72 Multiservice Ethernet access (2008)
P Gunning, M Wilkinson, L Rragami, S Semnani, K Smith
Ethernet is evolving from its origins in the local area network into a cost-effective and reliable wide area network technology. Market research shows that business customers are moving away from...
Swindells, C., Gunning, P., Wang, W., Grün, C., Mahabir, K., ...
High-performance liquid chromatography (HPLC)-UV and HPLC–Mass Spectrometry (MS) methods were developed for the quantitative analysis of the family of Hoodia gordonii steroid glycosides with...
Specific isoforms of actin-binding proteins on distinct populations of Golgi-derived vesicles (1999)
Specific isoforms of actin-binding proteins on distinct populations of Golgi-derived vesicles (1999)
Heimann, K. R., Percival, J. M., Weinberger, R., Gunning, P., Stow, J. L.
Golgi membranes and Golgi-derived vesicles are associated with multiple cytoskeletal proteins and motors, the diversity and distribution of which have not yet been defined. Carrier vesicles were...
Specific isoforms of actin-binding proteins on distinct populations of Golgi-derived vesicles (1999)
Heimann, K. R., Percival, J. M., Weinberger, R., Gunning, P., Stow, J. L.
Golgi membranes and Golgi-derived vesicles are associated with multiple cytoskeletal proteins and motors, the diversity and distribution of which have not yet been defined. Carrier vesicles were...
Gunning, P, Leavitt, J, Muscat, G, Ng, S Y, Kedes, L
We have constructed a mammalian expression vector consisting of 3 kilobases of the human beta-actin gene 5' flanking sequence plus 5' untranslated region and intervening sequence 1 linked at the 3'...
Erba, H P, Gunning, P, Kedes, L
Two distinct, but iso-coding, gamma non-muscle actin cDNAs were isolated from an SV40-transformed human fibroblast library. The complete nucleotide sequence of the human gamma non-muscle actin cDNAs...
Ponte, P, Ng, S Y, Engel, J, Gunning, P, Kedes, L
We report the complete nucleotide sequence of a human beta actin cDNA. Both the 5' and 3' untranslated regions of the sequence are similar (greater than 80%) to the analogous regions of the rat...
Regulation of the human beta-actin promoter by upstream and intron domains.
Ng, S Y, Gunning, P, Liu, S H, Leavitt, J, Kedes, L
We have identified three regulatory domains of the complex human beta-actin gene promoter. They span a region of about 3000 bases, from not more than -2011 bases upstream of the mRNA cap site to...
Chromosomal location of the co-expressed human skeletal and cardiac actin genes.
Gunning, P, Ponte, P, Kedes, L, Eddy, R, Shows, T
We have examined the relationship between chromosomal location and regulation of the two human genes encoding the sarcomeric muscle actins. The human genes encoding skeletal alpha-actin and cardiac...
Gamma Tropomyosin Gene Products Are Required for Embryonic Development
Hook, J., Lemckert, F., Qin, H., Schevzov, G., Gunning, P.
The actin filament system is essential for many cellular functions, including shape, motility, cytokinesis, intracellular trafficking, and tissue organization. Tropomyosins (Tms) are rod-like...
Erba, H P, Eddy, R, Shows, T, Kedes, L, Gunning, P
The accumulation of the cytoskeletal beta- and gamma-actin mRNAs was determined in a variety of mouse tissues and organs. The beta-isoform is always expressed in excess of the gamma-isoform. However,...
Leavitt, J, Ng, S Y, Aebi, U, Varma, M, Latter, G, Burbeck, S, ...
Two different mutant human beta-actin genes have been introduced into normal diploid human (KD) fibroblasts and their immortalized derivative cell line, HuT-12, to assess the impact of an abnormal...
Expression of transfected mutant beta-actin genes: transitions toward the stable tumorigenic state.
Leavitt, J, Ng, S Y, Varma, M, Latter, G, Burbeck, S, Gunning, P, ...
