Possible Ion Channel Model in Crystals of Leu-Zervamicin, (9999)
Karle, I. L., Flippen-Anderson, J. L., Agarwalla, S., Balaram, P.
The best-studied members of this class are the alamethicins for which a crystal structure has been reported. The conformation of the peptide molecule was established to be a bent helix; however, the...
An internally quenched fluorescent substrate for collagenase (2008)
A synthetic collagenase substrate containing the internal peptide sequence—Gly-Gly-Pro-Leu-Gly-Pro-Pro-Gly- Pro—has been synthesized, with an N-terminus 4-((4-(dimethylamino)phenyl)azo)-benzoyl...
An internally quenched fluorescent substrate for collagenase (2008)
A synthetic collagenase substrate containing the internal peptide sequence—Gly-Gly-Pro-Leu-Gly-Pro-Pro-Gly- Pro—has been synthesized, with an N-terminus 4-((4-(dimethylamino)phenyl)azo)-benzoyl...
Tendulkar, SR, Saikumari, YK, Patel, V, Raghotama, S, Munshi, TK, Balaram, P, ...
Aims: Isolation of bacterial antagonist for use in the biological control of phytopathogenic fungi like rice blast fungus, Magnaporthe grisea, and to further purify and characterize the antifungal...
Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii (2007)
Gayathri, P, Banerjee, Mousumi, Vijayalakshmi, A, Azeez, Shamina, Balaram, Hemalatha, Balaram, P, ...
The crystal structure of a recombinant triosephosphate isomerase (TIM) from the archaeabacterium Methanocaldococcus jannaschii has been determined at a resolution of 2.3 \AA using X-ray diffraction...
Current Science: Some early history (2007)
1932, the year in which Current Science was born, saw the world in the midst of transition. Europe, then the centre of science had the First World War well behind it and was purposefully heading...
Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii (2007)
Gayathri, P, Banerjee, Mousumi, Vijayalakshmi, A, Azeez, Shamina, Balaram, Hemalatha, Balaram, P, ...
The crystal structure of a recombinant triosephosphate isomerase (TIM) from the archaeabacterium Methanocaldococcus jannaschii has been determined at a resolution of 2.3 \AA using X-ray diffraction...
Current Science: Some early history (2007)
1932, the year in which Current Science was born, saw the world in the midst of transition. Europe, then the centre of science had the First World War well behind it and was purposefully heading...
Sabareesh, V., Gowd, Hanumae K, Ramasamy, P, Sudarslal, S, Krishnan, KS, Sikda, SK, ...
Distinctly different effects of two closely related contryphans have been demonstrated on voltage-activated $Ca^{2+}$ channels. The peptides Lo959 and Am975 were isolated from Conus loroisii, a...
Sabareesh, V., Gowd, Hanumae K, Ramasamy, P, Sudarslal, S, Krishnan, KS, Sikda, SK, ...
Distinctly different effects of two closely related contryphans have been demonstrated on voltage-activated $Ca^{2+}$ channels. The peptides Lo959 and Am975 were isolated from Conus loroisii, a...
Tryptophan-containing peptide helices: interactions involving the indole side chain (2005)
Mahalakshmi, R, Sengupta, A, Raghothama, S, Shamala, N, Balaram, P
TTwo designed peptide sequences containing Trp residues at positions i and i + 5 ( Boc- Leu- Trp- Val- Ala- Aib- Leu- Trp- Val- OMe, 1) as well as i and i + 6 ( Boc- Leu- Trp- Val- Aib- Ala- Aib-...
The annual budget is presented invariably on 28 February, a date that coincides with National Science Day, which celebrates C. V. Raman's historic discovery of the effect which bears his name....
Sengupta, A, Mahalakshmi, R, Shamala, N, Balaram, P
The crystal structures of the peptides, Boc-Leu-Trp-Val-OMe (1), Ac-Leu-Trp-Val-OMe (2a and 2b), Boc-Leu-Phe-Val-OMe (3), Ac-Leu-Phe-Val-OMe (4), and Boc-Ala-Aib-Leu-Trp-Val-OMe (5) have been...
The annual budget is presented invariably on 28 February, a date that coincides with National Science Day, which celebrates C. V. Raman's historic discovery of the effect which bears his name....
