Nico van Rooijen

Resident macrophages influence stem cell activity in the mammary gland (2009)

Gyorki, David E, Asselin-Labat, Marie-Liesse, Van Rooijen, Nico, Lindeman, Geoffrey J, Visvader, Jane E

Abstract Introduction Macrophages in the mammary gland are essential for morphogenesis of the ductal epithelial tree and have been implicated in promoting breast tumor metastasis. Although it is well...

Immune-to-brain signalling: the role of cerebral CD163-positive macrophages (2008)

Galea, Ian, Felton, Leigh M., Waters, Sara, Van Rooijen, Nico, Perry, V. Hugh, Newman, Tracey A.

Systemic inflammation induces cytokine synthesis within the central nervous system. This results in sickness behaviour and may exacerbate ongoing neuroinflammatory disease. The precise mechanisms...

Roles of PI3K and JAK pathways in viability of retinal ganglion cells after acute elevation of intraocular pressure in rats with different autoimmune backgrounds (2008)

Huang, Yao, Li, Zhiwei, Wang, Ningli, Van Rooijen, Nico, Cui, Qi

Abstract Background We recently showed that whereas inhibition of PI3K/akt or JAK/STAT pathway promoted retinal ganglion cell (RGC) survival after optic nerve (ON) injury in Fischer 344 (F344) rats,...

Blood flow-dependent arterial remodelling is facilitated by inflammation but directed by vascular tone (2008)

Matlung, Hanke L., Bonta, Peter, De Vries, Carlie J., Van Rooijen, Nico, VanBavel, Ed

Aims Altered blood flow affects vascular tone, attracts inflammatory cells, and leads to microvascular remodelling. We tested the hypothesis that inflammation facilitates the remodelling response,...

Anti-PlGF inhibits growth of VEGF(R)-inhibitor-resistant tumors without affecting healthy vessels (2007)

Zacchigna, Serena, Pattarini, Lucia, ...

Novel antiangiogenic strategies with complementary mechanisms are needed to maximize efficacy and minimize resistance to current angiogenesis inhibitors. We explored the therapeutic potential and...

Phagocyte-derived catecholamines enhance acute inflammatory injury (2007)

Flierl, Michael A., Rittirsch, Daniel, Nadeau, Brian A., Chen, Anthony J., Sarma, J. Vidya, Zetoune, Firas S., ...

It is becoming increasingly clear that the autonomic nervous system and the immune system demonstrate cross-talk during inflammation by means of sympathetic and parasympathetic pathways(1,2). We...

An antigen-specific pathway for CD8 T cells across the blood-brain barrier (2007)

Galea, Ian, Bernardes-Silva, Martine, Forse, Penny A., Van Rooijen, Nico, Liblau, Roland S., Perry, V. Hugh

CD8 T cells are nature's foremost defense in encephalitis and brain tumors. Antigen-specific CD8 T cells need to enter the brain to exert their beneficial effects. On the other hand, traffic of CD8 T...

Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis. (2007)

Arnold, Ludovic, Henry, Adeline, Poron, Françoise, Baba-Amer, Yasmine, Van Rooijen, Nico, Plonquet, Anne, ...

Macrophages (MPs) are important for skeletal muscle regeneration in vivo and may exert beneficial effects on myogenic cell growth through mitogenic and antiapoptotic activities in vitro. However, MPs...

Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis. (2007)

Arnold, Ludovic, Henry, Adeline, Poron, Françoise, Baba-Amer, Yasmine, Van Rooijen, Nico, Plonquet, Anne, ...

Macrophages (MPs) are important for skeletal muscle regeneration in vivo and may exert beneficial effects on myogenic cell growth through mitogenic and antiapoptotic activities in vitro. However, MPs...

Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis. (2007)

Arnold, Ludovic, Henry, Adeline, Poron, Françoise, Baba-Amer, Yasmine, Van Rooijen, Nico, Plonquet, Anne, ...

Macrophages (MPs) are important for skeletal muscle regeneration in vivo and may exert beneficial effects on myogenic cell growth through mitogenic and antiapoptotic activities in vitro. However, MPs...

Enhanced early vascular permeability in gelatinase B (MMP-9)-deficient mice: putative contribution of COX-1-derived PGE2 of macrophage origin (2006)

Scislowska-Czarnecka, Anna, Chadzinska, Magdalena, Plytycz, Barbara, Van Rooijen, Nico, ...

Increased vascular permeability leading to vascular leakage is a central feature of all inflammatory reactions and is critical for the formation of an inflammatory exudate. The leakage occurs because...

Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis (2006)

Anderson, Per, Durbeej, Madeleine, Van Rooijen, Nico, Ivars, Fredrik, ...

The endothelial cell monolayer of cerebral vessels and its basement membrane (BM) are ensheathed by the astrocyte endfeet, the leptomeningeal cells, and their associated parenchymal BM, all of which...

Blood-derived dendritic cells in an acute brain injury (2005)

Newman, Tracey A., Galea, Ian, Van Rooijen, Nico, Perry, V. Hugh

Dendritic cells (DC), while absent from the normal CNS parenchyma, have been demonstrated in delayed type hypersensitivity (DTH), experimental allergic encephalomyelitis (EAE), and some MS lesions....

Silica particles enhance peripheral thrombosis: key role of lung macrophage-neutrophil cross-talk (2005)

Van Rooijen, Nico

RATIONALE: Inflammation and thrombosis are related via interactions between leukocytes, platelets, the vasculature, and the coagulation system. However, the mechanisms behind these interactions...

Mannose receptor expression specifically reveals perivascular macrophages in normal, injured, and diseased mouse brain (2005)

Galea, Ian, Palin, Karine, Newman, Tracey A., Van Rooijen, Nico, Perry, V. Hugh, Boche, Delphine

Perivascular macrophages are believed to have a significant role in inflammation in the central nervous system (CNS). They express a number of different receptors that point toward functions in both...

Differential requirements for CTL generation by novel immunostimulants: APC tropism, use of the TAP-independent processing pathway, and dependency on CD80/CD86 costimulation (2003)

Sheikh, Nadeem A., Attard, George S., Van Rooijen, Nico, Rajananthanan, Palasingam, Hariharan, Kandasamy, Yang, Ya-Wum, ...

A major drawback of subunit vaccines is their inability to generate cytolytic T lymphocytes (CTL), a deficit attributed to segregation of the class I and class II antigen-processing pathways. We...

Therapeutic factor VIII levels and negligible toxicity in mouse and dog models of hemophilia A following gene therapy with high-capacity adenoviral vectors (2003)

Schiedner, Gudrun, Brown, Brian, Johnston, Marion, ...

High-capacity adenoviral (HC-Ad) vectors expressing B-domain-deleted human or canine factor VIII from different liver-specific promoters were evaluated for gene therapy of hemophilia A. Intravenous...

Roles of Macrophages and Neutrophils in the Early Host Response to Bacillus anthracis Spores in a Mouse Model of Infection (1998)

Cote, Christopher K., Van Rooijen, Nico, Welkos, Susan L.

The development of new approaches to combat anthrax requires that the pathogenesis and host response to Bacillus anthracis spores be better understood. We investigated the roles that macrophages and...

Febrile-Range Temperature Modifies Early Systemic Tumor Necrosis Factor Alpha Expression in Mice Challenged with Bacterial Endotoxin

Jiang, Qingqi, DeTolla, Louis, Van Rooijen, Nico, Singh, Ishwar S., Fitzgerald, Bridget, Lipsky, Michael M., ...

Fever improves survival in acute infections, but the effects of increased core temperature on host defenses are poorly understood. Tumor necrosis factor alpha (TNF-α) is an early activator of host...

Improved Innate Immunity of Endotoxin-Tolerant Mice Increases Resistance to Salmonella enterica Serovar Typhimurium Infection despite Attenuated Cytokine Response

Lehner, Martin D., Ittner, Josepha, Bundschuh, Daniela S., Van Rooijen, Nico, Wendel, Albrecht, Hartung, Thomas

During infection with gram-negative bacteria, exposure of immune cells to lipopolysaccharide (LPS) from the bacterial cell membrane induces a rapid cytokine response which is essential for the...

Replication of Murine Cytomegalovirus in Differentiated Macrophages as a Determinant of Viral Pathogenesis

Hanson, Laura K., Slater, Jacquelyn S., Karabekian, Zaruhi, Virgin, Herbert W., Biron, Christine A., Ruzek, Melanie C., ...

Blood monocytes or tissue macrophages play a pivotal role in the pathogenesis of murine cytomegalovirus (MCMV) infection, providing functions beneficial to both the virus and the host. In vitro and...

Depletion of Blood-Borne Macrophages Does Not Reduce Demyelination in Mice Infected with a Neurotropic Coronavirus

Xue, Shurong, Sun, Ning, Van Rooijen, Nico, Perlman, Stanley

Mice infected with the neurotropic coronavirus mouse hepatitis virus strain JHM (MHV-JHM) develop a chronic demyelinating disease with symptoms of hindlimb paralysis. Histological examination of the...

