Olivier Denis

Epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) among residents of nursing homes in Belgium (2009)

Denis, Olivier, Jans, Bea, Deplano, Ariane, Nonhoff, Claire, De Ryck, Raf, Suetens, Carl, ...

Objectives A national survey was conducted to determine the prevalence, risk factors and molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) carriage among nursing home (NH)...

Antimicrobial resistance of old and recent staphylococcus aureus (2008)

Nemati, Mostafa, Hermans, Katleen, Lipinska, Urszula, Denis, Olivier, Deplano, Ariane, Struelens, Marc, ...

The susceptibilities of 12 antimicrobial agents for two collections of Staphylococcus aureus, isolated in the 1970s and in 2006 from poultry, were determined. For eight antibiotics, the percentage of...

Evaluation of disc diffusion methods and Vitek 2 automated system for testing susceptibility to mupirocin in Staphylococcus aureus (2008)

Malaviolle, Xavier, Nonhoff, Claire, Denis, Olivier, Rottiers, Sylvianne, Struelens, Marc J.

Objectives To compare the performance of the automated Vitek 2 system, the disc diffusion method and a home-made mupirocin screen agar (MSA) to detect mupirocin resistance in methicillin-resistant...

Comparison of antimicrobial resistance in old and recent Staphylococcus aureus isolates from broilers (2008)

Nemati, Mostafa, Hermans, Katleen, Lipinska, Urszula, Denis, Olivier, Deplano, Ariane, Struelens, Marc, ...

Travel-acquired salmonellosis due to Salmonella Kentucky resistant to ciprofloxacin, ceftriaxone and co-trimoxazole and associated with treatment failure (2007)

Collard, Jean-Marc, Place, Sammy, Denis, Olivier, Rodriguez-Villalobos, Hector, Vrints, Martine, Weill, François-Xavier, ...

Travel-acquired salmonellosis due to Salmonella Kentucky resistant to ciprofloxacin, ceftriaxone and co-trimoxazole and associated with treatment failure (2007)

Collard, Jean-Marc, Place, Sammy, Denis, Olivier, Rodriguez-Villalobos, Hector, Vrints, Martine, Weill, François-Xavier, ...

Rapid molecular detection of methicillin-resistant Staphylococcus aureus: a cost-effective tool for infection control in critical care? (2006)

Struelens, Marc J, Denis, Olivier

Abstract Control strategies for methicillin-resistant Staphylococcus aureus (MRSA) in critical care remain debated. Timely detection of MRSA carriers is crucial to an effective isolation policy. In...

Molecular epidemiology of resistance to macrolides-lincosamides-streptogramins in methicillin-resistant Staphylococcusaureus (MRSA) causing bloodstream infections in patients admitted to Belgian hospitals (2002)

Denis, Olivier, Magdalena, Juana, Deplano, Ariane, Nonhoff, Claire, Hendrickx, Erik, Struelens, Marc J.

Molecular epidemiology of resistance to macrolides-lincosamides-streptogramins in methicillin-resistant Staphylococcusaureus (MRSA) causing bloodstream infections in patients admitted to Belgian hospitals (2002)

Denis, Olivier, Magdalena, Juana, Deplano, Ariane, Nonhoff, Claire, Hendrickx, Erik, Struelens, Marc J.

Emergence of vancomycin-intermediate Staphylococcus aureus in a Belgian hospital: microbiological and clinical features (2002)

Denis, Olivier, Nonhoff, Claire, Byl, Baudouin, Knoop, Christiane, Bobin-Dubreux, Sophie, Struelens, Marc J.

In 1999, all clinical Staphylococcus aureus isolates from patients admitted to a Belgian University hospital were tested for decreased vancomycin susceptibility. Three vancomycin-intermediate...

Molecular epidemiology of resistance to macrolides-lincosamides-streptogramins in methicillin-resistant Staphylococcusaureus (MRSA) causing bloodstream infections in patients admitted to Belgian hospitals (2002)

Denis, Olivier, Magdalena, Juana, Deplano, Ariane, Nonhoff, Claire, Hendrickx, Erik, Struelens, Marc J.

Characterization of the culture filtrate-specific cytotoxic T lymphocyte response induced by bacillus Calmette-Guerin vaccination in H-2b mice (1999)

Denis, Olivier, Huygen, Kris

Although CD8+ T cells are supposed to play an important role in protective immunity to mycobacteria, cytotoxic T lymphocyte (CTL) responses in this infection remain poorly characterized. We...

The perinatal presence of antigen (p-azophenylarsonate) or anti-{micro} antibodies lead to the loss of the recurrent idiotype (CRIA) in A/J mice (1995)

Ryelandt, Marion, De Wit, Dominique, Baz, Adriana, Vansanten, Georgette, Denis, Olivier, Huetz, François, ...