Tryptophan-containing peptide helices: interactions involving the indole side chain (2005)
Mahalakshmi, R, Sengupta, A, Raghothama, S, Shamala, N, Balaram, P
TTwo designed peptide sequences containing Trp residues at positions i and i + 5 ( Boc- Leu- Trp- Val- Ala- Aib- Leu- Trp- Val- OMe, 1) as well as i and i + 6 ( Boc- Leu- Trp- Val- Aib- Ala- Aib-...
Sengupta, A, Mahalakshmi, R, Shamala, N, Balaram, P
The crystal structures of the peptides, Boc-Leu-Trp-Val-OMe (1), Ac-Leu-Trp-Val-OMe (2a and 2b), Boc-Leu-Phe-Val-OMe (3), Ac-Leu-Phe-Val-OMe (4), and Boc-Ala-Aib-Leu-Trp-Val-OMe (5) have been...
Roy, Rituparna S, Karle, Isabella L, Raghothama, S, Balaram, P
Conformational studies on the synthetic 11-aa peptide t-butoxycarbonyl (Boc)-Val-Ala-Phe-$\alpha$-aminoisobutyric acid (Aib)-(R)-$\beta^3$- homovaline ($\beta$Val)-(S)-$\beta^3$-homophenylalanine...
Eaazhisai, K, Balaram, H, Balaram, P, Murthy, MRN
The enzymatic reaction of triosephosphate isomerase (TIM) is controlled by the movement of a loop (loop6, residues 166–176). Crystal structures of TIMs from a variety of sources have revealed that...
Anil Kumar Lala, Professor of Chemistry at the Indian Institute of Technology (IIT) Bombay and a member of the Editorial Board of Protein Science, died on July 17, 2004, following a stroke that he...
Anil Kumar Lala (1950–2004) (2004)
Anil Kumar Lala, Professor of Chemistry at the Indian Institute of Technology (IIT), Bombay died on 17 July 2004, following a stroke that he suffered three weeks earlier. Born on 13 January 1950,...
Conformational properties of hybrid peptides containing a- and w-amino acids (2004)
This review briefly surveys the conformational properties of guest w-amino acid residues when incorporated into host a-peptide sequences. The results presented focus primarily on the use of b- and...
Conformational properties of hybrid peptides containing a- and w-amino acids (2004)
This review briefly surveys the conformational properties of guest w-amino acid residues when incorporated into host a-peptide sequences. The results presented focus primarily on the use of b- and...
Anil Kumar Lala (1950–2004) (2004)
Anil Kumar Lala, Professor of Chemistry at the Indian Institute of Technology (IIT), Bombay died on 17 July 2004, following a stroke that he suffered three weeks earlier. Born on 13 January 1950,...
Datta, Saumen, Rathore, RNS, Vijayalakshmi, S, Vasudev, Prema G, Rao, Balaji R, Balaram, P, ...
A pentapeptide, $Boc-Leu-Ac_8c-Ala-Leu-Ac_8c-OMe 1$, an octapeptide, $Boc-Leu-Ac_8c-Ala-Leu-Ac_8c-Ala-Leu-Ac_8c-OMe 2$ and a tripeptide, $Boc-Aib-Ac_8c-Aib-OMe 3$ containing the...
Roy, Rituparna S, Karle, Isabella L, Raghothama, S, Balaram, P
Conformational studies on the synthetic 11-aa peptide t-butoxycarbonyl (Boc)-Val-Ala-Phe-$\alpha$-aminoisobutyric acid (Aib)-(R)-$\beta^3$- homovaline ($\beta$Val)-(S)-$\beta^3$-homophenylalanine...
Eaazhisai, K, Balaram, H, Balaram, P, Murthy, MRN
The enzymatic reaction of triosephosphate isomerase (TIM) is controlled by the movement of a loop (loop6, residues 166–176). Crystal structures of TIMs from a variety of sources have revealed that...
Anil Kumar Lala, Professor of Chemistry at the Indian Institute of Technology (IIT) Bombay and a member of the Editorial Board of Protein Science, died on July 17, 2004, following a stroke that he...
Datta, Saumen, Rathore, RNS, Vijayalakshmi, S, Vasudev, Prema G, Rao, Balaji R, Balaram, P, ...
A pentapeptide, $Boc-Leu-Ac_8c-Ala-Leu-Ac_8c-OMe 1$, an octapeptide, $Boc-Leu-Ac_8c-Ala-Leu-Ac_8c-Ala-Leu-Ac_8c-OMe 2$ and a tripeptide, $Boc-Aib-Ac_8c-Aib-OMe 3$ containing the...
