Wilkinson, D., Akumanyi, N., Hurtado-Guerrero, R., Dawkes, H., Knowles, P.F., Phillips, S.E.V., ...
Galactose oxidase (GO; E.C. 1.1.3.9) is a copper‐ containing enzyme that oxidizes a range of primary alcohols to aldehydes. This broad substrate specificity is reflected in a high KM for...
Wilkinson, D, Akumanyi, N., Hurtado-Guerrero, R., Dawkes, H., Knowles, P.F., Phillips, S.E.V., ...
Galactose oxidase (GO; E.C. 1.1.3.9) is a copper-containing enzyme that oxidises a range of primary alcohols to aldehydes. This broad substrate specificity is reflected in a high KM for substrates....
Wilkinson, D, Akumanyi, N., Hurtado-Guerrero, R., Dawkes, H., Knowles, P.F., Phillips, S.E.V., ...
Galactose oxidase (GO; E.C. 1.1.3.9) is a copper-containing enzyme that oxidises a range of primary alcohols to aldehydes. This broad substrate specificity is reflected in a high KM for substrates....
HALSALL, P. J., ELLIS, F. R., KNOWLES, P. F.
We have evaluated a spin labelled electron spin resonance technique to identify malignant hyper-thermia susceptible (MHS) patients. We studied 19 patients, 10 MHS and nine MHN (normal), using the...
AN EXAFS STUDY OF THE COPPER AND ZINC SITES OF LYOPHILIZED BOVINE SUPEROXIDE DISMUTASE (1983)
Blackburn, N J, Hasnain, S S, Diakun, G P, Knowles, P F, Binstead, N, Garner, C D
Crystal structure of the precursor of galactose oxidase: An unusual self-processing enzyme
Firbank, S. J., Rogers, M. S., Wilmot, C. M., Dooley, D. M., Halcrow, M. A., Knowles, P. F., ...
Galactose oxidase (EC 1.1.3.9) is a monomeric enzyme that contains a single copper ion and catalyses the stereospecific oxidation of primary alcohols to their corresponding aldehydes. The protein...
Cooper, R A, Knowles, P F, Brown, D E, McGuirl, M A, Dooley, D M
The cofactors present in a amine oxidase induced in Escherichia coli K-12 by growth on 2-phenylethylamine have been studied by spectroscopic methods. E.s.r. spectroscopy establishes the presence of...
Studies on the active site of pig plasma amine oxidase.
Collison, D, Knowles, P F, Mabbs, F E, Rius, F X, Singh, I, Dooley, D M, ...
Amine oxidase from pig plasma (PPAO) has two bound Cu2+ ions and at least one pyrroloquinoline quinone (PQQ) moiety as cofactors. It is shown that recovery of activity by copper-depleted PPAO is...
Hasnain, S S, Diakun, G P, Knowles, P F, Binsted, N, Garner, C D, Blackburn, N J
Copper K-edge e.x.a.f.s (extended X-ray-absorption fine structure) was measured for dopamine beta-mono-oxygenase in aqueous solution. Comparison with the Cu K-edge e.x.a.f.s. of bovine erythrocyte...
Electron nuclear double-resonance (ENDOR) spectroscopy of amine oxidase from pig plasma.
Baker, G J, Knowles, P F, Pandeya, K B, Rayner, J B
Electron nuclear double-resonance ('ENDOR') spectroscopic studies on pig plasma amine oxidase have been carried out at 15 K. Deuterium-exchange studies show the presence of two sets of exchangeable...
Knowles, P F, Pandeya, K B, Rius, F X, Spencer, C M, Moog, R S, McGuirl, M A, ...
Plasma amine oxidases (EC 1.4.3.6) are classified as containing the organic cofactor pyridoxal phosphate. Biochemical and bioassays on the pig plasma amine oxidase fail to reveal the presence of...
Blackburn, N J, Hasnain, S S, Diakun, G P, Knowles, P F, Binsted, N, Garner, C D
Copper and zinc K-edge-extended X-ray-absorption fine structures were measured for the metal sites of freeze-dried bovine superoxide dismutase and the model compounds tetrakis(imidazole)cupric...
