Structural insights into the catalytic mechanism of trypanosoma cruzi trans -sialidase (2004)
Amaya, M. F., Watts, A. G., Damager, I., Wehenkel, A., Nguyen, T., Buschiazzo, A., ...
Neutron diffraction analysis of barium nitroprusside trihydrate at room temperature. (1991)
Navaza, A., Schweiss, P., Alzari, P.M., Chevrier, G., Heger, G., Guida, J.A.
Journal of Solid State Chemistry, 89(1990) S.23-30
Villeneuve, S., Souchon, H., Glaudemans, C. P. J., ...
The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex...
Hoog, S S, Pawlowski, J E, Alzari, P M, Penning, T M, Lewis, M
The 3.0-A-resolution x-ray structure of rat liver 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase (3 alpha-HSD, EC 1.1.1.50) was determined by molecular replacement using human...
Three-dimensional structure of a heteroclitic antigen-antibody cross-reaction complex.
Chitarra, V, Alzari, P M, Bentley, G A, Bhat, T N, Eiselé, J L, Houdusse, A, ...
Although antibodies are highly specific, cross-reactions are frequently observed. To understand the molecular basis of this phenomenon, we studied the anti-hen egg lysozyme (HEL) monoclonal antibody...
Lascombe, M B, Alzari, P M, Poljak, R J, Nisonoff, A
The three-dimensional structure of FabR19.9 from a well-characterized anti-p-azobenzenearsonate monoclonal antibody has been determined by x-ray diffraction techniques in two crystalline forms (I and...
The Wu-Kabat variability coefficient is a well-established descriptor of the susceptibility of an amino acid position to evolutionary replacements. It conveniently highlights stretches of accentuated...
Lascombe, M B, Alzari, P M, Boulot, G, Saludjian, P, Tougard, P, Berek, C, ...
The crystal structure of Fab R19.9, derived from an anti-p-azobenzenearsonate monoclonal antibody, has been determined and refined to 2.8-A resolution by x-ray crystallographic techniques. Monoclonal...
Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein.
Brunel, F, Alzari, P M, Ferrara, P, Zakin, M M
In the human transferrin gene promoter, PRI and DRI are positive cis-acting elements interacting respectively with two families of proteins, Tf-LF1 and Tf-LF2. In this paper, we report the...
Alzari, P M, Spinelli, S, Mariuzza, R A, Boulot, G, Poljak, R J, Jarvis, J M, ...
The three-dimensional structure of the Fab fragment of an anti-2-phenyloxazolone monoclonal antibody (NQ10/12.5) in its native and complexed forms has been determined at 2.8 and 3.0 A resolution,...
Structural basis of sialyltransferase activity in trypanosomal sialidases.
Buschiazzo, A, Tavares, G A, Campetella, O, Spinelli, S, Cremona, M L, París, G, ...
The intracellular parasite Trypanosoma cruzi, the etiological agent of Chagas disease, sheds a developmentally regulated surface trans-sialidase, which is involved in key aspects of parasite-host...
Saint-Joanis, B, Souchon, H, Wilming, M, Johnsson, K, Alzari, P M, Cole, S T
A series of mutants bearing single amino acid substitutions often encountered in the catalase/peroxidase, KatG, from isoniazid-resistant isolates of Mycobacterium tuberculosis has been produced by...
Villeneuve, S., Souchon, H., Glaudemans, C. P. J., ...
The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex...
Hoog, S S, Pawlowski, J E, Alzari, P M, Penning, T M, Lewis, M
The 3.0-A-resolution x-ray structure of rat liver 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase (3 alpha-HSD, EC 1.1.1.50) was determined by molecular replacement using human...
Three-dimensional structure of a heteroclitic antigen-antibody cross-reaction complex.
Chitarra, V, Alzari, P M, Bentley, G A, Bhat, T N, Eiselé, J L, Houdusse, A, ...
