The accumulation of a glucosphingolipid (GSL) in individuals lacking an adequate level of hydrolase activity could be minimized by chemotherapeutic measures that slow the formation of the GSL and...
Chatterjee, Subroto, Cleveland, Tavia, Shi, Wan-Yang, Inokuchi, Jin-Ichi, Radin, Norman S.
We have studied the effects of D -threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol ( D -PDMP) and its L -enantiomer on glycosphingolipids in cultured normal human kidney proximal tubular cells....
Treatment of Gaucher disease with an enzyme inhibitor (1996)
The hypothesis is offered predicting that Caucher patients could be treated with a drug that slows the synthesis of glucosylceramide, the lipid that accumulates in this disorder. The present...
Glucosylceramide in the nervous system - A mini-review (1994)
A reviewer of this manuscript has recommended that I warn the reader that the hypotheses offered here do not have enough experimental support to make them widely accepted.
Studies on morpholinosphingolipids: Potent inhibitors of glucosylceramide synthase (1994)
Carson, Kenneth G., Radin, Norman S., Abe, Akira, Shayman, James A.
Synthetic 1-morpholino-1-deoxyceramides were designed to inhibit glucosylceramide synthase. The most potent inhibitor 2a possesses the unnatural R,R-configuration of D-threo-sphingosine.
Mahdiyoun, Shahryar, Deshmukh, Gayatri D., Abe, Akira, Radin, Norman S., Shayman, James A.
Recent work has demonstrated the enhancement of hormone-stimulated inositol trisphosphate formation in renal epithelial cells under conditions of glucosylceramide depletion. The role of...
A ceramide analogue (PDMP) inhibits glycolipid synthesis in fish embryos (1992)
Fenderson, Bruce A., Ostrander, Gary K., Hausken, Zach, Radin, Norman S., Hakomori, Sen-itiroh
Glycolipids were depleted from medaka embryos using 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), an inhibitor of glucosylceramide synthetase. Embryos cultured in the presence of 20 [mu]M...
Improved Inhibitors of Glucosylceramide Synthase (1992)
Abe, Akira, Inokuchi, Jin-ichi, Jimbo, Masayuki, Shimeno, Hiroshi, Nagamatsu, Atsuo, Shayman, James A., ...
An inhibitor of glucosylceramide (GlcCer) synthase, l-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), has been reported to deplete cells and mice of their glucosphingolipids. This inhibitor...
Shukla, Girja S., Shukla, Arti, Inokuchi, Jin-Ichi, Radin, Norman S.
The ceramide analog, -threo-1-phenyl-2-decanoylamino-3-morpholino-1-morpholino-1-propanol, inhibits the glucosylation of ceramide and thus, by virtue of the normal catabolism of the higher...
Shukla, Girja S., Radin, Norman S.
The synthesis of glucosylceramide from ceramide and UDP-[3H]glucose by mouse kidney homogenates is very sensitive to the concentration of tissue. This was shown to be due to the presence of a UDP-glc...
The carbohydrate moiety of the activator protein for glucosylceramide [beta]-glucosidase (1988)
SAP-2 is a family of heat-stable, acidic glycoproteins which stimulate enzymatic hydrolysis of glucosylceramide. We studied the carbohydrate moieties of a ConA-binding form of SAP-2. The protein...
Okada, Yoshio, Radin, Norman S., Hakomori, Sen-itiroh
A culture of BALB/c 3T3 cells grown in the presence of 40 [mu]M of the ceramide analog compound RV538 (2-decanoylamino-3-morpholino-1-phenylpropanol) for several passages caused a substantial...
Datta, Subhash C., Radin, Norman S.
A model of the human genetic disorder, Gaucher disease, can be rapidly generated in mice by the injection of emulsified glucosylceramide and an inhibitor of the lipid's hydrolase, conduritol B...
Antitumor activity via inhibition of glycosphingolipid biosynthesis (1987)
Inokuchi, Jin-Ichi, Mason, Inez, Radin, Norman S.