Chemokine Receptor CCR2 Is Not Essential for the Development of Experimental Cerebral Malaria

Belnoue, Elodie, Costa, Fabio T. M., Vigário, Ana M., Voza, Tatiana, Gonnet, Françoise, Landau, Irène, ...

Infection with Plasmodium berghei ANKA induces cerebral malaria in susceptible mice. Brain-sequestered CD8+ T cells are responsible for this pathology. We have evaluated the role of CCR2, a chemokine...

The trans-Sialidase from Trypanosoma cruzi Induces Thrombocytopenia during Acute Chagas' Disease by Reducing the Platelet Sialic Acid Contents

Tribulatti, María Virginia, Mucci, Juan, Van Rooijen, Nico, Leguizamón, María Susana, Campetella, Oscar

Strong thrombocytopenia is observed during acute infection with Trypanosoma cruzi, the parasitic protozoan agent of American trypanosomiasis or Chagas' disease. The parasite sheds trans-sialidase, an...

Glycolipid presentation to natural killer T cells differs in an organ-dependent fashion

Schmieg, John, Yang, Guangli, Franck, Richard W., Van Rooijen, Nico, Tsuji, Moriya

It has been shown that dendritic cells (DCs) are able to present glycolipids to natural killer (NK) T cells in vivo. However, the essential role of DCs, as well as the role of other cells in...

Inflammation-induced lymphangiogenesis in the cornea arises from CD11b-positive macrophages

Maruyama, Kazuichi, Ii, Masaaki, Cursiefen, Claus, Jackson, David G., Keino, Hiroshi, Tomita, Minoru, ...

In the inflamed cornea, there is a parallel outgrowth of blood and lymphatic vessels into the normally avascular cornea. We tested whether adaptive and/or innate immune cells were actively involved...

Combined Immunostimulation and Conditional Cytotoxic Gene Therapy Provide Long-term Survival in a Large Glioma Model

Ali, Sumia, King, Gwendalyn D., Curtin, James F., Candolfi, Marianela, Xiong, Weidong, Liu, Chunyan, ...

In spite of preclinical efficacy and recent randomized, controlled studies with adenoviral vectors expressing herpes simplex virus-1 thymidine kinase (HSV1-TK) showing statistically significant...

Pathogenicity of Influenza Viruses with Genes from the 1918 Pandemic Virus: Functional Roles of Alveolar Macrophages and Neutrophils in Limiting Virus Replication and Mortality in Mice

Tumpey, Terrence M., García-Sastre, Adolfo, Taubenberger, Jeffery K., Palese, Peter, Swayne, David E., Pantin-Jackwood, Mary J., ...

The Spanish influenza pandemic of 1918 to 1919 swept the globe and resulted in the deaths of at least 20 million people. The basis of the pulmonary damage and high lethality caused by the 1918 H1N1...

Roles of Macrophages and Neutrophils in the Early Host Response to Bacillus anthracis Spores in a Mouse Model of Infection

Cote, Christopher K., Van Rooijen, Nico, Welkos, Susan L.

The development of new approaches to combat anthrax requires that the pathogenesis and host response to Bacillus anthracis spores be better understood. We investigated the roles that macrophages and...

Activated macrophages are essential in a murine model for T cell–mediated chronic psoriasiform skin inflammation

Wang, Honglin, Peters, Thorsten, Kess, Daniel, Sindrilaru, Anca, Oreshkova, Tsvetelina, Van Rooijen, Nico, ...

The CD18 hypomorphic (CD18hypo) PL/J mouse model clinically resembling human psoriasis is characterized by reduced expression of the common chain of β2 integrins (CD11/CD18) to only 2–16% of WT...

Pathogenic role for skin macrophages in a mouse model of keratinocyte-induced psoriasis-like skin inflammation

Stratis, Athanasios, Pasparakis, Manolis, Rupec, Rudolf A., Markur, Doreen, Hartmann, Karin, Scharffetter-Kochanek, Karin, ...

Psoriasis is a common skin disease, the pathogenesis of which has not yet been resolved. In mice, epidermis-specific deletion of inhibitor of NF-κB (IκB) kinase 2 (IKK2) results in a skin phenotype...

