determinant of differential expansion. Mutations in the COBRA (COB)...
Cell Wall and Membrane-Associated Exo-β-d-Glucanases from Developing Maize Seedlings1
Kim, Jong-Bum, Olek, Anna T., Carpita, Nicholas C.
A β-d-glucan exohydrolase was purified from the cell walls of developing maize (Zea mays L.) shoots. The cell wall enzyme preferentially hydrolyzes the non-reducing terminal glucosyl residue from...
Buckeridge, Marcos S., Vergara, Claudia E., Carpita, Nicholas C.
We examined the mechanism of synthesis in vitro of (1→3),(1→4)β-d-glucan (β-glucan), a growth-specific cell wall polysaccharide found in grasses and cereals. β-Glucan is composed primarily of...
Vanzin, Gary F., Madson, Michael, Carpita, Nicholas C., Raikhel, Natasha V., Keegstra, Kenneth, Reiter, Wolf-Dieter
Cell walls of the Arabidopsis mutant mur2 contain less than 2% of the wild-type amount of fucosylated xyloglucan because of a point mutation in the fucosyltransferase AtFUT1. The mur2 mutation...
Cell Wall Architecture of the Elongating Maize Coleoptile1
Carpita, Nicholas C., Defernez, Marianne, Findlay, Kim, Wells, Brian, Shoue, Douglas A., Catchpole, Gareth, ...
The primary walls of grasses are composed of cellulose microfibrils, glucuronoarabinoxylans (GAXs), and mixed-linkage β-glucans, together with smaller amounts of xyloglucans, glucomannans, pectins,...
MYB61 Is Required for Mucilage Deposition and Extrusion in the Arabidopsis Seed Coat
Penfield, Steven, Meissner, Ruth C., Shoue, Douglas A., Carpita, Nicholas C., Bevan, Michael W.
We have undertaken a systematic reverse genetic approach to understand R2R3-MYB gene function in Arabidopsis. Here, we report the functional characterization of MYB61 based on the phenotype of three...
Madson, Michael, Dunand, Christophe, Li, Xuemei, Verma, Rajeev, Vanzin, Gary F., Caplan, Jeffrey, ...
Xyloglucans are the principal glycans that interlace cellulose microfibrils in most flowering plants. The mur3 mutant of Arabidopsis contains a severely altered structure of this polysaccharide...
Zhu, Yanmin, Nam, Jaesung, Carpita, Nicholas C., Matthysse, Ann G., Gelvin, Stanton B.
Agrobacterium-mediated plant genetic transformation involves a complex interaction between the bacterium and the host plant. Relatively little is known about the role plant genes and proteins play in...
Schindelman, Gary, Morikami, Atsushi, Jung, Jee, Baskin, Tobias I., Carpita, Nicholas C., Derbyshire, Paul, ...
To control organ shape, plant cells expand differentially. The organization of the cellulose microfibrils in the cell wall is a key determinant of differential expansion. Mutations in the COBRA (COB)...
Peña, María J., Ryden, Peter, Madson, Michael, Smith, Andrew C., Carpita, Nicholas C.
In land plants, xyloglucans (XyGs) tether cellulose microfibrils into a strong but extensible cell wall. The MUR2 and MUR3 genes of Arabidopsis encode XyG-specific fucosyl and galactosyl...
Topology of the Maize Mixed Linkage (1→3),(1→4)-β-D-Glucan Synthase at the Golgi Membrane1
Urbanowicz, Breeanna R., Rayon, Catherine, Carpita, Nicholas C.
Mixed-linkage (1→3),(1→4)-β-d-glucan is a plant cell wall polysaccharide composed of cellotriosyl and cellotetraosyl units, with decreasingly smaller amounts of cellopentosyl, cellohexosyl, and...
Carpita, Nicholas C., Brown, Ronald A., Weller, Kathleen M.
According to the acid-growth hypothesis, auxin-induced secretion of hydrogen ions activate “wall loosening” enzymes that change the rheological properties of the cell wall. The wall loosening...