Non-protein amino acids in peptide design (2003)
Aravinda, S, Shamala, N, Roy, Rituparna S, Balaram, P
An overview of the use of non-protein amino acids in the design of conformationally well-defined peptides, based on work from the author’s laboratory, is discussed. The crystal structures of...
Sodium channel modulating activity in a delta-conotoxin from an Indian marine snail (2003)
Sudarslal, S, Majumdar, Sriparna, Ramasamy, P, Dhawan, Ritu, Pal, Prajna P, Ramaswami, Mani, ...
A 26 residue peptide (Am 2766) with the sequence CKQAGESCDIFSQNCCVG-TCAFICIE-NH2 has been isolated and purified from the venom of the molluscivorous snail, Conus amadis, collected off the...
Ananda, K, Aravinda, S, Vasudev, Prema G, Raja, Muruga Poopathi K, Sivaramakrishnan, H, Nagarajan, K, ...
Gabapentin (1-(aminomethyl)cycloheaxaneacetic acid; Gpn) is a widely used anti-epileptic drug. The target site of action of Gpn remains controversial. Gpn can exist in two isomeric chair forms. The...
Registering alpha-Helices and beta-Strands Using Backbone C-H…O Interactions (2003)
Singh, Kumar S, Babu, Madan M, Balaram, P
The possible occurrence of a novel helix terminating structural motif in proteins involving a stabilizing short C-H...O interaction has been examined using a dataset of 634 non-homologous protein...
Non-protein amino acids in peptide design (2003)
Aravinda, S, Shamala, N, Roy, Rituparna S, Balaram, P
An overview of the use of non-protein amino acids in the design of conformationally well-defined peptides, based on work from the author’s laboratory, is discussed. The crystal structures of...
Sodium channel modulating activity in a delta-conotoxin from an Indian marine snail (2003)
Sudarslal, S, Majumdar, Sriparna, Ramasamy, P, Dhawan, Ritu, Pal, Prajna P, Ramaswami, Mani, ...
A 26 residue peptide (Am 2766) with the sequence CKQAGESCDIFSQNCCVG-TCAFICIE-NH2 has been isolated and purified from the venom of the molluscivorous snail, Conus amadis, collected off the...
Ananda, K, Aravinda, S, Vasudev, Prema G, Raja, Muruga Poopathi K, Sivaramakrishnan, H, Nagarajan, K, ...
Gabapentin (1-(aminomethyl)cycloheaxaneacetic acid; Gpn) is a widely used anti-epileptic drug. The target site of action of Gpn remains controversial. Gpn can exist in two isomeric chair forms. The...
Registering alpha-Helices and beta-Strands Using Backbone C-H…O Interactions (2003)
Singh, Kumar S, Babu, Madan M, Balaram, P
The possible occurrence of a novel helix terminating structural motif in proteins involving a stabilizing short C-H...O interaction has been examined using a dataset of 634 non-homologous protein...
Parthasarathy, S, Balaram, Hemalatha, Balaram, P, Murthy, MRN
The glycolytic enzymes of Plasmodium falciparum (Pf) are attractive drug targets as the parasites lack a functional tricarboxylic cycle and hence depend heavily on glycolysis for their energy...
Babu, Madan M, Singh, Kumar S, Balaram, P
The serendipitous observation of a C–H· · ·O hydrogen bond mediated polypeptide chain reversal in synthetic peptide helices has led to a search for the occurrence of a similar motif in protein...
Babu, Madan M, Singh, Kumar S, Balaram, P
The serendipitous observation of a C–H···O, hydrogen bond mediated polypeptide chain reversal in synthetic peptide helices has led to a search for the occurrence of a similar motif in protein...
Structural Analysis of Peptide Helices Containing Centrally Positioned Lactic Acid Residues (2002)
Aravinda, S, Shamala, N, Das, Chittaranjan, Balaram, P
The effect of insertion of lactic acid (Lac) residues into peptide helices has been probed using specifically designed sequences. The crystal structures of 11-residue and 14-residue depsipeptides...
Shakkottai, VG, Sudha, R, Balaram, P
Nonenzymatic glycation of proteins has been implicated in various diabetic complications and age-related disorders. Proteins undergo glycation at the N-terminus or at the ε-amino group of lysine...