The production by cancer cells of glycolipids, perhaps derived partly from host glycolipids, may play essential roles in malignancy, tumor growth, immunity from host immunodefense, and metastasis....
Datta, Subhash C., Snider, R. Michael, Radin, Norman S.
Serum-free cultured neuroblastoma cells (clone NIE-115) have been shown to absorb emulsified glucosylceramide, glucosylceramide glucosidase, an activator protein for the enzyme, and...
Radin, Norman S., Klinger, Paul
The standard method for selecting experimental animals for control and experimental groups is by a randomization process in which each animal is placed into one of the groups without looking at any...
Improved synthesis of conduritol B epoxide (1985)
Lee, Kevin J., Boyd, Steven A., Radin, Norman S.
Peer Reviewed
Quantitation of thin-layer chromatograms with an Apple II computer-based videodensitometer (1985)
Ford-Holevinski, Thomas S., Radin, Norman S.
An inexpensive, high-speed densitometer made from an Apple II computer, a black-and-white video camera, and an image digitizing board is described. By supplementing the computer with a very fast...
Datta, Subhash C., Radin, Norman S.
A procedure for the immunoassay of cohydrolase sphingolipid-I in mouse tissue is described. This cohydrolase (actually a mixture of at least four related proteins) stimulates or activates the...
Black, Keith L., Hsu, S., Radin, Norman S., Hoff, Julian T.
Eicosapentaenoic acid is converted by cyclo-oxygenase to the prostacyclin, PGI3. Consequently eicosapentaenoic acid might protect the brain from the impairment in cerebral blood flow that follows...
The cohydrolases in human spleen that stimulate glucosyl ceramide [beta]-glucosidase (1983)
Iyer, Shankar S., Berent, Susan L., Radin, Norman S.
A family of [beta]-glucosidase-stimulating proteins (called cohydrolase SPH-I here) was isolated from bovine, Gaucher human and control human spleens. All preparations exhibited a similar pattern of...
Free fatty acids in an animal model of reye's syndrome (1983)
Deshmukh, Devendra R., Deshmukh, Gayatri D., Shope, Thomas C., Radin, Norman S.
Recent studies have indicated that viral infections, aspirin treatment and hyperammonemia are associated with Reye's syndrome. It has also been reported that free fatty acids in serum and total...
Ford-Holevinski, Thomas S., Agranoff, Bernard W., Radin, Norman S.
An inexpensive video densitometer based on an Apple II microcomputer using commercially available components was developed. The device was used to quantitate charred lipid and colored spots on...
Radin, Norman S., Deshmukh, Gayatri D., Selvam, Ramasamy, Hospattankar, Ashok V.
A previously described method has been extended to various specific lipids of liver and brain. The basic method involves thin-layer chromatography followed by charring to reveal the bands. The...
An improved fluorometric leukocyte [beta]-glucosidase assay for Gaucher's disease (1981)
Daniels, Lydia B., Glew, Robert H., Diven, Warren F., Lee, Robert E., Radin, Norman S.
Three fluorometric leukocyte [beta]-glucosidase assays were compared for their ability to diagnose Gaucher's disease and identify carriers of the disorder: the acid [beta]-glucosidase assay of...
Berent, Susan L., Radin, Norman S.
The nature of the stimulatory action of the protein `coglucosidase' on gluco-cerebrosidase was investigated with the use of highly purified cofactor from bovine spleen, radioactive glucosyl ceramide...
[beta]-Glucosidase activator protein from bovine spleen ("coglucosidase") (1981)
Berent, Susan L., Radin, Norman S.
[beta]-Glucosidase-stimulating proteins ("co-[beta]-glucosidase") have been isolated from bovine spleen by acidification of homogenized spleen, heat denaturation, and chromatography with...
Selvam, Ramasamy, Radin, Norman S.
A method is described for the quantitative determination of lipids after separation by thin-layer chromatography: the lipid spots are visualized with a charring spray and the darkness of the spots is...
Daniels, Lydia B., Glew, Robert H., Radin, Norman S., Vunnam, Ranga R.