Blackburn, N J, Hasnain, S S, Binsted, N, Diakun, G P, Garner, C D, Knowles, P F
Copper and zinc K-edge e.x.a.f.s. (extended X-ray-absorption fine structures) were measured for the metal sites of oxidized and reduced bovine superoxide dismutase in aqueous solution. Detailed...
The kinetics of ammonia release during the catalytic cycle of pig plasma amine oxidase.
Rius, F X, Knowles, P F, Pettersson, G
The time course of NH3 release during the catalytic cycle of pig plasma amine oxidase was followed by using the quenched-flow technique in conjunction with a sensitive assay for NH3. These studies...
Crabbe, M J, Waight, R D, Bardsley, W G, Barker, R W, Kelly, I D, Knowles, P F
1. Isoelectric focusing studies of human placental diamine oxidase showed the pI value of the active enzyme to be 6.5. This information was used in modifying the enzyme purification by incorporating...
A catalytic mechanism for the enzyme benzylamine oxidase from pig plasma
Taylor, C. E., Taylor, R. S., Rasmussen, C., Knowles, P. F.
Initial-velocity and product-inhibition studies on the enzyme benzylamine oxidase from pig plasma indicate that the order of substrate addition and product release is benzylamine on, ammonia off,...
Horváth, L I, Knowles, P F, Kovachev, P, Findlay, J B, Marsh, D
Lipid-peptide interactions with the 27-residue peptide of sequence KLEALYILMVLGFFGFFTLGIMLSYIR reconstituted as beta-sheet assemblies in dimyristoylphosphatidylcholine bilayers have been studied by...
Cytochrome oxidase, purified from the yeast Saccharomyces cerevisiae, was shown to have associated phospholipid, cholate or detergent, which could be varied by dialysis or (NH4)2SO4 precipitation of...
Barker, R, Boden, N, Cayley, G, Charlton, S C, Henson, R, Holmes, M C, ...
Benzylamine oxidase from pig plasma has been studied by a variety of chemical and physical techniques. 1. Analytical ultracentrifugation, gel electrophoresis and isoelectric-focusing studies suggest...
Boden, N, Holmes, M C, Knowles, P F
Proton nuclear-relaxation rates have been measured as a function of frequency, temperature, pH and cyanide concentration in aqueous solutions of superoxide dismutase from bovine erythrocytes. The...
Knowles, P. F., Gibson, J. F., Pick, F. M., Bray, R. C.
1. An electron-spin-resonance signal with g∥2·08 and g⊥2·00 is observed by the rapid-freezing technique during the oxidation of substrates by molecular oxygen catalysed by xanthine oxidase at...
ESR spin-label studies of lipid-protein interactions in membranes.
Marsh, D, Watts, A, Pates, R D, Uhl, R, Knowles, P F, Esmann, M
Lipid spin labels have been used to study lipid-protein interactions in bovine and frog rod outer segment disc membranes, in (Na+, K+)-ATPase membranes from shark rectal gland, and in yeast...
Crystal structure of the precursor of galactose oxidase: An unusual self-processing enzyme
Firbank, S. J., Rogers, M. S., Wilmot, C. M., Dooley, D. M., Halcrow, M. A., Knowles, P. F., ...
Galactose oxidase (EC 1.1.3.9) is a monomeric enzyme that contains a single copper ion and catalyses the stereospecific oxidation of primary alcohols to their corresponding aldehydes. The protein...
Cooper, R A, Knowles, P F, Brown, D E, McGuirl, M A, Dooley, D M
The cofactors present in a amine oxidase induced in Escherichia coli K-12 by growth on 2-phenylethylamine have been studied by spectroscopic methods. E.s.r. spectroscopy establishes the presence of...
Studies on the active site of pig plasma amine oxidase.
Collison, D, Knowles, P F, Mabbs, F E, Rius, F X, Singh, I, Dooley, D M, ...
Amine oxidase from pig plasma (PPAO) has two bound Cu2+ ions and at least one pyrroloquinoline quinone (PQQ) moiety as cofactors. It is shown that recovery of activity by copper-depleted PPAO is...