Febrile-Range Temperature Modifies Early Systemic Tumor Necrosis Factor Alpha Expression in Mice Challenged with Bacterial Endotoxin

Jiang, Qingqi, DeTolla, Louis, Van Rooijen, Nico, Singh, Ishwar S., Fitzgerald, Bridget, Lipsky, Michael M., ...

Fever improves survival in acute infections, but the effects of increased core temperature on host defenses are poorly understood. Tumor necrosis factor alpha (TNF-α) is an early activator of host...

Improved Innate Immunity of Endotoxin-Tolerant Mice Increases Resistance to Salmonella enterica Serovar Typhimurium Infection despite Attenuated Cytokine Response

Lehner, Martin D., Ittner, Josepha, Bundschuh, Daniela S., Van Rooijen, Nico, Wendel, Albrecht, Hartung, Thomas

During infection with gram-negative bacteria, exposure of immune cells to lipopolysaccharide (LPS) from the bacterial cell membrane induces a rapid cytokine response which is essential for the...

Replication of Murine Cytomegalovirus in Differentiated Macrophages as a Determinant of Viral Pathogenesis

Hanson, Laura K., Slater, Jacquelyn S., Karabekian, Zaruhi, Virgin, Herbert W., Biron, Christine A., Ruzek, Melanie C., ...

Blood monocytes or tissue macrophages play a pivotal role in the pathogenesis of murine cytomegalovirus (MCMV) infection, providing functions beneficial to both the virus and the host. In vitro and...

Depletion of Blood-Borne Macrophages Does Not Reduce Demyelination in Mice Infected with a Neurotropic Coronavirus

Xue, Shurong, Sun, Ning, Van Rooijen, Nico, Perlman, Stanley

Mice infected with the neurotropic coronavirus mouse hepatitis virus strain JHM (MHV-JHM) develop a chronic demyelinating disease with symptoms of hindlimb paralysis. Histological examination of the...

Chemokine Receptor CCR2 Is Not Essential for the Development of Experimental Cerebral Malaria

Belnoue, Elodie, Costa, Fabio T. M., Vigário, Ana M., Voza, Tatiana, Gonnet, Françoise, Landau, Irène, ...

Infection with Plasmodium berghei ANKA induces cerebral malaria in susceptible mice. Brain-sequestered CD8+ T cells are responsible for this pathology. We have evaluated the role of CCR2, a chemokine...

The trans-Sialidase from Trypanosoma cruzi Induces Thrombocytopenia during Acute Chagas' Disease by Reducing the Platelet Sialic Acid Contents

Tribulatti, María Virginia, Mucci, Juan, Van Rooijen, Nico, Leguizamón, María Susana, Campetella, Oscar

Strong thrombocytopenia is observed during acute infection with Trypanosoma cruzi, the parasitic protozoan agent of American trypanosomiasis or Chagas' disease. The parasite sheds trans-sialidase, an...

Glycolipid presentation to natural killer T cells differs in an organ-dependent fashion

Schmieg, John, Yang, Guangli, Franck, Richard W., Van Rooijen, Nico, Tsuji, Moriya

It has been shown that dendritic cells (DCs) are able to present glycolipids to natural killer (NK) T cells in vivo. However, the essential role of DCs, as well as the role of other cells in...

Inflammation-induced lymphangiogenesis in the cornea arises from CD11b-positive macrophages

Maruyama, Kazuichi, Ii, Masaaki, Cursiefen, Claus, Jackson, David G., Keino, Hiroshi, Tomita, Minoru, ...

In the inflamed cornea, there is a parallel outgrowth of blood and lymphatic vessels into the normally avascular cornea. We tested whether adaptive and/or innate immune cells were actively involved...

Pathogenicity of Influenza Viruses with Genes from the 1918 Pandemic Virus: Functional Roles of Alveolar Macrophages and Neutrophils in Limiting Virus Replication and Mortality in Mice

Tumpey, Terrence M., García-Sastre, Adolfo, Taubenberger, Jeffery K., Palese, Peter, Swayne, David E., Pantin-Jackwood, Mary J., ...

The Spanish influenza pandemic of 1918 to 1919 swept the globe and resulted in the deaths of at least 20 million people. The basis of the pulmonary damage and high lethality caused by the 1918 H1N1...

Roles of Macrophages and Neutrophils in the Early Host Response to Bacillus anthracis Spores in a Mouse Model of Infection

Cote, Christopher K., Van Rooijen, Nico, Welkos, Susan L.

The development of new approaches to combat anthrax requires that the pathogenesis and host response to Bacillus anthracis spores be better understood. We investigated the roles that macrophages and...

