P. J. Barker

Publication List Details

Period

1998 - 2005

Number

23

Co-Authors

Stress signaling in plants: a mitogen-activated protein kinase pathway is activated by cold and drought.

Jonak, C, Kiegerl, S, Ligterink, W, Barker, P J, Huskisson, N S, Hirt, H

Yeast and animals use mitogen-activated protein (MAP) kinase cascades to mediate stress and extracellular signals. We have tested whether MAP kinases are involved in mediating environmental stress...

Cloning of a Streptomyces clavuligerus DNA fragment encoding the cephalosporin 7 alpha-hydroxylase and its expression in Streptomyces lividans.

Xiao, X, Hintermann, G, Häusler, A, Barker, P J, Foor, F, Demain, A L, ...

A 26-mer DNA probe was designed from N-terminal sequence data for the cephalosporin 7 alpha-hydroxylase (CH) of Streptomyces clavuligerus NRRL 3585 and used to screen a DNA library from this...

Purification and characterization of a new endoglucanase from Clostridium thermocellum.

Romaniec, M P, Fauth, U, Kobayashi, T, Huskisson, N S, Barker, P J, Demain, A L

An endoglucanase (1,4-beta-D-glucan glucanohydrolase, EC 3.2.1.4) from the thermophilic anaerobe Clostridium thermocellum was purified to apparent homogeneity without the use of denaturants. No...

Purification and characterization of heparin-binding growth factors from porcine uterus.

Brigstock, D R, Heap, R B, Barker, P J, Brown, K D

Heparin-binding growth factors present in pig uterine tissue were purified by approx. 50,000-fold using a combination of ammonium sulphate precipitation, ion-exchange chromatography and...

A mammalian epididymal protein with remarkable sequence similarity to snake venom haemorrhagic peptides.

Perry, A C, Jones, R, Barker, P J, Hall, L

Following spermatogenesis in the testis, mammalian spermatozoa pass into the epididymis, where they undergo changes which confer on them forward motility and the ability to recognize and penetrate...

Binding of gelatinases A and B to type-I collagen and other matrix components.

Allan, J A, Docherty, A J, Barker, P J, Huskisson, N S, Reynolds, J J, Murphy, G

Matrix sequestration of matrix metalloproteinases may be important for the facilitation of remodelling events and the migration of cells through the extracellular matrix. Using an ELISA technique we...

The availability of carnitine acetyltransferase in mitochondria from guinea-pig liver and other tissues

Barker, P. J., Fincham, N. J., Hardwick, D. C.

The carnitine acetyltransferase and glutamate dehydrogenase activities of guinea-pig liver and other tissues were estimated. Both enzymes are wholly mitochondrial, and can only be fully observed...

The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains.

Ferreira, L M, Hazlewood, G P, Barker, P J, Gilbert, H J

A genomic library of Pseudomonas fluorescens subsp. cellulosa DNA was constructed in pUC18 and Escherichia coli recombinants expressing 4-methylumbelliferyl beta-D-cellobioside-hydrolysing activity...

Stress signaling in plants: a mitogen-activated protein kinase pathway is activated by cold and drought.

Jonak, C, Kiegerl, S, Ligterink, W, Barker, P J, Huskisson, N S, Hirt, H

Yeast and animals use mitogen-activated protein (MAP) kinase cascades to mediate stress and extracellular signals. We have tested whether MAP kinases are involved in mediating environmental stress...

Cloning of a Streptomyces clavuligerus DNA fragment encoding the cephalosporin 7 alpha-hydroxylase and its expression in Streptomyces lividans.

Xiao, X, Hintermann, G, Häusler, A, Barker, P J, Foor, F, Demain, A L, ...

A 26-mer DNA probe was designed from N-terminal sequence data for the cephalosporin 7 alpha-hydroxylase (CH) of Streptomyces clavuligerus NRRL 3585 and used to screen a DNA library from this...

Purification and characterization of a new endoglucanase from Clostridium thermocellum.

Romaniec, M P, Fauth, U, Kobayashi, T, Huskisson, N S, Barker, P J, Demain, A L

An endoglucanase (1,4-beta-D-glucan glucanohydrolase, EC 3.2.1.4) from the thermophilic anaerobe Clostridium thermocellum was purified to apparent homogeneity without the use of denaturants. No...

Purification and characterization of heparin-binding growth factors from porcine uterus.

Brigstock, D R, Heap, R B, Barker, P J, Brown, K D

Heparin-binding growth factors present in pig uterine tissue were purified by approx. 50,000-fold using a combination of ammonium sulphate precipitation, ion-exchange chromatography and...

A mammalian epididymal protein with remarkable sequence similarity to snake venom haemorrhagic peptides.

Perry, A C, Jones, R, Barker, P J, Hall, L

Following spermatogenesis in the testis, mammalian spermatozoa pass into the epididymis, where they undergo changes which confer on them forward motility and the ability to recognize and penetrate...

Binding of gelatinases A and B to type-I collagen and other matrix components.

Allan, J A, Docherty, A J, Barker, P J, Huskisson, N S, Reynolds, J J, Murphy, G

Matrix sequestration of matrix metalloproteinases may be important for the facilitation of remodelling events and the migration of cells through the extracellular matrix. Using an ELISA technique we...

The cellodextrinase from Pseudomonas fluorescens subsp. cellulosa consists of multiple functional domains.

Ferreira, L M, Hazlewood, G P, Barker, P J, Gilbert, H J

A genomic library of Pseudomonas fluorescens subsp. cellulosa DNA was constructed in pUC18 and Escherichia coli recombinants expressing 4-methylumbelliferyl beta-D-cellobioside-hydrolysing activity...

The availability of carnitine acetyltransferase in mitochondria from guinea-pig liver and other tissues

Barker, P. J., Fincham, N. J., Hardwick, D. C.

The carnitine acetyltransferase and glutamate dehydrogenase activities of guinea-pig liver and other tissues were estimated. Both enzymes are wholly mitochondrial, and can only be fully observed...

Blot-sequencing of antibodies: application to analysis of V gene usage among anti-steroid monoclonal antibodies

Taussig, M. J., Symington, P. R., Woods, M., Sims, M. J., Beale, D., Humphreys, A. S., ...

Automated gas-phase protein sequencing has been used to characterize variable regions of antibody heavy and light chains separated by SDS—polyacrylamide gel electrophoresis (PAGE) and...

An anti-peptide antibody that recognizes a neo-antigen in the CR1 stump remaining on erythrocytes after proteolysis.

Barbosa, J E, Harrison, R A, Barker, P J, Lachmann, P J

Previous studies of erythrocyte CR1 levels in systemic lupus erythematosus (SLE) and other diseases with in vivo complement activation have led to the conclusion that CR1 levels fall because of loss...