P. H. Lohman

Publication List Details

Period

2008 - 2008

Number

33

Co-Authors

XR-C1, a new CHO cell mutant which is defective in DNA-PKcs, is impaired in both V(D)J coding and signal joint formation.

Errami, A, He, D M, Friedl, A A, Overkamp, W J, Morolli, B, Hendrickson, E A, ...

DNA-dependent protein kinase (DNA-PK) plays an important role in DNA double-strand break (DSB) repair and V(D)J recombination. We have isolated a new X-ray-sensitive CHO cell line, XR-C1, which is...

Molecular and biochemical characterization of new X-ray-sensitive hamster cell mutants defective in Ku80.

Errami, A, Finnie, N J, Morolli, B, Jackson, S P, Lohman, P H, Zdzienicka, M Z

Ku, a heterodimer of approximately 70 and approximately 80 kDa subunits, is a nuclear protein that binds to double-stranded DNA ends and is a component of the DNA-dependent protein kinase (DNA-PK)....

Determination of spontaneous loss of heterozygosity mutations in Aprt heterozygous mice.

Van Sloun, P P, Wijnhoven, S W, Kool, H J, Slater, R, Weeda, G, Van Zeeland, A A, ...

A mouse model was generated to investigate loss of heterozygosity (LOH) events in somatic cells. The adenine phosphoribosyltransferase ( Aprt ) gene was disrupted in embryonic stem cells using a...

Identification and characterisation of the Drosophila melanogaster O6-alkylguanine-DNA alkyltransferase cDNA.

Kooistra, R, Zonneveld, J B, Watson, A J, Margison, G P, Lohman, P H, Pastink, A

The protein O 6-alkylguanine-DNA alkyltransferase(alkyltransferase) is involved in the repair of O 6-alkylguanine and O 4-alkylthymine in DNA and plays an important role in most organisms in...

The role of nucleotide excision repair in protecting embryonic stem cells from genotoxic effects of UV-induced DNA damage.

Van Sloun, P P, Jansen, J G, Weeda, G, Mullenders, L H, Van Zeeland, A A, Lohman, P H, ...

In this study the role of nucleotide excision repair (NER) in protecting mouse embryonic stem (ES) cells against the genotoxic effects of UV-photolesions was analysed. Repair of cyclobutane...

The Drosophila melanogaster RAD54 homolog, DmRAD54, is involved in the repair of radiation damage and recombination.

Kooistra, R, Vreeken, K, Zonneveld, J B, De Jong, A, Eeken, J C, Osgood, C J, ...

The RAD54 gene of Saccharomyces cerevisiae plays a crucial role in recombinational repair of double-strand breaks in DNA. Here the isolation and functional characterization of the RAD54 homolog of...

Efficient rescue of integrated shuttle vectors from transgenic mice: a model for studying mutations in vivo.

Gossen, J A, De Leeuw, W J, Tan, C H, Zwarthoff, E C, Berends, F, Lohman, P H, ...

To study gene mutations in different organs and tissues of an experimental animal, we produced transgenic mice harboring bacteriophage lambda shuttle vectors integrated in the genome in a...

Cloning the RAD51 homologue of Schizosaccharomyces pombe.

Muris, D F, Vreeken, K, Carr, A M, Broughton, B C, Lehmann, A R, Lohman, P H, ...

The RAD51 gene of Saccharomyces cerevisiae encodes a RecA like protein, which is involved in the recombinational repair of double strand breaks. We have isolated the RAD51 homologue, rhp51+, of the...

Detection and quantification of adducts formed upon interaction of diamminedichloroplatinum (II) with DNA, by anion-exchange chromatography after enzymatic degradation.

Fichtinger-Schepman, A M, Lohman, P H, Reedijk, J

A method has been developed to determine the adducts formed upon interaction of cis- and trans-diamminedichloroplatinum(II) (cis- and trans-DDP) with DNA. After 5 h at 50 degrees C in the dark, the...

Repair of UV-induced pyrimidine dimers in the individual genes Gart, Notch and white from Drosophila melanogaster cell lines.

De Cock, J G, Klink, E C, Ferro, W, Lohman, P H, Eeken, J C

The excision repair of UV-induced pyrimidine dimers was investigated in three genes: Gart, Notch and white in a permanent Drosophila cell line Kc, derived from wild type Drosophila melanogaster...

