Chary, V K, De La Fuente, J L, Liras, P, Martin, J F
Promoter probe vectors containing the pA origin of replication and the Streptomyces griseus promoterless amy gene (encoding alpha-amylase) as reporter have been constructed to study transcription...
Coque, J J, Enguita, F J, Martín, J F, Liras, P
Two genes, cmcI and cmcJ, corresponding to open reading frames 7 and 8 (ORF7 and ORF8) of the cephamycin C cluster of Nocardia lactamdurans encode enzymes that convert cephalosporin C to...
Pérez-Llarena, F J, Liras, P, Rodríguez-García, A, Martín, J F
A regulatory gene (ccaR), located within the cephamycin gene cluster of Streptomyces clavuligerus, is linked to a gene (blp) encoding a protein similar to a beta-lactamase-inhibitory protein....
Pérez-Llarena, F, Martín, J F, Galleni, M, Coque, J J, Fuente, J L, Frère, J M, ...
A gene (bla) encoding a beta-lactamase is present in the cephamycin gene cluster of Streptomyces clavuligerus, the strain producing clavulanic acid and a beta-lactamase inhibitory protein. The bla...
Efficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus.
Garcia-Dominguez, M, Martin, J F, Mahro, B, Demain, A L, Liras, P
The conditions for optimal formation and regeneration of protoplasts of Streptomyces clavuligerus were established. The optimal temperature for regeneration of protoplasts and for transformation was...
Ludovice, M, Martin, J F, Carrachas, P, Liras, P
The argC gene of Streptomyces clavuligerus encoding N-acetylglutamyl-phosphate reductase (AGPR) has been cloned by complementation of argC mutants Streptomyces lividans 1674 and Escherichia coli...
Garcia-Gonzalez, M D, Martin, J F, Vigal, T, Liras, P
Extracellular amylase in Streptomyces lividans was undetectable in starch-supplemented medium. However, S. lividans produced fivefold-higher levels of amylase than Streptomyces griseus IMRU 3570 when...
Coque, J J, Liras, P, Laiz, L, Martín, J F
A gene (lat) encoding lysine 6-aminotransferase was found upstream of the pcbAB (encoding alpha-aminoadipylcysteinyl-valine synthetase) and pcbC (encoding isopenicillin N synthase) genes in the...
Garcia-Dominguez, M, Martin, J F, Liras, P
Wild-type Streptomyces clavuligerus NRRL 3585 is unable to utilize glucose. A glucose-utilizing (gut-1) mutant of S. clavuligerus NRRL 3585 has been obtained by N-methyl-N'-nitro-N-nitrosoguanidine...
Utilization of ornithine and arginine as specific precursors of clavulanic acid.
Romero, J, Liras, P, Martín, J F
Ornithine and arginine (5 to 20 mM), but not glutamic acid or proline, exerted a concentration-dependent stimulatory effect on the biosynthesis of clavulanic acid in both resting-cell cultures and...
García-Domínguez, M, Liras, P, Martín, J F
A gene, pcbC, encoding the isopenicillin N synthase of Streptomyces griseus NRRL 3851, has been cloned in a 6.4-kb Bg/II DNA fragment and located in an internal 1.55-kb PvuII segment by hybridization...
Coque, J J, Liras, P, Martín, J F
Three genes encoding a typical beta-lactamase, a penicillin-binding protein (PBP4) and a transmembrane protein are located in the cluster of cephamycin biosynthetic genes in Nocardia lactamdurans....
De La Fuente, A., Martín, J. F., Rodríguez-García, A., Liras, P.
The oat2 gene, located in the clavulanic acid gene cluster in Streptomyces clavuligerus, is similar to argJ, which encodes N-acetylornithine:glutamic acid acetyltransferase activity. Purified...
De La Fuente, J L, Martin, J F, Liras, P
The ornithine carbamoyltransferases (OTCases) from the beta-lactam-producing actinomycetes Streptomyces clavuligerus and Nocardia lactamdurans have been purified to near-homogeneity by...
De La Fuente, J L, Rumbero, A, Martín, J F, Liras, P
Delta-1-Piperideine-6-carboxylate (P6C) dehydrogenase activity, which catalyses the conversion of P6C into alpha-aminoadipic acid, has been studied in the cephamycin C producer Streptomyces...