The immune response of A/J mice against p-azophenylarsonate (Ars)—keyhole limpet hemocyanln(KLH) is characterized by the dominance, late in primary and during the secondary, of a recurrent...

Resting B cells can act as antigen presenting cells in vivo andinduce antibody responses (1993)

Denis, Olivier, Latinne, Dominique, Nisol, Françoise, Bazin, Hervé, Eichmann, K.

Although it is well established that B lymphocytes are able to present antigen in vitro, the ability of small resting B cells to act as antigen presenting cells in vivo remains controversial. In this...

Cloning of the Gene Encoding a 22-Kilodalton Cell Surface Antigen of Mycobacterium bovis BCG and Analysis of Its Potential for DNA Vaccination against Tuberculosis

Lefèvre, Philippe, Denis, Olivier, De Wit, Lucas, Tanghe, Audrey, Vandenbussche, Paul, Content, Jean, ...

Using spleen cells from mice vaccinated with live Mycobacterium bovis BCG, we previously generated three monoclonal antibodies reactive against a 22-kDa protein present in mycobacterial culture...

Lack of Protection in Mice and Necrotizing Bronchointerstitial Pneumonia with Bronchiolitis in Guinea Pigs Immunized with Vaccines Directed against the hsp60 Molecule of Mycobacterium tuberculosis

Turner, Oliver C., Roberts, Alan D., Frank, Anthony A., Phalen, Susan W., McMurray, David M., Content, Jean, ...

In this study, the hsp60 and hsp70 heat shock protein antigens of Mycobacterium tuberculosis were tested as potential vaccine candidates, using purified recombinant protein antigens or antigens...

Improved Immunogenicity and Protective Efficacy of a Tuberculosis DNA Vaccine Encoding Ag85 by Protein Boosting

Tanghe, Audrey, D'Souza, Sushila, Rosseels, Valérie, Denis, Olivier, Ottenhoff, Thomas H. M., Dalemans, Wilfried, ...

C57BL/6 mice were vaccinated with plasmid DNA encoding Ag85 from Mycobacterium tuberculosis, with Ag85 protein in adjuvant, or with a combined DNA prime-protein boost regimen. While DNA immunization,...

Tuberculosis DNA Vaccine Encoding Ag85A Is Immunogenic and Protective When Administered by Intramuscular Needle Injection but Not by Epidermal Gene Gun Bombardment

Tanghe, Audrey, Denis, Olivier, Lambrecht, Bénédicte, Motte, Vinciane, Van Den Berg, Thierry, Huygen, Kris

Immunogenicity and protective efficacy of a DNA vaccine encoding Ag85A from Mycobacterium tuberculosis were compared in BALB/c and C57BL (B6 and B10) mice immunized by intramuscular (i.m.) needle...

Vaccination with Plasmid DNA Encoding Mycobacterial Antigen 85A Stimulates a CD4+ and CD8+ T-Cell Epitopic Repertoire Broader than That Stimulated by Mycobacterium tuberculosis H37Rv Infection

Denis, Olivier, Tanghe, Audrey, Palfliet, Kamiel, Jurion, Fabienne, Van Den Berg, Thierry-P., Vanonckelen, Albert, ...

Vaccination of mice with plasmid DNA carrying the gene for the major secreted mycobacterial antigen 85A (Ag85A) from Mycobacterium tuberculosis is a powerful technique for generating robust specific...

National Surveillance of Methicillin-Resistant Staphylococcus aureus in Belgian Hospitals Indicates Rapid Diversification of Epidemic Clones

Denis, Olivier, Deplano, Ariane, Nonhoff, Claire, De Ryck, Raf, De Mendonça, Ricardo, Rottiers, Sylvianne, ...

Methicillin-resistant Staphylococcus aureus (MRSA) strains (n = 455) collected in 2001 from 100 Belgian hospitals were characterized by molecular typing and by resistance gene distribution to...

Mycobacterium tuberculosis with Disruption in Genes Encoding the Phosphate Binding Proteins PstS1 and PstS2 Is Deficient in Phosphate Uptake and Demonstrates Reduced In Vivo Virulence

Peirs, Priska, Lefèvre, Philippe, Boarbi, Samira, Wang, Xiao-Ming, Denis, Olivier, Braibant, Martine, ...

By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding...

Rapid molecular detection of methicillin-resistant Staphylococcus aureus: a cost-effective tool for infection control in critical care?

Struelens, Marc J, Denis, Olivier

Control strategies for methicillin-resistant Staphylococcus aureus (MRSA) in critical care remain debated. Timely detection of MRSA carriers is crucial to an effective isolation policy. In this...

In Vitro Activities of Ceftobiprole, Tigecycline, Daptomycin, and 19 Other Antimicrobials against Methicillin-Resistant Staphylococcus aureus Strains from a National Survey of Belgian Hospitals

Denis, Olivier, Deplano, Ariane, Nonhoff, Claire, Hallin, Marie, De Ryck, Raf, Vanhoof, Raymond, ...

