Effects of abasic sites and DNA single-strand breaks on prokaryotic RNA polymerases.
Abasic sites are thought to be the most frequently occurring cellular DNA damage and are generated spontaneously or as the result of chemical or radiation damage to DNA. In contrast to the wealth of...
Lenz, J, Okenquist, S A, LoSardo, J E, Hamilton, K K, Doetsch, P W
Damage to DNA can have lethal or mutagenic consequences for cells unless it is detected and repaired by cellular proteins. Repair depends on the ability of cellular factors to distinguish the damaged...
Dihydrouracil (DHU) is a DNA base damage product produced in significant amounts by ionizing radiation damage to cytosine under anoxic conditions. DHU represents a model for pyrimidine base damage...
Characterization of Schizosaccharomyces pombe Rad2 protein, a FEN-1 homolog.
FEN-1 proteins are a family of nucleases essential for lagging strand DNA synthesis. A gene with sequence similarity to FEN-1 protein-encoding genes, rad2 +, has been identified in...
Sentürker, S, Auffret Van Der Kemp, P, You, H J, Doetsch, P W, Dizdaroglu, M, Boiteux, S
Two genes of Saccharomyces cerevisiae, NTG1 and NTG2, encode proteins with a significant sequence homology to the endonuclease III of Escherichia coli. The Ntg1 and Ntg2 proteins were overexpressed...
Substrate specificity of ultraviolet DNA endonuclease (UVDE/Uve1p) from Schizosaccharomyces pombe.
Avery, A M, Kaur, B, Taylor, J S, Mello, J A, Essigmann, J M, Doetsch, P W
Schizosaccharomyces pombe ultraviolet DNA endonuclease (UVDE or Uve1p) has been shown to cleave 5' to UV light-induced cyclobutane pyrimidine dimers (CPDs) and pyrimidine-pyrimidone (6-4)...
An alternative eukaryotic DNA excision repair pathway.
Freyer, G A, Davey, S, Ferrer, J V, Martin, A M, Beach, D, Doetsch, P W
DNA lesions induced by UV light, cyclobutane pyrimidine dimers, and (6-4)pyrimidine pyrimidones are known to be repaired by the process of nucleotide excision repair (NER). However, in the fission...
RNA polymerase bypass at sites of dihydrouracil: implications for transcriptional mutagenesis.
Dihydrouracil (DHU) is a major base damage product formed from cytosine following exposure of DNA to ionizing radiation under anoxic conditions. To gain insight into the DNA lesion structural...
Bowman, K K, Sidik, K, Smith, C A, Taylor, J S, Doetsch, P W, Freyer, G A
We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA...
Franklin, W A, Doetsch, P W, Haseltine, W A
Ultraviolet light induces damage to DNA, with the majority of the damage expressed as the formation of cyclobutane dimers and pyrimidine-pyrimidone (6-4) photoproducts. The (6-4) photoproducts have...
Hasegawa, S L, Doetsch, P W, Hamilton, K K, Martin, A M, Okenquist, S A, Lenz, J, ...
A number of eukaryotic DNA binding proteins have been isolated by screening phage expression libraries with DNA probes containing the binding site of the DNA-binding protein. This methodology was...
Doetsch, P W, McCray, W H, Lee, K, Bettler, D R, Valenzuela, M R
A novel endonuclease has been isolated from extracts of spinach leaves (Spinacia oleracea). The enzyme has been purified by a series of column chromatography steps and has a molecular size of...
Doetsch, P W, Chan, G L, Haseltine, W A
Ultraviolet light irradiation of DNA results in the formation of two major types of photoproducts, cyclobutane dimers and 6-4' [pyrimidin-2'-one] -pyrimidine photoproducts. The enzyme T4 DNA...
Doetsch, P W, Suhadolnik, R J, Sawada, Y, Mosca, J D, Flick, M B, Reichenbach, N L, ...
The 3'-deoxyadenosine (cordycepin) analog of (2'-5')oligo(A) [(2'-5')oligoadenylate with a triphosphate at the 5' end], synthesized enzymatically from cordycepin 5'-triphosphate in lysed rabbit...
Doetsch, P W, Henner, W D, Cunningham, R P, Toney, J H, Helland, D E
We have compared the sites of nucleotide incision on DNA damaged by oxidizing agents when cleavage is mediated by either Escherichia coli endonuclease III or an endonuclease present in bovine and...
Substrate specificity of a mammalian DNA repair endonuclease that recognizes oxidative base damage.
Helland, D E, Doetsch, P W, Haseltine, W A
The substrate specificity of a calf thymus endonuclease on DNA damaged by UV ligh, ionizing radiation, and oxidizing agents was investigated. End-labeled DNA fragments of defined sequence were used...
A Drosophila ribosomal protein contains 8-oxoguanine and abasic site DNA repair activities.
Yacoub, A, Augeri, L, Kelley, M R, Doetsch, P W, Deutsch, W A
Ionizing radiation and normal cellular respiration form reactive oxygen species that damage DNA and contribute to a variety of human disorders including tumor promotion and carcinogenesis. A major...