Activated macrophages are essential in a murine model for T cell–mediated chronic psoriasiform skin inflammation

Wang, Honglin, Peters, Thorsten, Kess, Daniel, Sindrilaru, Anca, Oreshkova, Tsvetelina, Van Rooijen, Nico, ...

The CD18 hypomorphic (CD18hypo) PL/J mouse model clinically resembling human psoriasis is characterized by reduced expression of the common chain of β2 integrins (CD11/CD18) to only 2–16% of WT...

Pathogenic role for skin macrophages in a mouse model of keratinocyte-induced psoriasis-like skin inflammation

Stratis, Athanasios, Pasparakis, Manolis, Rupec, Rudolf A., Markur, Doreen, Hartmann, Karin, Scharffetter-Kochanek, Karin, ...

Psoriasis is a common skin disease, the pathogenesis of which has not yet been resolved. In mice, epidermis-specific deletion of inhibitor of NF-κB (IκB) kinase 2 (IKK2) results in a skin phenotype...

Monocyte subsets differentially employ CCR2, CCR5, and CX3CR1 to accumulate within atherosclerotic plaques

Tacke, Frank, Alvarez, David, Kaplan, Theodore J., Jakubzick, Claudia, Spanbroek, Rainer, Llodra, Jaime, ...

Monocytes participate critically in atherosclerosis. There are 2 major subsets expressing different chemokine receptor patterns: CCR2+CX3CR1+Ly-6Chi and CCR2–CX3CR1++Ly-6Clo monocytes. Both C-C...

Conjunctival macrophage-mediated influence of the local and systemic immune response after corneal herpes simplex virus-1 infection

Bauer, Dirk, Schmitz, Andreas, Van Rooijen, Nico, Steuhl, Klaus-Peter, Heiligenhaus, Arnd

Recently it has been shown that selective subconjunctival macrophage depletion reduced the incidence and severity of stromal herpes simplex virus (HSV) keratitis in mice. In this study, we examined...

Role of Inducible Nitric Oxide Synthase and NADPH Oxidase in Early Control of Burkholderia pseudomallei Infection in Mice▿

Breitbach, Katrin , Klocke, Sonja, Tschernig, Thomas, Van Rooijen, Nico, Baumann, Ulrich, Steinmetz, Ivo

Infection with the soil bacterium Burkholderia pseudomallei can result in a variety of clinical outcomes, including asymptomatic infection. The initial immune defense mechanisms which might...

Hypoxia-Induced Pulmonary Vascular Remodeling Requires Recruitment of Circulating Mesenchymal Precursors of a Monocyte/Macrophage Lineage

Frid, Maria G., Brunetti, Jacqueline A., Burke, Danielle L., Carpenter, Todd C., Davie, Neil J., Reeves, John T., ...

Vascular remodeling in chronic hypoxic pulmonary hypertension includes marked fibroproliferative changes in the pulmonary artery (PA) adventitia. Although resident PA fibroblasts have long been...

Distinctive Roles of Neutrophils and Monocytes in Anti-Thy-1 Nephritis

Westerhuis, Ralf, Van Straaten, Suzanne C., Van Rooijen, Nico, Verhagen, Nicole A. M., Dijkstra, Christine D., ...

Anti-Thy-1.1 glomerulonephritis as an experimental model for mesangial proliferative glomerulonephritis was induced in Wistar rats by a single injection of monoclonal IgG2a–anti-Thy-1.1 antibody...

Tumor Necrosis Factor (TNF) Protects Resistant C57BL/6 Mice against Herpes Simplex Virus-Induced Encephalitis Independently of Signaling via TNF Receptor 1 or 2▿

Lundberg, Patric, Welander, Paula V., Edwards, Carl K., Van Rooijen, Nico, Cantin, Edouard

Tumor necrosis factor (TNF) is a multifunctional cytokine that has a role in induction and regulation of host innate and adaptive immune responses. The importance of TNF antiviral mechanisms is...

Importance of Kupffer Cells for T-Cell-Dependent Liver Injury in Mice

Schümann, Jens, Wolf, Dominik, Pahl, Andreas, Brune, Kay, Papadopoulos, Thomas, Van Rooijen, Nico, ...

T cells seem to be responsible for liver damage in any type of acute hepatitis. Nevertheless, the importance of Kupffer cells (KCs) for T-cell-dependent liver failure is unclear. Here we focus on the...

Activator Protein-1 Activation in Acute Lung Injury

Guo, Ren-Feng, Lentsch, Alex B., Sarma, J. Vidya, Sun, Lei, Riedemann, Niels C., McClintock, Shannon D., ...

