Nicole Gas

The pre-ribosomal network (2003)

Milkereit, Philipp, Kühn, Holger, Gas, Nicole, Tschochner, Herbert

Recent achievements in yeast functional proteomics have significantly advanced our knowledge about ribosome biogenesis. Here, we present a program developed to integrate data from various proteome...

Assembly and Functional Organization of the Nucleolus: Ultrastructural Analysis of Saccharomyces cerevisiae Mutants

Trumtel, Stéphanie, Léger-Silvestre, Isabelle, Gleizes, Pierre-Emmanuel, Teulières, Frédéric, Gas, Nicole

Using Saccharomyces cerevisiae strains with genetically modified nucleoli, we show here that changing parameters as critical as the tandem organization of the ribosomal genes and the polymerase...

The Role of the Schizosaccharomyces pombe gar2 Protein in Nucleolar Structure and Function Depends on the Concerted Action of its Highly Charged N Terminus and its RNA-binding Domains

Sicard, Hélène, Faubladier, Marlène, Noaillac-Depeyre, Jacqueline, Léger-Silvestre, Isabelle, Gas, Nicole, Caizergues-Ferrer, Michèle

Nonribosomal nucleolar protein gar2 is required for 18S rRNA and 40S ribosomal subunit production in Schizosaccharomyces pombe. We have investigated the consequences of the absence of each structural...

Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps

Saveanu, Cosmin, Bienvenu, David, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Gas, Nicole, Jacquier, Alain, ...

Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p...

SURVEY AND SUMMARY: The pre-ribosomal network

Milkereit, Philipp, Kühn, Holger, Gas, Nicole, Tschochner, Herbert

Recent achievements in yeast functional proteomics have significantly advanced our knowledge about ribosome biogenesis. Here, we present a program developed to integrate data from various proteome...

Sequential Protein Association with Nascent 60S Ribosomal Particles

Saveanu, Cosmin, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Lebreton, Alice, Rousselle, Jean-Claude, Noaillac-Depeyre, Jacqueline, ...

Ribosome biogenesis in eukaryotes depends on the coordinated action of ribosomal and nonribosomal proteins that guide the assembly of preribosomal particles. These intermediate particles follow a...

The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast

Léger-Silvestre, Isabelle, Milkereit, Philipp, Ferreira-Cerca, Sébastien, Saveanu, Cosmin, Rousselle, Jean-Claude, Choesmel, Valérie, ...

We have conducted a genetic screen in order to identify ribosomal proteins of Saccharomyces cerevisiae involved in nuclear export of the small subunit precursors. This has led us to distinguish...

Assembly and Functional Organization of the Nucleolus: Ultrastructural Analysis of Saccharomyces cerevisiae Mutants

Trumtel, Stéphanie, Léger-Silvestre, Isabelle, Gleizes, Pierre-Emmanuel, Teulières, Frédéric, Gas, Nicole

Using Saccharomyces cerevisiae strains with genetically modified nucleoli, we show here that changing parameters as critical as the tandem organization of the ribosomal genes and the polymerase...

The Role of the Schizosaccharomyces pombe gar2 Protein in Nucleolar Structure and Function Depends on the Concerted Action of its Highly Charged N Terminus and its RNA-binding Domains

Sicard, Hélène, Faubladier, Marlène, Noaillac-Depeyre, Jacqueline, Léger-Silvestre, Isabelle, Gas, Nicole, Caizergues-Ferrer, Michèle

Nonribosomal nucleolar protein gar2 is required for 18S rRNA and 40S ribosomal subunit production in Schizosaccharomyces pombe. We have investigated the consequences of the absence of each structural...

Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps

Saveanu, Cosmin, Bienvenu, David, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Gas, Nicole, Jacquier, Alain, ...

Eukaryotic ribosome maturation depends on a set of well ordered processing steps. Here we describe the functional characterization of yeast Nog2p (Ynr053cp), a highly conserved nuclear protein. Nog2p...

SURVEY AND SUMMARY: The pre-ribosomal network

Milkereit, Philipp, Kühn, Holger, Gas, Nicole, Tschochner, Herbert

Recent achievements in yeast functional proteomics have significantly advanced our knowledge about ribosome biogenesis. Here, we present a program developed to integrate data from various proteome...

Sequential Protein Association with Nascent 60S Ribosomal Particles

Saveanu, Cosmin, Namane, Abdelkader, Gleizes, Pierre-Emmanuel, Lebreton, Alice, Rousselle, Jean-Claude, Noaillac-Depeyre, Jacqueline, ...

Ribosome biogenesis in eukaryotes depends on the coordinated action of ribosomal and nonribosomal proteins that guide the assembly of preribosomal particles. These intermediate particles follow a...

The ribosomal protein Rps15p is required for nuclear exit of the 40S subunit precursors in yeast

Léger-Silvestre, Isabelle, Milkereit, Philipp, Ferreira-Cerca, Sébastien, Saveanu, Cosmin, Rousselle, Jean-Claude, Choesmel, Valérie, ...

We have conducted a genetic screen in order to identify ribosomal proteins of Saccharomyces cerevisiae involved in nuclear export of the small subunit precursors. This has led us to distinguish...

Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the Pre-rRNA Processing Pathway

Dechampesme, Anne-Marie, Koroleva, Olga, Leger-Silvestre, Isabelle, Gas, Nicole, Camier, Sylvie

A collection of yeast strains surviving with mutant 5S RNA has been constructed. The mutant strains presented alterations of the nucleolar structure, with less granular component, and a...

Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex

Gleizes, Pierre-Emmanuel, Noaillac-Depeyre, Jacqueline, Léger-Silvestre, Isabelle, Teulières, Frédéric, Dauxois, Jean-Yves, Pommet, Denys, ...

To study the nuclear export of preribosomes, ribosomal RNAs were detected by in situ hybridization using fluorescence and EM, in the yeast Saccharomyces cerevisiae. In wild-type cells,...

Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing

Gadal, Olivier, Strauss, Daniela, Petfalski, Elisabeth, Gleizes, Pierre-Emmanuel, Gas, Nicole, Tollervey, David, ...

Many analyses have examined subnucleolar structures in eukaryotic cells, but the relationship between morphological structures, pre-rRNA processing, and ribosomal particle assembly has remained...