Maithal, Kapil, Ravindra, Gudihal, Nagaraj, G, Singh, Kumar S, Balaram, Hemalatha, Balaram, P
A mutation at the dimer interface of Plasmodium falciparum triosephosphate isomerase (PfTIM) was created by mutating a tyrosine residue at position 74, at the subunit interface, to glycine. Tyr74 is...
Sudha, R, Panda, M, Chandrasekhar, J, Balaram, P
Apolar, neutral peptides have been shown to ionize extremely well under the conditions used for electrospray ionization mass spectrometry (ESIMS). Peptides for which the conformations have been...
Parthasarathy, S, Balaram, Hemalatha, Balaram, P, Murthy, MRN
The glycolytic enzymes of Plasmodium falciparum (Pf) are attractive drug targets as the parasites lack a functional tricarboxylic cycle and hence depend heavily on glycolysis for their energy...
Structural Analysis of Peptide Helices Containing Centrally Positioned Lactic Acid Residues (2002)
Aravinda, S, Shamala, N, Das, Chittaranjan, Balaram, P
The effect of insertion of lactic acid (Lac) residues into peptide helices has been probed using specifically designed sequences. The crystal structures of 11-residue and 14-residue depsipeptides...
Maithal, Kapil, Ravindra, Gudihal, Nagaraj, G, Singh, Kumar S, Balaram, Hemalatha, Balaram, P
A mutation at the dimer interface of Plasmodium falciparum triosephosphate isomerase (PfTIM) was created by mutating a tyrosine residue at position 74, at the subunit interface, to glycine. Tyr74 is...
Babu, Madan M, Singh, Kumar S, Balaram, P
The serendipitous observation of a C–H···O, hydrogen bond mediated polypeptide chain reversal in synthetic peptide helices has led to a search for the occurrence of a similar motif in protein...
Shakkottai, VG, Sudha, R, Balaram, P
Nonenzymatic glycation of proteins has been implicated in various diabetic complications and age-related disorders. Proteins undergo glycation at the N-terminus or at the ε-amino group of lysine...
Sudha, R, Panda, M, Chandrasekhar, J, Balaram, P
Apolar, neutral peptides have been shown to ionize extremely well under the conditions used for electrospray ionization mass spectrometry (ESIMS). Peptides for which the conformations have been...
Efrapeptins are a group of microheterogeneous polypeptide antibiotics produced by the fungus Tolypocladium niveum, which are potent inhibitors of mitochondrial F1-ATPase. Efrapeptins contain an...
Karle, Isabella L, Das, Chittaranjan, Balaram, P
The insertion of alpha-hydroxy acids into peptide chains provides a convenient means for investigating the effects of hydrogen bond deletion on polypeptide secondary structures. The crystal...
Singh, Kumar S, Maithal, Kapil, Balaram, Hemalatha, Balaram, P
Synthetic peptides corresponding to two distinct segments of the subunit interface of the homodimeric enzyme triosephosphate isomerase (residues 9–18, ANWKCNGTLE, peptide I; residues 68–79,...
Venkatraman, Janani, Aggarwal, Kamna, Balaram, P
Introduction: Non-enzymatic glycation of proteins has been implicated in various diabetic complications and age-related disorders. Proteins undergo glycation at the N-terminus or at the O-amino group...
Ray, Soumya S, Singh, Kumar S, Balaram, P
The binding of 1-anilino-8-naphthalene-sulfonic acid (ANS) to various globular proteins at acidic pH has been investigated by electrospray ionization mass spectrometry (ESI-MS). Maximal ANS binding...
Designed beta-Hairpin Peptides with Defined Tight Turn Stereochemistry (2001)
Das, Chittaranjan, Naganagowda, GA, Karle, Isabella L, Balaram, P
The conformational analysis of two synthetic octapeptides, Boc–Leu–Val–Val–D-Pro–L-Ala–Leu–Val–Val–OMe (1) and Boc–Leu–Val–Val–D-Pro–D-Ala–Leu–Val–Val–OMe (2) has...
Solvent-induced Beta-hairpin to helix conformational transition in a designed peptide (2001)
Awasthi, Satish K, Shankaramma, SC, Raghothama, S, Balaram, P
An octapeptide containing a central Aib-Gly- segment capable of adopting -turn conformations compatible with both hairpin ( II or I ) and helical ( I) structures has been designed. The effect of...