Effects of abasic sites and DNA single-strand breaks on prokaryotic RNA polymerases.
Abasic sites are thought to be the most frequently occurring cellular DNA damage and are generated spontaneously or as the result of chemical or radiation damage to DNA. In contrast to the wealth of...
Lenz, J, Okenquist, S A, LoSardo, J E, Hamilton, K K, Doetsch, P W
Damage to DNA can have lethal or mutagenic consequences for cells unless it is detected and repaired by cellular proteins. Repair depends on the ability of cellular factors to distinguish the damaged...
Dihydrouracil (DHU) is a DNA base damage product produced in significant amounts by ionizing radiation damage to cytosine under anoxic conditions. DHU represents a model for pyrimidine base damage...
Characterization of Schizosaccharomyces pombe Rad2 protein, a FEN-1 homolog.
FEN-1 proteins are a family of nucleases essential for lagging strand DNA synthesis. A gene with sequence similarity to FEN-1 protein-encoding genes, rad2 +, has been identified in...
Sentürker, S, Auffret Van Der Kemp, P, You, H J, Doetsch, P W, Dizdaroglu, M, Boiteux, S
Two genes of Saccharomyces cerevisiae, NTG1 and NTG2, encode proteins with a significant sequence homology to the endonuclease III of Escherichia coli. The Ntg1 and Ntg2 proteins were overexpressed...
Substrate specificity of ultraviolet DNA endonuclease (UVDE/Uve1p) from Schizosaccharomyces pombe.
Avery, A M, Kaur, B, Taylor, J S, Mello, J A, Essigmann, J M, Doetsch, P W
Schizosaccharomyces pombe ultraviolet DNA endonuclease (UVDE or Uve1p) has been shown to cleave 5' to UV light-induced cyclobutane pyrimidine dimers (CPDs) and pyrimidine-pyrimidone (6-4)...
An alternative eukaryotic DNA excision repair pathway.
Freyer, G A, Davey, S, Ferrer, J V, Martin, A M, Beach, D, Doetsch, P W
DNA lesions induced by UV light, cyclobutane pyrimidine dimers, and (6-4)pyrimidine pyrimidones are known to be repaired by the process of nucleotide excision repair (NER). However, in the fission...
RNA polymerase bypass at sites of dihydrouracil: implications for transcriptional mutagenesis.
Dihydrouracil (DHU) is a major base damage product formed from cytosine following exposure of DNA to ionizing radiation under anoxic conditions. To gain insight into the DNA lesion structural...
Bowman, K K, Sidik, K, Smith, C A, Taylor, J S, Doetsch, P W, Freyer, G A
We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA...
Franklin, W A, Doetsch, P W, Haseltine, W A
Ultraviolet light induces damage to DNA, with the majority of the damage expressed as the formation of cyclobutane dimers and pyrimidine-pyrimidone (6-4) photoproducts. The (6-4) photoproducts have...
Hasegawa, S L, Doetsch, P W, Hamilton, K K, Martin, A M, Okenquist, S A, Lenz, J, ...
A number of eukaryotic DNA binding proteins have been isolated by screening phage expression libraries with DNA probes containing the binding site of the DNA-binding protein. This methodology was...
Doetsch, P W, McCray, W H, Lee, K, Bettler, D R, Valenzuela, M R
A novel endonuclease has been isolated from extracts of spinach leaves (Spinacia oleracea). The enzyme has been purified by a series of column chromatography steps and has a molecular size of...
Doetsch, P W, Chan, G L, Haseltine, W A
Ultraviolet light irradiation of DNA results in the formation of two major types of photoproducts, cyclobutane dimers and 6-4' [pyrimidin-2'-one] -pyrimidine photoproducts. The enzyme T4 DNA...
Doetsch, P W, Suhadolnik, R J, Sawada, Y, Mosca, J D, Flick, M B, Reichenbach, N L, ...
The 3'-deoxyadenosine (cordycepin) analog of (2'-5')oligo(A) [(2'-5')oligoadenylate with a triphosphate at the 5' end], synthesized enzymatically from cordycepin 5'-triphosphate in lysed rabbit...
Doetsch, P W, Henner, W D, Cunningham, R P, Toney, J H, Helland, D E
We have compared the sites of nucleotide incision on DNA damaged by oxidizing agents when cleavage is mediated by either Escherichia coli endonuclease III or an endonuclease present in bovine and...
Substrate specificity of a mammalian DNA repair endonuclease that recognizes oxidative base damage.
Helland, D E, Doetsch, P W, Haseltine, W A
The substrate specificity of a calf thymus endonuclease on DNA damaged by UV ligh, ionizing radiation, and oxidizing agents was investigated. End-labeled DNA fragments of defined sequence were used...
A Drosophila ribosomal protein contains 8-oxoguanine and abasic site DNA repair activities.
Yacoub, A, Augeri, L, Kelley, M R, Doetsch, P W, Deutsch, W A
Ionizing radiation and normal cellular respiration form reactive oxygen species that damage DNA and contribute to a variety of human disorders including tumor promotion and carcinogenesis. A major...