Hasnain, S S, Diakun, G P, Knowles, P F, Binsted, N, Garner, C D, Blackburn, N J
Copper K-edge e.x.a.f.s (extended X-ray-absorption fine structure) was measured for dopamine beta-mono-oxygenase in aqueous solution. Comparison with the Cu K-edge e.x.a.f.s. of bovine erythrocyte...
Electron nuclear double-resonance (ENDOR) spectroscopy of amine oxidase from pig plasma.
Baker, G J, Knowles, P F, Pandeya, K B, Rayner, J B
Electron nuclear double-resonance ('ENDOR') spectroscopic studies on pig plasma amine oxidase have been carried out at 15 K. Deuterium-exchange studies show the presence of two sets of exchangeable...
Knowles, P F, Pandeya, K B, Rius, F X, Spencer, C M, Moog, R S, McGuirl, M A, ...
Plasma amine oxidases (EC 1.4.3.6) are classified as containing the organic cofactor pyridoxal phosphate. Biochemical and bioassays on the pig plasma amine oxidase fail to reveal the presence of...
Blackburn, N J, Hasnain, S S, Diakun, G P, Knowles, P F, Binsted, N, Garner, C D
Copper and zinc K-edge-extended X-ray-absorption fine structures were measured for the metal sites of freeze-dried bovine superoxide dismutase and the model compounds tetrakis(imidazole)cupric...
Blackburn, N J, Hasnain, S S, Binsted, N, Diakun, G P, Garner, C D, Knowles, P F
Copper and zinc K-edge e.x.a.f.s. (extended X-ray-absorption fine structures) were measured for the metal sites of oxidized and reduced bovine superoxide dismutase in aqueous solution. Detailed...
The kinetics of ammonia release during the catalytic cycle of pig plasma amine oxidase.
Rius, F X, Knowles, P F, Pettersson, G
The time course of NH3 release during the catalytic cycle of pig plasma amine oxidase was followed by using the quenched-flow technique in conjunction with a sensitive assay for NH3. These studies...
Crabbe, M J, Waight, R D, Bardsley, W G, Barker, R W, Kelly, I D, Knowles, P F
1. Isoelectric focusing studies of human placental diamine oxidase showed the pI value of the active enzyme to be 6.5. This information was used in modifying the enzyme purification by incorporating...
A catalytic mechanism for the enzyme benzylamine oxidase from pig plasma
Taylor, C. E., Taylor, R. S., Rasmussen, C., Knowles, P. F.
Initial-velocity and product-inhibition studies on the enzyme benzylamine oxidase from pig plasma indicate that the order of substrate addition and product release is benzylamine on, ammonia off,...
Horváth, L I, Knowles, P F, Kovachev, P, Findlay, J B, Marsh, D
Lipid-peptide interactions with the 27-residue peptide of sequence KLEALYILMVLGFFGFFTLGIMLSYIR reconstituted as beta-sheet assemblies in dimyristoylphosphatidylcholine bilayers have been studied by...
Cytochrome oxidase, purified from the yeast Saccharomyces cerevisiae, was shown to have associated phospholipid, cholate or detergent, which could be varied by dialysis or (NH4)2SO4 precipitation of...
Barker, R, Boden, N, Cayley, G, Charlton, S C, Henson, R, Holmes, M C, ...
Benzylamine oxidase from pig plasma has been studied by a variety of chemical and physical techniques. 1. Analytical ultracentrifugation, gel electrophoresis and isoelectric-focusing studies suggest...
Boden, N, Holmes, M C, Knowles, P F
Proton nuclear-relaxation rates have been measured as a function of frequency, temperature, pH and cyanide concentration in aqueous solutions of superoxide dismutase from bovine erythrocytes. The...
Knowles, P. F., Gibson, J. F., Pick, F. M., Bray, R. C.
1. An electron-spin-resonance signal with g∥2·08 and g⊥2·00 is observed by the rapid-freezing technique during the oxidation of substrates by molecular oxygen catalysed by xanthine oxidase at...
ESR spin-label studies of lipid-protein interactions in membranes.
Marsh, D, Watts, A, Pates, R D, Uhl, R, Knowles, P F, Esmann, M
Lipid spin labels have been used to study lipid-protein interactions in bovine and frog rod outer segment disc membranes, in (Na+, K+)-ATPase membranes from shark rectal gland, and in yeast...