Detection of all single-base mismatches in solution by chemiluminescence.
Nelson, N C, Hammond, P W, Matsuda, E, Goud, A A, Becker, M M
A rapid in-solution method for the detection of all 12 single-base mismatches is described. The technique is based on the hybridization protection assay (HPA) format that utilizes oligonucleotide...
dGTP-dependent processivity and possible template switching of euplotes telomerase.
We have measured the processivity of telomeric DNA extension by Euplotes aediculatus telomerase at various concentrations of the nucleotide substrates dGTP and dTTP. The maximum processivity...
Immobilization of acrylamide-modified oligonucleotides by co-polymerization.
Rehman, F N, Audeh, M, Abrams, E S, Hammond, P W, Kenney, M, Boles, T C
A flexible chemistry for solid phase attachment of oligonucleotides is described. Oligonucleotides bearing 5'-terminal acrylamide modifications efficiently co-polymerize with acrylamide monomers to...
Hammond, P W, Lively, T N, Cech, T R
Telomerase is a ribonucleoprotein enzyme that adds telomeric sequence repeats to the ends of linear chromosomes. In vitro, telomerase has been observed to add repeats to a DNA oligonucleotide primer...
Detection of all single-base mismatches in solution by chemiluminescence.
Nelson, N C, Hammond, P W, Matsuda, E, Goud, A A, Becker, M M
A rapid in-solution method for the detection of all 12 single-base mismatches is described. The technique is based on the hybridization protection assay (HPA) format that utilizes oligonucleotide...
dGTP-dependent processivity and possible template switching of euplotes telomerase.
We have measured the processivity of telomeric DNA extension by Euplotes aediculatus telomerase at various concentrations of the nucleotide substrates dGTP and dTTP. The maximum processivity...
Immobilization of acrylamide-modified oligonucleotides by co-polymerization.
Rehman, F N, Audeh, M, Abrams, E S, Hammond, P W, Kenney, M, Boles, T C
A flexible chemistry for solid phase attachment of oligonucleotides is described. Oligonucleotides bearing 5'-terminal acrylamide modifications efficiently co-polymerize with acrylamide monomers to...
Hammond, P W, Lively, T N, Cech, T R
Telomerase is a ribonucleoprotein enzyme that adds telomeric sequence repeats to the ends of linear chromosomes. In vitro, telomerase has been observed to add repeats to a DNA oligonucleotide primer...