Asymmetry in Flp-mediated cleavage.
Luetke, K H, Zhao, B P, Sadowski, P D
Flp is a member of the integrase family of site-specific recombinases. Members of the integrase family mediate DNA strand cleavage via a transesterification reaction involving an active site tyrosine...
Flp is a member of the integrase family of site-specific recombinases. Flp is known to be a double-stranded (ds)DNA binding protein that binds sequence specifically to the 13 bp binding elements in...
Determinants of the position of a Flp-induced DNA bend.
The Flp site-specific recombinase from Saccharomyces cerevisiae induces DNA bending upon interaction with the Flp recognition target (FRT) site. The minimal FRT site is comprised of two inverted...
In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay.
We developed a simple, direct, physical assay to detect genetic recombination of bacteriophage T7 DNA in vitro. In this assay two mature T7 DNA molecules, each having a unique restriction enzyme...
Site-specific cleavages of intracellular DNA were demonstrated in bacteriophage T7 6am-infected cells. The sites of the cleavages were located at 46.8 and 68.7% (1% of the T7 DNA length = 400 base...
Genetic recombination of bacteriophage T7 in vivo studied by use of a simple physical assay.
A new physical method was developed to assay genetic recombination of phage T7 in vivo. The assay utilized T7 mutants that carry unique restriction sites and was based on the detection of a new...
The FLP protein contacts both major and minor grooves of its recognition target sequence.
Panigrahi, G B, Beatty, L G, Sadowski, P D
The FLP protein of the 2 microns plasmid of Saccharomyces cerevisiae promotes conservative site-specific recombination between DNA sequences that contain the FLP recognition target (FRT). FLP binds...
Genetic recombination of bacteriophage T7 DNA in vitro.
We have demonstrated recombination of bacteriophage T7 DNA in vitro. An extract of Escherichia coli B cells infected with wild-type T7(T7+) is incubated with mature DNA extracted from T7 phage....
Point Mutants in the D2a Region of Bacteriophage T4 Fail to Induce T4 Endonuclease IV
We have studied the properties of presumptive point mutants in the D2a region of bacteriophage T4. Dominance tests showed that the D2a mutation was recessive to the wild-type allele. The mutations...
Pan, G, Luetke, K, Sadowski, P D
The FLP recombinase of the 2 microns plasmid of Saccharomyces cerevisiae is a member of the integrase family of site-specific recombinases. Recombination catalyzed by members of this family proceeds...
Amin, A, Roca, H, Luetke, K, Sadowski, P D
We have used a previously described cross-linking assay and half-FRT site substrates to examine the requirements for synapsis, strand exchange, and strand scission. The cross-linking assay showed...
Synthesis of an enzymatically active FLP recombinase in vitro: search for a DNA-binding domain.
We have used an in vitro transcription and translation system to synthesize an enzymatically active FLP protein. The FLP mRNA synthesized in vitro by SP6 polymerase is translated efficiently in a...
Andrews, B J, McLeod, M, Broach, J, Sadowski, P D
The 2 micron plasmid of Saccharomyces cerevisiae codes for a site-specific recombinase, the FLP protein, that catalyzes efficient recombination across two 599-base-pair (bp) inverted repeats of the...
Gronostajski, R M, Sadowski, P D
The FLP recombinase, encoded by the 2 micron plasmid of Saccharomyces cerevisiae, promotes efficient recombination in vivo and in vitro between its specific target sites (FLP sites). It was...
Site-specific recombination of yeast 2-micron DNA in vitro.
Vetter, D, Andrews, B J, Roberts-Beatty, L, Sadowski, P D
Most strains of the yeast Saccharomyces cerevisiae harbor several copies of a 2-micron plasmid circle DNA termed "2 micron." This circular plasmid contains two 599-base-pair precise inverted repeats...
Vetter, D, Roberts, L, Jackowski, J B, Sadowski, P D
T7+ phage are unable to plate on a strain of Shigella sonnei D2 371-48. Spontaneous phage mutants arise (ss--mutants) that are able to plate on this strain of Shigella. We have shown by...
