Nobutaka Hirokawa

Analysis of the mouse transcriptome for genes involved in the function of the nervous system (2003)

Gustincich, Stefano, Batalov, Serge, Beisel, Kirk W., Bona, Hidemasa, Carninci, Piero, Fletcher, Colin F., ...

We analyzed the mouse Representative Transcript and Protein Set for molecules involved in brain function. We found full-length cDNAs of many known brain genes and discovered new members of known...

Analysis of the Mouse Transcriptome for Genes Involved in the Function of the Nervous System (2003)

Gustincich, Stefano, Batalov, Serge, Beisel, Kirk W., Bono, Hidemasa, Carninci, Piero, Fletcher, Colin F., ...

We analyzed the mouse Representative Transcript and Protein Set for molecules involved in brain function.We found full-length cDNAs of many known brain genes and discovered new members of known brain...

Kinesin Superfamily Proteins (KIFs) in the Mouse Transcriptome (2003)

Miki, Harukata, Setou, Mitsutoshi, GSL Members,, Hirokawa, Nobutaka

In the post genomic era where virtually all the genes and the proteins are known, an important task is to provide a comprehensive analysis of the expression of important classes of genes, such as...

Mechanism of the single-headed processivity: Diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin

Okada, Yasushi, Hirokawa, Nobutaka

A motor-domain construct of KIF1A, a single-headed kinesin superfamily protein, was demonstrated to take more than 600 steps before detaching from a microtubule. However, its molecular mechanism...

Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome

Nakagawa, Terunaga, Tanaka, Yosuke, Matsuoka, Eiji, Kondo, Satoru, Okada, Yasushi, Noda, Yasuko, ...

KIF (kinesin superfamily) proteins are microtubule-dependent molecular motors that play important roles in intracellular transport and cell division. The extent to which KIFs are involved in various...

All kinesin superfamily protein, KIF, genes in mouse and human

Miki, Harukata, Setou, Mitsutoshi, Kaneshiro, Kiyofumi, Hirokawa, Nobutaka

Intracellular transport is essential for morphogenesis and functioning of the cell. The kinesin superfamily proteins (KIFs) have been shown to transport membranous organelles and protein complexes in...

Binding of Murine Leukemia Virus Gag Polyproteins to KIF4, a Microtubule-Based Motor Protein

Kim, Wankee, Tang, Yao, Okada, Yasushi, Torrey, Ted A., Chattopadhyay, Sisir K., Pfleiderer, Michael, ...

A cDNA clone encoding a cellular protein that interacts with murine leukemia virus (MuLV) Gag proteins was isolated from a T-cell lymphoma library. The sequence of the clone is identical to the C...

Molecular Motor KIF1C Is Not Essential for Mouse Survival and Motor-Dependent Retrograde Golgi Apparatus-to-Endoplasmic Reticulum Transport

Nakajima, Kazuo, Takei, Yosuke, Tanaka, Yosuke, Nakagawa, Terunaga, Nakata, Takao, Noda, Yasuko, ...

KIF1C is a new member of the kinesin superfamily of proteins (KIFs), which act as microtubule-based molecular motors involved in intracellular transport. We cloned full-length mouse kif1C cDNA, which...

Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice

Wong, Richard Wing-Chuen, Setou, Mitsutoshi, Teng, Junlin, Takei, Yosuke, Hirokawa, Nobutaka

The kinesin superfamily proteins (KIFs) play essential roles in receptor transportation along the microtubules. KIF17 transports the N-methyl-d-aspartate receptor NR2B subunit in vitro, but its role...

Analysis of the Mouse Transcriptome for Genes Involved in the Function of the Nervous System

Gustincich, Stefano, Batalov, Serge, Beisel, Kirk W., Bono, Hidemasa, Carninci, Piero, Fletcher, Colin F., ...

We analyzed the mouse Representative Transcript and Protein Set for molecules involved in brain function.We found full-length cDNAs of many known brain genes and discovered new members of known brain...