The role of activator protein-1 (AP-1) in inflammation is primarily unknown. AP-1 was evaluated in nuclear extracts from alveolar macrophages and whole lung nuclear extracts during acute lung injury...

Severe malarial anemia of low parasite burden in rodent models results from accelerated clearance of uninfected erythrocytes

Evans, Krystal J., Hansen, Diana S., Van Rooijen, Nico, Buckingham, Lynn A., Schofield, Louis

Severe malarial anemia (SMA) is the most frequent life-threatening complication of malaria and may contribute to the majority of malarial deaths worldwide. To explore the mechanisms of pathogenesis,...

Pertussis Toxin Targets Airway Macrophages To Promote Bordetella pertussis Infection of the Respiratory Tract▿

Carbonetti, Nicholas H., Artamonova, Galina V., Van Rooijen, Nico, Ayala, Victor I.

Pertussis toxin (PT), a secreted virulence factor of Bordetella pertussis, ADP ribosylates mammalian Gi proteins and plays an important early role in respiratory tract infection by this pathogen in a...

Macrophage Depletion Impairs Wound Healing and Increases Left Ventricular Remodeling after Myocardial Injury in Mice

Van Amerongen, Machteld J., Harmsen, Martin C., Van Rooijen, Nico, Petersen, Arjen H.

Macrophages have been suggested to be beneficial for myocardial wound healing. We investigated the role of macrophages in myocardial wound healing by inhibition of macrophage infiltration after...

Immature monocytes acquire antigens from other cells in the bone marrow and present them to T cells after maturing in the periphery

Tacke, Frank, Ginhoux, Florent, Jakubzick, Claudia, Van Rooijen, Nico, Merad, Miriam, Randolph, Gwendalyn J.

Monocytes are circulating precursors for tissue macrophages and dendritic cells (DCs) but are not recognized to directly participate in antigen presentation. We developed techniques to label mouse...

Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis

Agrawal, Smriti, Anderson, Per, Durbeej, Madeleine, Van Rooijen, Nico, Ivars, Fredrik, Opdenakker, Ghislain, ...

The endothelial cell monolayer of cerebral vessels and its basement membrane (BM) are ensheathed by the astrocyte endfeet, the leptomeningeal cells, and their associated parenchymal BM, all of which...

Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis

Arnold, Ludovic, Henry, Adeline, Poron, Françoise, Baba-Amer, Yasmine, Van Rooijen, Nico, Plonquet, Anne, ...

Macrophages (MPs) are important for skeletal muscle regeneration in vivo and may exert beneficial effects on myogenic cell growth through mitogenic and antiapoptotic activities in vitro. However, MPs...

Lack of CD47 on nonhematopoietic cells induces split macrophage tolerance to CD47null cells

Wang, Hui, Madariaga, Maria Lucia, Wang, Shumei, Van Rooijen, Nico, Oldenborg, Per-Arne, Yang, Yong-Guang

Macrophages recognize CD47 as a marker of “self” and phagocytose CD47null hematopoietic cells. Using CD47 chimera models, here, we show that the phagocytic activity of macrophages against...

An antigen-specific pathway for CD8 T cells across the blood-brain barrier

Galea, Ian, Bernardes-Silva, Martine, Forse, Penny A., Van Rooijen, Nico, Liblau, Roland S., Perry, V. Hugh

CD8 T cells are nature's foremost defense in encephalitis and brain tumors. Antigen-specific CD8 T cells need to enter the brain to exert their beneficial effects. On the other hand, traffic of CD8 T...

Role of CCR8 and Other Chemokine Pathways in the Migration of Monocyte-derived Dendritic Cells to Lymph Nodes

Qu, Chunfeng, Edwards, Emmerson W., Tacke, Frank, Angeli, Véronique, Llodrá, Jaime, Sanchez-Schmitz, Guzman, ...

Studying the influence of chemokine receptors (CCRs) on monocyte fate may reveal information about which subpopulations of monocytes convert to dendritic cells (DCs) and the migration pathways that...

Macrophage-Mediated but Gamma Interferon-Independent Innate Immune Responses Control the Primary Wave of Plasmodium yoelii Parasitemia▿

Couper, Kevin N., Blount, Daniel G., Hafalla, Julius C. R., Van Rooijen, Nico, De Souza, J. Brian, Riley, Eleanor M.

In most models of blood-stage malaria infection, proinflammatory immune responses are required for control of infection and elimination of parasites. We hypothesized therefore that the fulminant...