The in vitro activities of 22 antimicrobial agents, including ceftobiprole, daptomycin, and tigecycline, against 511 methicillin-resistant Staphylococcus aureus (MRSA) isolates from 112 Belgian...

Cloning of the Gene Encoding a 22-Kilodalton Cell Surface Antigen of Mycobacterium bovis BCG and Analysis of Its Potential for DNA Vaccination against Tuberculosis

Lefèvre, Philippe, Denis, Olivier, De Wit, Lucas, Tanghe, Audrey, Vandenbussche, Paul, Content, Jean, ...

Using spleen cells from mice vaccinated with live Mycobacterium bovis BCG, we previously generated three monoclonal antibodies reactive against a 22-kDa protein present in mycobacterial culture...

Lack of Protection in Mice and Necrotizing Bronchointerstitial Pneumonia with Bronchiolitis in Guinea Pigs Immunized with Vaccines Directed against the hsp60 Molecule of Mycobacterium tuberculosis

Turner, Oliver C., Roberts, Alan D., Frank, Anthony A., Phalen, Susan W., McMurray, David M., Content, Jean, ...

In this study, the hsp60 and hsp70 heat shock protein antigens of Mycobacterium tuberculosis were tested as potential vaccine candidates, using purified recombinant protein antigens or antigens...

Improved Immunogenicity and Protective Efficacy of a Tuberculosis DNA Vaccine Encoding Ag85 by Protein Boosting

Tanghe, Audrey, D'Souza, Sushila, Rosseels, Valérie, Denis, Olivier, Ottenhoff, Thomas H. M., Dalemans, Wilfried, ...

C57BL/6 mice were vaccinated with plasmid DNA encoding Ag85 from Mycobacterium tuberculosis, with Ag85 protein in adjuvant, or with a combined DNA prime-protein boost regimen. While DNA immunization,...

Tuberculosis DNA Vaccine Encoding Ag85A Is Immunogenic and Protective When Administered by Intramuscular Needle Injection but Not by Epidermal Gene Gun Bombardment

Tanghe, Audrey, Denis, Olivier, Lambrecht, Bénédicte, Motte, Vinciane, Van Den Berg, Thierry, Huygen, Kris

Immunogenicity and protective efficacy of a DNA vaccine encoding Ag85A from Mycobacterium tuberculosis were compared in BALB/c and C57BL (B6 and B10) mice immunized by intramuscular (i.m.) needle...

Vaccination with Plasmid DNA Encoding Mycobacterial Antigen 85A Stimulates a CD4+ and CD8+ T-Cell Epitopic Repertoire Broader than That Stimulated by Mycobacterium tuberculosis H37Rv Infection

Denis, Olivier, Tanghe, Audrey, Palfliet, Kamiel, Jurion, Fabienne, Van Den Berg, Thierry-P., Vanonckelen, Albert, ...

Vaccination of mice with plasmid DNA carrying the gene for the major secreted mycobacterial antigen 85A (Ag85A) from Mycobacterium tuberculosis is a powerful technique for generating robust specific...

National Surveillance of Methicillin-Resistant Staphylococcus aureus in Belgian Hospitals Indicates Rapid Diversification of Epidemic Clones

Denis, Olivier, Deplano, Ariane, Nonhoff, Claire, De Ryck, Raf, De Mendonça, Ricardo, Rottiers, Sylvianne, ...

Methicillin-resistant Staphylococcus aureus (MRSA) strains (n = 455) collected in 2001 from 100 Belgian hospitals were characterized by molecular typing and by resistance gene distribution to...

Mycobacterium tuberculosis with Disruption in Genes Encoding the Phosphate Binding Proteins PstS1 and PstS2 Is Deficient in Phosphate Uptake and Demonstrates Reduced In Vivo Virulence

Peirs, Priska, Lefèvre, Philippe, Boarbi, Samira, Wang, Xiao-Ming, Denis, Olivier, Braibant, Martine, ...

By measuring phosphate uptake by Mycobacterium tuberculosis strains with the pstS1 and pstS2 genes genetically inactivated, we showed that these pstS genes encode high-affinity phosphate binding...

In Vitro Activities of Ceftobiprole, Tigecycline, Daptomycin, and 19 Other Antimicrobials against Methicillin-Resistant Staphylococcus aureus Strains from a National Survey of Belgian Hospitals

Denis, Olivier, Deplano, Ariane, Nonhoff, Claire, Hallin, Marie, De Ryck, Raf, Vanhoof, Raymond, ...

The in vitro activities of 22 antimicrobial agents, including ceftobiprole, daptomycin, and tigecycline, against 511 methicillin-resistant Staphylococcus aureus (MRSA) isolates from 112 Belgian...