Kinesin Superfamily Proteins (KIFs) in the Mouse Transcriptome

Miki, Harukata, Setou, Mitsutoshi, Hirokawa, Nobutaka

In the post genomic era where virtually all the genes and the proteins are known, an important task is to provide a comprehensive analysis of the expression of important classes of genes, such as...

Dynein and kinesin share an overlapping microtubule-binding site

Mizuno, Naoko, Toba, Shiori, Edamatsu, Masaki, Watai-Nishii, Junko, Hirokawa, Nobutaka, Toyoshima, Yoko Y, ...

Dyneins and kinesins move in opposite directions on microtubules. The question of how the same-track microtubules are able to support movement in two directions remains unanswered due to the absence...

Nodal Cilia Dynamics and the Specification of the Left/Right Axis in Early Vertebrate Embryo Development

Buceta, Javier, Ibañes, Marta, Rasskin-Gutman, Diego, Okada, Yasushi, Hirokawa, Nobutaka, Izpisúa-Belmonte, Juan Carlos

Nodal cilia dynamics is a key factor for left/right axis determination in mouse embryos through the induction of a leftward fluid flow. So far it has not been clearly established how such dynamics is...

Mechanism of the single-headed processivity: Diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin

Okada, Yasushi, Hirokawa, Nobutaka

A motor-domain construct of KIF1A, a single-headed kinesin superfamily protein, was demonstrated to take more than 600 steps before detaching from a microtubule. However, its molecular mechanism...

Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome

Nakagawa, Terunaga, Tanaka, Yosuke, Matsuoka, Eiji, Kondo, Satoru, Okada, Yasushi, Noda, Yasuko, ...

KIF (kinesin superfamily) proteins are microtubule-dependent molecular motors that play important roles in intracellular transport and cell division. The extent to which KIFs are involved in various...

All kinesin superfamily protein, KIF, genes in mouse and human

Miki, Harukata, Setou, Mitsutoshi, Kaneshiro, Kiyofumi, Hirokawa, Nobutaka

Intracellular transport is essential for morphogenesis and functioning of the cell. The kinesin superfamily proteins (KIFs) have been shown to transport membranous organelles and protein complexes in...

Binding of Murine Leukemia Virus Gag Polyproteins to KIF4, a Microtubule-Based Motor Protein

Kim, Wankee, Tang, Yao, Okada, Yasushi, Torrey, Ted A., Chattopadhyay, Sisir K., Pfleiderer, Michael, ...

A cDNA clone encoding a cellular protein that interacts with murine leukemia virus (MuLV) Gag proteins was isolated from a T-cell lymphoma library. The sequence of the clone is identical to the C...

Molecular Motor KIF1C Is Not Essential for Mouse Survival and Motor-Dependent Retrograde Golgi Apparatus-to-Endoplasmic Reticulum Transport

Nakajima, Kazuo, Takei, Yosuke, Tanaka, Yosuke, Nakagawa, Terunaga, Nakata, Takao, Noda, Yasuko, ...

KIF1C is a new member of the kinesin superfamily of proteins (KIFs), which act as microtubule-based molecular motors involved in intracellular transport. We cloned full-length mouse kif1C cDNA, which...

Overexpression of motor protein KIF17 enhances spatial and working memory in transgenic mice

Wong, Richard Wing-Chuen, Setou, Mitsutoshi, Teng, Junlin, Takei, Yosuke, Hirokawa, Nobutaka

The kinesin superfamily proteins (KIFs) play essential roles in receptor transportation along the microtubules. KIF17 transports the N-methyl-d-aspartate receptor NR2B subunit in vitro, but its role...

Analysis of the Mouse Transcriptome for Genes Involved in the Function of the Nervous System

Gustincich, Stefano, Batalov, Serge, Beisel, Kirk W., Bono, Hidemasa, Carninci, Piero, Fletcher, Colin F., ...

We analyzed the mouse Representative Transcript and Protein Set for molecules involved in brain function.We found full-length cDNAs of many known brain genes and discovered new members of known brain...