Repair of UV-induced (6-4)photoproducts measured in individual genes in the Drosophila embryonic Kc cell line.

De Cock, J G, Van Hoffen, A, Wijnands, J, Molenaar, G, Lohman, P H, Eeken, J C

The nucleotide excision repair (NER; dark-repair) of (6-4)photoproducts ((6-4)PPs) was assayed in cells from a permanent Drosophila melanogaster embryonic cell line, Kc, after exposure to 20 or 40...

DNA strand specificity for UV-induced mutations in mammalian cells.

Vrieling, H, Van Rooijen, M L, Groen, N A, Zdzienicka, M Z, Simons, J W, Lohman, P H, ...

The influence of DNA repair on the molecular nature of mutations induced by UV light (254 nm) was investigated in UV-induced hprt mutants from UV-sensitive Chinese hamster cells (V-H1) and the...

Xeroderma pigmentosum cells with normal levels of excision repair have a defect in DNA synthesis after UV-irradiation.

Lehman, A R, Kirk-Bell, S, Arlett, C F, Paterson, M C, Lohman, P H, De Weerd-Kastelein, E A, ...

Cells cultured from most patients suffering from the sunlight-sensitive hereditary disorder xeroderma pigmentosum are defective in the ability to excise ultraviolet light (UV)-induced pyrimidine...

Use of monoclonal and polyclonal antibodies against DNA adducts for the detection of DNA lesions in isolated DNA and in single cells.

Baan, R A, Zaalberg, O B, Fichtinger-Schepman, A M, Muysken-Schoen, M A, Lansbergen, M J, Lohman, P H

Interaction of genotoxic chemicals with their intracellular target, i.e., DNA, may result in the formation of covalent adducts. Various methods have been developed to estimate exposure to genotoxic...

XR-C1, a new CHO cell mutant which is defective in DNA-PKcs, is impaired in both V(D)J coding and signal joint formation.

Errami, A, He, D M, Friedl, A A, Overkamp, W J, Morolli, B, Hendrickson, E A, ...

DNA-dependent protein kinase (DNA-PK) plays an important role in DNA double-strand break (DSB) repair and V(D)J recombination. We have isolated a new X-ray-sensitive CHO cell line, XR-C1, which is...

Molecular and biochemical characterization of new X-ray-sensitive hamster cell mutants defective in Ku80.

Errami, A, Finnie, N J, Morolli, B, Jackson, S P, Lohman, P H, Zdzienicka, M Z

Ku, a heterodimer of approximately 70 and approximately 80 kDa subunits, is a nuclear protein that binds to double-stranded DNA ends and is a component of the DNA-dependent protein kinase (DNA-PK)....

Determination of spontaneous loss of heterozygosity mutations in Aprt heterozygous mice.

Van Sloun, P P, Wijnhoven, S W, Kool, H J, Slater, R, Weeda, G, Van Zeeland, A A, ...

A mouse model was generated to investigate loss of heterozygosity (LOH) events in somatic cells. The adenine phosphoribosyltransferase ( Aprt ) gene was disrupted in embryonic stem cells using a...

Identification and characterisation of the Drosophila melanogaster O6-alkylguanine-DNA alkyltransferase cDNA.

Kooistra, R, Zonneveld, J B, Watson, A J, Margison, G P, Lohman, P H, Pastink, A

The protein O 6-alkylguanine-DNA alkyltransferase(alkyltransferase) is involved in the repair of O 6-alkylguanine and O 4-alkylthymine in DNA and plays an important role in most organisms in...

The role of nucleotide excision repair in protecting embryonic stem cells from genotoxic effects of UV-induced DNA damage.

Van Sloun, P P, Jansen, J G, Weeda, G, Mullenders, L H, Van Zeeland, A A, Lohman, P H, ...

In this study the role of nucleotide excision repair (NER) in protecting mouse embryonic stem (ES) cells against the genotoxic effects of UV-photolesions was analysed. Repair of cyclobutane...

The Drosophila melanogaster RAD54 homolog, DmRAD54, is involved in the repair of radiation damage and recombination.

Kooistra, R, Vreeken, K, Zonneveld, J B, De Jong, A, Eeken, J C, Osgood, C J, ...

