Scientific Commons: O. L. Gamborg

ETHYLENE PRODUCED BY PLANT CELLS IN SUSPENSION CULTURES, (2005)

The formation of ethylene by plants is increased by the application of growth regulators such as 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthaleneacetic acid (NAA). Plant cell suspension...

PREPARATION OF PREPHENIC ACID AND ITS CONVERSION TO PHENYLALANINE AND TYROSINE BY PLANT ENZYMES, (2005)

Gamborg,O. L., Simpson,F. J.

Enzymes from mung bean (Phaseolus aureus Roxb.) convert prephenic acid to phenylalanine and tyrosine. In addition to glutamate NADP was required for the production of tyrosine but no cofactor...

PLANT PROTOPLASTS FOR USE IN SOMATIC CELL HYBRIDIZATION, (1998)

Keller,W. A., Harvey,B., Gamborg,O. L., Miller,R. A., Eveleigh,D. E.

Somatic cell hybridization is a potentially useful technique for the introduction of genetic variability into plant species. One proposed method involves fusion of plant protoplasts (which is a cell...

Regeneration of Cassava Plants from Apical Meristems, (1998)

Kartha,K. K., Gamborg,O. L., Constabel,F., Shyluk,J. P.

Apical meristem culture offers a rapid, efficient method for vegetative propagation of plants and for eliminating systemic viral infections. Since the first demonstration that virus-free dahlia...

Bean Mesophyll Protoplasts: Production, Culture and Callus Formation, (1998)

Pelcher,L. E., Gamborg,O. L., Kao,K. N.

High yields of viable protoplasts were produced from leaf tissue of Phaseolus vulgaris L. var. Pinto. Consistent production of protoplasts depended on enzymatic liberation of the protoplasts near pH...

Regeneration of Pea ('Pisum sativum L'.) Plants from Shoot Apical Meristems, (1998)

Kartha,K. K., Gamborg,O. L., Constabel,F.

A procedure has been developed to obtain complete plants from meristems of three cultivars of Pisum sativum L. Benzyladenine (BA) alone or in combination with naphthaleneacetic acid (NAA) at molar...

Isolation and Properties of Deoxyribonucleic Acid from Protoplasts of Cell Suspension Cultures of Ammi visnaga and Carrot (Daucus carota) 1

Ohyama, K., Gamborg, O. L., Miller, R. A.

A procedure is described for the isolation of native DNA from protoplasts of ammi (Ammi visnaga) and carrot (Daucus carota) cells. Protoplasts were produced from 40 grams of fresh cells by enzyme...

Ethylene Production by Plant Cell Cultures: Variations in Production during Growing Cycle and in Different Plant Species 1

Larue, T. A. G., Gamborg, O. L.

Suspension cultures of Rosa sp., soybean (Glycine max L.), wheat (Triticum monococcum L.), sweet clover (Melilotus alba Desc.), Haplopappus gracilis Nutt., and rue (Ruta graveolens) produced...

Ethylene Production by Plant Cell Cultures: The Effect of Auxins, Abscisic Acid, and Kinetin on Ethylene Production in Suspension Cultures of Rose and Ruta Cells 1

Gamborg, O. L., Larue, T. A. G.

Cell suspension cultures of Ruta graveolens (rue) and Rosa sp. produce ethylene. Both cultures grow at a high rate in hormone-free media. The rose cells are undifferentiated while the Ruta cells...