Kinesin Superfamily Proteins (KIFs) in the Mouse Transcriptome

Miki, Harukata, Setou, Mitsutoshi, Hirokawa, Nobutaka

In the post genomic era where virtually all the genes and the proteins are known, an important task is to provide a comprehensive analysis of the expression of important classes of genes, such as...

Dynein and kinesin share an overlapping microtubule-binding site

Mizuno, Naoko, Toba, Shiori, Edamatsu, Masaki, Watai-Nishii, Junko, Hirokawa, Nobutaka, Toyoshima, Yoko Y, ...

Dyneins and kinesins move in opposite directions on microtubules. The question of how the same-track microtubules are able to support movement in two directions remains unanswered due to the absence...

Nodal Cilia Dynamics and the Specification of the Left/Right Axis in Early Vertebrate Embryo Development

Buceta, Javier, Ibañes, Marta, Rasskin-Gutman, Diego, Okada, Yasushi, Hirokawa, Nobutaka, Izpisúa-Belmonte, Juan Carlos

Nodal cilia dynamics is a key factor for left/right axis determination in mouse embryos through the induction of a leftward fluid flow. So far it has not been clearly established how such dynamics is...

High-resolution cryo-EM maps show the nucleotide binding pocket of KIF1A in open and closed conformations

Kikkawa, Masahide, Hirokawa, Nobutaka

Kinesin is an ATP-driven microtubule (MT)-based motor fundamental to organelle transport. Although a number of kinesin crystal structures have been solved, the structural evidence for coupling...

Three-dimensional structures of the flagellar dynein–microtubule complex by cryoelectron microscopy

Oda, Toshiyuki, Hirokawa, Nobutaka, Kikkawa, Masahide

The outer dynein arms (ODAs) of the flagellar axoneme generate forces needed for flagellar beating. Elucidation of the mechanisms underlying the chemomechanical energy conversion by the dynein arms...

A standardized kinesin nomenclature

Lawrence, Carolyn J., Dawe, R. Kelly, Christie, Karen R., Cleveland, Don W., Dawson, Scott C., Endow, Sharyn A., ...

In recent years the kinesin superfamily has become so large that several different naming schemes have emerged, leading to confusion and miscommunication. Here, we set forth a standardized kinesin...

Impairment of Inhibitory Synaptic Transmission in Mice Lacking Synapsin I

Terada, Sumio, Tsujimoto, Tetsuhiro, Takei, Yosuke, Takahashi, Tomoyuki, Hirokawa, Nobutaka

Deletion of the synapsin I genes, encoding one of the major groups of proteins on synaptic vesicles, in mice causes late onset epileptic seizures and enhanced experimental temporal lobe epilepsy....

Left-Right Asymmetry and Kinesin Superfamily Protein KIF3A: New Insights in Determination of Laterality and Mesoderm Induction by kif3A−/− Mice Analysis

Takeda, Sen, Yonekawa, Yoshiaki, Tanaka, Yosuke, Okada, Yasushi, Nonaka, Shigenori, Hirokawa, Nobutaka

KIF3A is a classical member of the kinesin superfamily proteins (KIFs), ubiquitously expressed although predominantly in neural tissues, and which forms a heterotrimeric KIF3 complex with KIF3B or...

Delayed Development of Nervous System in Mice Homozygous for Disrupted Microtubule-associated Protein 1B (MAP1B) Gene

Takei, Yosuke, Kondo, Satoru, Harada, Akihiro, Inomata, Satomi, Noda, Tetsuo, Hirokawa, Nobutaka

Microtubule-associated protein 1B (MAP1B), one of the microtubule-associated proteins (MAPs), is a major component of the neuronal cytoskeleton. It is expressed at high levels in immature neurons...

