Antibacterial activity and mechanism of action of 3'-azido-3'-deoxythymidine (BW A509U).
Elwell, L P, Ferone, R, Freeman, G A, Fyfe, J A, Hill, J A, Ray, P H, ...
The thymidine analog 3'-azido-3'-deoxythymidine (BW A509U; azidothymidine [AZT]) had potent bactericidal activity against many members of the family Enterobacteriaceae, including strains of...
Dallas, W S, Gowen, J E, Ray, P H, Cox, M J, Dev, I K
The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased...
Talarico, T L, Ray, P H, Dev, I K, Merrill, B M, Dallas, W S
The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids,...
Talarico, T L, Dev, I K, Dallas, W S, Ferone, R, Ray, P H
The enzymes 7,8-dihydroxymethylpterin-pyrophosphokinase (HPPK) and 7,8-dihydropteroate synthase (DHPS), which act sequentially in the folate pathway, were purified to homogeneity from crude extracts...
Tian, G, Wu, H C, Ray, P H, Tai, P C
The requirements for the translocation of prolipoprotein into membrane vesicles were examined in an in vitro system. As measured by the eventual modification and processing of the prolipoprotein to...
Rasmussen, B A, MacGregor, C H, Ray, P H, Bassford, P J
It has not been possible to obtain in vitro expression of the positively regulated malE gene encoding the periplasmic maltose-binding protein (MBP) of Escherichia coli. To facilitate in vitro malE...
Ray, P H, Benedict, C D, Grasmuk, H
Cytidine 5'-triphosphate:cytidine 5'-monophosphate-3-deoxy-D-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) was purified 2,300-fold from frozen Escherichia coli B cells. The enzyme...
Dallas, W S, Ray, P H, Leong, J, Benedict, C D, Stamm, L V, Bassford, P J
A recombinant plasmid designated pLVS3 previously was described that harbored a 14-kilobase insert of Treponema pallidum genomic DNA. Escherichia coli maxicells programmed with this plasmid...
Weiss, J B, Ray, P H, Bassford, P J
The efficient export of a subset of Escherichia coli envelope proteins is dependent upon the product of the secB gene. Previous studies indicated that SecB promotes the export of the periplasmic...
3-Deoxy-D-manno-octulosonate (KDO)-8-phosphate synthetase has been purified 450-fold from frozen Escherichia coli B cells. The purified enzyme catalyzed the stoichiometric formation of...
Antibacterial activity and mechanism of action of 3'-azido-3'-deoxythymidine (BW A509U).
Elwell, L P, Ferone, R, Freeman, G A, Fyfe, J A, Hill, J A, Ray, P H, ...
The thymidine analog 3'-azido-3'-deoxythymidine (BW A509U; azidothymidine [AZT]) had potent bactericidal activity against many members of the family Enterobacteriaceae, including strains of...
Dallas, W S, Gowen, J E, Ray, P H, Cox, M J, Dev, I K
The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased...
Talarico, T L, Ray, P H, Dev, I K, Merrill, B M, Dallas, W S
The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids,...
Talarico, T L, Dev, I K, Dallas, W S, Ferone, R, Ray, P H
The enzymes 7,8-dihydroxymethylpterin-pyrophosphokinase (HPPK) and 7,8-dihydropteroate synthase (DHPS), which act sequentially in the folate pathway, were purified to homogeneity from crude extracts...
Tian, G, Wu, H C, Ray, P H, Tai, P C
The requirements for the translocation of prolipoprotein into membrane vesicles were examined in an in vitro system. As measured by the eventual modification and processing of the prolipoprotein to...
Rasmussen, B A, MacGregor, C H, Ray, P H, Bassford, P J
It has not been possible to obtain in vitro expression of the positively regulated malE gene encoding the periplasmic maltose-binding protein (MBP) of Escherichia coli. To facilitate in vitro malE...
Ray, P H, Benedict, C D, Grasmuk, H
Cytidine 5'-triphosphate:cytidine 5'-monophosphate-3-deoxy-D-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) was purified 2,300-fold from frozen Escherichia coli B cells. The enzyme...
Dallas, W S, Ray, P H, Leong, J, Benedict, C D, Stamm, L V, Bassford, P J
A recombinant plasmid designated pLVS3 previously was described that harbored a 14-kilobase insert of Treponema pallidum genomic DNA. Escherichia coli maxicells programmed with this plasmid...
Weiss, J B, Ray, P H, Bassford, P J
The efficient export of a subset of Escherichia coli envelope proteins is dependent upon the product of the secB gene. Previous studies indicated that SecB promotes the export of the periplasmic...
3-Deoxy-D-manno-octulosonate (KDO)-8-phosphate synthetase has been purified 450-fold from frozen Escherichia coli B cells. The purified enzyme catalyzed the stoichiometric formation of...