Oscar Puig

The U1 snRNP-associated factor Luc7p affects 5' splice site selection in yeast and human (2007)

Puig, Oscar, Bragado-Nilsson, Elisabeth, Koski, Terhi, Séraphin, Bertrand

yLuc7p is an essential subunit of the yeast U1 snRNP and contains two putative zinc fingers. Using RNA–protein cross-linking and directed site-specific proteolysis (DSSP), we have established that...

IRES-mediated functional coupling of transcription and translation amplifies insulin receptor feedback (2007)

Marr, Michael T., D’Alessio, Joseph A., Puig, Oscar, Tjian, Robert

It is generally accepted that the growth rate of an organism is modulated by the availability of nutrients. One common mechanism to control cellular growth is through the global down-regulation of...

Transcriptional feedback control of insulin receptor by dFOXO/FOXO1 (2005)

Puig, Oscar, Tjian, Robert

The insulin signaling pathway, which is conserved in evolution from flies to humans, evolved to allow a fast response to changes in nutrient availability while keeping glucose concentration constant...

Control of cell number by Drosophila FOXO: downstream and feedback regulation of the insulin receptor pathway (2003)

Puig, Oscar, Marr, Michael T., Ruhf, M. Laure, Tjian, Robert

The Drosophila insulin receptor (dInR) regulates cell growth and proliferation through the dPI3K/dAkt pathway, which is conserved in metazoan organisms. Here we report the identification and...

Control of cell number by Drosophila FOXO: downstream and feedback regulation of the insulin receptor pathway (2003)

Puig, Oscar, Marr, Michael T., Ruhf, M. Laure, Tjian, Robert

The Drosophila insulin receptor (dInR) regulates cell growth and proliferation through the dPI3K/dAkt pathway, which is conserved in metazoan organisms. Here we report the identification and...

The splicing regulator TIA-1 interacts with U1-C to promote U1 snRNP recruitment to 5′ splice sites

Förch, Patrik, Puig, Oscar, Martínez, Concepción, Séraphin, Bertrand, Valcárcel, Juan

The U1 small nuclear ribonucleoprotein (U1 snRNP) binds to the pre-mRNA 5′ splice site (ss) at early stages of spliceosome assembly. Recruitment of U1 to a class of weak 5′ ss is promoted by...

Control of cell number by Drosophila FOXO: downstream and feedback regulation of the insulin receptor pathway

Puig, Oscar, Marr, Michael T., Ruhf, M. Laure, Tjian, Robert

The Drosophila insulin receptor (dInR) regulates cell growth and proliferation through the dPI3K/dAkt pathway, which is conserved in metazoan organisms. Here we report the identification and...

Interaction of the U1 snRNP with nonconserved intronic sequences affects 5′ splice site selection

Puig, Oscar, Gottschalk, Alexander, Fabrizio, Patrizia, Séraphin, Bertrand

Intron definition and splice site selection occur at an early stage during assembly of the spliceosome, the complex mediating pre-mRNA splicing. Association of U1 snRNP with the pre-mRNA is required...

Transcriptional feedback control of insulin receptor by dFOXO/FOXO1

Puig, Oscar, Tjian, Robert

The insulin signaling pathway, which is conserved in evolution from flies to humans, evolved to allow a fast response to changes in nutrient availability while keeping glucose concentration constant...

The splicing regulator TIA-1 interacts with U1-C to promote U1 snRNP recruitment to 5′ splice sites

Förch, Patrik, Puig, Oscar, Martínez, Concepción, Séraphin, Bertrand, Valcárcel, Juan

The U1 small nuclear ribonucleoprotein (U1 snRNP) binds to the pre-mRNA 5′ splice site (ss) at early stages of spliceosome assembly. Recruitment of U1 to a class of weak 5′ ss is promoted by...

Control of cell number by Drosophila FOXO: downstream and feedback regulation of the insulin receptor pathway

Puig, Oscar, Marr, Michael T., Ruhf, M. Laure, Tjian, Robert

The Drosophila insulin receptor (dInR) regulates cell growth and proliferation through the dPI3K/dAkt pathway, which is conserved in metazoan organisms. Here we report the identification and...

Interaction of the U1 snRNP with nonconserved intronic sequences affects 5′ splice site selection

Puig, Oscar, Gottschalk, Alexander, Fabrizio, Patrizia, Séraphin, Bertrand

Intron definition and splice site selection occur at an early stage during assembly of the spliceosome, the complex mediating pre-mRNA splicing. Association of U1 snRNP with the pre-mRNA is required...

Transcriptional feedback control of insulin receptor by dFOXO/FOXO1

Puig, Oscar, Tjian, Robert

The insulin signaling pathway, which is conserved in evolution from flies to humans, evolved to allow a fast response to changes in nutrient availability while keeping glucose concentration constant...

IRES-mediated functional coupling of transcription and translation amplifies insulin receptor feedback

Marr, Michael T., D’Alessio, Joseph A., Puig, Oscar, Tjian, Robert

It is generally accepted that the growth rate of an organism is modulated by the availability of nutrients. One common mechanism to control cellular growth is through the global down-regulation of...

The U1 snRNP-associated factor Luc7p affects 5′ splice site selection in yeast and human

Puig, Oscar, Bragado-Nilsson, Elisabeth, Koski, Terhi, Séraphin, Bertrand

yLuc7p is an essential subunit of the yeast U1 snRNP and contains two putative zinc fingers. Using RNA–protein cross-linking and directed site-specific proteolysis (DSSP), we have established that...

Foxo and Fos regulate the decision between cell death and survival in response to UV irradiation

Luo, Xi, Puig, Oscar, Hyun, Joogyung, Bohmann, Dirk, Jasper, Heinrich

Cells damaged by environmental insults have to be repaired or eliminated to ensure tissue homeostasis in metazoans. Recent studies suggest that the balance between cell survival signals and...

FOXO-regulated transcription restricts overgrowth of Tsc mutant organs

Harvey, Kieran F., Mattila, Jaakko, Sofer, Avi, Bennett, F. Christian, Ramsey, Matthew R., Ellisen, Leif W., ...

FOXO is thought to function as a repressor of growth that is, in turn, inhibited by insulin signaling. However, inactivating mutations in Drosophila melanogaster FOXO result in viable flies of normal...

RNAi screening for kinases and phosphatases identifies FoxO regulators

Mattila, Jaakko, Kallijärvi, Jukka, Puig, Oscar

Forkhead box class O (FoxO) transcription factors are key regulators of growth, metabolism, life span, and stress resistance. FoxOs integrate signals from different pathways and guide the cellular...