Visualization of the Dynamics of Synaptic Vesicle and Plasma Membrane Proteins in Living Axons

Nakata, Takao, Terada, Sumio, Hirokawa, Nobutaka

Newly synthesized membrane proteins are transported by fast axonal flow to their targets such as the plasma membrane and synaptic vesicles. However, their transporting vesicles have not yet been...

Defect in Synaptic Vesicle Precursor Transport and Neuronal Cell Death in KIF1A Motor Protein–deficient Mice

Yonekawa, Yoshiaki, Harada, Akihiro, Okada, Yasushi, Funakoshi, Takeshi, Kanai, Yoshimitsu, Takei, Yosuke, ...

The nerve axon is a good model system for studying the molecular mechanism of organelle transport in cells. Recently, the new kinesin superfamily proteins (KIFs) have been identified as candidate...

Synergistic effects of MAP2 and MAP1B knockout in neuronal migration, dendritic outgrowth, and microtubule organization

Teng, Junlin, Takei, Yosuke, Harada, Akihiro, Nakata, Takao, Chen, Jianguo, Hirokawa, Nobutaka

MAP1B and MAP2 are major members of neuronal microtubule-associated proteins (MAPs). To gain insights into the function of MAP2 in vivo, we generated MAP2-deficient (map2−/−) mice. They developed...

KIFC3, a microtubule minus end–directed motor for the apical transport of annexin XIIIb–associated Triton-insoluble membranes

Noda, Yasuko, Okada, Yasushi, Saito, Nobuhito, Setou, Mitsutoshi, Xu, Ying, Zhang, Zheizeng, ...

We have identified and characterized a COOH-terminal motor domain–type kinesin superfamily protein (KIFC), KIFC3, in the kidney. KIFC3 is a minus end–directed microtubule motor protein, therefore...

Microtubules provide directional cues for polarized axonal transport through interaction with kinesin motor head

Nakata, Takao, Hirokawa, Nobutaka

Post-Golgi carriers of various newly synthesized axonal membrane proteins, which possess kinesin (KIF5)-driven highly processive motility, were transported from the TGN directly to axons. We found...

Role of KIFC3 motor protein in Golgi positioning and integration

Xu, Ying, Takeda, Sen, Nakata, Takao, Noda, Yasuko, Tanaka, Yosuke, Hirokawa, Nobutaka

KIFC3, a microtubule (MT) minus end–directed kinesin superfamily protein, is expressed abundantly and is associated with the Golgi apparatus in adrenocortical cells. We report here that disruption...

MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and proper PKA signal transduction

Harada, Akihiro, Teng, Junlin, Takei, Yosuke, Oguchi, Keiko, Hirokawa, Nobutaka

Microtubule-associated protein 2 (MAP2) is a major component of cross-bridges between microtubules in dendrites, and is known to stabilize microtubules. MAP2 also has a binding domain for the...

Kinesin Superfamily Protein 3 (Kif3) Motor Transports Fodrin-Associating Vesicles Important for Neurite Building

Takeda, Sen, Yamazaki, Hiroto, Seog, Dae-Hyun, Kanai, Yoshimitsu, Terada, Sumio, Hirokawa, Nobutaka

Kinesin superfamily proteins (KIFs) comprise several dozen molecular motor proteins. The KIF3 heterotrimer complex is one of the most abundantly and ubiquitously expressed KIFs in mammalian cells. To...

Defects in Axonal Elongation and Neuronal Migration in Mice with Disrupted tau and map1b Genes

Takei, Yosuke, Teng, Junlin, Harada, Akihiro, Hirokawa, Nobutaka

Tau and MAP1B are the main members of neuronal microtubule-associated proteins (MAPs), the functions of which have remained obscure because of a putative functional redundancy (Harada, A., K. Oguchi,...

Left–Right Determination: Involvement of Molecular Motor KIF3, Cilia, and Nodal Flow

Hirokawa, Nobutaka, Tanaka, Yosuke, Okada, Yasushi

Mammalian left–right determination is a good example for how multiple cell biological processes coordinate in the formation of a basic body plan. The leftward movement of fluid at the ventral node,...