Gamma Interferon-Independent Effects of Interleukin-12 on Immunity to Salmonella enterica Serovar Typhimurium▿

Price, Jason D., Simpfendorfer, Kim R., Mantena, Radhakrishnam R., Holden, James, Heath, William R., Van Rooijen, Nico, ...

Interleukin-12 (IL-12) and IL-18 are both central to the induction of gamma interferon (IFN-γ), and various roles for IL-12 and IL-18 in control of intracellular microbial infections have been...

A Conduit System Distributes Chemokines and Small Blood-borne Molecules through the Splenic White Pulp

Nolte, Martijn A., Beliën, Jeroen A.M., Schadee-Eestermans, Inge, Jansen, Wendy, Unger, Wendy W.J., Van Rooijen, Nico, ...

Access to the splenic white pulp is restricted to lymphocytes and dendritic cells. Here we show that movement of molecules from the blood into these confined areas is also limited. Large molecules,...

The Role of Macrophages in T Cell–mediated Autoimmune Diabetes in Nonobese Diabetic Mice

Jun, Hee-Sook, Yoon, Chang-Soon, Zbytnuik, Lori, Van Rooijen, Nico, Yoon, Ji-Won

We have shown previously that the inactivation of macrophages in nonobese diabetic (NOD) mice results in the prevention of diabetes; however, the mechanisms involved remain unknown. In this study, we...

Human Malaria in Immunocompromised Mice: An in Vivo Model to Study Defense Mechanisms against Plasmodium falciparum

Badell, Edgar, Oeuvray, Claude, Moreno, Alicia, Soe, Soe, Van Rooijen, Nico, Bouzidi, Ahmed, ...

We have recently described that sustained Plasmodium falciparum growth could be obtained in immunodeficient mice. We now report the potential of this new mouse model by assaying the effect of the...

Mucosal Damage and Neutropenia Are Required for Candida albicans Dissemination

Koh, Andrew Y, Köhler, Julia R, Coggshall, Kathleen T, Van Rooijen, Nico, Pier, Gerald B

Candida albicans fungemia in cancer patients is thought to develop from initial gastrointestinal (GI) colonization with subsequent translocation into the bloodstream after administration of...

Alveolar Macrophages Are Indispensable for Controlling Influenza Viruses in Lungs of Pigs▿

Kim, Heui Man, Lee, Young-Won, Lee, Ki-Ja, Kim, Hyun Soo, Cho, Sung Whan, Van Rooijen, Nico, ...

Alveolar macrophages constitutively reside in the respiratory tracts of pigs and humans. An in vivo role of alveolar macrophages in defending against influenza viruses in mice infected with a...

Macrophages Are Mediators of Gastritis in Acute Helicobacter pylori Infection in C57BL/6 Mice▿

Kaparakis, Maria, Walduck, Anna K., Price, Jason D., Pedersen, John S., Van Rooijen, Nico, Pearse, Martin J., ...

Helicobacter pylori is the etiological agent of human chronic gastritis, a condition seen as a precursor to the development of gastrointestinal ulcers or gastric cancer. This study utilized the...

Protective influenza-specific CD8 T cell responses require interactions with dendritic cells in the lungs

McGill, Jodi, Van Rooijen, Nico, Legge, Kevin L.

Influenza infections induce a rapid, but transient, dendritic cell (DC) migration from the lungs to the lymph nodes (LNs) that is followed by substantial recruitment of DCs into the lungs without...

Extratumoral Macrophages Promote Tumor and Vascular Growth in an Orthotopic Rat Prostate Tumor Model1

Halin, Sofia, Rudolfsson, Stina Häggström, Van Rooijen, Nico, Bergh, Anders

Tumor-associated macrophages are involved in angiogenesis and tumor progression, but their role and specific site of action in prostate cancer remain unknown. To explore this, Dunning R-3327 AT-1 rat...

Resolution-phase macrophages possess a unique inflammatory phenotype that is controlled by cAMP

Bystrom, Jonas, Evans, Ian, Newson, Justine, Stables, Melanie, Toor, Iqbal, Van Rooijen, Nico, ...

Neutralizing injurious stimuli, proinflammatory mediator catabolism, and polymorphonuclear leukocyte (PMN) clearance are determinants of inflammatory resolution. To this, we recently added...

Ly6c+ “inflammatory monocytes” are microglial precursors recruited in a pathogenic manner in West Nile virus encephalitis

Getts, Daniel R., Terry, Rachael L., Getts, Meghann Teague, Müller, Marcus, Rana, Sabita, Shrestha, Bimmi, ...