To date, enzymatic diagnosis of Gaucher's disease via a fluorometric assay procedure which utilizes 4-methylumbelliferyl-[beta]--glucopyranoside as a substrate has not been possible when liver serves...
Analogs of ceramide that inhibit glucocerebroside synthetase in mouse brain (1980)
Rao Vunnam, Ranga, Radin, Norman S.
In a search for potent inhibitors of glucocerebroside biosynthesis, we synthesized aromatic analogs of the enzyme's substrate, ceramide, many of which have not previously been described in the...
Hara, Atsushi, Radin, Norman S.
The ester groups of glycerophospholipids in tissue extracts can be cleaved in less than 10 min at room temperature if the lipids are extracted with hexane-isopropanol and the filtrate is treated with...
Vunnam, Ranga R., Radin, Norman S.
In order to increase the sensitivity of the assay for ceramide: UDPGlc glucosyltransferase, the enzyme that makes glucocerebroside, we synthesized a variety of ceramide homologues that might be...
Destruction and resynthesis of mouse [beta]-glucosidases (1979)
Hara, Atsushi, Radin, Norman S.
1. 1. Injection of a single dose of conduritol B epoxide into mice produced almost complete destruction of glucocerebrosidase ( glucohydrolase, EC 3.2.1.45) in liver, spleen, brain, and kidney within...
Enzymic effects of [beta]-glucosidase destruction in mice changes in glucuronidase levels (1979)
Hara, Atsushi, Radin, Norman S.
1. 1. The injection into mice of a single dose of conduritol B epoxide, a covalent inhibitor of glucosidases, quickly produced changes in tissue levels of [beta]--glucoronidase (EC 3.2.1.31). The...
Lipid extraction of tissues with a low-toxicity solvent (1978)
Hara, Atsushi, Radin, Norman S.
An improved method for extracting the lipids from tissues consists of the use of hexane:isopropanol, followed by a wash of the extract with aqueous sodium sulfate to remove nonlipid contaminants....
Immunogenicity of sulfatide (1977)
Zalc, B., Jacque, C., Radin, Norman S., Dupouey, P.
The present study deals with the immunogenicity of sulfatide. Despite its acidic character, we succeeded in raising high titer antibodies. The most effective immunization procedure involved repeated...
Effects of ceramide analogs on myelinating organ cultures (1976)
Benjamins, Joyce A., Fitch, John, Radin, Norman S.
Analogs of ceramide which inhibit galactocerebrosidase also demyelinate or inhibit myelination in organ cultures of rat cerebellum. The potency of the analogs in culture correlated with their...
Synthetic inhibitors of glucocerebroside [beta]-glucosidase (1975)
Hyun, Jung C., Misra, Radhey S., Greenblatt, David, Radin, Norman S.
The glucocerebrosidase of human placenta was studied with various potential inhibitors. Several compounds that resemble the lipoidal product of enzyme action, ceramide, proved to be excellent...
The inhibitor-sensitive sites of galactosyl ceramide galactosidase (1973)
Arora, Ramesh C., Lin, Yuh-Nan, Radin, Norman S.
A preparation of galactosyl ceramide galactosidase from rat brain has been tested with potential inhibitors which resemble the enzyme's substrate. The amide made from 2-hydroxydodecanoic acid and...
Ullman, M. David, Radin, Norman S.
Radioactive 2-hydroxystearic and cerebronic acids were converted to the coenzyme A thio esters, then tested for reactivity with -sphingosine. Microsomes from mouse brain were found to catalyze the...
Glial and neuronal localization of cerebroside-metabolizing enzymes (1972)
Radin, Norman S., Brenkert, Antoinette, Arora, Ramesh C., Sellinger, Otto Z., Flangas, Arthur L.
Glial and neuronal cell preparations were made from young rat cerebrum and assayed for 3 enzymes involved in sphingolipid metabolism. A galactosyltransferase which makes galactocerebroside, a primary...
Brenkert, Antoinette, Radin, Norman S.
