P. F. Searle

Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA.

Smith, D F, Searle, P F, Williams, J G

A 1700 nucleotide DNA sequence derived from Xenopus vitellogenin mRNA has been cloned in the bacterial plasmid pBR322. The identity of the cloned sequence was verified in two ways. Firstly, the...

Zinc dependent binding of a liver nuclear factor to metal response element MRE-a of the mouse metallothionein-I gene and variant sequences.

Searle, P F

Metallothionein gene transcription is inducible by zinc and other heavy metals, and several metal response elements (MREs) have been mapped within about 200 bp upstream of the site of transcription...

Expression of a human O6-alkylguanine-DNA-alkyltransferase cDNA in human cells and transgenic mice.

Fan, C Y, Potter, P M, Rafferty, J, Watson, A J, Cawkwell, L, Searle, P F, ...

A truncated human O6-alkylguanine-DNA-alkyltransferase (ATase) cDNA was ligated into an expression vector under the control of the mouse metallothionein-1 gene promotor and upstream of part of the...

Building a metal-responsive promoter with synthetic regulatory elements.

Searle, P F, Stuart, G W, Palmiter, R D

A fusion gene consisting of the promoter region from the mouse metallothionein-I gene joined to the coding region of the herpes simplex virus thymidine kinase gene is efficiently regulated by zinc in...

Regulation, linkage, and sequence of mouse metallothionein I and II genes.

Searle, P F, Davison, B L, Stuart, G W, Wilkie, T M, Norstedt, G, Palmiter, R D

The mouse metallothionein II (MT-II) gene is located approximately 6 kilobases upstream of the MT-I gene. A comparison of the sequences of mouse MT-I and MT-II genes (as well as those of other...

A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene.

Stuart, G W, Searle, P F, Chen, H Y, Brinster, R L, Palmiter, R D

To define DNA sequences involved in mouse metallothionein-I (MT-I) gene promoter function and metal regulation, we fused the 5' flanking sequences of the MT-I gene to the coding sequences of a viral...

Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA.

Smith, D F, Searle, P F, Williams, J G

A 1700 nucleotide DNA sequence derived from Xenopus vitellogenin mRNA has been cloned in the bacterial plasmid pBR322. The identity of the cloned sequence was verified in two ways. Firstly, the...

Zinc dependent binding of a liver nuclear factor to metal response element MRE-a of the mouse metallothionein-I gene and variant sequences.

Searle, P F

Metallothionein gene transcription is inducible by zinc and other heavy metals, and several metal response elements (MREs) have been mapped within about 200 bp upstream of the site of transcription...

Expression of a human O6-alkylguanine-DNA-alkyltransferase cDNA in human cells and transgenic mice.

Fan, C Y, Potter, P M, Rafferty, J, Watson, A J, Cawkwell, L, Searle, P F, ...

A truncated human O6-alkylguanine-DNA-alkyltransferase (ATase) cDNA was ligated into an expression vector under the control of the mouse metallothionein-1 gene promotor and upstream of part of the...

Building a metal-responsive promoter with synthetic regulatory elements.

Searle, P F, Stuart, G W, Palmiter, R D

A fusion gene consisting of the promoter region from the mouse metallothionein-I gene joined to the coding region of the herpes simplex virus thymidine kinase gene is efficiently regulated by zinc in...

Regulation, linkage, and sequence of mouse metallothionein I and II genes.

Searle, P F, Davison, B L, Stuart, G W, Wilkie, T M, Norstedt, G, Palmiter, R D

The mouse metallothionein II (MT-II) gene is located approximately 6 kilobases upstream of the MT-I gene. A comparison of the sequences of mouse MT-I and MT-II genes (as well as those of other...

A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene.

Stuart, G W, Searle, P F, Chen, H Y, Brinster, R L, Palmiter, R D

To define DNA sequences involved in mouse metallothionein-I (MT-I) gene promoter function and metal regulation, we fused the 5' flanking sequences of the MT-I gene to the coding sequences of a viral...