In a lethal West Nile virus (WNV) model, central nervous system infection triggered a threefold increase in CD45int/CD11b+/CD11c− microglia at days 6–7 postinfection (p.i.). Few microglia were...

Use of Tissue-Specific MicroRNA to Control Pathology of Wild-Type Adenovirus without Attenuation of Its Ability to Kill Cancer Cells

Cawood, Ryan, Chen, Hannah H., Carroll, Fionnadh, Bazan-Peregrino, Miriam, Van Rooijen, Nico, Seymour, Leonard W.

Replicating viruses have broad applications in biomedicine, notably in cancer virotherapy and in the design of attenuated vaccines; however, uncontrolled virus replication in vulnerable tissues can...

MyD88-dependent, superoxide-initiated inflammation is necessary for flow-mediated inward remodeling of conduit arteries

Tang, Paul C.Y., Qin, Lingfeng, Zielonka, Jacek, Zhou, Jing, Matte-Martone, Catherine, Bergaya, Sonia, ...

Vascular remodeling normalizes abnormal hemodynamic stresses through structural changes affecting vessel size and wall thickness. We investigated the role of inflammation in flow-mediated vascular...

TLR7-dependent and FcγR-independent production of type I interferon in experimental mouse lupus

Lee, Pui Y., Kumagai, Yutaro, Li, Yi, Takeuchi, Osamu, Yoshida, Hideo, Weinstein, Jason, ...

Increased type I interferon (IFN-I) production and IFN-stimulated gene (ISG) expression are linked to the pathogenesis of systemic lupus erythematosus (SLE). Although the mechanisms responsible for...

Effect of Neutralizing Sera on Factor X-Mediated Adenovirus Serotype 5 Gene Transfer▿

Parker, Alan L., Waddington, Simon N., Buckley, Suzanne M. K., Custers, Jerome, Havenga, Menzo J. E., Van Rooijen, Nico, ...

The deployment of adenovirus serotype 5 (Ad5)-based vectors is hampered by preexisting immunity. When such vectors are delivered intravenously, hepatocyte transduction is mediated by the...

Hepatic Mrp4 induction following acetaminophen exposure is dependent on Kupffer cell function

Campion, Sarah N., Johnson, Rachel, Aleksunes, Lauren M., Goedken, Michael J., Van Rooijen, Nico, Scheffer, George L., ...

During acetaminophen (APAP) hepatotoxicity, increased expression of multidrug resistance-associated proteins 2, 3, and 4 (Mrp2-4) occurs. Mrp4 is the most significantly upregulated transporter in...

A Novel Animal Model of Borrelia recurrentis Louse-Borne Relapsing Fever Borreliosis Using Immunodeficient Mice

Larsson, Christer, Lundqvist, Jenny, Van Rooijen, Nico, Bergström, Sven

Louse-borne relapsing fever (LBRF) borreliosis is caused by Borrelia recurrentis, and it is a deadly although treatable disease that is endemic in the Horn of Africa but has epidemic potential....

Umbilical cord blood regulatory T-cell expansion and functional effects of tumor necrosis factor receptor family members OX40 and 4-1BB expressed on artificial antigen-presenting cells

Hippen, Keli L., Harker-Murray, Paul, Porter, Stephen B., Merkel, Sarah C., Londer, Aryel, Taylor, Dawn K., ...

Previously, we showed that human umbilical cord blood (UCB) regulatory T cells (Tregs) could be expanded approximately 100-fold using anti-CD3/28 monoclonal antibody (mAb)–coated beads to provide...

Evasion by Stealth: Inefficient Immune Activation Underlies Poor T Cell Response and Severe Disease in SARS-CoV-Infected Mice

Zhao, Jincun, Zhao, Jingxian, Van Rooijen, Nico, Perlman, Stanley

Severe Acute Respiratory Syndrome caused substantial morbidity and mortality during the 2002–2003 epidemic. Many of the features of the human disease are duplicated in BALB/c mice infected with a...

Role of Lung-marginated Monocytes in an In Vivo Mouse Model of Ventilator-induced Lung Injury

Wilson, Michael R., O'Dea, Kieran P., Zhang, Da, Shearman, Alexander D., Van Rooijen, Nico, Takata, Masao

Rationale: Recruited leukocytes play an important role in ventilator-induced lung injury, although studies have focused predominantly on neutrophils. Inflammatory subset Gr-1high monocytes are...