Conditions were determined for assaying whole rat brain for the galactosyl- and glucosyltransferases which form cerebrosides from ceramide and a sugar nucleotide. The brain was homogenized in water,...
Cerebroside synthesis and hydrolysis in a neurological mutant mouse (MSD) (1972)
Brenkert, Antoinette, Arora, Ramesh C., Radin, Norman S., Meier, Hans, MacPike, Ann D.
Three enzymes related to myelin and ganglioside metabolism in the brains of normal and mutant (msd/Y) mice were compared through the ages 8-21 days old. No difference is seen in the...
The biosynthesis by brain microsomes of cerebrosides containing nonhydroxy fatty acids, (1970)
Morell, Pierre, Costantino-Ceccarini, Elvira, Radin, Norman S.
Incubation of microsomes from mouse brain with UDP-gal, a long-chain base, and 14C-fatty acyl-CoA resulted in formation of NFA-cerebroside labeled in the fatty acid residue. The rate of synthesis was...
Radin, Norman S., Hof, Liselotte, Bradley, Roy M., Brady, Roscoe O.
Four enzyme assays were carried out with brains from rats of age 4 days to about 320 days. The enzymes were acid hydrolases: lactosylceramide galactosidases, galactosylphenol galactosidase,...
Purification of cerebroside galactosidase from rat brain (1968)
Bowen, David M., Radin, Norman S.
A method is described for the preparation of a [beta]-galactosidase which acts on the brain lipid, ceramide galactoside. The enzyme is obtained from particles that sediment between 1000-13000 x g...
Properties of cerebroside galactosidase (1968)
Bowen, David M., Radin, Norman S.
Rat brain cerebrosidase was shown by centrifugal fractionation to be a lysosomal enzyme. The pH optimum was found to be 4.5. Taurocholate greatly stimulated its action. The addition of palmitic acid...
Biosynthesis of odd- and even-numbered cerebroside fatty acids: Evidence for two routes (1963)
Kajra, Amiya K., Radin, Norman S.
Peer Reviewed
Enzymic oxidation of cerebrosides: studies on Gaucher's disease (1962)
Agranoff, Bernard W., Radin, Norman S., Suomi, W.
Peer Reviewed
Lin, Yuh-Nan, Radin, Norman S.
Male and female mice were compared at two ages, 15 and 50 days, with respect to the activities of three galactosidases in kidney. No sex difference in enzyme activity was seen in the young mice, but...
Glucocerebrosidase: reconstitution of activity from macromolecular components. (Short Communication)
Ho, Mae Wan, O'Brien, John S., Radin, Norman S., Erickson, John S.
Glucocerebrosidase activity was reconstituted in vitro from a soluble glycoprotein factor (P) and a particle-bound factor (C). The physiological significance of the system is discussed.
Killing tumours by ceramide-induced apoptosis: a critique of available drugs.
Over 1000 research papers have described the production of programmed cell death (apoptosis) by interventions that elevate the cell content of ceramide (Cer). Other interventions, which lower...
Lin, Yuh-Nan, Radin, Norman S.
Male and female mice were compared at two ages, 15 and 50 days, with respect to the activities of three galactosidases in kidney. No sex difference in enzyme activity was seen in the young mice, but...
Glucocerebrosidase: reconstitution of activity from macromolecular components. (Short Communication)
Ho, Mae Wan, O'Brien, John S., Radin, Norman S., Erickson, John S.
Glucocerebrosidase activity was reconstituted in vitro from a soluble glycoprotein factor (P) and a particle-bound factor (C). The physiological significance of the system is discussed.
Killing tumours by ceramide-induced apoptosis: a critique of available drugs.
Over 1000 research papers have described the production of programmed cell death (apoptosis) by interventions that elevate the cell content of ceramide (Cer). Other interventions, which lower...
Drug design: hiding in full view
Compounds that can produce potent biological effects in cells encompass a variety of structural motifs. Many of these compounds share a structural feature that has rarely been noted. It is an allylic...