Protection of Cellulose Synthesis in Detached Cotton Fibers by Polyethylene Glycol 1
Carpita, Nicholas C., Delmer, Deborah P.
Detachment of the cotton fiber cell from the ovule results in loss of over 90% of the in vivo capacity for synthesis of [14C]cellulose from [14C]glucose. However, over 50% of the capacity for...
Peña, María J., Carpita, Nicholas C.
Growth and maturation of the edible cortical cells of apples (Malus domestica Borkh) are accompanied by a selective loss of pectin-associated (1→4)-β-d-galactan from the cell walls, whereas a...
Effects of 35 C Heat Treatments on Photosensitive Grand Rapids Lettuce Seed Germination 1
Carpita, Nicholas C., Nabors, Murray W.
Grand Rapids lettuce (Lactuca sativa L.) seeds were given 35 C heat treatments to increase photodormancy in a subsequent 20 C dark period. Short heat treatments (1-5 hours) induced a significant...
Maltby, David, Carpita, Nicholas C., Montezinos, David, Kulow, Carl, Delmer, Deborah P.
Evidence is presented for the existence of a noncellulosic β-1,3-glucan in cotton fibers. The glucan can be isolated as distinct fractions of varying solubility. When fibers are homogenized...
Chemical Structure of the Cell Walls of Dwarf Maize and Changes Mediated by Gibberellin 1
Carpita, Nicholas C., Kanabus, Jan
Dwarf maize (Zea mays L.), a mutant deficient in gibberellin synthesis, provides an excellent model to study the influence of gibberellin on biochemical processes related to plant development....
Carbon Assimilation in Carrot Cells in Liquid Culture 1
Kanabus, Jan, Bressan, Ray A., Carpita, Nicholas C.
Assimilation of carbohydrates by carrot (Daucus carota L. cv Danvers) cells in liquid culture was studied to delineate the major metabolic pathways used in transformation of external carbohydrates to...
Iraki, Naim M., Bressan, Ray A., Hasegawa, P. M., Carpita, Nicholas C.
Cells of tobacco (Nicotiana tabacum L.) adapted to grow in severe osmotic stress of 428 millimolar NaCl (−23 bar) or 30% polyethylene glycol 8000 (−28 bar) exhibit a drastically altered growth...
Iraki, Naim M., Singh, Narendra, Bressan, Ray A., Carpita, Nicholas C.
The relative mass of the cell walls of tobacco (Nicotiana tabacum L.) cells adapted to grow in medium containing 30% polyethylene glycol 8000 or 428 millimolar NaCl was reduced to about 50% of that...
Iraki, Naim M., Bressan, Ray A., Carpita, Nicholas C.
The chemical composition of extracellular polymers released by cells of tobacco (Nicotiana tabacum L. cv W38) adapted to a medium containing 30% polyethylene glycol 8000 (−28 bar) or 428 millimolar...
Cell Wall Development in Maize Coleoptiles 1
The physical bases for enhancement of growth rates induced by auxin involve changes in cell wall structure. Changes in the chemical composition of the primary walls during maize (Zea mays L. cv WF9...
Hemicellulosic Polymers of Cell Walls of Zea Coleoptiles 1
Hemicellulosic polymers comprised about 43% of the primary walls of Zea mays L. cv WF9 × Bear 38 coleoptiles; these polymers were separated by an alkali-gradient into three major fractions. Fraction...
Solutes Contributing to Osmotic Adjustment in Cultured Plant Cells Adapted to Water Stress 1
Handa, Sangita, Bressan, Ray A., Handa, Avtar K., Carpita, Nicholas C., Hasegawa, Paul M.
Osmotic adjustment was studied in cultured cells of tomato (Lycopersicon esculentum Mill cv VFNT-Cherry) adapted to different levels of external water potential ranging from −4 bar to −28 bar....