Although antibodies are highly specific, cross-reactions are frequently observed. To understand the molecular basis of this phenomenon, we studied the anti-hen egg lysozyme (HEL) monoclonal antibody...
Lascombe, M B, Alzari, P M, Poljak, R J, Nisonoff, A
The three-dimensional structure of FabR19.9 from a well-characterized anti-p-azobenzenearsonate monoclonal antibody has been determined by x-ray diffraction techniques in two crystalline forms (I and...
The Wu-Kabat variability coefficient is a well-established descriptor of the susceptibility of an amino acid position to evolutionary replacements. It conveniently highlights stretches of accentuated...
Lascombe, M B, Alzari, P M, Boulot, G, Saludjian, P, Tougard, P, Berek, C, ...
The crystal structure of Fab R19.9, derived from an anti-p-azobenzenearsonate monoclonal antibody, has been determined and refined to 2.8-A resolution by x-ray crystallographic techniques. Monoclonal...
Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein.
Brunel, F, Alzari, P M, Ferrara, P, Zakin, M M
In the human transferrin gene promoter, PRI and DRI are positive cis-acting elements interacting respectively with two families of proteins, Tf-LF1 and Tf-LF2. In this paper, we report the...
Alzari, P M, Spinelli, S, Mariuzza, R A, Boulot, G, Poljak, R J, Jarvis, J M, ...
The three-dimensional structure of the Fab fragment of an anti-2-phenyloxazolone monoclonal antibody (NQ10/12.5) in its native and complexed forms has been determined at 2.8 and 3.0 A resolution,...
Structural basis of sialyltransferase activity in trypanosomal sialidases.
Buschiazzo, A, Tavares, G A, Campetella, O, Spinelli, S, Cremona, M L, París, G, ...
The intracellular parasite Trypanosoma cruzi, the etiological agent of Chagas disease, sheds a developmentally regulated surface trans-sialidase, which is involved in key aspects of parasite-host...
Saint-Joanis, B, Souchon, H, Wilming, M, Johnsson, K, Alzari, P M, Cole, S T
A series of mutants bearing single amino acid substitutions often encountered in the catalase/peroxidase, KatG, from isoniazid-resistant isolates of Mycobacterium tuberculosis has been produced by...
Crystal structures of pheasant and guinea fowl egg-white lysozymes.
Lescar, J., Souchon, H., Alzari, P. M.
The crystal structures of pheasant and guinea fowl lysozymes have been determined by X-ray diffraction methods. Guinea fowl lysozyme crystallizes in space group P6(1)22 with cell dimensions a = 89.2...
Assemat, K., Alzari, P. M., Clément-Métral, J.
The internal residue Phe 25 in Rhodobacter sphaeroides thioredoxin was changed to five amino acids (Ala, Val, Leu, Ile, Tyr) by site-directed mutagenesis, and the mutant proteins were characterized...
Béguin, P., Raynaud, O., Chaveroche, M. K., Dridi, A., Alzari, P. M.
An Escherichia coli clone encoding a single cohesin domain of the cellulosome-integrating protein CipA from Clostridium thermocellum was constructed, and the corresponding polypeptide was purified,...
Crystallization and preliminary structural analysis of catalase A from Saccharomyces cerevisiae.
Berthet, S., Nykyri, L. M., Bravo, J., Mate, M. J., Berthet-Colominas, C., Alzari, P. M., ...
Yeast peroxisomal catalase A, obtained at high yields by over expression of the C-terminally modified gene from a 2 mu-plasmid, has been crystallized in a form suitable for high resolution X-ray...
Marina, A., Alzari, P. M., Bravo, J., Uriarte, M., Barcelona, B., Fita, I., ...
The enzymes carbamoyl phosphate synthetase (CPS) and carbamate kinase (CK) make carbamoyl phosphate in the same way: by ATP-phosphorylation of carbamate. The carbamate used by CK is made chemically,...