P. C. Sternweis

Phospholipase D is present on Golgi-enriched membranes and its activation by ADP ribosylation factor is sensitive to brefeldin A.

Ktistakis, N T, Brown, H A, Sternweis, P C, Roth, M G

ADP ribosylation factor (ARF) is a small guanosine triphosphate (GTP)-binding protein that regulates the binding of coat proteins to membranes and is required for several stages of vesicular...

Purification and characterization of recombinant G16 alpha from Sf9 cells: activation of purified phospholipase C isozymes by G-protein alpha subunits.

Kozasa, T, Hepler, J R, Smrcka, A V, Simon, M I, Rhee, S G, Sternweis, P C, ...

A cDNA encoding G16 alpha, the alpha subunit of a heterotrimeric guanine nucleotide-binding protein, was expressed in Sf9 cells using recombinant baculovirus. G16 alpha in membrane extracts of Sf9...

G protein gamma subunits contain a 20-carbon isoprenoid.

Mumby, S M, Casey, P J, Gilman, A G, Gutowski, S, Sternweis, P C

A small subset of cellular proteins are covalently modified by the addition of isoprenoid groups. These include p21ras, fungal mating factors, and nuclear lamins, which are isoprenylated at...

Influence of gamma subunit prenylation on association of guanine nucleotide-binding regulatory proteins with membranes.

Muntz, K H, Sternweis, P C, Gilman, A G, Mumby, S M

Two approaches were taken to address the possible role of gamma-subunit prenylation in dictating the cellular distribution of guanine nucleotide-binding regulatory proteins. Prenylation of gamma...

Guanine nucleotide-binding protein Go-induced coupling of neuropeptide Y receptors to Ca2+ channels in sensory neurons.

Ewald, D A, Sternweis, P C, Miller, R J

Neuropeptide Y (NPY) inhibited the Ca2+ current (ICa) in rat dorsal root ganglion neurons in vitro. NPY inhibited the sustained ICa evoked by steps to 0 mV from a holding potential of -40 mV and the...

Isolation of the alpha subunits of GTP-binding regulatory proteins by affinity chromatography with immobilized beta gamma subunits.

Pang, I H, Sternweis, P C

Immobilized beta gamma subunits of GTP-binding regulatory proteins (G proteins) were used to isolate alpha subunits from solubilized membranes of bovine tissues and to separate specific alpha...

Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.

Sternweis, P C, Gilman, A G

Activation of the purified guanine nucleotide-binding regulatory component (G/F) of adenylate cyclase by F- requires the presence of Mg2+ and another factor. This factor, which contaminates...

Purification of the regulatory component of adenylate cyclase.

Northup, J K, Sternweis, P C, Smigel, M D, Schleifer, L S, Ross, E M, Gilman, A G

The regulatory component (G/F) of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] from rabbit liver plasma membranes has been purified essentially to homogeneity. The purification...

Phospholipase D is present on Golgi-enriched membranes and its activation by ADP ribosylation factor is sensitive to brefeldin A.

Ktistakis, N T, Brown, H A, Sternweis, P C, Roth, M G

ADP ribosylation factor (ARF) is a small guanosine triphosphate (GTP)-binding protein that regulates the binding of coat proteins to membranes and is required for several stages of vesicular...

Purification and characterization of recombinant G16 alpha from Sf9 cells: activation of purified phospholipase C isozymes by G-protein alpha subunits.

Kozasa, T, Hepler, J R, Smrcka, A V, Simon, M I, Rhee, S G, Sternweis, P C, ...

A cDNA encoding G16 alpha, the alpha subunit of a heterotrimeric guanine nucleotide-binding protein, was expressed in Sf9 cells using recombinant baculovirus. G16 alpha in membrane extracts of Sf9...

G protein gamma subunits contain a 20-carbon isoprenoid.

Mumby, S M, Casey, P J, Gilman, A G, Gutowski, S, Sternweis, P C

A small subset of cellular proteins are covalently modified by the addition of isoprenoid groups. These include p21ras, fungal mating factors, and nuclear lamins, which are isoprenylated at...

Influence of gamma subunit prenylation on association of guanine nucleotide-binding regulatory proteins with membranes.

Muntz, K H, Sternweis, P C, Gilman, A G, Mumby, S M

Two approaches were taken to address the possible role of gamma-subunit prenylation in dictating the cellular distribution of guanine nucleotide-binding regulatory proteins. Prenylation of gamma...

Guanine nucleotide-binding protein Go-induced coupling of neuropeptide Y receptors to Ca2+ channels in sensory neurons.

Ewald, D A, Sternweis, P C, Miller, R J

Neuropeptide Y (NPY) inhibited the Ca2+ current (ICa) in rat dorsal root ganglion neurons in vitro. NPY inhibited the sustained ICa evoked by steps to 0 mV from a holding potential of -40 mV and the...

Isolation of the alpha subunits of GTP-binding regulatory proteins by affinity chromatography with immobilized beta gamma subunits.

Pang, I H, Sternweis, P C

Immobilized beta gamma subunits of GTP-binding regulatory proteins (G proteins) were used to isolate alpha subunits from solubilized membranes of bovine tissues and to separate specific alpha...

Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.

Sternweis, P C, Gilman, A G

Activation of the purified guanine nucleotide-binding regulatory component (G/F) of adenylate cyclase by F- requires the presence of Mg2+ and another factor. This factor, which contaminates...

Purification of the regulatory component of adenylate cyclase.

Northup, J K, Sternweis, P C, Smigel, M D, Schleifer, L S, Ross, E M, Gilman, A G

The regulatory component (G/F) of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] from rabbit liver plasma membranes has been purified essentially to homogeneity. The purification...