Hemicelluloses of Cell Walls of a Proso Millet Cell Suspension Culture 1
Carpita, Nicholas C., Mulligan, Jenny A., Heyser, James W.
Cell wall composition of a stable suspension of proso millet (Panicum miliaceum L. cv Abarr) cells is similar to those of tissues and cell suspensions of other graminaceous species. Extraction of...
Tensile Strength of Cell Walls of Living Cells 1
A gas decompression technique was used to determine the breaking strength of cell walls of single cells. Breaking strengths of the bacterium Salmonella typhimurium and the unicellular green alga...
Incorporation of Proline and Aromatic Amino Acids into Cell Walls of Maize Coleoptiles 1
Sections excised from maize coleoptiles incorporated radioactivity from proline, tyrosine, and phenylalanine into structural components of the cell wall. Only about 2% of radioactivity from proline...
Kim, Jong-Bum, Carpita, Nicholas C.
Cell walls of grasses have two major polysaccharides that contain uronic acids, the hemicellulosic glucuronoarabinoxylans and the galactosyluronic acid-rich pectins. A technique whereby esterified...
Fructosyl Transfer between 1-Kestose and Sucrose in Wheat Leaves 1
Kanabus, Jan, Gibeaut, David M., Carpita, Nicholas C., Housley, Thomas L.
The labeling pattern of the sugar moieties of 1-kestose after in vivo pulse labeling with 14CO2 was not the same as that after in vitro labeling with 14C-sucrose. The two fructosyl residues of...
Gibeaut, David M., Carpita, Nicholas C.
Cells of proso millet (Panicum miliaceum L. cv Abarr) in liquid culture and leaves of maize seedlings (Zea mays L. cv LH51 × LH1131) readily incorporated d-[U-14C]glucose and l-[U-14C]arabinose into...
Neural Network Analyses of Infrared Spectra for Classifying Cell Wall Architectures1[W][OA]
McCann, Maureen C., Defernez, Marianne, Urbanowicz, Breeanna R., Tewari, Jagdish C., Langewisch, Tiffany, Olek, Anna, ...
About 10% of plant genomes are devoted to cell wall biogenesis. Our goal is to establish methodologies that identify and classify cell wall phenotypes of mutants on a genome-wide scale. Toward this...
Dean, Gillian H., Zheng, Huanquan, Tewari, Jagdish, Huang, Jun, Young, Diana S., Hwang, Yeen Ting, ...
Seed coat development in Arabidopsis thaliana involves a complex pathway where cells of the outer integument differentiate into a highly specialized cell type after fertilization. One aspect of this...
Sindhu, Anoop, Langewisch, Tiffany, Olek, Anna, Multani, Dilbag S., McCann, Maureen C., Vermerris, Wilfred, ...
The maize (Zea mays) brittle stalk2 (bk2) is a recessive mutant, the aerial parts of which are easily broken. The bk2 phenotype is developmentally regulated and appears 4 weeks after planting, at...
Novel Rhamnogalacturonan I and Arabinoxylan Polysaccharides of Flax Seed Mucilage1[C][OA]
Naran, Radnaa, Chen, Guibing, Carpita, Nicholas C.
The viscous seed mucilage of flax (Linum usitatissimum) is a mixture of rhamnogalacturonan I and arabinoxylan with novel side group substitutions. The rhamnogalacturonan I has numerous single...
Held, Michael A., Penning, Bryan, Brandt, Amanda S., Kessans, Sarah A., Yong, Weidong, Scofield, Steven R., ...
Small-interfering RNAs (siRNAs) from natural cis-antisense pairs derived from the 3′-coding region of the barley (Hordeum vulgare) CesA6 cellulose synthase gene substantially increase in abundance...
Arsovski, Andrej A., Popma, Theodore M., Haughn, George W., Carpita, Nicholas C., McCann, Maureen C., Western, Tamara L.
Following pollination, the epidermal cells of the Arabidopsis (Arabidopsis thaliana) ovule undergo a complex differentiation process that includes the synthesis and polar secretion of pectinaceous...