The RAD54 gene of Saccharomyces cerevisiae plays a crucial role in recombinational repair of double-strand breaks in DNA. Here the isolation and functional characterization of the RAD54 homolog of...

Efficient rescue of integrated shuttle vectors from transgenic mice: a model for studying mutations in vivo.

Gossen, J A, De Leeuw, W J, Tan, C H, Zwarthoff, E C, Berends, F, Lohman, P H, ...

To study gene mutations in different organs and tissues of an experimental animal, we produced transgenic mice harboring bacteriophage lambda shuttle vectors integrated in the genome in a...

Cloning the RAD51 homologue of Schizosaccharomyces pombe.

Muris, D F, Vreeken, K, Carr, A M, Broughton, B C, Lehmann, A R, Lohman, P H, ...

The RAD51 gene of Saccharomyces cerevisiae encodes a RecA like protein, which is involved in the recombinational repair of double strand breaks. We have isolated the RAD51 homologue, rhp51+, of the...

Detection and quantification of adducts formed upon interaction of diamminedichloroplatinum (II) with DNA, by anion-exchange chromatography after enzymatic degradation.

Fichtinger-Schepman, A M, Lohman, P H, Reedijk, J

A method has been developed to determine the adducts formed upon interaction of cis- and trans-diamminedichloroplatinum(II) (cis- and trans-DDP) with DNA. After 5 h at 50 degrees C in the dark, the...

Repair of UV-induced pyrimidine dimers in the individual genes Gart, Notch and white from Drosophila melanogaster cell lines.

De Cock, J G, Klink, E C, Ferro, W, Lohman, P H, Eeken, J C

The excision repair of UV-induced pyrimidine dimers was investigated in three genes: Gart, Notch and white in a permanent Drosophila cell line Kc, derived from wild type Drosophila melanogaster...

Repair of UV-induced (6-4)photoproducts measured in individual genes in the Drosophila embryonic Kc cell line.

De Cock, J G, Van Hoffen, A, Wijnands, J, Molenaar, G, Lohman, P H, Eeken, J C

The nucleotide excision repair (NER; dark-repair) of (6-4)photoproducts ((6-4)PPs) was assayed in cells from a permanent Drosophila melanogaster embryonic cell line, Kc, after exposure to 20 or 40...

DNA strand specificity for UV-induced mutations in mammalian cells.

Vrieling, H, Van Rooijen, M L, Groen, N A, Zdzienicka, M Z, Simons, J W, Lohman, P H, ...

The influence of DNA repair on the molecular nature of mutations induced by UV light (254 nm) was investigated in UV-induced hprt mutants from UV-sensitive Chinese hamster cells (V-H1) and the...

Xeroderma pigmentosum cells with normal levels of excision repair have a defect in DNA synthesis after UV-irradiation.

Lehman, A R, Kirk-Bell, S, Arlett, C F, Paterson, M C, Lohman, P H, De Weerd-Kastelein, E A, ...

Cells cultured from most patients suffering from the sunlight-sensitive hereditary disorder xeroderma pigmentosum are defective in the ability to excise ultraviolet light (UV)-induced pyrimidine...

Use of monoclonal and polyclonal antibodies against DNA adducts for the detection of DNA lesions in isolated DNA and in single cells.

Baan, R A, Zaalberg, O B, Fichtinger-Schepman, A M, Muysken-Schoen, M A, Lansbergen, M J, Lohman, P H

Interaction of genotoxic chemicals with their intracellular target, i.e., DNA, may result in the formation of covalent adducts. Various methods have been developed to estimate exposure to genotoxic...

Studies on phenotypic complementation of ataxia-telangiectasia cells by chromosome transfer.

Jongmans, W, Verhaegh, G W, Jaspers, N G, Oshimura, M, Stanbridge, E J, Lohman, P H, ...

Cells derived from patients with the cancer-prone inherited disorder ataxia-telangiectasia (A-T) show an abnormal response to ionizing radiation-induced DNA damage, such as an increased cell killing...

A gene that regulates DNA replication in response to DNA damage is located on human chromosome 4q.

Verhaegh, G W, Jongmans, W, Jaspers, N G, Natarajan, A T, Oshimura, M, Lohman, P H, ...

Inhibition of replicative DNA synthesis following gamma-irradiation is observed in eukaryotic cells but is defective in cells derived from patients with the cancer-prone inherited disorder...