Asymmetry in Flp-mediated cleavage.
Luetke, K H, Zhao, B P, Sadowski, P D
Flp is a member of the integrase family of site-specific recombinases. Members of the integrase family mediate DNA strand cleavage via a transesterification reaction involving an active site tyrosine...
Flp is a member of the integrase family of site-specific recombinases. Flp is known to be a double-stranded (ds)DNA binding protein that binds sequence specifically to the 13 bp binding elements in...
Determinants of the position of a Flp-induced DNA bend.
The Flp site-specific recombinase from Saccharomyces cerevisiae induces DNA bending upon interaction with the Flp recognition target (FRT) site. The minimal FRT site is comprised of two inverted...
In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay.
We developed a simple, direct, physical assay to detect genetic recombination of bacteriophage T7 DNA in vitro. In this assay two mature T7 DNA molecules, each having a unique restriction enzyme...
Site-specific cleavages of intracellular DNA were demonstrated in bacteriophage T7 6am-infected cells. The sites of the cleavages were located at 46.8 and 68.7% (1% of the T7 DNA length = 400 base...
Genetic recombination of bacteriophage T7 in vivo studied by use of a simple physical assay.
A new physical method was developed to assay genetic recombination of phage T7 in vivo. The assay utilized T7 mutants that carry unique restriction sites and was based on the detection of a new...
The FLP protein contacts both major and minor grooves of its recognition target sequence.
Panigrahi, G B, Beatty, L G, Sadowski, P D
The FLP protein of the 2 microns plasmid of Saccharomyces cerevisiae promotes conservative site-specific recombination between DNA sequences that contain the FLP recognition target (FRT). FLP binds...
Genetic recombination of bacteriophage T7 DNA in vitro.
We have demonstrated recombination of bacteriophage T7 DNA in vitro. An extract of Escherichia coli B cells infected with wild-type T7(T7+) is incubated with mature DNA extracted from T7 phage....
Point Mutants in the D2a Region of Bacteriophage T4 Fail to Induce T4 Endonuclease IV
We have studied the properties of presumptive point mutants in the D2a region of bacteriophage T4. Dominance tests showed that the D2a mutation was recessive to the wild-type allele. The mutations...
Pan, G, Luetke, K, Sadowski, P D
The FLP recombinase of the 2 microns plasmid of Saccharomyces cerevisiae is a member of the integrase family of site-specific recombinases. Recombination catalyzed by members of this family proceeds...
Amin, A, Roca, H, Luetke, K, Sadowski, P D
We have used a previously described cross-linking assay and half-FRT site substrates to examine the requirements for synapsis, strand exchange, and strand scission. The cross-linking assay showed...
Synthesis of an enzymatically active FLP recombinase in vitro: search for a DNA-binding domain.
We have used an in vitro transcription and translation system to synthesize an enzymatically active FLP protein. The FLP mRNA synthesized in vitro by SP6 polymerase is translated efficiently in a...
Andrews, B J, McLeod, M, Broach, J, Sadowski, P D
The 2 micron plasmid of Saccharomyces cerevisiae codes for a site-specific recombinase, the FLP protein, that catalyzes efficient recombination across two 599-base-pair (bp) inverted repeats of the...
Gronostajski, R M, Sadowski, P D
The FLP recombinase, encoded by the 2 micron plasmid of Saccharomyces cerevisiae, promotes efficient recombination in vivo and in vitro between its specific target sites (FLP sites). It was...
Site-specific recombination of yeast 2-micron DNA in vitro.
Vetter, D, Andrews, B J, Roberts-Beatty, L, Sadowski, P D
Most strains of the yeast Saccharomyces cerevisiae harbor several copies of a 2-micron plasmid circle DNA termed "2 micron." This circular plasmid contains two 599-base-pair precise inverted repeats...
Vetter, D, Roberts, L, Jackowski, J B, Sadowski, P D
T7+ phage are unable to plate on a strain of Shigella sonnei D2 371-48. Spontaneous phage mutants arise (ss--mutants) that are able to plate on this strain of Shigella. We have shown by...