Rapid molecular detection of methicillin-resistant Staphylococcus aureus: a cost-effective tool for infection control in critical care?

Struelens, Marc J, Denis, Olivier

Control strategies for methicillin-resistant Staphylococcus aureus (MRSA) in critical care remain debated. Timely detection of MRSA carriers is crucial to an effective isolation policy. In this...

Immunogenicity and protective efficacy of tuberculosis DNA vaccines combining mycolyl-transferase Ag85A and phosphate transport receptor PstS-3

Romano, Marta, Roupie, Virginie, Wang, Xiao M, Denis, Olivier, Jurion, Fabienne, Adnet, Pierre-Yves, ...

DNA vaccines encoding the 32 000 MW mycolyl-transferase Ag85A and the 40 000 MW phosphate-binding protein PstS-3 can elicit protective immune responses against experimental infection with...

Controlled Evaluation of the IDI-MRSA Assay for Detection of Colonization by Methicillin-Resistant Staphylococcus aureus in Diverse Mucocutaneous Specimens▿

De San, Nour, Denis, Olivier, Gasasira, Marie-Fabrice, De Mendonça, Ricardo, Nonhoff, Claire, Struelens, Marc J.

Rapid and reliable detection of methicillin-resistant Staphylococcus aureus (MRSA) carriers is crucial for the effective control of MRSA transmission in healthcare facilities. The aim of this study...

Chronic intranasal administration of mould spores or extracts to unsensitized mice leads to lung allergic inflammation, hyper-reactivity and remodelling

Denis, Olivier, Van Den Brûle, Sybille, Heymans, Julie, Havaux, Xavier, Rochard, Christelle, Huaux, François, ...

Allergic asthma is a serious multifaceted disease characterized by eosinophil-rich airway inflammation, airway hyperreactivity and airway wall modifications known as remodelling. We previously...

Evaluation of New Vitek 2 Card and Disk Diffusion Method for Determining Susceptibility of Staphylococcus aureus to Oxacillin▿

Roisin, Sandrine, Nonhoff, Claire, Denis, Olivier, Struelens, Marc J.

Detection of methicillin resistance in Staphylococcus aureus is a challenge, especially low-level resistance, which is often misdiagnosed. The aim of this study was to compare the diagnostic...

Antimicrobial Resistance of Old and Recent Staphylococcus aureus Isolates from Poultry: First Detection of Livestock-Associated Methicillin-Resistant Strain ST398 ▿

Nemati, Mostafa, Hermans, Katleen, Lipinska, Urszula, Denis, Olivier, Deplano, Ariane, Struelens, Marc, ...

The susceptibilities of 12 antimicrobial agents for two collections of Staphylococcus aureus, isolated in the 1970s and in 2006 from poultry, were determined. For eight antibiotics, the percentage of...

Evaluation of New Vitek 2 Card and Disk Diffusion Method for Determining Susceptibility of Staphylococcus aureus to Oxacillin

Roisin, Sandrine, Nonhoff, Claire, Denis, Olivier, Struelens, Marc J.

Intracellular Activity of Antibiotics in a Model of Human THP-1 Macrophages Infected by a Staphylococcus aureus Small-Colony Variant Strain Isolated from a Cystic Fibrosis Patient: Pharmacodynamic Evaluation and Comparison with Isogenic Normal-Phenotype and Revertant Strains▿ †

Nguyen, Hoang Anh, Denis, Olivier, Vergison, Anne, Theunis, Anne, Tulkens, Paul M., Struelens, Marc J., ...

Small-colony variant (SCV) strains of Staphylococcus aureus show reduced antibiotic susceptibility and intracellular persistence, potentially explaining therapeutic failures. The activities of...

Intracellular Activity of Antibiotics in a Model of Human THP-1 Macrophages Infected by a Staphylococcus aureus Small-Colony Variant Strain Isolated from a Cystic Fibrosis Patient: Study of Antibiotic Combinations▿ †

Nguyen, Hoang Anh, Denis, Olivier, Vergison, Anne, Tulkens, Paul M., Struelens, Marc J., Van Bambeke, Françoise

In a companion paper (H. A. Nguyen et al., Antimicrob. Agents Chemother. 53:1434-1442, 2009), we showed that vancomycin, oxacillin, fusidic acid, clindamycin, linezolid, and daptomycin are poorly...

Methicillin-Resistant Staphylococcus aureus ST398 in Swine Farm Personnel, Belgium

Denis, Olivier, Suetens, Carl, Hallin, Marie, Catry, Boudewijn, Ramboer, Ilse, Dispas, Marc, ...

We assessed methicillin-resistant Staphylococcus aureus (MRSA) in persons on 49 swine farms in Belgium. Surveys showed that 48 (37.8%) persons carried MRSA ST398 and 1 (0.8%) had concurrent skin...