Efrapeptins are a group of microheterogeneous polypeptide antibiotics produced by the fungus Tolypocladium niveum, which are potent inhibitors of mitochondrial F1-ATPase. Efrapeptins contain an...
Singh, Kumar S, Maithal, Kapil, Balaram, Hemalatha, Balaram, P
Synthetic peptides corresponding to two distinct segments of the subunit interface of the homodimeric enzyme triosephosphate isomerase (residues 9–18, ANWKCNGTLE, peptide I; residues 68–79,...
Karle, Isabella L, Das, Chittaranjan, Balaram, P
The insertion of alpha-hydroxy acids into peptide chains provides a convenient means for investigating the effects of hydrogen bond deletion on polypeptide secondary structures. The crystal...
Ray, Soumya S, Singh, Kumar S, Balaram, P
The binding of 1-anilino-8-naphthalene-sulfonic acid (ANS) to various globular proteins at acidic pH has been investigated by electrospray ionization mass spectrometry (ESI-MS). Maximal ANS binding...
Venkatraman, Janani, Aggarwal, Kamna, Balaram, P
Introduction: Non-enzymatic glycation of proteins has been implicated in various diabetic complications and age-related disorders. Proteins undergo glycation at the N-terminus or at the O-amino group...
Designed beta-Hairpin Peptides with Defined Tight Turn Stereochemistry (2001)
Das, Chittaranjan, Naganagowda, GA, Karle, Isabella L, Balaram, P
The conformational analysis of two synthetic octapeptides, Boc–Leu–Val–Val–D-Pro–L-Ala–Leu–Val–Val–OMe (1) and Boc–Leu–Val–Val–D-Pro–D-Ala–Leu–Val–Val–OMe (2) has...
Genotoxicity of human breast milk from different countries. (2001)
Phillips, David H., Martin, Francis L., Grover, Philip L., Cole, Kathleen J., Weaver, Gillian, Hong, Ga S., ...
Solvent-induced Beta-hairpin to helix conformational transition in a designed peptide (2001)
Awasthi, Satish K, Shankaramma, SC, Raghothama, S, Balaram, P
An octapeptide containing a central Aib-Gly- segment capable of adopting -turn conformations compatible with both hairpin ( II or I ) and helical ( I) structures has been designed. The effect of...
Das, C, Nayak, V, Balaram, P, Raghothama, S
The characterization of a four-stranded $\beta$-sheet structure in a designed 26-residue peptide Beta-4 is described. The sequence of Beta-4...
Ratnaparkhi, Girish S, Awasthi, Satish Kumar, Rani, P, Balaram, P, Varadarajan, R
The S protein–S peptide interaction is a model system to study binding thermodynamics in proteins. We substituted alanine at position 4 in S peptide by alpha-aminoisobutyric acid (Aib) to...
Kaul, Ramesh, Banumathi, S, Velmurugan, D, Rao, Balaji R, Balaram, P
The conformational analysis of peptides containing a single alpha,alpha-di-n-propylglycine (Dpg) residue incorporated into valine-rich sequences has been undertaken in order to delineate the possible...
Vibrational Circular Dichroism of beta-Hairpin Peptides (2000)
Zhao, Chunxia, Polavarapu, Prasad L, Das, Chittaranjan, Balaram, P
Analysis of vibrational absorption and vibrational circular dichroism (VCD) for synthetic peptides designed to adopt beta-hairpin conformations reveals characteristic well-resolved amide I absorption...
Aravinda, S, Shamala, N, Pramanik, Animesh, Das, Chittaranjan, Balaram, P
An unusual C-terminal conformation has been detected in a synthetic decapeptide designed to analyze the stereochemistry of helix termination in polypeptides. The crystal structure of the decapeptide...
Context-dependent conformation of diethylglycine residues in peptides (2000)
Kaul, R, Balaram, P, Banumathi, S, Velmurugan, D, Ravikumar, K, Rao, Balaji R
Diethylglycine (Deg) residues incorporated into peptides can stabilize fully extended $(C_5)$ or helical conformations. The conformations of three tetrapeptides Boc-Xxx-Deg-Xxx-Deg-OMe (Xxx = Gly,...
Aravinda, S, Shamala, N, Pramanik, Animesh, Das, Chittaranjan, Balaram, P
An unusual C-terminal conformation has been detected in a synthetic decapeptide designed to analyze the stereochemistry of helix termination in polypeptides. The crystal structure of the decapeptide...