Chary, V K, De La Fuente, J L, Liras, P, Martin, J F
Promoter probe vectors containing the pA origin of replication and the Streptomyces griseus promoterless amy gene (encoding alpha-amylase) as reporter have been constructed to study transcription...
Coque, J J, Enguita, F J, Martín, J F, Liras, P
Two genes, cmcI and cmcJ, corresponding to open reading frames 7 and 8 (ORF7 and ORF8) of the cephamycin C cluster of Nocardia lactamdurans encode enzymes that convert cephalosporin C to...
Pérez-Llarena, F J, Liras, P, Rodríguez-García, A, Martín, J F
A regulatory gene (ccaR), located within the cephamycin gene cluster of Streptomyces clavuligerus, is linked to a gene (blp) encoding a protein similar to a beta-lactamase-inhibitory protein....
Pérez-Llarena, F, Martín, J F, Galleni, M, Coque, J J, Fuente, J L, Frère, J M, ...
A gene (bla) encoding a beta-lactamase is present in the cephamycin gene cluster of Streptomyces clavuligerus, the strain producing clavulanic acid and a beta-lactamase inhibitory protein. The bla...
Efficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus.
Garcia-Dominguez, M, Martin, J F, Mahro, B, Demain, A L, Liras, P
The conditions for optimal formation and regeneration of protoplasts of Streptomyces clavuligerus were established. The optimal temperature for regeneration of protoplasts and for transformation was...
Ludovice, M, Martin, J F, Carrachas, P, Liras, P
The argC gene of Streptomyces clavuligerus encoding N-acetylglutamyl-phosphate reductase (AGPR) has been cloned by complementation of argC mutants Streptomyces lividans 1674 and Escherichia coli...
Garcia-Gonzalez, M D, Martin, J F, Vigal, T, Liras, P
Extracellular amylase in Streptomyces lividans was undetectable in starch-supplemented medium. However, S. lividans produced fivefold-higher levels of amylase than Streptomyces griseus IMRU 3570 when...
Coque, J J, Liras, P, Laiz, L, Martín, J F
A gene (lat) encoding lysine 6-aminotransferase was found upstream of the pcbAB (encoding alpha-aminoadipylcysteinyl-valine synthetase) and pcbC (encoding isopenicillin N synthase) genes in the...
Garcia-Dominguez, M, Martin, J F, Liras, P
Wild-type Streptomyces clavuligerus NRRL 3585 is unable to utilize glucose. A glucose-utilizing (gut-1) mutant of S. clavuligerus NRRL 3585 has been obtained by N-methyl-N'-nitro-N-nitrosoguanidine...
Utilization of ornithine and arginine as specific precursors of clavulanic acid.
Romero, J, Liras, P, Martín, J F
Ornithine and arginine (5 to 20 mM), but not glutamic acid or proline, exerted a concentration-dependent stimulatory effect on the biosynthesis of clavulanic acid in both resting-cell cultures and...
García-Domínguez, M, Liras, P, Martín, J F
A gene, pcbC, encoding the isopenicillin N synthase of Streptomyces griseus NRRL 3851, has been cloned in a 6.4-kb Bg/II DNA fragment and located in an internal 1.55-kb PvuII segment by hybridization...
Coque, J J, Liras, P, Martín, J F
Three genes encoding a typical beta-lactamase, a penicillin-binding protein (PBP4) and a transmembrane protein are located in the cluster of cephamycin biosynthetic genes in Nocardia lactamdurans....
De La Fuente, A., Martín, J. F., Rodríguez-García, A., Liras, P.
The oat2 gene, located in the clavulanic acid gene cluster in Streptomyces clavuligerus, is similar to argJ, which encodes N-acetylornithine:glutamic acid acetyltransferase activity. Purified...
De La Fuente, J L, Martin, J F, Liras, P
The ornithine carbamoyltransferases (OTCases) from the beta-lactam-producing actinomycetes Streptomyces clavuligerus and Nocardia lactamdurans have been purified to near-homogeneity by...
De La Fuente, J L, Rumbero, A, Martín, J F, Liras, P
Delta-1-Piperideine-6-carboxylate (P6C) dehydrogenase activity, which catalyses the conversion of P6C into alpha-aminoadipic acid, has been studied in the cephamycin C producer Streptomyces...