Mutant human beta-actin genes were introduced into normal human (KD) fibroblasts and the derivative cell line HuT-12, which is immortalized but nontumorigenic, to test their ability to promote...
Ng, S Y, Gunning, P, Eddy, R, Ponte, P, Leavitt, J, Shows, T, ...
We have assigned six members of the human beta-actin multigene family to specific human chromosomes. The functional gene, ACTB, is located on human chromosome 7, and the other assigned...
Hickey, R, Skoultchi, A, Gunning, P, Kedes, L
The rat myogenic cell line L6E9 induces skeletal but not cardiac alpha-actin mRNA upon fusion to form myotubes. However, when a human cardiac alpha-actin gene was introduced into L6E9 myoblasts,...
Differential patterns of transcript accumulation during human myogenesis.
Gunning, P, Hardeman, E, Wade, R, Ponte, P, Bains, W, Blau, H M, ...
We evaluated the extent to which muscle-specific genes display identical patterns of mRNA accumulation during human myogenesis. Cloned satellite cells isolated from adult human skeletal muscle were...
Gunning, P, Ponte, P, Okayama, H, Engel, J, Blau, H, Kedes, L
cDNA clones encoding three classes of human actins have been isolated and characterized. The first two classes (gamma and beta, cytoplasmic actins) were obtained from a cDNA library constructed from...
Molecular cloning and characterization of mutant and wild-type human beta-actin genes.
Leavitt, J, Gunning, P, Porreca, P, Ng, S Y, Lin, C S, Kedes, L
There are more than 20 beta-actin-specific sequences in the human genome, many of which are pseudogenes. To facilitate the isolation of potentially functional beta-actin genes, we used the new method...
Ponte, P, Gunning, P, Blau, H, Kedes, L
We have constructed isotype-specific subclones from the 3' untranslated regions of alpha-skeletal, alpha-cardiac, beta-cytoskeletal, and gamma-cytoskeletal actin cDNAs. These clones have been used as...
Gunning, P, Ponte, P, Blau, H, Kedes, L
We determined the actin isotypes encoded by 30 actin cDNA clones previously isolated from an adult human muscle cDNA library. Using 3' untranslated region probes derived from alpha-skeletal, beta-...
Wade, R, Gunning, P, Eddy, R, Shows, T, Kedes, L
The carbonic anhydrases (CA) are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide. The genes for the carbonic anhydrase isozymes are members of a multigene family...
Lin, C S, Ng, S Y, Gunning, P, Kedes, L, Leavitt, J
We have sequenced the mutant beta-actin gene of a tumorigenic human fibroblast cell line (HuT-14T) and found that it carries three mutations that alter the amino acids at positions 36, 83, and 244 as...
Gunning, P, Leavitt, J, Muscat, G, Ng, S Y, Kedes, L
We have constructed a mammalian expression vector consisting of 3 kilobases of the human beta-actin gene 5' flanking sequence plus 5' untranslated region and intervening sequence 1 linked at the 3'...
Erba, H P, Gunning, P, Kedes, L
Two distinct, but iso-coding, gamma non-muscle actin cDNAs were isolated from an SV40-transformed human fibroblast library. The complete nucleotide sequence of the human gamma non-muscle actin cDNAs...
Ponte, P, Ng, S Y, Engel, J, Gunning, P, Kedes, L
We report the complete nucleotide sequence of a human beta actin cDNA. Both the 5' and 3' untranslated regions of the sequence are similar (greater than 80%) to the analogous regions of the rat...
Regulation of the human beta-actin promoter by upstream and intron domains.
Ng, S Y, Gunning, P, Liu, S H, Leavitt, J, Kedes, L
We have identified three regulatory domains of the complex human beta-actin gene promoter. They span a region of about 3000 bases, from not more than -2011 bases upstream of the mRNA cap site to...
Chromosomal location of the co-expressed human skeletal and cardiac actin genes.
Gunning, P, Ponte, P, Kedes, L, Eddy, R, Shows, T
We have examined the relationship between chromosomal location and regulation of the two human genes encoding the sarcomeric muscle actins. The human genes encoding skeletal alpha-actin and cardiac...
Gamma Tropomyosin Gene Products Are Required for Embryonic Development
Hook, J., Lemckert, F., Qin, H., Schevzov, G., Gunning, P.
The actin filament system is essential for many cellular functions, including shape, motility, cytokinesis, intracellular trafficking, and tissue organization. Tropomyosins (Tms) are rod-like...
Erba, H P, Eddy, R, Shows, T, Kedes, L, Gunning, P
The accumulation of the cytoskeletal beta- and gamma-actin mRNAs was determined in a variety of mouse tissues and organs. The beta-isoform is always expressed in excess of the gamma-isoform. However,...
Leavitt, J, Ng, S Y, Aebi, U, Varma, M, Latter, G, Burbeck, S, ...
Two different mutant human beta-actin genes have been introduced into normal diploid human (KD) fibroblasts and their immortalized derivative cell line, HuT-12, to assess the impact of an abnormal...
Expression of transfected mutant beta-actin genes: transitions toward the stable tumorigenic state.
Leavitt, J, Ng, S Y, Varma, M, Latter, G, Burbeck, S, Gunning, P, ...
Mutant human beta-actin genes were introduced into normal human (KD) fibroblasts and the derivative cell line HuT-12, which is immortalized but nontumorigenic, to test their ability to promote...
Ng, S Y, Gunning, P, Eddy, R, Ponte, P, Leavitt, J, Shows, T, ...
We have assigned six members of the human beta-actin multigene family to specific human chromosomes. The functional gene, ACTB, is located on human chromosome 7, and the other assigned...
Hickey, R, Skoultchi, A, Gunning, P, Kedes, L
The rat myogenic cell line L6E9 induces skeletal but not cardiac alpha-actin mRNA upon fusion to form myotubes. However, when a human cardiac alpha-actin gene was introduced into L6E9 myoblasts,...
Differential patterns of transcript accumulation during human myogenesis.
Gunning, P, Hardeman, E, Wade, R, Ponte, P, Bains, W, Blau, H M, ...
We evaluated the extent to which muscle-specific genes display identical patterns of mRNA accumulation during human myogenesis. Cloned satellite cells isolated from adult human skeletal muscle were...
Gunning, P, Ponte, P, Okayama, H, Engel, J, Blau, H, Kedes, L
cDNA clones encoding three classes of human actins have been isolated and characterized. The first two classes (gamma and beta, cytoplasmic actins) were obtained from a cDNA library constructed from...
Molecular cloning and characterization of mutant and wild-type human beta-actin genes.
Leavitt, J, Gunning, P, Porreca, P, Ng, S Y, Lin, C S, Kedes, L
There are more than 20 beta-actin-specific sequences in the human genome, many of which are pseudogenes. To facilitate the isolation of potentially functional beta-actin genes, we used the new method...
Ponte, P, Gunning, P, Blau, H, Kedes, L
We have constructed isotype-specific subclones from the 3' untranslated regions of alpha-skeletal, alpha-cardiac, beta-cytoskeletal, and gamma-cytoskeletal actin cDNAs. These clones have been used as...
Gunning, P, Ponte, P, Blau, H, Kedes, L
We determined the actin isotypes encoded by 30 actin cDNA clones previously isolated from an adult human muscle cDNA library. Using 3' untranslated region probes derived from alpha-skeletal, beta-...
Wade, R, Gunning, P, Eddy, R, Shows, T, Kedes, L
The carbonic anhydrases (CA) are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide. The genes for the carbonic anhydrase isozymes are members of a multigene family...
Lin, C S, Ng, S Y, Gunning, P, Kedes, L, Leavitt, J
We have sequenced the mutant beta-actin gene of a tumorigenic human fibroblast cell line (HuT-14T) and found that it carries three mutations that alter the amino acids at positions 36, 83, and 244 as...